##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935674.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307271
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.336806922879155	33.0	31.0	34.0	30.0	34.0
2	32.543741518073624	34.0	31.0	34.0	31.0	34.0
3	32.39208386082644	34.0	31.0	34.0	30.0	34.0
4	36.03362504108751	37.0	35.0	37.0	35.0	37.0
5	36.03288627953826	37.0	35.0	37.0	35.0	37.0
6	36.037169143850214	37.0	35.0	37.0	35.0	37.0
7	36.15721626837547	37.0	37.0	37.0	35.0	37.0
8	35.77229872002239	37.0	35.0	37.0	35.0	37.0
9	37.650738273380824	39.0	38.0	39.0	35.0	39.0
10	37.580331368726625	39.0	37.0	39.0	35.0	39.0
11	37.581991141370324	39.0	37.0	39.0	35.0	39.0
12	37.51338395097487	39.0	37.0	39.0	35.0	39.0
13	37.48374236423223	39.0	37.0	39.0	35.0	39.0
14	38.891584301805246	41.0	38.0	41.0	35.0	41.0
15	38.95373790562728	41.0	38.0	41.0	35.0	41.0
16	39.05729144631287	41.0	39.0	41.0	35.0	41.0
17	38.93697745638215	41.0	38.0	41.0	35.0	41.0
18	38.32747965151283	39.0	38.0	40.0	35.0	41.0
19	37.63026123519629	37.0	37.0	40.0	35.0	41.0
20	36.68815801035568	36.0	35.0	40.0	34.0	41.0
21	36.687656824106405	36.0	35.0	40.0	33.0	41.0
22	36.65312378974912	35.0	35.0	40.0	33.0	41.0
23	36.583279906011306	35.0	35.0	40.0	33.0	41.0
24	36.521230444786525	35.0	35.0	40.0	33.0	41.0
25	36.485685274562194	35.0	35.0	40.0	33.0	41.0
26	36.27437994473934	35.0	35.0	40.0	33.0	41.0
27	36.10536952722515	35.0	35.0	40.0	33.0	41.0
28	35.95196422701785	35.0	35.0	40.0	33.0	41.0
29	35.68989263549115	35.0	35.0	40.0	31.0	41.0
30	35.19050935493424	35.0	35.0	40.0	30.0	41.0
31	34.56389636509791	35.0	35.0	40.0	23.0	41.0
32	33.36038545778808	35.0	33.0	40.0	16.0	41.0
33	32.17274978764674	35.0	31.0	40.0	10.0	41.0
34	31.138600779116807	35.0	25.0	40.0	7.0	41.0
35	30.48316632549118	35.0	23.0	40.0	7.0	41.0
36	30.156597921704293	35.0	21.0	40.0	7.0	41.0
37	29.914030285969062	35.0	20.0	40.0	7.0	41.0
38	29.838858857490617	35.0	20.0	40.0	7.0	41.0
39	29.67893162713045	35.0	18.0	40.0	7.0	41.0
40	29.526362722157312	35.0	18.0	40.0	7.0	41.0
41	29.334486495634145	35.0	18.0	40.0	7.0	41.0
42	29.122917554861996	35.0	18.0	40.0	7.0	41.0
43	29.088007003589667	35.0	18.0	40.0	7.0	41.0
44	29.064636753875245	35.0	18.0	40.0	7.0	41.0
45	29.057760088000496	35.0	18.0	40.0	7.0	41.0
46	29.056777242238937	35.0	18.0	40.0	7.0	41.0
47	28.953005652990356	35.0	18.0	40.0	7.0	41.0
48	28.913245962033514	35.0	18.0	40.0	7.0	41.0
49	28.888642273432897	35.0	18.0	40.0	7.0	41.0
50	28.783803222562494	35.0	18.0	39.0	7.0	41.0
51	28.16876307884571	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	3.0
10	6.0
11	4.0
12	8.0
13	7.0
14	8.0
15	23.0
16	35.0
17	90.0
18	191.0
19	419.0
20	740.0
21	1367.0
22	2075.0
23	3309.0
24	5713.0
25	10272.0
26	16708.0
27	20511.0
28	17924.0
29	13650.0
30	10258.0
31	8953.0
32	8586.0
33	9316.0
34	13866.0
35	19914.0
36	21389.0
37	20725.0
38	36624.0
39	64497.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.292477975467907	11.577076912562525	13.032795154765664	45.0976499572039
2	56.67960855401258	19.91629538745928	13.233920545707209	10.17017551282093
3	17.88746741475766	20.781004390261366	51.41812927350775	9.913398921473227
4	17.450068506302255	14.92168151241087	56.65747825209668	10.970771729190194
5	20.31984795180801	16.857106593202744	50.39753182044514	12.4255136345441
6	15.233458412931908	25.11854356577744	50.64649771699901	9.001500304291651
7	45.554901048260334	4.093780408824784	47.15348991606758	3.197828626847311
8	45.09732451158749	8.61422002076343	42.5210970120838	3.7673584555652826
9	41.59878413517709	5.051892303536618	43.4434098889905	9.90591367229579
10	27.137608169986756	15.362660322646784	48.112578147628646	9.387153359737821
11	21.821454025924997	15.644170780841668	50.70182347178874	11.832551721444588
12	17.90081068503048	13.203328657764644	53.59405866482682	15.301801992378063
13	17.26391361371558	14.602419362712393	55.460489274939704	12.673177748632314
14	12.438531459200512	20.48582521617725	52.80582938188114	14.269813942741097
15	10.99160024864045	16.374796189682723	58.00937934266494	14.624224219011882
16	12.218530222507168	16.07994246121502	55.000634618952006	16.700892697325813
17	12.203234278535884	18.25749908061613	54.45486232023198	15.084404320616004
18	12.749332022872316	16.843437877313512	53.77370464508529	16.633525454728886
19	14.037445772624165	16.873704319639668	52.48689267779908	16.601957229937092
20	17.714655792443804	16.16293109339964	54.22184325888223	11.900569855274334
21	15.097096699656005	19.126764322047965	53.45509338661964	12.321045591676402
22	13.684988170051843	15.615531566597562	55.22909744167202	15.470382821678582
23	15.15209700882934	17.88909464283971	53.55077439784425	13.408033950486702
24	14.645703629694959	17.07027347195147	53.321660683891416	14.962362214462152
25	13.392412560899011	20.290883291947498	51.971386821405204	14.34531732574828
26	13.214719254339002	17.331606301928918	54.14015640916324	15.313518034568832
27	15.95985302875963	18.172883220349465	52.07487852742367	13.792385223467232
28	12.523147319467181	19.056142623286938	53.85897139658478	14.561738660661112
29	15.378281712234475	16.195475655040664	53.33370217169859	15.09254046102626
30	14.325139697530846	20.26972932688083	51.27851310406775	14.12661787152058
31	16.02168769587758	18.281907501846902	47.79396688916299	17.90243791311253
32	17.814242151065347	21.820803134692177	46.35159191723267	14.013362797009806
33	17.835070670515606	21.672725379225504	43.519238717614094	16.9729652326448
34	18.885934565904364	21.939265339065518	41.90112311282223	17.27367698220789
35	18.49312170689717	23.573978670294302	40.46883695500064	17.464062667807895
36	18.685785511812046	26.976838035480082	37.76438388263129	16.57299257007658
37	16.84181064923146	27.682729577473957	39.243208763599554	16.232251009695027
38	17.320541150970968	25.205762990975394	37.144409983369734	20.32928587468391
39	19.564163230503368	26.585001513322116	37.032131245708186	16.81870401046633
40	19.318451790113613	23.8750158654738	39.49054743207137	17.31598491234122
41	17.308174217547375	26.592161316883146	37.370594686774865	18.729069778794617
42	17.02113118387352	25.33724302000514	39.690045594930865	17.95158020119048
43	18.84525386385308	26.53293021469647	36.84695268997074	17.774863231479703
44	18.3134757266387	24.80579032840717	38.115214257121565	18.765519687832565
45	17.53110446478841	23.353001096751726	37.528435810733846	21.587458627726015
46	19.297297825046947	25.54032108464515	37.67456089250206	17.487820197805846
47	16.68266774280684	24.366764191869716	42.151065346225316	16.799502719098125
48	17.086545752771983	23.547617575365067	40.01581665695754	19.350020014905407
49	16.086776819159635	21.99491653947167	43.278083515854085	18.64022312551461
50	16.531986422408885	22.165124596854245	41.90112311282223	19.40176586791464
51	15.92340311972168	22.36852810711066	39.88531296477702	21.82275580839064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	179.5
2	274.0
3	18258.5
4	36243.0
5	24267.5
6	12292.0
7	11730.5
8	11169.0
9	10648.0
10	10127.0
11	9692.5
12	9258.0
13	8700.5
14	8143.0
15	7484.0
16	6825.0
17	6282.0
18	5739.0
19	5127.0
20	4515.0
21	4128.0
22	3741.0
23	3454.5
24	3168.0
25	2994.0
26	2735.5
27	2651.0
28	2783.0
29	2915.0
30	3340.0
31	3765.0
32	3963.5
33	4162.0
34	4437.0
35	4712.0
36	5167.0
37	5622.0
38	6112.5
39	6603.0
40	7259.5
41	7916.0
42	8476.5
43	9037.0
44	10567.0
45	12097.0
46	19015.0
47	25933.0
48	22405.0
49	18877.0
50	18595.5
51	18314.0
52	15803.5
53	13293.0
54	12477.0
55	11661.0
56	10789.5
57	9918.0
58	9137.0
59	8356.0
60	8148.0
61	7940.0
62	7240.5
63	6541.0
64	5433.5
65	4326.0
66	3505.0
67	2684.0
68	2329.0
69	1974.0
70	1727.0
71	1480.0
72	1225.5
73	971.0
74	755.0
75	432.5
76	326.0
77	237.5
78	149.0
79	99.5
80	50.0
81	48.0
82	46.0
83	27.5
84	9.0
85	5.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	307271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.11433818138803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.94700901874423	36.518707396434934
2	7.062485505083314	6.372387189549593
3	2.6067683002784623	3.5280787997785374
4	1.5557387656830672	2.807444991876843
5	1.1457067480658631	2.5843900844470835
6	0.9937672035469459	2.689988981663351
7	0.8512996143700648	2.6884073087463425
8	0.7748202112402554	2.796440083173391
9	0.6338872492638549	2.573766335894342
>10	3.3261414189311314	22.05218220093254
>50	0.07260706722895167	2.2490405446046267
>100	0.023234261513264538	2.266401329466853
>500	0.002904282689158067	0.8926063915012536
>1k	0.002904282689158067	3.065980119064857
>5k	0.0	0.0
>10k+	7.260706722895168E-4	6.91417824286549
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21108	6.869506071187974	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGC	3297	1.0729941973046595	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTC	3015	0.9812185334769633	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCG	1959	0.6375479625477185	No Hit
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	1089	0.35441027627078375	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTT	797	0.25938015627898503	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAGGTAT	779	0.2535221351836002	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCT	577	0.18778212066872565	No Hit
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	572	0.1861548925866743	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	484	0.15751567834257058	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	468	0.15230854848000625	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTC	452	0.14710141861744191	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAGGTATCGTA	411	0.13375814834462085	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	393	0.12790012724923602	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	380	0.12366933423590251	No Hit
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	375	0.12204210615385117	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12659834478359494	0.0
2	0.0	0.0	0.0	0.7869275004800322	0.0
3	0.0	0.0	0.0	1.0437040918277352	0.0
4	0.0	0.0	0.0	1.451487449189803	0.0
5	0.0	0.0	0.0	3.1320886123324363	0.0
6	0.0	0.0	0.0	3.76442944501759	0.0
7	0.0	0.0	0.0	4.312479863052484	0.0
8	0.0	0.0	0.0	5.006004471622769	0.0
9	0.0	0.0	0.0	5.208106199413547	0.0
10	0.0	0.0	0.0	6.3214556531530794	0.0
11	0.0	0.0	0.0	7.049152051446443	0.0
12	0.0	0.0	0.0	8.754487081436256	0.0
13	0.0	0.0	0.0	9.080258143462936	0.0
14	0.0	0.0	0.0	9.243957288517302	0.0
15	0.0	0.0	0.0	9.586651522597316	0.0
16	3.254456164102698E-4	0.0	0.0	10.032186571462976	0.0
17	3.254456164102698E-4	0.0	0.0	10.483254195807609	0.0
18	3.254456164102698E-4	0.0	0.0	10.923256669194295	0.0
19	3.254456164102698E-4	0.0	0.0	11.515893136677395	0.0
20	3.254456164102698E-4	0.0	0.0	11.838084296923563	0.0
21	3.254456164102698E-4	0.0	0.0	12.196074474974859	0.0
22	3.254456164102698E-4	0.0	0.0	12.617851993842569	0.0
23	3.254456164102698E-4	0.0	0.0	12.998297919426173	0.0
24	3.254456164102698E-4	0.0	0.0	13.311051156796443	0.0
25	3.254456164102698E-4	0.0	0.0	13.578242007869274	0.0
26	3.254456164102698E-4	0.0	0.0	13.8864390066098	0.0
27	3.254456164102698E-4	0.0	0.0	14.17576015959853	0.0
28	3.254456164102698E-4	0.0	0.0	14.44034744574008	0.0
29	3.254456164102698E-4	0.0	0.0	14.700703938868296	0.0
30	3.254456164102698E-4	0.0	0.0	14.98319073391241	0.0
31	3.254456164102698E-4	0.0	0.0	15.266328420189344	0.0
32	3.254456164102698E-4	0.0	0.0	15.534495608111406	0.0
33	3.254456164102698E-4	0.0	0.0	15.837160031372957	0.0
34	3.254456164102698E-4	0.0	0.0	16.122250391348352	0.0
35	3.254456164102698E-4	0.0	0.0	16.405713523241698	0.0
36	3.254456164102698E-4	0.0	0.0	16.706750718421198	0.0
37	3.254456164102698E-4	0.0	0.0	16.97361612387762	0.0
38	3.254456164102698E-4	0.0	0.0	17.240806974950452	0.0
39	3.254456164102698E-4	0.0	0.0	17.497909011914565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCA	45	3.8198777E-10	45.000004	5
CGCACGG	35	1.2080454E-7	45.000004	2
TGCACGG	30	2.1602918E-6	45.000004	2
TGGACGG	25	3.8841128E-5	45.0	2
TACGGCC	20	7.025211E-4	45.0	7
ACGGGTC	20	7.025211E-4	45.0	5
CCTCGAT	20	7.025211E-4	45.0	15
TACGCGG	20	7.025211E-4	45.0	2
TACGAAT	20	7.025211E-4	45.0	12
CGAATAT	20	7.025211E-4	45.0	14
TCGTAAG	20	7.025211E-4	45.0	42
GCTACGG	25	3.8841128E-5	45.0	2
AGCTACG	20	7.025211E-4	45.0	9
ATGGGCT	55	1.8189894E-12	44.999996	5
CGTTTTT	11415	0.0	44.36925	1
GATGAAT	475	0.0	44.05263	20
ATGAATG	470	0.0	43.085106	21
CGATGAA	510	0.0	42.35294	19
CCGATGA	505	0.0	42.326733	18
ACCGATG	505	0.0	42.326733	17
>>END_MODULE