##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935673.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533496
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.531160496048706	34.0	31.0	34.0	31.0	34.0
2	32.73467654865266	34.0	31.0	34.0	31.0	34.0
3	32.557616177065995	34.0	31.0	34.0	31.0	34.0
4	36.14943129845397	37.0	37.0	37.0	35.0	37.0
5	36.1772478144166	37.0	37.0	37.0	35.0	37.0
6	36.1906199859043	37.0	37.0	37.0	35.0	37.0
7	36.31315323826233	37.0	37.0	37.0	35.0	37.0
8	36.084673549567384	37.0	37.0	37.0	35.0	37.0
9	37.90151003943797	39.0	38.0	39.0	35.0	39.0
10	37.7817640619611	39.0	38.0	39.0	35.0	39.0
11	37.7651416318023	39.0	38.0	39.0	35.0	39.0
12	37.69272122002789	39.0	38.0	39.0	35.0	39.0
13	37.68959279919625	39.0	38.0	39.0	35.0	39.0
14	39.14331878776973	41.0	39.0	41.0	35.0	41.0
15	39.227163840028794	41.0	39.0	41.0	35.0	41.0
16	39.258183753955045	41.0	39.0	41.0	35.0	41.0
17	39.204681947006165	41.0	39.0	41.0	35.0	41.0
18	38.4804103498433	39.0	39.0	40.0	35.0	41.0
19	37.72081140252223	37.0	37.0	40.0	35.0	41.0
20	36.84919474560259	35.0	35.0	40.0	34.0	41.0
21	36.80382983190127	35.0	35.0	40.0	34.0	41.0
22	36.79484757149069	35.0	35.0	40.0	34.0	41.0
23	36.801505540810055	35.0	35.0	40.0	34.0	41.0
24	36.62690629358046	35.0	35.0	40.0	34.0	41.0
25	36.54180912321742	35.0	35.0	40.0	34.0	41.0
26	36.37069256376805	35.0	35.0	40.0	33.0	41.0
27	36.29122242715972	35.0	35.0	40.0	33.0	41.0
28	36.12541612308246	35.0	35.0	40.0	33.0	41.0
29	35.79872951249869	35.0	35.0	40.0	33.0	41.0
30	35.217092536776285	35.0	35.0	40.0	30.0	41.0
31	34.4418046245895	35.0	35.0	40.0	22.0	41.0
32	33.23417607629673	35.0	33.0	40.0	15.0	41.0
33	31.953364223911706	35.0	31.0	40.0	10.0	41.0
34	30.868220192841182	35.0	23.0	40.0	7.0	41.0
35	30.227568716541455	35.0	21.0	40.0	7.0	41.0
36	29.85011508989758	35.0	20.0	40.0	7.0	41.0
37	29.64670775413499	35.0	18.0	40.0	7.0	41.0
38	29.470764541814745	35.0	18.0	40.0	7.0	41.0
39	29.3289846596788	35.0	18.0	40.0	7.0	41.0
40	29.214125316778382	35.0	18.0	40.0	7.0	41.0
41	29.057288152113607	35.0	18.0	40.0	7.0	41.0
42	28.93608199499153	35.0	18.0	40.0	7.0	41.0
43	28.80605477829262	35.0	18.0	40.0	7.0	41.0
44	28.672158741583818	35.0	18.0	40.0	7.0	41.0
45	28.614336377404893	35.0	18.0	40.0	7.0	41.0
46	28.58069226385952	35.0	18.0	40.0	7.0	41.0
47	28.60858563138243	35.0	18.0	40.0	7.0	41.0
48	28.581550752020632	35.0	18.0	40.0	7.0	41.0
49	28.56185238502257	35.0	18.0	39.0	7.0	41.0
50	28.471388726438438	35.0	18.0	39.0	7.0	41.0
51	27.897285078051194	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	6.0
9	7.0
10	12.0
11	12.0
12	8.0
13	13.0
14	19.0
15	36.0
16	70.0
17	141.0
18	287.0
19	547.0
20	896.0
21	1491.0
22	2383.0
23	4078.0
24	8234.0
25	17335.0
26	31001.0
27	37946.0
28	35104.0
29	26334.0
30	19479.0
31	15929.0
32	14161.0
33	15101.0
34	24255.0
35	34728.0
36	34952.0
37	34053.0
38	61205.0
39	113552.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.312272256961627	12.513308440925519	13.397851155397603	49.77656814671525
2	61.53616896846462	14.345187217898541	13.932250663547618	10.186393150089222
3	19.85825573200174	14.317445679067884	55.57173811987344	10.252560469056938
4	18.139030095820775	15.175371511688935	55.21522185733352	11.470376535156777
5	15.209673549567382	17.75420996596038	54.336114984929594	12.70000149954264
6	15.961319297614226	20.480753370222082	54.63621095565853	8.921716376505167
7	47.65284088353052	3.7269257876347717	45.429581477649315	3.1906518511853887
8	47.55180919819455	3.777347908887789	45.55348118823759	3.117361704680073
9	43.87080690389431	5.083636990717831	46.693508479913625	4.35204762547423
10	24.803559914226163	15.073590055033215	50.73402612203278	9.388823908707844
11	18.9231034534467	15.2707799121268	53.92823938698697	11.87787724743953
12	18.157024607494716	13.099067284478233	56.354311934859865	12.389596173167185
13	15.507145320677193	13.910132409615066	57.22929506500517	13.353427204702568
14	12.752298049095026	15.80367987763732	57.20980101069174	14.234221062575914
15	12.422211225576199	15.25222307196305	59.53071813097005	12.794847571490694
16	14.191671540180245	14.736005518316913	58.42780451962152	12.644518421881326
17	13.837966920089373	15.152690929266575	56.682711772909265	14.326630377734793
18	14.629537990912771	15.570500997195854	56.54981480648402	13.25014620540735
19	15.480340995996222	16.38437776478174	55.46995666321772	12.66532457600432
20	16.861044881311198	15.459159956213355	56.03284748151813	11.646947680957307
21	15.9245805029466	15.431980745872508	56.2467197504761	12.396719000704785
22	14.465900400377885	14.960936914241158	56.5151378829457	14.058024802435257
23	14.952501986894	15.117264234408506	56.68064990177996	13.249583876917539
24	15.092709223686777	14.040780062081065	57.044288991857485	13.822221722374675
25	14.771994541664792	14.82597807668661	55.62103558414684	14.78099179750176
26	13.518939223536822	16.65916895346919	55.82872223971689	13.993169583277101
27	14.146122932505586	16.131517387196904	56.17811567471921	13.5442440055783
28	13.303192526279483	16.964513323436353	56.203982785250496	13.528311365033666
29	14.320257321516937	16.291781006792927	55.597417787574784	13.790543884115344
30	15.249411429513998	15.764129440520641	54.90387931680837	14.082579813156986
31	15.522515632732018	17.81662842832936	52.24743953094306	14.41341640799556
32	16.248294270247573	19.359470361539728	50.51190636855759	13.880328999655106
33	17.131524884910103	21.016464978181652	46.7396194160781	15.112390720830145
34	16.52083614497578	23.047220597717697	44.78571535681617	15.64622790049035
35	17.014748001859434	24.235983025177323	42.10115914646033	16.648109826502917
36	17.92796946931186	26.31359935219758	39.64078456070899	16.117646617781574
37	18.793205572300447	26.296354611843388	39.734693418507355	15.175746397348808
38	18.120473255657025	25.614812482192935	39.32962946301378	16.935084799136263
39	18.647937379099375	25.09409630062831	39.103385967279976	17.154580352992337
40	18.5219757973818	25.191379129365544	39.78567786825018	16.500967205002475
41	16.477349408430428	26.577518856748693	39.26046305876707	17.684668676053803
42	17.51053428704245	26.35371211780407	38.38435527164215	17.75139832351133
43	18.08598377494864	25.16607434732407	38.791855983924904	17.95608589380239
44	17.803319987403842	25.49035044311485	38.537870949360446	18.168458620120866
45	18.024689969559287	24.345262195030514	39.13675079100874	18.49329704440146
46	17.367890293460494	24.94807833610749	39.670025642179134	18.014005728252883
47	16.87828962166539	24.311335042811944	41.45017019808958	17.360205137433084
48	17.333025927092237	22.801857933330336	42.089725133834186	17.77539100574325
49	17.021870829397034	22.49445169223387	42.41943707169313	18.064240406675964
50	15.986061751165895	22.2205977176961	42.937716496468575	18.855624034669425
51	15.966942582512333	22.29013900760268	41.99469161905619	19.748226790828795
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	339.0
2	555.0
3	34295.0
4	68035.0
5	46245.0
6	24455.0
7	23090.5
8	21726.0
9	20999.0
10	20272.0
11	19199.0
12	18126.0
13	17009.5
14	15893.0
15	14441.5
16	12990.0
17	11941.5
18	10893.0
19	9895.0
20	8897.0
21	7984.0
22	7071.0
23	6363.0
24	5655.0
25	5280.0
26	4889.0
27	4873.0
28	4839.5
29	4806.0
30	5214.0
31	5622.0
32	6214.0
33	6806.0
34	7753.5
35	8701.0
36	8862.5
37	9024.0
38	9573.0
39	10122.0
40	11656.0
41	13190.0
42	14457.0
43	15724.0
44	17427.5
45	19131.0
46	21141.5
47	23152.0
48	24919.5
49	26687.0
50	27283.0
51	27879.0
52	25128.0
53	22377.0
54	20789.5
55	19202.0
56	18570.5
57	17939.0
58	17650.0
59	17361.0
60	17091.0
61	16821.0
62	15540.0
63	14259.0
64	12178.0
65	10097.0
66	8474.0
67	6851.0
68	5800.5
69	4750.0
70	4036.0
71	3322.0
72	2866.0
73	2410.0
74	1878.0
75	1047.0
76	748.0
77	534.0
78	320.0
79	267.0
80	214.0
81	158.0
82	102.0
83	75.5
84	49.0
85	30.0
86	11.0
87	6.0
88	1.0
89	0.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.42353363941134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.44791169870823	35.73780511567958
2	6.979866804936326	6.201406956153988
3	2.6720187064918663	3.5610153867893337
4	1.6004321084705744	2.843873984329463
5	1.2377995905673382	2.7493715875208866
6	0.9665538174896342	2.5762641615331328
7	0.820635574020267	2.5518872419730876
8	0.726023487331089	2.5802023049964293
9	0.617352272481001	2.4682472499534103
>10	3.8045071624775098	25.53376052770886
>50	0.07894037634601511	2.46287446731171
>100	0.04413870505079618	3.069967461475597
>500	0.0033952850039073983	0.9668238741292674
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.244106254884248E-4	6.696499680445266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35518	6.657594433697723	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	831	0.15576499167753835	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	720	0.13495883755454585	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	702	0.1315848666156822	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	628	0.1177140972003539	Illumina PCR Primer Index 5 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	619	0.11602711173092208	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	571	0.10702985589395235	RNA PCR Primer, Index 33 (96% over 27bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	553	0.1036558849550887	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.623284898106077E-4	0.0	0.0	0.07029106122632597	0.0
2	5.623284898106077E-4	0.0	0.0	0.3606400047985365	0.0
3	5.623284898106077E-4	0.0	0.0	0.5201538530748122	0.0
4	5.623284898106077E-4	0.0	0.0	0.7087213399913026	0.0
5	5.623284898106077E-4	0.0	0.0	1.2926057552446504	0.0
6	5.623284898106077E-4	0.0	0.0	1.6376880051584266	0.0
7	5.623284898106077E-4	0.0	0.0	1.9790213984734657	0.0
8	5.623284898106077E-4	0.0	0.0	2.449877787274881	0.0
9	5.623284898106077E-4	0.0	0.0	2.633571760613013	0.0
10	5.623284898106077E-4	0.0	0.0	3.033387316868355	0.0
11	5.623284898106077E-4	0.0	0.0	3.6633826682861725	0.0
12	5.623284898106077E-4	0.0	0.0	4.169853194775594	0.0
13	5.623284898106077E-4	0.0	0.0	4.38916130580173	0.0
14	5.623284898106077E-4	0.0	0.0	4.482132949450418	0.0
15	5.623284898106077E-4	0.0	0.0	4.668076236747792	0.0
16	5.623284898106077E-4	0.0	0.0	5.0071603161035885	0.0
17	5.623284898106077E-4	0.0	0.0	5.395354416902845	0.0
18	5.623284898106077E-4	0.0	0.0	5.819162655390106	0.0
19	5.623284898106077E-4	0.0	0.0	6.092829187097935	0.0
20	5.623284898106077E-4	0.0	0.0	6.3646212905063955	0.0
21	5.623284898106077E-4	0.0	0.0	6.698082084964086	0.0
22	5.623284898106077E-4	0.0	0.0	7.068844002579214	1.8744282993686927E-4
23	5.623284898106077E-4	0.0	0.0	7.42704725058857	1.8744282993686927E-4
24	5.623284898106077E-4	0.0	0.0	7.704650081725074	1.8744282993686927E-4
25	5.623284898106077E-4	0.0	0.0	7.94795087498313	1.8744282993686927E-4
26	5.623284898106077E-4	0.0	0.0	8.183566512213774	1.8744282993686927E-4
27	7.497713197474771E-4	0.0	0.0	8.421056577743789	1.8744282993686927E-4
28	7.497713197474771E-4	0.0	0.0	8.668293670430518	1.8744282993686927E-4
29	7.497713197474771E-4	0.0	0.0	8.91609309160706	1.8744282993686927E-4
30	7.497713197474771E-4	0.0	0.0	9.186573095205961	1.8744282993686927E-4
31	7.497713197474771E-4	0.0	0.0	9.456865655974928	1.8744282993686927E-4
32	7.497713197474771E-4	0.0	0.0	9.706352062620901	1.8744282993686927E-4
33	7.497713197474771E-4	0.0	0.0	9.94759098474965	1.8744282993686927E-4
34	7.497713197474771E-4	0.0	0.0	10.182081965000675	1.8744282993686927E-4
35	7.497713197474771E-4	0.0	0.0	10.432130700136458	1.8744282993686927E-4
36	7.497713197474771E-4	0.0	0.0	10.684053863571611	1.8744282993686927E-4
37	7.497713197474771E-4	0.0	0.0	10.938413783795943	1.8744282993686927E-4
38	7.497713197474771E-4	0.0	0.0	11.193710918169959	1.8744282993686927E-4
39	7.497713197474771E-4	0.0	0.0	11.452382023482837	1.8744282993686927E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8879847E-5	45.0	1
TTATCGG	20	7.0298836E-4	45.0	30
TAGCCGT	20	7.0298836E-4	45.0	44
GCGATAT	20	7.0298836E-4	45.0	9
AATTACG	25	3.8879847E-5	45.0	1
CGGTCTA	20	7.0298836E-4	45.0	31
ATTATCG	20	7.0298836E-4	45.0	30
GTAACGG	35	1.2102828E-7	45.0	2
TGCGTAG	20	7.0298836E-4	45.0	1
CGTTTTT	21995	0.0	44.49875	1
GGCGATA	70	0.0	41.785713	8
ACGGGAT	60	3.6379788E-12	41.249996	5
TACGGGA	110	0.0	40.909092	4
GTTTTTT	24425	0.0	40.172977	2
CTAGGGT	45	1.9250365E-8	40.0	4
GCTACGA	85	0.0	39.705883	10
CGGGTAT	40	3.4535697E-7	39.375	6
ATTACGG	40	3.4535697E-7	39.375	2
ACGGGTC	40	3.4535697E-7	39.375	5
GCAATAG	35	6.2419967E-6	38.571426	1
>>END_MODULE