SRR2935670.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156329
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9392	6.007842434864933	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	1317	0.8424540552296758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	1176	0.7522596575171593	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	1063	0.6799762040312418	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	893	0.5712311855126049	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	534	0.3415872934644244	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	385	0.24627548311573672	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTACTCCT	305	0.19510135675402518	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	265	0.1695142935731694	No Hit
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	217	0.13880981775614248	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTA	207	0.13241305196092854	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	191	0.12217822668858624	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	189	0.12089887352954345	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	186	0.11897984379097928	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	173	0.11066404825720115	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	172	0.11002437167767977	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	164	0.10490695904150861	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	163	0.10426728246198723	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAG	20	7.014574E-4	45.000004	1
GATACCA	20	7.014574E-4	45.000004	9
CCGGGAT	20	7.014574E-4	45.000004	5
AACCCGC	20	7.014574E-4	45.000004	21
TCCGAAC	20	7.014574E-4	45.000004	17
TGGGTGC	35	1.2029341E-7	45.000004	6
GCTATTA	20	7.014574E-4	45.000004	9
CGTTCTT	20	7.014574E-4	45.000004	1
TGCGGGA	35	1.2029341E-7	45.000004	4
ACTTAGG	20	7.014574E-4	45.000004	2
GCGCAGG	20	7.014574E-4	45.000004	2
GTAATGG	20	7.014574E-4	45.000004	2
TAGCACG	20	7.014574E-4	45.000004	1
CTTATTA	20	7.014574E-4	45.000004	19
GCGGGCA	20	7.014574E-4	45.000004	5
CGGGATT	40	6.7502697E-9	45.000004	6
GCGATTG	30	2.1534397E-6	45.000004	9
GTTCCGA	20	7.014574E-4	45.000004	15
ATGAACA	20	7.014574E-4	45.000004	34
GATTGGG	40	6.7502697E-9	45.000004	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content