##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156329
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28805276052428	33.0	31.0	34.0	30.0	34.0
2	32.4862309616258	34.0	31.0	34.0	30.0	34.0
3	32.36819144240672	34.0	31.0	34.0	30.0	34.0
4	36.00773368984641	37.0	35.0	37.0	35.0	37.0
5	36.03417791964383	37.0	35.0	37.0	35.0	37.0
6	36.04748319249787	37.0	35.0	37.0	35.0	37.0
7	36.19544678210697	37.0	37.0	37.0	35.0	37.0
8	35.87307537309137	37.0	36.0	37.0	35.0	37.0
9	37.79308381682222	39.0	38.0	39.0	35.0	39.0
10	37.62000652470111	39.0	37.0	39.0	35.0	39.0
11	37.594943996315465	39.0	37.0	39.0	35.0	39.0
12	37.510832922874194	39.0	37.0	39.0	35.0	39.0
13	37.41965981999501	39.0	37.0	39.0	35.0	39.0
14	38.76715772505421	40.0	38.0	41.0	35.0	41.0
15	38.85142871764036	41.0	38.0	41.0	35.0	41.0
16	38.98480128447057	41.0	38.0	41.0	35.0	41.0
17	38.91879945499556	41.0	38.0	41.0	35.0	41.0
18	38.33240793454829	39.0	38.0	40.0	35.0	41.0
19	37.67496753641359	37.0	37.0	40.0	35.0	41.0
20	36.84626652764362	36.0	35.0	40.0	34.0	41.0
21	36.85944386518177	36.0	35.0	40.0	34.0	41.0
22	36.85248418399657	36.0	35.0	40.0	34.0	41.0
23	36.824677443084774	36.0	35.0	40.0	34.0	41.0
24	36.68605952830249	36.0	35.0	40.0	34.0	41.0
25	36.657235701629254	36.0	35.0	40.0	33.0	41.0
26	36.48326925906262	35.0	35.0	40.0	33.0	41.0
27	36.3662084450102	35.0	35.0	40.0	33.0	41.0
28	36.22505101420722	35.0	35.0	40.0	33.0	41.0
29	35.992227929558815	36.0	35.0	40.0	33.0	41.0
30	35.51371786424784	36.0	35.0	40.0	31.0	41.0
31	34.86324354406412	35.0	35.0	40.0	25.0	41.0
32	33.77688080906294	35.0	34.0	40.0	18.0	41.0
33	32.67285660370117	35.0	33.0	40.0	12.0	41.0
34	31.77453959278189	35.0	30.0	40.0	8.0	41.0
35	31.27356408599812	35.0	27.0	40.0	7.0	41.0
36	30.938623032194922	35.0	24.0	40.0	7.0	41.0
37	30.770093840554217	35.0	23.0	40.0	7.0	41.0
38	30.648951889924454	35.0	23.0	40.0	7.0	41.0
39	30.536029783341544	35.0	23.0	40.0	7.0	41.0
40	30.442349148270633	35.0	23.0	40.0	7.0	41.0
41	30.248789412073254	35.0	22.0	40.0	7.0	41.0
42	30.130161390401014	35.0	21.0	40.0	7.0	41.0
43	30.003614172674297	35.0	21.0	40.0	7.0	41.0
44	29.892425589621887	35.0	20.0	40.0	7.0	41.0
45	29.828860927914846	35.0	20.0	40.0	7.0	41.0
46	29.81103953840938	35.0	20.0	40.0	7.0	41.0
47	29.771494732263367	35.0	20.0	40.0	7.0	41.0
48	29.76397853245399	35.0	20.0	40.0	7.0	41.0
49	29.75332791740496	35.0	20.0	40.0	7.0	41.0
50	29.600797037018083	35.0	20.0	40.0	7.0	41.0
51	28.962387017124144	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	2.0
11	1.0
12	1.0
13	3.0
14	2.0
15	5.0
16	18.0
17	25.0
18	61.0
19	138.0
20	257.0
21	441.0
22	738.0
23	1347.0
24	2610.0
25	5063.0
26	8428.0
27	9609.0
28	8378.0
29	6128.0
30	4726.0
31	3972.0
32	3956.0
33	4645.0
34	7504.0
35	11937.0
36	10777.0
37	10596.0
38	19309.0
39	35614.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.9818267883758	12.955369765046793	15.01960608716233	43.04319735941508
2	54.306622571627784	18.48473411842972	15.61514498269675	11.593498327245745
3	19.311835935750885	18.428442579431838	51.08329228741948	11.176429197397795
4	18.005616360368197	15.4641813099297	54.51579681313128	12.014405516570822
5	18.53079083215526	17.445259676707455	50.524854633497306	13.499094857639976
6	15.78082121679279	23.917507308304923	50.25299208720071	10.04867938770158
7	48.00772729308062	3.8207882094812864	44.01294705396951	4.158537443468583
8	47.35461750538927	6.092279743361757	41.47535006300814	5.0777526882408255
9	45.02939313882901	4.086893666562187	42.483480352333856	8.400232842274946
10	27.24318584523665	14.423427515048392	47.54716015582521	10.786226483889745
11	21.067108469957592	15.179525232042678	50.85236904221225	12.900997255787475
12	18.25445054980202	13.53171836319557	53.43922112979678	14.774609957205637
13	15.820481164723116	16.05588214598699	55.222639433502415	12.900997255787475
14	11.980502657856187	20.781812715491046	52.61403834221417	14.623646284438587
15	10.62310895611179	16.05588214598699	59.72788158307159	13.593127314829623
16	12.223579758074319	16.026457023329005	55.323708333066804	16.426254885529875
17	12.130826654043716	16.746732851870092	53.8972295607341	17.2252109333521
18	13.156867887596032	16.08082953258832	55.115173768142824	15.647128811672816
19	14.063929277357367	17.37361589980106	52.62811122696365	15.934343595877923
20	15.51983317234806	17.458692884877404	53.84605543437239	13.175418508402151
21	14.010836121257093	19.068758835532755	53.58314836018909	13.337256683021065
22	12.473053624087662	16.030934759385655	53.31832225626724	18.177689360259453
23	12.814640917552087	17.988984769300643	53.315763549949146	15.880610763198128
24	14.809152492499791	15.73476450306725	53.01191717467648	16.444165829756475
25	12.323369304479655	18.918434839345228	52.090143223586146	16.66805263258896
26	12.32976607027487	17.481721241740175	54.24265491367565	15.94585777430931
27	15.293387663197489	18.10156784729641	51.98587594112417	14.61916854838194
28	11.116299598922785	18.020968598276713	55.6972794555073	15.16545234729321
29	12.77498096962176	16.3283843688631	54.75887391334942	16.13776074816573
30	15.040715414286538	17.848895598385457	51.7114546885095	15.398934298818517
31	14.237281630407667	17.958280293483615	50.9316889380729	16.872749138035807
32	15.877412380300521	20.47476795732078	47.22348380658739	16.424335855791313
33	15.890205911890948	19.289447255467636	43.58692245200827	21.23342438063315
34	14.401678511344665	20.42551286069763	46.04008213447281	19.132726493484896
35	15.943938744570746	21.070946529434718	40.91819176224501	22.066922963749526
36	17.506668628341508	21.610833562550773	41.644864356581316	19.237633452526403
37	16.968060948384498	23.284867171158265	42.055536720634045	17.691535159823193
38	15.633055926923348	24.612196073665153	37.94241631431149	21.812331685100013
39	16.437769063961262	23.909831189350665	40.41028855810502	19.242111188583053
40	17.133737182480537	21.953700209174244	39.656749547428824	21.255813060916402
41	15.741800945441984	22.970785970613257	38.054999392307245	23.232413691637507
42	17.168919394354216	21.99975692289978	38.98572881551088	21.84559486723513
43	18.7591553710444	22.167991863313908	37.88548509873408	21.187367666907612
44	17.154206833025224	23.227296279001337	38.307031964638675	21.31146492333476
45	16.903453613852836	22.819182621266688	38.12344478631604	22.153918978564437
46	19.17622450089235	22.97654305982895	38.31598743675198	19.531245002526724
47	14.866083708077197	21.652412540219665	43.05151315494886	20.42999059675428
48	16.259299298274794	21.048557849151468	40.56572996692872	22.126412885645017
49	16.39043299707668	19.178783207210433	43.31953764176832	21.111246153944567
50	16.14863525001759	19.884346474422532	41.3845159887161	22.582502286843773
51	15.333687287707335	20.307812370065694	38.87698379699224	25.48151654523473
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	108.0
2	141.0
3	7900.5
4	15660.0
5	10291.5
6	4923.0
7	4925.5
8	4928.0
9	4953.0
10	4978.0
11	4859.0
12	4740.0
13	4480.0
14	4220.0
15	3979.5
16	3739.0
17	3417.5
18	3096.0
19	2762.5
20	2429.0
21	2180.5
22	1932.0
23	1708.0
24	1484.0
25	1389.5
26	1335.0
27	1375.0
28	1354.0
29	1333.0
30	1357.5
31	1382.0
32	1665.5
33	1949.0
34	2082.5
35	2216.0
36	2392.0
37	2568.0
38	2837.0
39	3106.0
40	3567.5
41	4029.0
42	4911.0
43	5793.0
44	6803.5
45	7814.0
46	11193.0
47	14572.0
48	13991.0
49	13410.0
50	13026.0
51	12642.0
52	10444.0
53	8246.0
54	7018.5
55	5791.0
56	5036.0
57	4281.0
58	3651.5
59	3022.0
60	2961.5
61	2901.0
62	2528.0
63	2155.0
64	1868.5
65	1582.0
66	1311.5
67	1041.0
68	820.5
69	600.0
70	492.0
71	384.0
72	293.5
73	203.0
74	178.5
75	100.0
76	46.0
77	47.5
78	49.0
79	32.0
80	15.0
81	8.5
82	2.0
83	14.5
84	27.0
85	13.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	156329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.622910656372134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.63067998710925	40.50751939819228
2	7.831131163390268	7.772070441184938
3	2.67869803416049	3.98774379673637
4	1.4308733483725427	2.8401640130749892
5	0.9384466645182082	2.328422749457874
6	0.8005156300354496	2.383434935296714
7	0.6432484692233322	2.234390292268229
8	0.6071543667418627	2.4103013516366127
9	0.5078955849178214	2.268293150982863
>10	2.8565903963905894	20.528500790000574
>50	0.04640670319046084	1.5435395863851236
>100	0.020625201417982596	2.000268664163399
>500	0.0025781501772478245	0.9128184789770292
>1k	0.003867225265871737	2.274689916778077
>5k	0.0012890750886239122	6.007842434864933
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9392	6.007842434864933	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	1317	0.8424540552296758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	1176	0.7522596575171593	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	1063	0.6799762040312418	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	893	0.5712311855126049	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	534	0.3415872934644244	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	385	0.24627548311573672	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTACTCCT	305	0.19510135675402518	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	265	0.1695142935731694	No Hit
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	217	0.13880981775614248	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTA	207	0.13241305196092854	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	191	0.12217822668858624	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	189	0.12089887352954345	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	186	0.11897984379097928	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	173	0.11066404825720115	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	172	0.11002437167767977	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	164	0.10490695904150861	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	163	0.10426728246198723	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2539516020699934	0.0
2	0.0	0.0	0.0	1.469337103160642	0.0
3	0.0	0.0	0.0	2.0060257533790917	0.0
4	0.0	0.0	0.0	2.701993871898368	0.0
5	0.0	0.0	0.0	4.842351706976952	0.0
6	0.0	0.0	0.0	5.589493951857941	0.0
7	0.0	0.0	0.0	6.545810438242425	0.0
8	0.0	0.0	0.0	7.871859987590274	0.0
9	0.0	0.0	0.0	8.306200385085301	0.0
10	0.0	0.0	0.0	10.00326235055556	0.0
11	0.0	0.0	0.0	11.413109531820712	0.0
12	0.0	0.0	0.0	13.344293125395799	0.0
13	0.0	0.0	0.0	13.864350184546693	0.0
14	0.0	0.0	0.0	14.077362485527317	0.0
15	0.0	0.0	0.0	14.540488329100807	0.0
16	0.0	0.0	0.0	15.297865399254137	0.0
17	0.0	0.0	0.0	16.072513737054546	0.0
18	0.0	0.0	0.0	17.01539701526908	0.0
19	0.0	0.0	0.0	17.757421847513896	0.0
20	0.0	0.0	0.0	18.324814973549373	0.0
21	0.0	0.0	0.0	18.96193284675268	0.0
22	0.0	0.0	0.0	19.59393330731982	0.0
23	0.0	0.0	0.0	20.25471921396542	0.0
24	0.0	0.0	0.0	20.766460477582534	0.0
25	0.0	0.0	0.0	21.179691547953354	0.0
26	0.0	0.0	0.0	21.56349749566619	0.0
27	0.0	0.0	0.0	21.98632371472983	0.0
28	0.0	0.0	0.0	22.40659122747539	0.0
29	0.0	0.0	0.0	22.83517453575472	0.0
30	0.0	0.0	0.0	23.29702102616917	0.0
31	0.0	0.0	0.0	23.735199483141322	0.0
32	0.0	0.0	0.0	24.1292402561265	0.0
33	6.39676579521394E-4	0.0	0.0	24.544390356235887	0.0
34	6.39676579521394E-4	0.0	0.0	24.919240831835424	0.0
35	6.39676579521394E-4	0.0	0.0	25.32095772377486	0.0
36	6.39676579521394E-4	0.0	0.0	25.723314292293818	0.0
37	6.39676579521394E-4	0.0	0.0	26.066820615496805	0.0
38	6.39676579521394E-4	0.0	0.0	26.471096213754326	0.0
39	6.39676579521394E-4	0.0	0.0	26.828675421706784	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAG	20	7.014574E-4	45.000004	1
GATACCA	20	7.014574E-4	45.000004	9
CCGGGAT	20	7.014574E-4	45.000004	5
AACCCGC	20	7.014574E-4	45.000004	21
TCCGAAC	20	7.014574E-4	45.000004	17
TGGGTGC	35	1.2029341E-7	45.000004	6
GCTATTA	20	7.014574E-4	45.000004	9
CGTTCTT	20	7.014574E-4	45.000004	1
TGCGGGA	35	1.2029341E-7	45.000004	4
ACTTAGG	20	7.014574E-4	45.000004	2
GCGCAGG	20	7.014574E-4	45.000004	2
GTAATGG	20	7.014574E-4	45.000004	2
TAGCACG	20	7.014574E-4	45.000004	1
CTTATTA	20	7.014574E-4	45.000004	19
GCGGGCA	20	7.014574E-4	45.000004	5
CGGGATT	40	6.7502697E-9	45.000004	6
GCGATTG	30	2.1534397E-6	45.000004	9
GTTCCGA	20	7.014574E-4	45.000004	15
ATGAACA	20	7.014574E-4	45.000004	34
GATTGGG	40	6.7502697E-9	45.000004	3
>>END_MODULE