##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543149
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44453731848903	34.0	31.0	34.0	31.0	34.0
2	32.64348456869109	34.0	31.0	34.0	31.0	34.0
3	32.505726789518164	34.0	31.0	34.0	31.0	34.0
4	36.0985917308142	37.0	35.0	37.0	35.0	37.0
5	36.13077626949511	37.0	35.0	37.0	35.0	37.0
6	36.14512039974298	37.0	37.0	37.0	35.0	37.0
7	36.288593001183834	37.0	37.0	37.0	35.0	37.0
8	36.05878497428883	37.0	37.0	37.0	35.0	37.0
9	37.89426290023548	39.0	38.0	39.0	35.0	39.0
10	37.71281913434435	39.0	38.0	39.0	35.0	39.0
11	37.68529814102576	39.0	38.0	39.0	35.0	39.0
12	37.63937519907061	39.0	37.0	39.0	35.0	39.0
13	37.637392317761794	39.0	38.0	39.0	35.0	39.0
14	39.03968892513841	41.0	39.0	41.0	35.0	41.0
15	39.15585778488039	41.0	39.0	41.0	35.0	41.0
16	39.20779196868631	41.0	39.0	41.0	35.0	41.0
17	39.18117312192419	41.0	39.0	41.0	35.0	41.0
18	38.51078249246523	39.0	39.0	41.0	35.0	41.0
19	37.833347755404134	37.0	37.0	41.0	35.0	41.0
20	37.01990982216666	37.0	35.0	41.0	34.0	41.0
21	36.97567886528374	36.0	35.0	40.0	34.0	41.0
22	36.96640700802174	36.0	35.0	40.0	34.0	41.0
23	36.96071059690803	36.0	35.0	40.0	34.0	41.0
24	36.81085300718588	36.0	35.0	40.0	34.0	41.0
25	36.72805620557158	36.0	35.0	40.0	34.0	41.0
26	36.57968623710989	36.0	35.0	40.0	33.0	41.0
27	36.51092978169894	35.0	35.0	40.0	33.0	41.0
28	36.371588643263635	36.0	35.0	40.0	33.0	41.0
29	36.10254460562387	35.0	35.0	40.0	33.0	41.0
30	35.59248014817297	35.0	35.0	40.0	31.0	41.0
31	34.9333332105923	35.0	35.0	40.0	25.0	41.0
32	33.869313945160535	35.0	34.0	40.0	18.0	41.0
33	32.71771834248061	35.0	33.0	40.0	12.0	41.0
34	31.74583217496488	35.0	30.0	40.0	8.0	41.0
35	31.166987327602556	35.0	25.0	40.0	7.0	41.0
36	30.821949409830452	35.0	23.0	40.0	7.0	41.0
37	30.654704326068906	35.0	23.0	40.0	7.0	41.0
38	30.503743908209348	35.0	23.0	40.0	7.0	41.0
39	30.344934815308505	35.0	22.0	40.0	7.0	41.0
40	30.177078481227067	35.0	21.0	40.0	7.0	41.0
41	30.014592680829754	35.0	21.0	40.0	7.0	41.0
42	29.90979639104555	35.0	20.0	40.0	7.0	41.0
43	29.79121382898615	35.0	20.0	40.0	7.0	41.0
44	29.681019388786503	35.0	18.0	40.0	7.0	41.0
45	29.618487744615198	35.0	20.0	40.0	7.0	41.0
46	29.58056260805046	35.0	20.0	40.0	7.0	41.0
47	29.596177107939074	35.0	20.0	40.0	7.0	41.0
48	29.599273864077812	35.0	20.0	40.0	7.0	41.0
49	29.615586146711124	35.0	20.0	40.0	7.0	41.0
50	29.527043223866748	35.0	20.0	40.0	7.0	41.0
51	28.94310216901808	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	13.0
10	8.0
11	12.0
12	11.0
13	13.0
14	16.0
15	30.0
16	54.0
17	114.0
18	277.0
19	476.0
20	792.0
21	1368.0
22	2277.0
23	3947.0
24	7780.0
25	16277.0
26	28355.0
27	35298.0
28	31245.0
29	23523.0
30	17561.0
31	14354.0
32	13235.0
33	15231.0
34	24460.0
35	35756.0
36	39412.0
37	37845.0
38	67246.0
39	126029.0
40	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.93183638375473	12.99551320171813	14.375245098490469	45.697405316036665
2	57.83900918532484	14.84436130785475	15.223815196198466	12.092814310621947
3	20.35574032171651	15.399457607396865	53.27304294033497	10.971759130551654
4	17.976650974226224	16.016599496639046	52.80190150400719	13.204848025127545
5	16.136456110570027	19.18552735989572	51.65009969640006	13.027916833134187
6	16.507440867975454	22.111796210616237	51.59099989137419	9.789763030034116
7	50.81552207589446	3.6431991958007837	41.7496856295418	3.791593098762955
8	50.83025099926539	3.622394591539338	42.117908713815176	3.4294456953800894
9	47.347597068207804	4.517913132492189	43.421050209058656	4.713439590241352
10	26.446334247140285	15.833040289128766	47.91005782943539	9.81056763429556
11	18.61846381011472	16.17916998834574	51.990706049352944	13.2116601521866
12	16.502838079422038	14.182112090789085	55.18099085149747	14.134058978291408
13	16.027093854540837	14.84822765023962	55.70626108121344	13.4184174140061
14	13.11665859644407	17.26045707531451	55.173626389812	14.449257938429419
15	12.797225070836916	15.848873881752521	57.59045860344031	13.763442443970256
16	14.560645421422114	16.17751298446651	55.45678994161823	13.805051652493146
17	14.804040880126815	15.573443014716034	54.320453503550596	15.302062601606558
18	15.022581280643067	15.85568600881158	55.25831769919488	13.863415011350478
19	14.901251774374987	16.68492439459522	54.14665220777356	14.267171623256234
20	16.377274007684818	16.480008248197088	54.10633178004562	13.036385964072474
21	15.768417137838789	16.93200208414266	54.4544867062261	12.845094071792454
22	15.766576022417421	14.71990190537035	54.01501245514583	15.498509617066404
23	14.695599181808305	16.402865512041814	54.545621919583766	14.355913386566115
24	14.506700739576065	16.09521512513141	54.4614829448273	14.936601190465232
25	14.057100353678273	17.521159018980057	53.38222108482202	15.039519542519638
26	14.172722402140112	17.342386711565336	53.584743781172385	14.900147105122166
27	14.052497565124856	16.97526829654478	54.37053184301177	14.601702295318598
28	12.627658340529027	17.119979968664214	54.98270272061626	15.2696589701905
29	13.760864882380341	17.04302134405108	53.93676504973774	15.259348723830845
30	14.773110141047852	16.869772382900454	53.67109209443449	14.686025381617199
31	15.340357802370988	17.558165438949533	51.245054303699355	15.856422454980127
32	15.299300928474507	19.366509005816084	48.8440556826948	16.490134383014606
33	16.37248710758926	19.446965749729817	46.4307215883671	17.749825554313826
34	15.365581083643715	21.86655963649017	45.326604670173374	17.441254609692734
35	16.630979712749173	22.87843667207341	41.94097752182182	18.549606093355596
36	17.897114788023178	23.89068193074092	40.63949303045757	17.572710250778332
37	16.866642486684132	24.764475309721643	40.72731423605677	17.64156796753745
38	17.79953567069073	24.752139836398484	38.445619894356795	19.002704598553986
39	18.107370169143273	23.950886405019617	38.550195250290436	19.391548175546674
40	18.522725808203642	23.581742763035557	39.82590412575554	18.069627303005255
41	16.72156259148042	24.982831598695753	38.14791153072177	20.14769427910205
42	17.530180484544758	24.54280501298907	38.27255504474831	19.654459457717863
43	17.947193127484354	24.35004022837196	38.15951055787639	19.543256086267306
44	17.411428539866595	24.306221681343427	38.74995627350874	19.532393505281238
45	17.55945421974449	23.302261442072066	39.03753850232625	20.100745835857197
46	18.094114138109433	23.163993673927415	39.782637913353426	18.95925427460973
47	16.197765254101544	23.378483620516654	41.86917402038851	18.55457710499329
48	16.606492877644992	22.142174615068793	41.23012285763207	20.021209649654146
49	17.09033801038021	21.443839535744335	42.25875404354974	19.20706841032571
50	16.14860747235105	21.243526177899618	42.65974898232345	19.94811736742588
51	16.336033022246195	21.353993103181633	40.89853797024389	21.411435904328275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	310.5
2	491.0
3	31963.0
4	63435.0
5	41851.5
6	20268.0
7	19705.5
8	19143.0
9	18813.5
10	18484.0
11	17705.5
12	16927.0
13	15796.0
14	14665.0
15	13409.0
16	12153.0
17	10976.0
18	9799.0
19	8795.0
20	7791.0
21	7104.5
22	6418.0
23	5873.0
24	5328.0
25	5082.0
26	4800.5
27	4765.0
28	4953.0
29	5141.0
30	6016.0
31	6891.0
32	7056.0
33	7221.0
34	7701.5
35	8182.0
36	8911.0
37	9640.0
38	11490.5
39	13341.0
40	14339.0
41	15337.0
42	17024.0
43	18711.0
44	20925.5
45	23140.0
46	26921.5
47	30703.0
48	32476.0
49	34249.0
50	33929.0
51	33609.0
52	29912.5
53	26216.0
54	23068.0
55	19920.0
56	18599.0
57	17278.0
58	16219.5
59	15161.0
60	14417.5
61	13674.0
62	12829.0
63	11984.0
64	10494.5
65	9005.0
66	8086.0
67	7167.0
68	5932.5
69	4698.0
70	3918.5
71	3139.0
72	2451.0
73	1763.0
74	1358.5
75	867.0
76	780.0
77	548.5
78	317.0
79	258.0
80	199.0
81	137.5
82	76.0
83	45.0
84	14.0
85	8.5
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	543149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.97998511199395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.80635955787145	34.73056130751744
2	7.645251336576808	6.571855772470461
3	2.637317609912319	3.4005561482889273
4	1.3234568761490741	2.2752862733301287
5	0.9220837490719583	1.9815572903562169
6	0.6808343418336812	1.755734992544749
7	0.5781684464685257	1.7394769855009335
8	0.5319055187276164	1.8289033020720296
9	0.4783472559812872	1.8503422146394992
>10	4.271715434380638	30.85800465732453
>50	0.0926656494841011	2.781639433474168
>100	0.02758419333411158	2.26517627809534
>500	0.0038790271876094403	1.188346894339353
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.310030208454934E-4	6.772558450046258
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36560	6.7311179805173165	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	997	0.18355920751027804	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	776	0.14287055669806994	TruSeq Adapter, Index 22 (95% over 23bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	754	0.13882010277106283	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	732	0.13476964884405568	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	687	0.12648462944790473	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	661	0.12169772935235082	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	639	0.11764727542534369	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	591	0.10880992140278266	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	578	0.10641647135500572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.841115421366881E-4	0.0	0.0	0.09316044032116418	0.0
2	1.841115421366881E-4	0.0	0.0	0.5392627069183594	0.0
3	1.841115421366881E-4	0.0	0.0	0.779160046322464	0.0
4	1.841115421366881E-4	0.0	0.0	1.0779730792103088	0.0
5	1.841115421366881E-4	0.0	0.0	1.9747804009581165	0.0
6	1.841115421366881E-4	0.0	0.0	2.383508024501564	0.0
7	1.841115421366881E-4	0.0	0.0	2.8343971911943133	0.0
8	1.841115421366881E-4	0.0	0.0	3.5677134635247416	0.0
9	1.841115421366881E-4	0.0	0.0	3.8098201414344866	0.0
10	3.682230842733762E-4	0.0	0.0	4.507602886132535	0.0
11	3.682230842733762E-4	0.0	0.0	5.342548729722415	0.0
12	3.682230842733762E-4	0.0	0.0	6.172339450132468	0.0
13	5.523346264100643E-4	0.0	0.0	6.522519603276449	0.0
14	5.523346264100643E-4	0.0	0.0	6.639246320991109	0.0
15	5.523346264100643E-4	0.0	0.0	6.920752868918106	0.0
16	5.523346264100643E-4	0.0	0.0	7.367775693225984	0.0
17	5.523346264100643E-4	0.0	0.0	7.900226273085286	0.0
18	5.523346264100643E-4	0.0	0.0	8.476679511515256	0.0
19	5.523346264100643E-4	0.0	0.0	8.845086707330768	0.0
20	7.364461685467524E-4	0.0	0.0	9.207786445340044	0.0
21	7.364461685467524E-4	0.0	0.0	9.629033653748786	0.0
22	7.364461685467524E-4	0.0	0.0	10.08139571277863	0.0
23	7.364461685467524E-4	0.0	0.0	10.520869963858905	0.0
24	7.364461685467524E-4	0.0	0.0	10.89075005201151	0.0
25	7.364461685467524E-4	0.0	0.0	11.255659128526426	0.0
26	7.364461685467524E-4	0.0	0.0	11.549132926692307	0.0
27	7.364461685467524E-4	0.0	0.0	11.860097321361174	0.0
28	7.364461685467524E-4	0.0	0.0	12.15485990032201	0.0
29	7.364461685467524E-4	0.0	0.0	12.465824294990878	0.0
30	7.364461685467524E-4	0.0	0.0	12.804589532522384	0.0
31	7.364461685467524E-4	0.0	0.0	13.120340827286803	0.0
32	7.364461685467524E-4	0.0	0.0	13.457080837854805	0.0
33	7.364461685467524E-4	0.0	0.0	13.750738747562824	0.0
34	7.364461685467524E-4	0.0	0.0	14.09668433523766	0.0
35	7.364461685467524E-4	0.0	0.0	14.436554242021987	0.0
36	7.364461685467524E-4	0.0	0.0	14.734078494114874	0.0
37	7.364461685467524E-4	0.0	0.0	15.040992434856733	0.0
38	7.364461685467524E-4	0.0	0.0	15.34422414475586	0.0
39	7.364461685467524E-4	0.0	0.0	15.6721268013013	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGAC	20	7.0299953E-4	45.000004	26
ACCGAAC	20	7.0299953E-4	45.000004	11
GCGTTAT	20	7.0299953E-4	45.000004	11
GTTCCGA	50	2.1827873E-11	45.000004	15
GCGATTA	25	3.8880782E-5	45.000004	9
AACTCGT	20	7.0299953E-4	45.000004	44
CAACTCG	25	3.8880782E-5	45.000004	39
AGTACGG	30	2.1633787E-6	45.000004	2
CCCGCAT	50	2.1827873E-11	45.000004	23
CGTTTTT	20790	0.0	44.686146	1
TGCATTA	275	0.0	43.363636	15
CTCAACT	380	0.0	42.039474	2
CGGGTAT	55	6.002665E-11	40.909092	6
TCCGAAC	55	6.002665E-11	40.909092	17
TTCCGAA	55	6.002665E-11	40.909092	16
CCATACG	55	6.002665E-11	40.909092	2
GGCGATA	55	6.002665E-11	40.909092	8
ACTTGAC	375	0.0	40.8	6
CAACTTG	375	0.0	40.8	4
GTTTTTT	22995	0.0	40.567513	2
>>END_MODULE