##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935668.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 613768 Sequences flagged as poor quality 0 Sequence length 51 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.451040132427885 34.0 31.0 34.0 31.0 34.0 2 32.651213487832536 34.0 31.0 34.0 31.0 34.0 3 32.50199749742574 34.0 31.0 34.0 31.0 34.0 4 36.094835507879196 37.0 35.0 37.0 35.0 37.0 5 36.13283683737178 37.0 35.0 37.0 35.0 37.0 6 36.151663169145344 37.0 37.0 37.0 35.0 37.0 7 36.309959137654616 37.0 37.0 37.0 35.0 37.0 8 36.12647449850758 37.0 37.0 37.0 35.0 37.0 9 37.94414013112446 39.0 39.0 39.0 35.0 39.0 10 37.7587459756781 39.0 38.0 39.0 35.0 39.0 11 37.70335533947681 39.0 38.0 39.0 35.0 39.0 12 37.710742495535776 39.0 38.0 39.0 35.0 39.0 13 37.73135614759975 39.0 38.0 39.0 35.0 39.0 14 39.17750029327042 41.0 39.0 41.0 35.0 41.0 15 39.2750795088698 41.0 39.0 41.0 35.0 41.0 16 39.30355280822721 41.0 39.0 41.0 35.0 41.0 17 39.27406120879551 41.0 39.0 41.0 35.0 41.0 18 38.608821248419595 39.0 39.0 41.0 35.0 41.0 19 37.92790109617967 37.0 37.0 41.0 35.0 41.0 20 37.12911230302004 37.0 35.0 41.0 34.0 41.0 21 37.085540464801035 37.0 35.0 41.0 34.0 41.0 22 37.08296131437286 37.0 35.0 41.0 34.0 41.0 23 37.08117888192281 37.0 35.0 41.0 34.0 41.0 24 36.929388628928194 37.0 35.0 41.0 34.0 41.0 25 36.83849434965655 36.0 35.0 41.0 34.0 41.0 26 36.70328528043169 36.0 35.0 40.0 34.0 41.0 27 36.6270545222299 36.0 35.0 40.0 33.0 41.0 28 36.4899766687087 36.0 35.0 40.0 33.0 41.0 29 36.25223211376286 36.0 35.0 40.0 33.0 41.0 30 35.73100422309407 36.0 35.0 40.0 31.0 41.0 31 35.05633073082989 36.0 35.0 40.0 25.0 41.0 32 34.01120455937748 36.0 34.0 40.0 18.0 41.0 33 32.82881968431069 36.0 33.0 40.0 12.0 41.0 34 31.831258716648637 36.0 30.0 40.0 8.0 41.0 35 31.218556522985885 36.0 24.0 40.0 7.0 41.0 36 30.860215912201355 36.0 23.0 40.0 7.0 41.0 37 30.70563144380287 36.0 22.0 40.0 7.0 41.0 38 30.544653680217934 36.0 22.0 40.0 7.0 41.0 39 30.419896442955643 35.0 21.0 40.0 7.0 41.0 40 30.286521943144642 35.0 21.0 40.0 7.0 41.0 41 30.124789822864667 35.0 20.0 40.0 7.0 41.0 42 30.02122300282843 35.0 20.0 40.0 7.0 41.0 43 29.904623571121334 35.0 18.0 40.0 7.0 41.0 44 29.788165886784583 35.0 18.0 40.0 7.0 41.0 45 29.717442095384577 35.0 20.0 40.0 7.0 41.0 46 29.686694320981218 35.0 20.0 40.0 7.0 41.0 47 29.701147664915734 35.0 20.0 40.0 7.0 41.0 48 29.669482605805452 35.0 20.0 40.0 7.0 41.0 49 29.649910063737437 35.0 20.0 40.0 7.0 41.0 50 29.55052397648623 35.0 20.0 40.0 7.0 41.0 51 28.95350849180798 35.0 20.0 39.0 7.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 8.0 10 10.0 11 6.0 12 6.0 13 7.0 14 9.0 15 24.0 16 69.0 17 131.0 18 296.0 19 552.0 20 1061.0 21 1522.0 22 2450.0 23 4523.0 24 9112.0 25 19660.0 26 33513.0 27 39504.0 28 35168.0 29 25762.0 30 19533.0 31 15989.0 32 14915.0 33 16092.0 34 24237.0 35 35195.0 36 39054.0 37 38857.0 38 75700.0 39 160621.0 40 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.211969343465285 13.675036821730687 15.781533087420655 45.33146074738338 2 58.14574888231384 15.08843732485239 15.865603941554463 10.90020985127931 3 20.25130016553486 15.438243766374265 53.39216120749208 10.918294860598794 4 18.147573676046974 16.466156593370783 53.52820609741792 11.858063633164322 5 16.305835429673753 19.014024843263254 52.27659311010024 12.403546616962762 6 16.81433375477379 21.513340545613328 51.8215351728992 9.850790526713677 7 52.33866868262926 3.4557031321281 41.17972914847304 3.0258990367695935 8 53.43778105082051 2.9206475410904447 40.842305235854596 2.79926617223446 9 49.49769293935168 4.815826175362678 42.05432671628368 3.632154169001968 10 24.30071297298002 20.97062733801697 46.39407724091188 8.334582448091135 11 16.595358506797357 16.492388003284628 54.039800054743814 12.872453435174203 12 16.25060283364398 14.737816243271073 55.693682303411066 13.317898619673885 13 15.208678197625161 15.576732576478408 55.93774846521813 13.276840760678303 14 13.161976512297807 17.62734453409106 55.091989155511534 14.118689798099608 15 12.506842976499263 17.29399382176979 57.10708280653276 13.092080395198186 16 13.981993196126224 17.281611292866362 55.726104977776615 13.010290533230798 17 14.256363968144314 16.697840226274423 54.307653706286416 14.738142099294848 18 14.396156202343558 16.9073656495614 55.21907300478357 13.47740514331148 19 15.014305079443698 17.628647958186157 54.05821092008707 13.298836042283076 20 15.715221386582554 17.230614825145658 54.29217554515713 12.761988243114661 21 15.665528342956947 16.726678484378464 54.89598675721119 12.711806415453397 22 14.676229454777701 15.958309980318298 54.40638808149008 14.95907248341393 23 13.88472517302955 16.845778861068027 54.95105642522907 14.31843954067335 24 14.149483192346294 16.544850823112316 55.08335397088151 14.222312013659884 25 14.172944826058053 17.589708163345108 53.77862645168858 14.458720558908253 26 13.52856453904407 18.042973892415375 53.63459808918027 14.793863479360281 27 13.95869449042635 17.664329192789456 54.35979067009033 14.017185646693864 28 12.778932756350933 18.041018756272727 54.68955696615008 14.490491521226263 29 14.431674508934972 16.655316015171856 53.849174280835754 15.063835195057415 30 14.696432528251718 17.448775433062654 52.44196504216577 15.412826996519858 31 14.852517563639681 19.101191329622917 50.69309576256826 15.353195344169132 32 15.811348913596015 20.140834973475318 48.8531497243258 15.19466638860286 33 15.591721953572032 20.882809139609755 46.54641493202643 16.97905397479178 34 15.57363694425255 22.65628054900223 44.714452366366444 17.055630140378774 35 16.816940602963985 23.166734010244912 41.979542758827435 18.03678262796366 36 17.036241706964194 24.848965732980542 40.550989950600226 17.56380260945504 37 17.167235828521527 25.424590398978115 40.87684597437468 16.531327798125677 38 17.07485564578147 25.733664837528185 39.58401220004953 17.607467316640815 39 17.752962031256107 24.07554646055187 40.27857431472478 17.89291719346724 40 17.759642079743486 24.379895986757212 40.50781402745011 17.35264790604919 41 16.61083666792664 25.41318543814601 39.28031438589174 18.695663508035608 42 17.295460173876776 25.52251013412234 38.947615385618015 18.234414306382867 43 17.705061195761264 24.970510029848413 38.94174997719008 18.382678797200246 44 17.771372896599367 24.762125102644646 38.113749820779184 19.352752179976797 45 17.653575944004903 23.959704644100054 38.47821978337092 19.908499628524133 46 17.81063854746419 24.222344599262264 39.41521877973436 18.55179807353919 47 16.441065679540152 23.999622007012423 41.207101054470094 18.352211258977334 48 16.2312144002294 23.378214569674537 41.09435487024413 19.29621615985193 49 16.795922889430535 22.750452939873046 41.785658424681635 18.66796574601478 50 16.13801957742991 22.15136664016371 42.08854159878 19.622072183626386 51 15.982423326077605 22.29018130629163 40.721412651034264 21.0059827165965 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 102.0 1 285.5 2 469.0 3 34421.5 4 68374.0 5 45815.0 6 23256.0 7 22602.0 8 21948.0 9 21209.0 10 20470.0 11 19723.5 12 18977.0 13 17873.5 14 16770.0 15 15442.5 16 14115.0 17 12914.0 18 11713.0 19 10760.5 20 9808.0 21 8877.5 22 7947.0 23 7284.0 24 6621.0 25 6502.0 26 6478.5 27 6574.0 28 6813.5 29 7053.0 30 7227.5 31 7402.0 32 8657.0 33 9912.0 34 11406.5 35 12901.0 36 13399.0 37 13897.0 38 15134.0 39 16371.0 40 17688.0 41 19005.0 42 20608.0 43 22211.0 44 24841.0 45 27471.0 46 30575.5 47 33680.0 48 36648.5 49 39617.0 50 39334.5 51 39052.0 52 34462.5 53 29873.0 54 26284.5 55 22696.0 56 20886.0 57 19076.0 58 17466.5 59 15857.0 60 14985.0 61 14113.0 62 12381.5 63 10650.0 64 8880.0 65 7110.0 66 5936.5 67 4763.0 68 3993.0 69 3223.0 70 2539.0 71 1855.0 72 1381.5 73 908.0 74 853.0 75 563.0 76 328.0 77 263.0 78 198.0 79 173.0 80 148.0 81 91.5 82 35.0 83 28.0 84 21.0 85 11.5 86 2.0 87 8.0 88 14.0 89 7.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 613768.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.10767080321818 #Duplication Level Percentage of deduplicated Percentage of total 1 80.12554222366245 35.34151039330662 2 7.726899075876126 6.8163104153687 3 2.7223106483902115 3.6022434570987265 4 1.5167884716854214 2.676080263488679 5 1.044446348134034 2.3034047847559695 6 0.8238453903616515 2.18027407624923 7 0.6938757910745808 2.1423671479728146 8 0.6262662435008477 2.209851624280277 9 0.5349268244366207 2.1234938648455213 >10 4.070842834861766 28.488313785305273 >50 0.08076086039632867 2.5210995260300737 >100 0.030517928720490996 2.274373867209877 >500 0.002605189037115085 0.8534442248736306 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 3.7216986244501216E-4 6.467232569214546 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39397 6.418874884321111 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 911 0.14842741882926447 No Hit GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC 868 0.14142151431811367 No Hit CCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC 777 0.12659506523637595 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 763 0.12431407306995478 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 693 0.11290911223784883 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.258560237744555E-4 0.0 0.0 0.10215586345329179 0.0 2 3.258560237744555E-4 0.0 0.0 0.4861771874714876 0.0 3 3.258560237744555E-4 0.0 0.0 0.7181866763988999 0.0 4 3.258560237744555E-4 0.0 0.0 0.9966306487141722 0.0 5 3.258560237744555E-4 0.0 0.0 1.7345316145514267 0.0 6 3.258560237744555E-4 0.0 0.0 2.180791439110543 0.0 7 3.258560237744555E-4 0.0 0.0 2.5872968287691767 0.0 8 3.258560237744555E-4 0.0 0.0 3.288050207896143 0.0 9 3.258560237744555E-4 0.0 0.0 3.5560667874506326 0.0 10 3.258560237744555E-4 0.0 0.0 4.100572203177748 0.0 11 3.258560237744555E-4 0.0 0.0 4.923684519232022 0.0 12 3.258560237744555E-4 0.0 0.0 5.627207674561072 0.0 13 3.258560237744555E-4 0.0 0.0 5.8944096140561255 0.0 14 3.258560237744555E-4 0.0 0.0 6.000801605818485 0.0 15 3.258560237744555E-4 0.0 0.0 6.1907756676789925 0.0 16 3.258560237744555E-4 0.0 0.0 6.599073265468386 0.0 17 3.258560237744555E-4 0.0 0.0 7.112948214960702 0.0 18 3.258560237744555E-4 0.0 0.0 7.6757015680191865 0.0 19 3.258560237744555E-4 0.0 0.0 8.033491482123539 0.0 20 3.258560237744555E-4 0.0 0.0 8.387045267918822 0.0 21 3.258560237744555E-4 0.0 0.0 8.806421970516547 0.0 22 3.258560237744555E-4 0.0 0.0 9.284941541429335 0.0 23 3.258560237744555E-4 0.0 0.0 9.745213175010754 0.0 24 3.258560237744555E-4 0.0 0.0 10.097789392734715 0.0 25 3.258560237744555E-4 0.0 0.0 10.41598779995047 0.0 26 3.258560237744555E-4 0.0 0.0 10.704696237014637 0.0 27 3.258560237744555E-4 0.0 0.0 11.010837971350739 0.0 28 3.258560237744555E-4 0.0 0.0 11.314047001472868 0.0 29 3.258560237744555E-4 0.0 0.0 11.634689328866934 0.0 30 4.887840356616833E-4 0.0 0.0 12.010564252290768 0.0 31 4.887840356616833E-4 0.0 0.0 12.341145188409953 0.0 32 4.887840356616833E-4 0.0 0.0 12.654944539304754 0.0 33 4.887840356616833E-4 0.0 0.0 12.955220865212913 0.0 34 6.51712047548911E-4 0.0 0.0 13.264132375751098 0.0 35 6.51712047548911E-4 0.0 0.0 13.58835911940668 0.0 36 8.146400594361388E-4 0.0 0.0 13.910956582943392 0.0 37 9.775680713233665E-4 0.0 0.0 14.236323822682186 0.0 38 9.775680713233665E-4 0.0 0.0 14.5652754786825 0.0 39 9.775680713233665E-4 0.0 0.0 14.927464449107806 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTAG 25 3.8886734E-5 45.0 1 CGGTTTA 20 7.030714E-4 45.0 14 GTAACGG 25 3.8886734E-5 45.0 2 ACGGTCT 25 3.8886734E-5 45.0 30 CGTTTTT 22255 0.0 44.666367 1 GTTTTTT 24675 0.0 40.395138 2 GTCGATG 45 1.925764E-8 40.0 1 GTATGCG 45 1.925764E-8 40.0 1 CGCCCAA 85 0.0 39.705883 37 TAATGCG 40 3.4547156E-7 39.375 1 GTTAGCG 35 6.24353E-6 38.57143 1 TAACGGG 105 0.0 38.57143 3 GACGGTC 35 6.24353E-6 38.57143 29 TCGTAAG 35 6.24353E-6 38.57143 1 CGGTCTA 35 6.24353E-6 38.57143 31 TGCGGGC 165 0.0 38.181816 4 TCCGCCC 90 0.0 37.5 35 CTACGAA 90 0.0 37.5 11 AGCGTTG 30 1.1393022E-4 37.499996 1 ATCTCCG 30 1.1393022E-4 37.499996 29 >>END_MODULE