##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935666.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	245306
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33893585970176	33.0	31.0	34.0	30.0	34.0
2	32.55105867773312	34.0	31.0	34.0	31.0	34.0
3	32.38381857761327	34.0	31.0	34.0	30.0	34.0
4	36.01282479841504	37.0	35.0	37.0	35.0	37.0
5	36.06339836775293	37.0	35.0	37.0	35.0	37.0
6	36.075305944412285	37.0	36.0	37.0	35.0	37.0
7	36.23780502719053	37.0	37.0	37.0	35.0	37.0
8	36.01225408265595	37.0	37.0	37.0	35.0	37.0
9	37.84653045583883	39.0	38.0	39.0	35.0	39.0
10	37.67794102060284	39.0	38.0	39.0	35.0	39.0
11	37.62208833049334	39.0	37.0	39.0	35.0	39.0
12	37.54447098725673	39.0	37.0	39.0	35.0	39.0
13	37.50802263295639	39.0	37.0	39.0	35.0	39.0
14	38.89115635165874	41.0	38.0	41.0	35.0	41.0
15	39.024989197165986	41.0	38.0	41.0	35.0	41.0
16	39.07293339747091	41.0	39.0	41.0	35.0	41.0
17	39.04272622765036	41.0	38.0	41.0	35.0	41.0
18	38.352914319258396	39.0	38.0	40.0	35.0	41.0
19	37.65447644982186	37.0	37.0	40.0	35.0	41.0
20	36.81405672914645	35.0	35.0	40.0	34.0	41.0
21	36.78118757796385	35.0	35.0	40.0	34.0	41.0
22	36.76745778741653	35.0	35.0	40.0	34.0	41.0
23	36.79716761921845	35.0	35.0	40.0	34.0	41.0
24	36.61813000904992	35.0	35.0	40.0	34.0	41.0
25	36.540206925228084	35.0	35.0	40.0	33.0	41.0
26	36.394609182001254	35.0	35.0	40.0	33.0	41.0
27	36.29841096426504	35.0	35.0	40.0	33.0	41.0
28	36.1663717968578	35.0	35.0	40.0	33.0	41.0
29	35.90366317986515	35.0	35.0	40.0	33.0	41.0
30	35.34391739297041	35.0	35.0	40.0	31.0	41.0
31	34.63194948350224	35.0	35.0	40.0	23.0	41.0
32	33.548947029424475	35.0	34.0	40.0	17.0	41.0
33	32.38509861153009	35.0	33.0	40.0	10.0	41.0
34	31.434066023660243	35.0	29.0	40.0	8.0	41.0
35	30.851935949385666	35.0	24.0	40.0	7.0	41.0
36	30.538877972817623	35.0	23.0	40.0	7.0	41.0
37	30.40069138137673	35.0	23.0	40.0	7.0	41.0
38	30.251277995646255	35.0	22.0	40.0	7.0	41.0
39	30.118284917613103	35.0	21.0	40.0	7.0	41.0
40	29.977424115186746	35.0	21.0	40.0	7.0	41.0
41	29.79943417609027	35.0	20.0	40.0	7.0	41.0
42	29.696766487570628	35.0	20.0	40.0	7.0	41.0
43	29.56870602431249	35.0	18.0	40.0	7.0	41.0
44	29.440559953690492	35.0	18.0	40.0	7.0	41.0
45	29.346803584094967	35.0	20.0	40.0	7.0	41.0
46	29.27243524414405	35.0	20.0	40.0	7.0	41.0
47	29.25100079084898	35.0	20.0	40.0	7.0	41.0
48	29.1765916854867	35.0	20.0	40.0	7.0	41.0
49	29.153261640563215	35.0	18.0	40.0	7.0	41.0
50	29.030113409374415	35.0	18.0	40.0	7.0	41.0
51	28.417258444554964	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	5.0
11	7.0
12	4.0
13	2.0
14	6.0
15	13.0
16	26.0
17	68.0
18	118.0
19	253.0
20	430.0
21	704.0
22	1137.0
23	1908.0
24	3882.0
25	8223.0
26	13318.0
27	15753.0
28	13867.0
29	10783.0
30	8303.0
31	6917.0
32	6488.0
33	7263.0
34	11568.0
35	18540.0
36	17786.0
37	15802.0
38	29311.0
39	52769.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.952508295761213	13.175380952769194	14.804366790865286	47.06774396060431
2	58.97409765761946	13.793792243157526	15.362445272435243	11.869664826787767
3	19.445101220516417	15.47658842425379	53.95669082696713	11.12161952826266
4	18.000375041784547	16.194874972483348	54.183346514149676	11.621403471582433
5	15.67878486461807	18.016681206330055	53.691715653102655	12.612818275949223
6	16.070948121937498	20.361915322087516	53.654619128761624	9.912517427213357
7	48.68613079174582	3.6807089920344387	43.84319992173041	3.7899602944893314
8	48.63884291456385	3.491965137420202	43.66464741995711	4.2045445280588325
9	45.21658663057569	4.733271913446878	45.147693085370925	4.902448370606508
10	25.972866542196275	15.481887927731078	49.37751216847529	9.167733361597353
11	18.611040904013763	15.42930054707182	53.50419476082933	12.455463788085087
12	17.854434869102263	14.070589386317497	55.944819939178004	12.130155805402232
13	15.561380479890422	15.28132210382135	56.82168393761261	12.335613478675613
14	11.990330444424515	18.401914343717642	56.260751877247195	13.34700333461065
15	11.250438228172161	16.247462353142605	59.748640473531026	12.753458945154216
16	13.106895061678067	15.52509926377667	58.00836506241185	13.359640612133417
17	12.96177019722306	15.512054332140265	55.759744971586514	15.766430499050166
18	13.914457860794274	16.046488875119238	57.0487472789088	12.990305985177697
19	15.539774811867627	15.925415603368853	54.816433352628955	13.718376232134558
20	15.338801333844259	17.11250438228172	55.01129201894776	12.537402264926257
21	14.836163811729024	15.847146013550423	56.53714136629353	12.779548808427027
22	13.424049962088166	15.777029506004745	54.84130025356085	15.95762027834623
23	12.640131101562947	16.46107310868874	56.41973698156588	14.479058808182433
24	14.215714250772503	15.182262154207399	56.13315613967861	14.468867455341492
25	13.150921705950935	16.24134754143804	55.340268888653355	15.267461863957669
26	12.241853032538952	17.840166975124948	54.72715710174232	15.190822890593788
27	13.309906810269624	16.763144806894246	55.19922056533473	14.727727817501407
28	12.018050924151876	16.277221103438155	56.17229093458782	15.53243703782215
29	13.051046448109707	15.814533684459409	55.32314741588057	15.811272451550309
30	14.0457224853856	16.686098179416728	53.81971904478489	15.448460290412791
31	15.306596658866884	18.303669702330964	51.506281949891154	14.883451688910993
32	16.35141415212021	19.77733932313111	48.01717039126642	15.85407613348226
33	15.463135838503748	21.993347084865434	45.534964493326704	17.00855258330412
34	15.475773116026515	22.18372155593422	45.093475088257115	17.24703023978215
35	14.919325250911106	24.005527789780924	42.0332156571792	19.04193130212877
36	16.85608994480363	25.20076965096655	41.10131835340351	16.841822050826316
37	17.031381213667824	25.20606915444384	41.39442166110898	16.36812797077935
38	17.44392717666914	26.65242594963026	39.05367174060154	16.84997513309907
39	17.498145173782948	26.158349163901413	39.67126772276259	16.672237939553046
40	17.367288203305257	24.882391788215536	40.57789047149275	17.17242953698646
41	15.391388714503517	26.00017936781	38.797257303123445	19.811174614563036
42	17.110058457599894	25.947184333037104	38.17028527634873	18.772471933014277
43	18.22988430776255	24.763356787033338	38.60810579439557	18.398653110808542
44	17.44759606369188	24.87790759296552	38.1560173823714	19.518478960971194
45	17.535241698123976	23.859179962985007	38.0801937172348	20.52538462165622
46	17.819376615329425	24.294146902236392	39.76258224421743	18.12389423821676
47	16.44028274889322	24.028764074258273	41.202416573585644	18.32853660326286
48	16.088477248823917	23.36510317725616	41.191817566631066	19.354602007288857
49	16.695066569916758	22.23590128247984	42.472666791680595	18.596365355922806
50	16.3946254881658	21.958288831092595	41.59498748501871	20.05209819572289
51	15.201421897548368	22.69818104734495	40.209778806877935	21.890618248228744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	141.5
2	202.0
3	15431.0
4	30660.0
5	20038.5
6	9417.0
7	9100.0
8	8783.0
9	8538.0
10	8293.0
11	8003.5
12	7714.0
13	7282.0
14	6850.0
15	6280.0
16	5710.0
17	5236.0
18	4762.0
19	4234.5
20	3707.0
21	3432.0
22	3157.0
23	2913.0
24	2669.0
25	2576.5
26	2450.5
27	2417.0
28	2210.0
29	2003.0
30	2326.5
31	2650.0
32	2888.0
33	3126.0
34	3224.0
35	3322.0
36	3746.5
37	4171.0
38	4782.5
39	5394.0
40	6003.0
41	6612.0
42	7895.5
43	9179.0
44	10269.5
45	11360.0
46	12762.5
47	14165.0
48	15767.0
49	17369.0
50	17065.0
51	16761.0
52	14295.5
53	11830.0
54	10639.0
55	9448.0
56	8295.5
57	7143.0
58	6835.5
59	6528.0
60	5907.0
61	5286.0
62	4579.0
63	3872.0
64	3434.0
65	2996.0
66	2421.0
67	1846.0
68	1492.5
69	1139.0
70	1051.0
71	963.0
72	705.0
73	447.0
74	400.0
75	274.0
76	195.0
77	162.5
78	130.0
79	96.0
80	62.0
81	42.5
82	23.0
83	23.5
84	24.0
85	12.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	245306.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.64701481781397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98613660610845	34.475090748486934
2	9.053069976026302	7.902789587806534
3	2.945912077005168	3.8574080423106514
4	1.5082789900949218	2.633275017202821
5	1.008618913192476	2.2011602324819712
6	0.7351987559937614	1.9253538598138857
7	0.5576258290502114	1.7037091976345267
8	0.5546319338767174	1.9366422589079921
9	0.47383106450349066	1.8613180344171982
>10	4.096892733090535	29.500256067700086
>50	0.05286956770088466	1.6018218873679726
>100	0.023940936317381728	2.1787215885809554
>500	0.0019950780264484775	0.6021526692660795
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	9.975390132242387E-4	7.6203008080223595
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17502	7.134762296886338	No Hit
GCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	705	0.2873961501145508	No Hit
CCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	678	0.276389489046334	No Hit
CTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGCT	481	0.19608162865971482	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	412	0.16795349481871621	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	353	0.14390190211409423	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	345	0.14064066920499296	No Hit
TCTGTCTCTTATACACATCTGACGCCCGGATAATCGTATGCCGTCTTCTGC	292	0.11903500118219694	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	283	0.11536611415945798	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.076541136376607E-4	0.0	0.0	0.20301174859155505	0.0
2	4.076541136376607E-4	0.0	0.0	0.9885612255713272	0.0
3	4.076541136376607E-4	0.0	0.0	1.3998842262317268	0.0
4	4.076541136376607E-4	0.0	0.0	1.821398579733068	0.0
5	4.076541136376607E-4	0.0	0.0	2.951008128623026	0.0
6	4.076541136376607E-4	0.0	0.0	3.490742175079289	0.0
7	4.076541136376607E-4	0.0	0.0	4.074502865808419	0.0
8	4.076541136376607E-4	0.0	0.0	4.998654741424995	0.0
9	4.076541136376607E-4	0.0	0.0	5.310510138357806	0.0
10	4.076541136376607E-4	0.0	0.0	6.004745093882742	0.0
11	4.076541136376607E-4	0.0	0.0	7.155960310795496	0.0
12	4.076541136376607E-4	0.0	0.0	8.043830970298322	0.0
13	4.076541136376607E-4	0.0	0.0	8.404604860867652	0.0
14	4.076541136376607E-4	0.0	0.0	8.532200598436239	0.0
15	4.076541136376607E-4	0.0	0.0	8.80573650868711	0.0
16	4.076541136376607E-4	0.0	0.0	9.402134476939008	0.0
17	4.076541136376607E-4	0.0	0.0	10.110229672327623	0.0
18	4.076541136376607E-4	0.0	0.0	10.905970502148337	0.0
19	4.076541136376607E-4	0.0	0.0	11.318108811036012	0.0
20	4.076541136376607E-4	0.0	0.0	11.791395236969336	0.0
21	4.076541136376607E-4	0.0	0.0	12.369448770107539	0.0
22	4.076541136376607E-4	0.0	0.0	12.936495642177524	0.0
23	4.076541136376607E-4	0.0	0.0	13.492943507292932	0.0
24	4.076541136376607E-4	0.0	0.0	13.908750703203346	0.0
25	4.076541136376607E-4	0.0	0.0	14.288276683000008	0.0
26	4.076541136376607E-4	0.0	0.0	14.655165385273904	0.0
27	4.076541136376607E-4	0.0	0.0	15.008193847684117	0.0
28	4.076541136376607E-4	0.0	0.0	15.39628056386717	0.0
29	4.076541136376607E-4	0.0	0.0	15.749716680391021	0.0
30	4.076541136376607E-4	0.0	0.0	16.174492266801465	0.0
31	4.076541136376607E-4	0.0	0.0	16.578477493416386	0.0
32	4.076541136376607E-4	0.0	0.0	16.89848597262195	0.0
33	4.076541136376607E-4	0.0	0.0	17.2800502229868	0.0
34	4.076541136376607E-4	0.0	0.0	17.630632760715187	0.0
35	4.076541136376607E-4	0.0	0.0	18.01871947689824	0.0
36	4.076541136376607E-4	0.0	0.0	18.387238795626686	0.0
37	4.076541136376607E-4	0.0	0.0	18.732929483991423	0.0
38	4.076541136376607E-4	0.0	0.0	19.061906353697015	0.0
39	4.076541136376607E-4	0.0	0.0	19.418603703129968	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	20	7.022429E-4	45.0	1
CAACGGG	50	2.1827873E-11	45.0	3
CGATTGT	20	7.022429E-4	45.0	10
GCGTTCA	25	3.881807E-5	45.0	37
ACGGGCG	20	7.022429E-4	45.0	5
TAGTTGG	40	6.7775545E-9	45.0	2
AACGGGC	35	1.2067176E-7	45.0	4
CAGGCTT	25	3.881807E-5	45.0	36
TATTAGG	25	3.881807E-5	45.0	2
GCGATTA	20	7.022429E-4	45.0	9
GACGGGC	20	7.022429E-4	45.0	4
GCGATAG	20	7.022429E-4	45.0	9
AGTTATG	20	7.022429E-4	45.0	1
TAGGACG	20	7.022429E-4	45.0	1
TTATTAG	20	7.022429E-4	45.0	1
TCGGTAC	20	7.022429E-4	45.0	18
ACCTTAC	20	7.022429E-4	45.0	21
TCTGCGG	30	2.1585001E-6	44.999996	2
TATATGG	30	2.1585001E-6	44.999996	2
TACGGGC	30	2.1585001E-6	44.999996	4
>>END_MODULE