##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935665.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	812159
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37495244157856	33.0	31.0	34.0	30.0	34.0
2	32.57429887497399	34.0	31.0	34.0	31.0	34.0
3	32.42632292445199	34.0	31.0	34.0	30.0	34.0
4	36.06653992629522	37.0	35.0	37.0	35.0	37.0
5	36.06691546852279	37.0	35.0	37.0	35.0	37.0
6	36.06498481208729	37.0	35.0	37.0	35.0	37.0
7	36.19172846696275	37.0	37.0	37.0	35.0	37.0
8	35.7850938055233	37.0	36.0	37.0	35.0	37.0
9	37.66914237236797	39.0	38.0	39.0	35.0	39.0
10	37.61561960158048	39.0	37.0	39.0	35.0	39.0
11	37.588190243536054	39.0	37.0	39.0	35.0	39.0
12	37.521740201118256	39.0	37.0	39.0	35.0	39.0
13	37.48661407433766	39.0	37.0	39.0	35.0	39.0
14	38.92244622050608	41.0	38.0	41.0	35.0	41.0
15	38.95150210734597	41.0	39.0	41.0	35.0	41.0
16	39.0626810759962	41.0	39.0	41.0	35.0	41.0
17	38.915272748316525	41.0	38.0	41.0	35.0	41.0
18	38.28582334247358	39.0	38.0	40.0	35.0	41.0
19	37.595034962365744	37.0	37.0	40.0	35.0	41.0
20	36.59999581362763	35.0	35.0	40.0	34.0	41.0
21	36.68298695206234	35.0	35.0	40.0	33.0	41.0
22	36.63337228301355	35.0	35.0	40.0	33.0	41.0
23	36.52804684796943	35.0	35.0	40.0	33.0	41.0
24	36.43842154060966	35.0	35.0	40.0	33.0	41.0
25	36.46661675854112	35.0	35.0	40.0	33.0	41.0
26	36.24784309476346	35.0	35.0	40.0	33.0	41.0
27	36.08372498488596	35.0	35.0	40.0	33.0	41.0
28	35.936944613062224	35.0	35.0	40.0	33.0	41.0
29	35.71656288977897	35.0	35.0	40.0	31.0	41.0
30	35.19495197368988	35.0	35.0	40.0	30.0	41.0
31	34.544640889284	35.0	35.0	40.0	23.0	41.0
32	33.351578939591874	35.0	33.0	40.0	16.0	41.0
33	32.13323130076746	35.0	31.0	40.0	10.0	41.0
34	31.044451886884218	35.0	24.0	40.0	7.0	41.0
35	30.47159238523491	35.0	22.0	40.0	7.0	41.0
36	30.091784982989783	35.0	21.0	40.0	7.0	41.0
37	29.78992414047003	35.0	19.0	40.0	7.0	41.0
38	29.67019881574913	35.0	18.0	40.0	7.0	41.0
39	29.454832120311416	35.0	18.0	40.0	7.0	41.0
40	29.414578918660016	35.0	18.0	40.0	7.0	41.0
41	29.22172259372857	35.0	18.0	40.0	7.0	41.0
42	29.061310654687073	35.0	18.0	40.0	7.0	41.0
43	28.952170695639648	35.0	18.0	40.0	7.0	41.0
44	28.930175002678048	35.0	18.0	40.0	7.0	41.0
45	28.892625705065143	35.0	18.0	40.0	7.0	41.0
46	28.855205692481398	35.0	18.0	40.0	7.0	41.0
47	28.77369948495307	35.0	18.0	40.0	7.0	41.0
48	28.764467302584837	35.0	18.0	40.0	7.0	41.0
49	28.759487982033075	35.0	18.0	40.0	7.0	41.0
50	28.62449963615499	35.0	18.0	39.0	7.0	41.0
51	28.006296796563234	34.0	18.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	14.0
11	12.0
12	9.0
13	22.0
14	33.0
15	39.0
16	98.0
17	227.0
18	463.0
19	984.0
20	1855.0
21	3383.0
22	5866.0
23	9134.0
24	14822.0
25	26681.0
26	43960.0
27	54177.0
28	49764.0
29	38530.0
30	29377.0
31	25035.0
32	23065.0
33	24113.0
34	36655.0
35	50027.0
36	54072.0
37	51716.0
38	91869.0
39	175952.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.420397976258347	10.70122968532024	12.783703683638304	46.09466865478311
2	56.816953330567046	20.327177313801855	13.284098310798747	9.571771044832355
3	18.15408066647048	20.75110908085732	51.48917884305906	9.605631409613142
4	16.223547359568755	14.0856654916094	58.6757026641335	11.015084484688344
5	20.14100687180712	16.3759805653819	51.26003651994252	12.222976042868453
6	14.803997739358918	24.715480589391976	51.76855763465036	8.711964036598745
7	44.86830780672258	3.5522600870026686	48.47326693418407	3.106165172090687
8	44.251802910513824	8.90687660913688	43.36133688107871	3.4799835992705863
9	41.27467651038774	4.5809995333426095	43.63135789913059	10.512966057139057
10	27.610480213849755	14.410724993504967	48.26271702954717	9.716077763098113
11	21.618550062241507	14.841675090714995	51.568843046743304	11.970931800300187
12	17.437472219109804	12.773484009904465	53.787866661577354	16.001177109408378
13	17.59224486830781	13.022326908893456	56.62770467359224	12.757723549206498
14	12.51971596694736	19.427107253628908	53.83071541409995	14.222461365323785
15	11.545522490054287	14.926756952764176	58.29055640582694	15.237164151354598
16	13.247036602438685	15.472339775832072	54.32544612569706	16.95517749603218
17	12.961255123688833	17.88886166378751	55.09204478433411	14.057838428189553
18	13.39208209229966	15.895409642693117	54.12043700802429	16.592071256982933
19	14.012650232282104	16.72689707311007	52.771316946558485	16.489135748049335
20	17.737906001164795	15.829043327722772	54.89540841140712	11.537642259705304
21	14.906071348098093	19.318015314735167	53.815570596398	11.960342740768741
22	13.816875759549546	14.246225184969937	56.309294116053636	15.627604939426886
23	15.980245247543893	16.75447787933151	54.50102750816035	12.76424936496425
24	15.185942654086206	16.477192273926658	53.07901531596646	15.257849756020681
25	13.491323743257169	20.05333930917468	52.461402262364885	13.993934685203266
26	13.246667216641075	15.661219047009267	55.840297281689914	15.251816454659739
27	16.851503215503367	16.385953981917332	52.71012203275467	14.052420769824629
28	12.082609439777187	18.490098613695103	55.25999219364681	14.167299752880902
29	14.464162805559011	14.93512969750997	56.448552561752074	14.152154935178949
30	14.931435839533885	18.231651683968288	53.26309257177474	13.57381990472309
31	15.467660889062362	15.995882579642656	51.410130282370815	17.126326248924165
32	18.32584506235848	18.641916176512236	49.7206827727083	13.311555988420986
33	17.813014446678544	19.117561955232905	45.16049197263097	17.908931625457576
34	17.710448323542558	20.94023460923292	44.568735924862004	16.780581142362518
35	20.35364996263047	21.891279909475852	40.67097698849609	17.08409313939758
36	17.032748513529985	26.853731843148942	40.31291902201416	15.800600621306913
37	19.098600150955665	24.0417208945539	41.31358514773585	15.546093806754588
38	17.280360126526947	24.654162546988953	38.39716114701678	19.668316179467322
39	19.329343145861834	24.429083467646116	39.31594675426856	16.92562663222349
40	18.622954372234993	22.997959759111207	39.142951072388534	19.236134796265265
41	17.80722740251601	26.11163577575327	37.25883724738629	18.822299574344434
42	18.92868268405571	24.589027518010635	39.231603663814596	17.250686134119057
43	18.19692941899308	25.960187598733746	37.62846930219329	18.214413680079886
44	18.334587192901882	23.589962064078584	39.41962103479737	18.655829708222157
45	17.70109055000314	21.98978278883815	39.72337928903084	20.585747372127873
46	19.550851495827786	24.2137315476403	39.25130423968706	16.984112716844855
47	16.242386035246792	23.483332697168905	43.300511353072494	16.973769914511813
48	17.393761566392786	22.753672618292722	41.008349350311946	18.844216465002543
49	17.15747778452249	21.06262443684057	43.850649934310894	17.92924784432605
50	16.902232198374946	21.51684583929994	42.13485290441896	19.446069057906147
51	16.30074899126895	20.94491349600263	41.436713746938715	21.317623765789705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	462.0
2	724.0
3	51470.5
4	102217.0
5	68147.5
6	34078.0
7	32221.0
8	30364.0
9	29078.0
10	27792.0
11	26159.5
12	24527.0
13	22707.5
14	20888.0
15	19329.5
16	17771.0
17	16114.5
18	14458.0
19	13064.0
20	11670.0
21	10544.0
22	9418.0
23	8560.5
24	7703.0
25	7440.0
26	6918.5
27	6660.0
28	7129.0
29	7598.0
30	8110.0
31	8622.0
32	9671.0
33	10720.0
34	11770.5
35	12821.0
36	13056.0
37	13291.0
38	14171.5
39	15052.0
40	16941.0
41	18830.0
42	21001.5
43	23173.0
44	26169.0
45	29165.0
46	48146.0
47	67127.0
48	56376.0
49	45625.0
50	44642.5
51	43660.0
52	39012.0
53	34364.0
54	33149.0
55	31934.0
56	29418.5
57	26903.0
58	25550.5
59	24198.0
60	23945.0
61	23692.0
62	21950.5
63	20209.0
64	17257.0
65	14305.0
66	11546.0
67	8787.0
68	7404.5
69	6022.0
70	4977.0
71	3932.0
72	3458.0
73	2984.0
74	2330.5
75	1326.5
76	976.0
77	724.5
78	473.0
79	341.5
80	210.0
81	151.5
82	93.0
83	69.0
84	45.0
85	28.5
86	12.0
87	10.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	812159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.8369569321011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.33074826957228	32.00132559138406
2	7.119623422401422	5.6724826330196745
3	2.5781704087228214	3.0811939060772557
4	1.4870329926586208	2.3695547714061957
5	1.1064090778584534	2.203798539196645
6	0.912167719018381	2.1802791682432883
7	0.7699316931766733	2.1470214991217174
8	0.650908759195948	2.074417936545308
9	0.6181662125081596	2.216327470611085
>10	4.277734186400293	26.89617323718344
>50	0.07954467837549016	2.199780828312176
>100	0.062387964225563725	4.406519599696783
>500	0.0031193982112774905	0.8911210391827838
>1k	0.0028074583901497416	1.6798457966354585
>5k	9.358194633832472E-4	3.1141393448501695
>10k+	3.119398211277491E-4	6.866018638533982
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55252	6.803101363156721	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC	8711	1.0725732276561608	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC	8572	1.0554583523669625	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG	7777	0.9575711160006846	No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	2325	0.2862739931466622	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT	1983	0.2441640122192822	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT	1618	0.1992220735102363	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	1564	0.19257312915328156	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC	1316	0.1620372365509709	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1293	0.15920527876930501	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA	1292	0.15908215017010216	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1098	0.13519520192474627	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	1029	0.12669932857974853	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	988	0.12165105601243108	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCTCCGATCGAATGC	870	0.10712188130649294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	866	0.10662936690968147	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.156429960143272E-4	0.0	0.0	0.09099203481091757	0.0
2	6.156429960143272E-4	0.0	0.0	0.5686078711188326	0.0
3	7.387715952171927E-4	0.0	0.0	0.7593340712840713	0.0
4	7.387715952171927E-4	0.0	0.0	1.1353688132496222	0.0
5	7.387715952171927E-4	0.0	0.0	2.725205286157021	0.0
6	8.619001944200581E-4	0.0	0.0	3.1475363814228494	0.0
7	8.619001944200581E-4	0.0	0.0	3.6580275537179294	0.0
8	8.619001944200581E-4	0.0	0.0	4.25101488747893	0.0
9	8.619001944200581E-4	0.0	0.0	4.429428227723881	0.0
10	8.619001944200581E-4	0.0	0.0	5.781872761368156	0.0
11	8.619001944200581E-4	0.0	0.0	6.363409135403289	0.0
12	8.619001944200581E-4	0.0	0.0	7.930589946057361	0.0
13	8.619001944200581E-4	0.0	0.0	8.18017161664157	0.0
14	9.850287936229237E-4	0.0	0.0	8.310195417399795	0.0
15	0.001108157392825789	0.0	0.0	8.629591003732028	0.0
16	0.001108157392825789	0.0	0.0	8.965239565159038	0.0
17	0.001108157392825789	0.0	0.0	9.30002622639163	0.0
18	0.001108157392825789	0.0	0.0	9.635551659219438	0.0
19	0.001108157392825789	0.0	0.0	10.150598589685025	0.0
20	0.0012312859920286545	0.0	0.0	10.422343408125748	0.0
21	0.0013544145912315199	0.0	0.0	10.701106556721037	0.0
22	0.0013544145912315199	0.0	0.0	11.074186212305719	0.0
23	0.0013544145912315199	0.0	0.0	11.355042547087455	0.0
24	0.0013544145912315199	0.0	0.0	11.607825561250937	0.0
25	0.0013544145912315199	0.0	0.0	11.822069323863923	0.0
26	0.0013544145912315199	0.0	0.0	12.025108383949448	0.0
27	0.0013544145912315199	0.0	0.0	12.295252530600536	0.0
28	0.0013544145912315199	0.0	0.0	12.506541206832653	0.0
29	0.0013544145912315199	0.0	0.0	12.724109441624115	0.0
30	0.0013544145912315199	0.0	0.0	12.977754355982018	0.0
31	0.0013544145912315199	0.0	0.0	13.205296007308913	0.0
32	0.0013544145912315199	0.0	0.0	13.434315201826243	0.0
33	0.0013544145912315199	0.0	0.0	13.651637179419302	0.0
34	0.0013544145912315199	0.0	0.0	13.86132518386178	0.0
35	0.0013544145912315199	0.0	0.0	14.081848505034113	0.0
36	0.0013544145912315199	0.0	0.0	14.326997546047018	0.0
37	0.0014775431904343855	0.0	0.0	14.566605800095793	0.0
38	0.0014775431904343855	0.0	0.0	14.810154169319063	0.0
39	0.0014775431904343855	0.0	0.0	15.038927106637987	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGACG	20	7.032063E-4	45.0	1
TTACGAG	20	7.032063E-4	45.0	1
TGCGTAG	20	7.032063E-4	45.0	1
CTTACCG	20	7.032063E-4	45.0	1
CGTTTTT	30710	0.0	44.41387	1
GATGAAT	1795	0.0	41.866295	20
CGATGAA	1705	0.0	41.832844	19
ATGAATG	1800	0.0	41.75	21
CCGATGA	1655	0.0	41.73716	18
TACGGCT	990	0.0	41.59091	7
CGATCGA	260	0.0	41.53846	41
ACGGCTG	995	0.0	41.381912	8
CGTAAGG	55	6.002665E-11	40.909092	2
GTTTTTT	33875	0.0	40.43026	2
ACCGATG	1725	0.0	40.173912	17
ATCGAAT	265	0.0	39.905663	43
CTCCGAT	210	0.0	39.642857	38
CGACCCG	80	0.0	39.375	32
CGGCTGT	1090	0.0	39.013763	9
CGGGTAT	35	6.246024E-6	38.57143	6
>>END_MODULE