SRR2935664.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935664.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357097
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25245	7.069507724791864	No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	911	0.2551127564779318	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	739	0.20694657193983707	TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	518	0.14505862552751772	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	509	0.14253830191796626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	413	0.11565485008275063	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	400	0.11201438264673184	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	383	0.10725377138424573	TruSeq Adapter, Index 27 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	30	2.1612832E-6	45.000004	2
CTCGCGG	30	2.1612832E-6	45.000004	2
TTAACGG	30	2.1612832E-6	45.000004	2
CGATGCA	30	2.1612832E-6	45.000004	10
GCATAAG	20	7.026748E-4	45.0	9
ATCCTGC	35	1.2087912E-7	45.0	20
GTACCAT	20	7.026748E-4	45.0	42
GGATATC	20	7.026748E-4	45.0	8
TACGGGT	20	7.026748E-4	45.0	4
ACGGGCG	20	7.026748E-4	45.0	5
TGCGAAG	40	6.7921064E-9	45.0	1
TCAGACG	20	7.026748E-4	45.0	22
ACTACGG	25	3.885386E-5	45.0	2
CATACGG	25	3.885386E-5	45.0	2
CTATTAG	20	7.026748E-4	45.0	30
TTACCGG	20	7.026748E-4	45.0	2
AGTACGG	25	3.885386E-5	45.0	2
ATATTGC	20	7.026748E-4	45.0	33
CGTTTTT	12960	0.0	44.670135	1
AGGGACG	55	6.002665E-11	40.909092	6