##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935664.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357097
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35052380725685	33.0	31.0	34.0	30.0	34.0
2	32.55373469953542	34.0	31.0	34.0	31.0	34.0
3	32.406029734217874	34.0	31.0	34.0	30.0	34.0
4	36.03352590472617	37.0	35.0	37.0	35.0	37.0
5	36.07044584524653	37.0	35.0	37.0	35.0	37.0
6	36.10070092999941	37.0	36.0	37.0	35.0	37.0
7	36.255936062190386	37.0	37.0	37.0	35.0	37.0
8	36.0414033161858	37.0	37.0	37.0	35.0	37.0
9	37.8570836495406	39.0	38.0	39.0	35.0	39.0
10	37.66709045441435	39.0	38.0	39.0	35.0	39.0
11	37.640008177049936	39.0	37.0	39.0	35.0	39.0
12	37.612301419502266	39.0	37.0	39.0	35.0	39.0
13	37.60609302234407	39.0	37.0	39.0	35.0	39.0
14	39.00801182871881	41.0	39.0	41.0	35.0	41.0
15	39.111244283766034	41.0	39.0	41.0	35.0	41.0
16	39.15431661425327	41.0	39.0	41.0	35.0	41.0
17	39.12864291775064	41.0	39.0	41.0	35.0	41.0
18	38.50254412666586	39.0	38.0	41.0	35.0	41.0
19	37.80917789844216	37.0	37.0	41.0	35.0	41.0
20	37.013254101826675	37.0	35.0	40.0	34.0	41.0
21	36.964435433509664	37.0	35.0	40.0	34.0	41.0
22	36.933133014279036	36.0	35.0	40.0	34.0	41.0
23	36.93128757732493	36.0	35.0	40.0	34.0	41.0
24	36.74760639266082	36.0	35.0	40.0	34.0	41.0
25	36.612715872718056	35.0	35.0	40.0	33.0	41.0
26	36.41382593525009	35.0	35.0	40.0	33.0	41.0
27	36.30476593194566	35.0	35.0	40.0	33.0	41.0
28	36.12934020728262	35.0	35.0	40.0	33.0	41.0
29	35.863628089846735	35.0	35.0	40.0	32.0	41.0
30	35.342985239304724	35.0	35.0	40.0	30.0	41.0
31	34.64742072882158	35.0	35.0	40.0	24.0	41.0
32	33.66227943667967	35.0	33.0	40.0	18.0	41.0
33	32.47030358698057	35.0	32.0	40.0	12.0	41.0
34	31.532485571147337	35.0	30.0	40.0	8.0	41.0
35	30.954284130082304	35.0	24.0	40.0	7.0	41.0
36	30.625054256966596	35.0	23.0	40.0	7.0	41.0
37	30.459774235011775	35.0	23.0	40.0	7.0	41.0
38	30.315485708364953	35.0	22.0	40.0	7.0	41.0
39	30.17706113464969	35.0	21.0	40.0	7.0	41.0
40	30.060896619125895	35.0	21.0	40.0	7.0	41.0
41	29.882841356830216	35.0	21.0	40.0	7.0	41.0
42	29.758354172675773	35.0	20.0	40.0	7.0	41.0
43	29.634673492076384	35.0	18.0	40.0	7.0	41.0
44	29.51083599134129	35.0	18.0	40.0	7.0	41.0
45	29.470239178710546	35.0	20.0	40.0	7.0	41.0
46	29.425402621696627	35.0	20.0	40.0	7.0	41.0
47	29.446181289677593	35.0	20.0	40.0	7.0	41.0
48	29.4158113901825	35.0	20.0	40.0	7.0	41.0
49	29.3991492507638	35.0	20.0	40.0	7.0	41.0
50	29.27584101798671	35.0	20.0	40.0	7.0	41.0
51	28.584799088202924	35.0	19.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	3.0
11	5.0
12	5.0
13	4.0
14	10.0
15	9.0
16	27.0
17	75.0
18	147.0
19	320.0
20	585.0
21	958.0
22	1605.0
23	2738.0
24	5553.0
25	11499.0
26	19233.0
27	22612.0
28	19653.0
29	15231.0
30	11405.0
31	10125.0
32	9796.0
33	10917.0
34	16573.0
35	24653.0
36	25693.0
37	26502.0
38	50648.0
39	70433.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.313018591587163	13.615348210710254	15.560758001327372	44.51087519637522
2	57.94420003528453	15.353811429387534	15.688454397544646	11.013534137783292
3	20.02201082619008	15.447623474854172	53.15250478161396	11.377860917341787
4	18.264225126506243	16.66577988613738	52.63499833378605	12.434996653570318
5	15.864036942343397	18.63527276902354	52.27823252505621	13.222457763576841
6	16.155554373181516	21.988143277596844	51.563020691856835	10.293281657364805
7	51.03123241024148	3.8588954821799115	41.74047947756492	3.3693926300136936
8	51.01863079219372	3.4674052148295837	41.439720860158445	4.074243132818254
9	47.051081358846474	4.801776548108777	43.195546308145964	4.951595784898781
10	25.43762619120295	16.575888344063376	47.78729588879212	10.199189575941551
11	18.68651934908442	16.62041406116545	51.73020215795708	12.962864431793042
12	18.18329473504398	14.222466164655541	54.33649680618991	13.257742294110564
13	15.608924185865467	16.02197722187529	55.55409314555989	12.815005446699356
14	12.827327028790497	18.390241306983818	54.2496856596387	14.532746004586988
15	12.021663581603878	17.504207540248167	57.6426013100082	12.83152756813975
16	13.218817296140825	16.052501141146525	56.63671215384055	14.0919694088721
17	13.209576109572469	16.251326670344472	54.06654214401129	16.472555076071767
18	13.73940413949151	16.864045343422095	54.880606669896416	14.51594384718998
19	14.790939156587706	18.108805170583903	53.16762672327127	13.932628949557124
20	16.149673618092564	17.216050540889448	53.48182706659536	13.152448774422638
21	15.148825109144015	17.71619475940711	53.663570402439674	13.471409729009205
22	13.146007947420449	16.60473204759491	53.142703523132376	17.10655648185227
23	13.386838870110923	17.401154308213172	53.4793067429858	15.732700078690105
24	14.350442596829433	15.808869858889881	54.01081498864454	15.829872555636143
25	13.30758869438836	17.55349386861273	53.11918050277655	16.019736934222355
26	13.002349501676017	18.08080157492222	54.149992859083106	14.76685606431866
27	13.530777351811974	17.97410787545121	53.79070672674371	14.704408045993105
28	12.032584983911935	17.003783285773892	55.27993794403201	15.683693786282157
29	12.99394842297751	15.812510326325901	54.806117105436336	16.38742414526025
30	13.418482933208626	16.888968543560992	53.08305586437299	16.6094926588574
31	13.397200200505747	18.272066133291514	51.03039230237163	17.300341363831116
32	13.906865641548375	19.1088135716626	49.82455747317955	17.159763313609467
33	14.47001795030482	19.819544829556115	46.775525977535516	18.93491124260355
34	13.93822966868946	20.59216403386195	45.7968003091597	19.672805988288896
35	14.291074974026666	20.812272295762778	43.77746102599574	21.11919170421482
36	15.27204093005542	22.528612673867325	42.40276451496372	19.796581881113536
37	15.299204417847251	23.164294295387528	42.17509528223424	19.36140600453098
38	15.262799743487063	23.47345399149251	41.40723668919089	19.856509575829538
39	16.238445016340098	22.21805279797926	41.08939587843079	20.45410630724985
40	16.330576846067036	22.570898103316466	42.15073215400857	18.947792896607922
41	14.76069527327309	23.217781163101343	40.762873953015564	21.258649610610004
42	15.74278137312831	23.185857064047024	40.522323066281714	20.549038496542956
43	16.69350344584245	22.464764475758685	39.91520511233642	20.926526966062443
44	16.249086382691537	22.724917879455724	39.651971313116604	21.374024424736135
45	16.13511174834849	21.892931052347123	39.918845579772444	22.053111619531947
46	16.40618655435358	22.35331016502519	40.795918195896355	20.444585084724878
47	14.712529088734993	21.36954384943027	43.07681106254043	20.84111599929431
48	15.009927274662067	20.58488309898991	42.410605521749	21.99458410459903
49	15.094218097603731	19.947521261730007	43.264715189430326	21.693545451235938
50	14.764895812622342	19.990366763092382	42.648636084873296	22.59610133941198
51	14.979123319434215	19.806943211508358	40.80963995777058	24.40429351128685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	170.5
2	262.0
3	20216.0
4	40170.0
5	26742.0
6	13314.0
7	12808.5
8	12303.0
9	11853.5
10	11404.0
11	10938.5
12	10473.0
13	9954.5
14	9436.0
15	8799.5
16	8163.0
17	7499.0
18	6835.0
19	6230.5
20	5626.0
21	5121.5
22	4617.0
23	4169.5
24	3722.0
25	3602.0
26	3315.5
27	3149.0
28	3212.0
29	3275.0
30	3610.0
31	3945.0
32	4387.0
33	4829.0
34	5315.0
35	5801.0
36	6326.5
37	6852.0
38	7140.5
39	7429.0
40	8699.5
41	9970.0
42	11402.5
43	12835.0
44	14585.0
45	16335.0
46	19767.5
47	23200.0
48	24940.0
49	26680.0
50	26449.0
51	26218.0
52	22191.5
53	18165.0
54	15245.5
55	12326.0
56	11022.0
57	9718.0
58	9539.0
59	9360.0
60	8681.5
61	8003.0
62	7361.0
63	6719.0
64	5888.5
65	5058.0
66	3849.0
67	2640.0
68	2245.0
69	1850.0
70	1521.5
71	1193.0
72	950.0
73	707.0
74	568.5
75	354.0
76	278.0
77	211.5
78	145.0
79	96.5
80	48.0
81	46.5
82	45.0
83	24.5
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	357097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.95936475947322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78272342526067	39.222474637751915
2	7.9980210062260415	7.671600135830474
3	2.6717712102505993	3.844093500788031
4	1.3469214693543337	2.583899922045205
5	0.848968421344508	2.035799309426771
6	0.6266486884358038	1.803220381484292
7	0.538207665949175	1.8068468437320837
8	0.48237731707893056	1.8507607761187599
9	0.386894578270266	1.6699696382453624
>10	3.215130982419953	25.114855414093075
>50	0.07528109271517702	2.385424620086313
>100	0.02411347501033014	2.135232869108832
>500	0.002352534147349282	0.7550882946637176
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.881335368373205E-4	7.12073365662516
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25245	7.069507724791864	No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	911	0.2551127564779318	TruSeq Adapter, Index 16 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	739	0.20694657193983707	TruSeq Adapter, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	518	0.14505862552751772	TruSeq Adapter, Index 27 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	509	0.14253830191796626	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	413	0.11565485008275063	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	400	0.11201438264673184	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTG	383	0.10725377138424573	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15569999187895725	0.0
2	0.0	0.0	0.0	0.8244258562799464	0.0
3	0.0	0.0	0.0	1.2044346494089841	0.0
4	0.0	0.0	0.0	1.6496918204297433	0.0
5	0.0	0.0	0.0	2.8474056068799234	0.0
6	0.0	0.0	0.0	3.5502958579881656	0.0
7	0.0	0.0	0.0	4.23274348426338	0.0
8	0.0	0.0	0.0	5.351487130947613	0.0
9	0.0	0.0	0.0	5.76790059843684	0.0
10	2.800359566168296E-4	0.0	0.0	6.775469970344192	0.0
11	2.800359566168296E-4	0.0	0.0	8.113481771059403	0.0
12	2.800359566168296E-4	0.0	0.0	9.215983332259862	0.0
13	2.800359566168296E-4	0.0	0.0	9.622875577224116	0.0
14	2.800359566168296E-4	0.0	0.0	9.781655964625857	0.0
15	2.800359566168296E-4	0.0	0.0	10.082694617988949	0.0
16	2.800359566168296E-4	0.0	0.0	10.749180194737004	0.0
17	2.800359566168296E-4	0.0	0.0	11.581167021845605	0.0
18	2.800359566168296E-4	0.0	0.0	12.456559422229814	0.0
19	2.800359566168296E-4	0.0	0.0	12.991148063411343	0.0
20	8.401078698504888E-4	0.0	0.0	13.485691562796664	0.0
21	8.401078698504888E-4	0.0	0.0	14.132294586624923	0.0
22	8.401078698504888E-4	0.0	0.0	14.812221889290585	0.0
23	8.401078698504888E-4	0.0	0.0	15.50783120552679	0.0
24	0.0011201438264673185	0.0	0.0	16.01021571169738	0.0
25	0.0011201438264673185	0.0	0.0	16.515960649347377	0.0
26	0.0011201438264673185	0.0	0.0	16.94217537531819	0.0
27	0.0011201438264673185	0.0	0.0	17.38855269016542	0.0
28	0.0011201438264673185	0.0	0.0	17.83717029266558	0.0
29	0.0011201438264673185	0.0	0.0	18.283547607512805	0.0
30	0.0011201438264673185	0.0	0.0	18.78285171816061	0.0
31	0.0011201438264673185	0.0	0.0	19.276275073719464	0.0
32	0.0011201438264673185	0.0	0.0	19.726012820046094	0.0
33	0.0011201438264673185	0.0	0.0	20.140185999882384	0.0
34	0.0011201438264673185	0.0	0.0	20.549878604412807	0.0
35	0.001400179783084148	0.0	0.0	21.004096926045303	0.0
36	0.001400179783084148	0.0	0.0	21.431711831799202	0.0
37	0.001400179783084148	0.0	0.0	21.88172961408245	0.0
38	0.001400179783084148	0.0	0.0	22.27714038482541	0.0
39	0.001400179783084148	0.0	0.0	22.66583029260957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	30	2.1612832E-6	45.000004	2
CTCGCGG	30	2.1612832E-6	45.000004	2
TTAACGG	30	2.1612832E-6	45.000004	2
CGATGCA	30	2.1612832E-6	45.000004	10
GCATAAG	20	7.026748E-4	45.0	9
ATCCTGC	35	1.2087912E-7	45.0	20
GTACCAT	20	7.026748E-4	45.0	42
GGATATC	20	7.026748E-4	45.0	8
TACGGGT	20	7.026748E-4	45.0	4
ACGGGCG	20	7.026748E-4	45.0	5
TGCGAAG	40	6.7921064E-9	45.0	1
TCAGACG	20	7.026748E-4	45.0	22
ACTACGG	25	3.885386E-5	45.0	2
CATACGG	25	3.885386E-5	45.0	2
CTATTAG	20	7.026748E-4	45.0	30
TTACCGG	20	7.026748E-4	45.0	2
AGTACGG	25	3.885386E-5	45.0	2
ATATTGC	20	7.026748E-4	45.0	33
CGTTTTT	12960	0.0	44.670135	1
AGGGACG	55	6.002665E-11	40.909092	6
>>END_MODULE