##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	485718
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48012426963794	34.0	31.0	34.0	31.0	34.0
2	32.684246826347795	34.0	31.0	34.0	31.0	34.0
3	32.52361040768512	34.0	31.0	34.0	31.0	34.0
4	36.129457833557744	37.0	37.0	37.0	35.0	37.0
5	36.13824688399442	37.0	35.0	37.0	35.0	37.0
6	36.142181265672676	37.0	37.0	37.0	35.0	37.0
7	36.27742434910792	37.0	37.0	37.0	35.0	37.0
8	36.09552456363569	37.0	37.0	37.0	35.0	37.0
9	37.902206630184594	39.0	38.0	39.0	35.0	39.0
10	37.74792369234824	39.0	38.0	39.0	35.0	39.0
11	37.70635636315722	39.0	38.0	39.0	35.0	39.0
12	37.56626478738692	39.0	37.0	39.0	35.0	39.0
13	37.53422356181982	39.0	37.0	39.0	35.0	39.0
14	38.79945565122149	41.0	38.0	41.0	35.0	41.0
15	38.925611157091154	41.0	39.0	41.0	35.0	41.0
16	39.02070337109187	41.0	39.0	41.0	35.0	41.0
17	39.01451459488839	41.0	39.0	41.0	35.0	41.0
18	38.34083151128844	39.0	38.0	40.0	35.0	41.0
19	37.63419926788795	37.0	37.0	40.0	35.0	41.0
20	36.81369024825104	35.0	35.0	40.0	34.0	41.0
21	36.753719236264665	35.0	35.0	40.0	33.0	41.0
22	36.75513981363672	35.0	35.0	40.0	34.0	41.0
23	36.76348210278392	35.0	35.0	40.0	34.0	41.0
24	36.60721653305004	35.0	35.0	40.0	34.0	41.0
25	36.50722641532741	35.0	35.0	40.0	33.0	41.0
26	36.352251718075095	35.0	35.0	40.0	33.0	41.0
27	36.26696972317271	35.0	35.0	40.0	33.0	41.0
28	36.12186083282892	35.0	35.0	40.0	33.0	41.0
29	35.83079070571813	35.0	35.0	40.0	33.0	41.0
30	35.287158392318176	35.0	35.0	40.0	31.0	41.0
31	34.57073240028164	35.0	35.0	40.0	23.0	41.0
32	33.53157593500755	35.0	33.0	40.0	17.0	41.0
33	32.33454391231126	35.0	32.0	40.0	12.0	41.0
34	31.306605890660837	35.0	28.0	40.0	8.0	41.0
35	30.667916362992518	35.0	23.0	40.0	7.0	41.0
36	30.300577701464636	35.0	22.0	40.0	7.0	41.0
37	30.10025570392697	35.0	21.0	40.0	7.0	41.0
38	29.904133674271904	35.0	21.0	40.0	7.0	41.0
39	29.736509661984936	35.0	20.0	40.0	7.0	41.0
40	29.555106049189035	35.0	19.0	40.0	7.0	41.0
41	29.36489485668639	35.0	18.0	40.0	7.0	41.0
42	29.26008095232213	35.0	18.0	40.0	7.0	41.0
43	29.163728747956633	35.0	18.0	40.0	7.0	41.0
44	29.04862286347222	35.0	18.0	40.0	7.0	41.0
45	29.010355803161506	35.0	18.0	40.0	7.0	41.0
46	29.01302195924384	35.0	18.0	40.0	7.0	41.0
47	29.068535652374422	35.0	20.0	39.0	7.0	41.0
48	29.037733829094247	35.0	20.0	39.0	7.0	41.0
49	29.03045388476441	35.0	20.0	39.0	7.0	41.0
50	28.91144244191074	35.0	20.0	39.0	7.0	41.0
51	28.28742809613809	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	6.0
10	7.0
11	11.0
12	13.0
13	21.0
14	16.0
15	34.0
16	59.0
17	136.0
18	297.0
19	515.0
20	883.0
21	1339.0
22	2382.0
23	4050.0
24	8119.0
25	16261.0
26	26747.0
27	31170.0
28	28392.0
29	21090.0
30	15980.0
31	13645.0
32	13545.0
33	16164.0
34	27282.0
35	33258.0
36	33768.0
37	34016.0
38	58268.0
39	98122.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.865831614228835	12.850254674523075	14.657064387154687	46.626849324093406
2	59.83533655330871	14.237067598894832	15.246501056168393	10.681094791628064
3	20.146669466645257	14.828974837251243	54.12811549088155	10.896240205221961
4	18.140155398811654	16.010318744621365	53.833294215985404	12.016231640581571
5	16.02370099522768	18.09980276621414	53.65788379265335	12.218612445904824
6	16.736872012155203	20.652106778006992	52.90477190468543	9.706249305152372
7	50.33517390749365	3.6706484009239935	42.763290633659864	3.2308870579224984
8	51.32010755211872	2.838066532432399	42.67867363367222	3.163152281776669
9	47.65851790545131	4.001498812067908	44.11510382567663	4.224879456804153
10	28.053109005637015	13.898599598944244	48.36057136033666	9.687720035082084
11	20.880428561428648	14.887239097583372	52.02936683425362	12.20296550673436
12	18.757385972930795	13.436191370301287	54.499936176958656	13.306486479809271
13	17.047546107000358	13.147546518761915	56.47495048567276	13.32995688856497
14	14.058980725441511	14.247979280158447	57.621088779909336	14.071951214490714
15	13.256251569840938	14.480418679151278	58.50267027369791	13.760659477309881
16	16.889223788288675	14.993473579319689	55.51925191160303	12.598050720788606
17	16.90981186614455	15.272236153488238	54.435289612491204	13.38266236787601
18	17.139163053459004	14.841945326300445	55.13590190192663	12.882989718313919
19	16.564755681280086	16.557961615587647	53.68444241308743	13.192840290044842
20	16.381521788362797	17.564512741961387	53.295739503168505	12.758225966507315
21	16.221346542644085	16.561255708044587	55.05149078271755	12.165906966593786
22	15.799908588934318	14.429154365290147	55.66975076072948	14.101186285046055
23	15.009738160825828	15.748850155851748	55.42578203813735	13.815629645185066
24	15.288912496551497	15.89379022395711	54.87875680950675	13.938540469984643
25	16.6510197274962	15.793732165577556	53.32497457372385	14.230273533202393
26	14.81497494430925	15.610910034217385	53.605384194120866	15.968730827352498
27	14.54732993218287	15.208001350577907	55.45213477779287	14.792533939446345
28	14.296361263119753	16.134464854092293	53.690412955665636	15.87876092712232
29	15.200177880992674	17.178280401385166	52.81603728912662	14.805504428495547
30	16.93348815567881	15.521351895544328	52.92556586331987	14.619594085456994
31	16.751283666654313	16.524608929461127	51.89430904351908	14.829798360365478
32	18.054920756488333	17.608777109351518	49.26562326288093	15.07067887127922
33	18.600504819669027	19.341675622480533	46.366204258438025	15.691615299412417
34	17.28657369090707	21.674510724329753	45.239830518943094	15.799085065820083
35	18.270272050860786	23.986140105987424	41.79812154377643	15.945466299375358
36	20.657047916692402	24.873280380797087	38.81490906246011	15.654762640050398
37	20.31940343985605	25.400746935464614	37.901827809552046	16.378021815127298
38	21.192543821723717	24.59266487962151	37.774593488402736	16.440197810252037
39	20.602283629595775	23.636142782437545	37.35994960038541	18.401623987581274
40	21.703951675663657	22.85358994313573	38.2983541890562	17.144104192144415
41	20.003170563989805	23.380438855467574	37.68709415751527	18.929296423027353
42	20.317344632070462	23.771818215507764	38.17853157593501	17.732305576486766
43	19.555173989845958	23.377350643789192	38.67655717926863	18.39091818709622
44	20.068023009235812	23.160558183966828	38.32305988248325	18.44835892431411
45	19.41002804096204	22.450269497939132	38.47232344693835	19.66737901416048
46	18.632210459567077	22.708855755808926	39.4239044054369	19.2350293791871
47	17.883010306391775	22.81385495287389	40.750188380912384	18.552946359821952
48	17.795305094725748	22.839384169415176	40.74895309624103	18.61635763961805
49	18.916737695535268	22.00886110870917	40.67957127386673	18.394829921888835
50	17.45436652543245	22.380681794786277	41.16153817647277	19.003413503308504
51	17.460748829567773	21.852597597783074	40.89677549524621	19.789878077402935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	180.0
1	336.5
2	493.0
3	29750.5
4	59008.0
5	38633.0
6	18258.0
7	17540.5
8	16823.0
9	16257.0
10	15691.0
11	15191.0
12	14691.0
13	13723.5
14	12756.0
15	11665.0
16	10574.0
17	9760.5
18	8947.0
19	8173.0
20	7399.0
21	6761.5
22	6124.0
23	5692.5
24	5261.0
25	5025.5
26	4805.5
27	4821.0
28	4920.0
29	5019.0
30	5277.5
31	5536.0
32	5931.0
33	6326.0
34	7167.0
35	8008.0
36	8335.5
37	8663.0
38	9231.5
39	9800.0
40	10309.0
41	10818.0
42	11740.0
43	12662.0
44	14022.5
45	15383.0
46	18020.0
47	20657.0
48	23910.0
49	27163.0
50	27691.0
51	28219.0
52	25715.5
53	23212.0
54	21833.5
55	20455.0
56	19583.0
57	18711.0
58	18300.0
59	17889.0
60	16818.0
61	15747.0
62	14223.5
63	12700.0
64	11386.0
65	10072.0
66	8935.0
67	7798.0
68	6716.0
69	5634.0
70	4647.0
71	3660.0
72	3016.5
73	2373.0
74	2012.0
75	1295.0
76	939.0
77	695.0
78	451.0
79	336.0
80	221.0
81	151.0
82	81.0
83	59.5
84	38.0
85	26.0
86	14.0
87	7.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485718.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.95895071154324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30939720845592	37.84986967467911
2	7.876439072973299	7.71244384712346
3	4.026304160719216	5.913708808630007
4	2.719892327813792	5.3265229767256015
5	2.056600343129494	5.034449741630991
6	1.525269937150567	4.480536940485195
7	1.1844071124054687	4.059113060706241
8	0.8727136757590409	3.4181716669421283
9	0.6398906030501217	2.819553524595952
>10	1.6986868171274299	11.218947166992505
>50	0.06463087587575468	2.196989056177856
>100	0.0236557454136782	2.210845618646516
>500	0.0016896961009770144	0.5052475066747838
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.224240252442536E-4	7.25360040998964
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35073	7.220856546391116	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	682	0.140410690977069	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	628	0.1292931289348964	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTG	621	0.12785196348498512	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	512	0.10541095862208112	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.235231142350088E-4	0.0	0.0	0.04941138685410053	0.0
2	8.235231142350088E-4	0.0	0.0	0.17643982722485063	0.0
3	8.235231142350088E-4	0.0	0.0	0.3961146179470392	0.0
4	8.235231142350088E-4	0.0	0.0	0.5935542845848826	0.0
5	8.235231142350088E-4	0.0	0.0	1.0571977978991924	0.0
6	8.235231142350088E-4	0.0	0.0	1.7022222771237632	0.0
7	8.235231142350088E-4	0.0	0.0	2.286306045894943	0.0
8	8.235231142350088E-4	0.0	0.0	3.152858242848731	0.0
9	8.235231142350088E-4	0.0	0.0	3.812088495793856	0.0
10	8.235231142350088E-4	0.0	0.0	4.499524415401529	0.0
11	8.235231142350088E-4	0.0	0.0	5.158754668346654	0.0
12	8.235231142350088E-4	0.0	0.0	5.627133439567815	0.0
13	8.235231142350088E-4	0.0	0.0	5.849896441968385	0.0
14	8.235231142350088E-4	0.0	0.0	6.037453831235408	0.0
15	8.235231142350088E-4	0.0	0.0	6.180540972333741	0.0
16	8.235231142350088E-4	0.0	0.0	6.353686707101652	0.0
17	8.235231142350088E-4	0.0	0.0	6.5395970501402045	0.0
18	0.001029403892793761	0.0	0.0	6.762565933319333	0.0
19	0.001029403892793761	0.0	0.0	6.899682531839463	0.0
20	0.001029403892793761	0.0	0.0	7.056975446658349	0.0
21	0.001029403892793761	0.0	0.0	7.247209286046636	0.0
22	0.001029403892793761	0.0	0.0	7.4545312300552995	0.0
23	0.001029403892793761	0.0	0.0	7.646000354114939	0.0
24	0.001029403892793761	0.0	0.0	7.819351969661408	0.0
25	0.001029403892793761	0.0	0.0	7.973350792023355	0.0
26	0.001029403892793761	0.0	0.0	8.116643813900247	0.0
27	0.001029403892793761	0.0	0.0	8.301936514603124	0.0
28	0.001029403892793761	0.0	0.0	8.465611733557331	0.0
29	0.0012352846713525132	0.0	0.0	8.646786818689034	0.0
30	0.0012352846713525132	0.0	0.0	8.909284811351442	0.0
31	0.0012352846713525132	0.0	0.0	9.10425390864658	0.0
32	0.0012352846713525132	0.0	0.0	9.312605256548038	0.0
33	0.0012352846713525132	0.0	0.0	9.506133188393266	0.0
34	0.0012352846713525132	0.0	0.0	9.705425782038137	0.0
35	0.0012352846713525132	0.0	0.0	9.920365314853475	0.0
36	0.0012352846713525132	0.0	0.0	10.135304847668811	0.0
37	0.0012352846713525132	0.0	0.0	10.368773650554436	0.0
38	0.0012352846713525132	0.0	0.0	10.662153760000658	0.0
39	0.0012352846713525132	0.0	0.0	11.192296764789445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	35	1.2099918E-7	45.000004	31
TTTCGCG	20	7.029261E-4	45.0	1
GTAGCCG	20	7.029261E-4	45.0	43
TGCGATG	25	3.8874674E-5	45.0	1
TAGGACG	25	3.8874674E-5	45.0	1
CGTTTTT	19100	0.0	44.458115	1
CGAATAT	55	6.002665E-11	40.909092	14
GCTACGA	55	6.002665E-11	40.909092	10
GTTTTTT	21100	0.0	40.382706	2
ACAACGA	95	0.0	40.263157	13
AGCGACT	95	0.0	40.263157	19
TCACGAC	40	3.452733E-7	39.375	25
CTCACGA	40	3.452733E-7	39.375	24
ATAGGGT	35	6.24084E-6	38.57143	4
AACGAGC	100	0.0	38.25	15
CACAACG	100	0.0	38.25	12
ATAGGGA	150	0.0	37.500004	4
CGATTGA	30	1.13895156E-4	37.499996	10
ACGGGTC	30	1.13895156E-4	37.499996	5
CTACGAA	60	1.546141E-10	37.499996	11
>>END_MODULE