FastQCFastQC Report
Sat 14 Jan 2017
SRR2935662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences749197
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT587307.839059686571089No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11440.15269682072939428No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA9470.1264020010758185No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA8070.1077153272103332No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA7560.1009080388736207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC207.0317107E-445.00000429
TCTACGG207.0317107E-445.0000042
GTATGCG207.0317107E-445.0000041
CGGTCTA207.0317107E-445.00000431
GGTACGA253.8895007E-545.08
AATACGG253.8895007E-545.02
CGTTTTT286500.044.7172781
GTTTTTT317150.040.487942
TAGGGCG1500.037.5000045
AACGTCG301.1395426E-437.4999961
CGCTACG301.1395426E-437.4999961
GGACGTA301.1395426E-437.4999969
ATACGGA301.1395426E-437.4999964
TAATGCG301.1395426E-437.4999961
TGCCCGA250.002106641236.017
CGAACGT250.002106641236.038
CTACTCA250.002106641236.029
GGATACG250.002106641236.01
ATGTACG250.002106641236.01
ACTACGG250.002106641236.02
Produced by FastQC (version 0.11.5)