FastQCFastQC Report
Sat 14 Jan 2017
SRR2935658.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935658.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417170
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272186.524438478318191No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC28790.690126327396505No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC28160.6750245703190546No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG23660.5671548769086943No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC16920.40559004722295466TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG15270.36603782630582254TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC10710.2567298703166575TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT9850.23611477335378864No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC9520.22820432917036218No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT8860.21238344080350938TruSeq Adapter, Index 20 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT8230.1972816837260589No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT8220.19704197329625814No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT6510.1560514898003212No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACT6220.149099887336098No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6050.14502481002948436No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT5600.13423784068844835No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC4710.11290361243617711No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA4610.1105065081381691No Hit
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC4360.10451374739314909TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4260.10211664309514107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA302.1621636E-645.00000413
CGGCGAA302.1621636E-645.00000431
TCACGAC207.0281135E-445.025
CTTAAGG253.8865182E-545.02
AACGTTG207.0281135E-445.01
GTGTTCG207.0281135E-445.01
CTCACGA207.0281135E-445.024
ACTGCGG207.0281135E-445.02
CGATTGT207.0281135E-445.010
CACGACG207.0281135E-445.026
AGGCGAC207.0281135E-445.09
AACGGGC253.8865182E-545.04
TACGCGG207.0281135E-445.02
TGTTCGG253.8865182E-545.02
CGGTCTA207.0281135E-445.031
AAGGCGA207.0281135E-445.08
CGTTTCT1350.045.01
CGCCCAC207.0281135E-445.034
TGCGTAG207.0281135E-445.01
CGTTTTT145700.044.598491
Produced by FastQC (version 0.11.5)