##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935658.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417170
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09862406213294	33.0	31.0	34.0	30.0	34.0
2	32.310170913536446	34.0	31.0	34.0	30.0	34.0
3	32.18328738883429	33.0	31.0	34.0	30.0	34.0
4	35.870045784692095	37.0	35.0	37.0	35.0	37.0
5	35.90937267780521	37.0	35.0	37.0	35.0	37.0
6	35.92218759738236	37.0	35.0	37.0	35.0	37.0
7	36.12121197593307	37.0	35.0	37.0	35.0	37.0
8	35.829208715871225	37.0	35.0	37.0	35.0	37.0
9	37.67327228707721	39.0	38.0	39.0	35.0	39.0
10	37.40999832202699	39.0	37.0	39.0	34.0	39.0
11	37.442114725411706	39.0	37.0	39.0	34.0	39.0
12	37.456528513555625	39.0	37.0	39.0	35.0	39.0
13	37.44158017115325	39.0	37.0	39.0	35.0	39.0
14	38.80237313325503	40.0	38.0	41.0	35.0	41.0
15	38.875441666466905	40.0	38.0	41.0	35.0	41.0
16	38.96291439940552	41.0	38.0	41.0	35.0	41.0
17	38.898770285495125	40.0	38.0	41.0	35.0	41.0
18	38.305371910731836	39.0	38.0	40.0	35.0	41.0
19	37.62415082580243	37.0	37.0	40.0	35.0	41.0
20	36.81225879138001	37.0	35.0	40.0	34.0	41.0
21	36.82091713210442	37.0	35.0	40.0	33.0	41.0
22	36.8339382026512	37.0	35.0	40.0	33.0	41.0
23	36.80322170817652	37.0	35.0	40.0	33.0	41.0
24	36.65515017858427	36.0	35.0	40.0	33.0	41.0
25	36.60066879209914	36.0	35.0	40.0	33.0	41.0
26	36.445156650765874	36.0	35.0	40.0	33.0	41.0
27	36.33004290816694	36.0	35.0	40.0	33.0	41.0
28	36.20401514969917	36.0	35.0	40.0	33.0	41.0
29	36.00098520986648	36.0	35.0	40.0	32.0	41.0
30	35.516707816957116	36.0	35.0	40.0	30.0	41.0
31	34.94642232183522	36.0	35.0	40.0	25.0	41.0
32	33.87318838842678	36.0	34.0	40.0	18.0	41.0
33	32.67214085384855	36.0	33.0	40.0	12.0	41.0
34	31.633856701105064	36.0	29.0	40.0	8.0	41.0
35	30.98085432797181	36.0	23.0	40.0	7.0	41.0
36	30.616755759043077	36.0	22.0	40.0	7.0	41.0
37	30.44086823117674	35.0	22.0	40.0	7.0	41.0
38	30.341462713042645	35.0	21.0	40.0	7.0	41.0
39	30.22774887935374	35.0	21.0	40.0	7.0	41.0
40	30.16130114821296	35.0	21.0	40.0	7.0	41.0
41	29.97718675839586	35.0	21.0	40.0	7.0	41.0
42	29.851861351487404	35.0	20.0	40.0	7.0	41.0
43	29.749394731164752	35.0	18.0	40.0	7.0	41.0
44	29.64937315722607	35.0	18.0	40.0	7.0	41.0
45	29.57914279550303	35.0	20.0	40.0	7.0	41.0
46	29.498719946304863	35.0	20.0	40.0	7.0	41.0
47	29.4286406021526	35.0	20.0	40.0	7.0	41.0
48	29.36795311263993	35.0	20.0	40.0	7.0	41.0
49	29.33159862885634	35.0	18.0	40.0	7.0	41.0
50	29.177452836972936	35.0	18.0	40.0	7.0	41.0
51	28.526480811180093	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	2.0
10	3.0
11	3.0
12	2.0
13	2.0
14	14.0
15	31.0
16	50.0
17	110.0
18	230.0
19	479.0
20	845.0
21	1402.0
22	2322.0
23	3956.0
24	7441.0
25	14791.0
26	23508.0
27	26177.0
28	21934.0
29	16454.0
30	12970.0
31	11658.0
32	11799.0
33	13115.0
34	18564.0
35	26054.0
36	27595.0
37	30412.0
38	53308.0
39	91851.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.3244240956924	13.66109739434763	15.20651053527339	42.807967974686576
2	55.638229019344635	17.876645012824508	15.798355586451565	10.686770381379294
3	18.385070834432007	17.74432485557447	52.842006855718296	11.028597454275236
4	16.42807488553827	15.632715679459213	56.10087014886018	11.83833928614234
5	17.36246614090179	17.664261572020997	52.3043363616751	12.668935925402113
6	14.88314116547211	23.35067238775559	51.90066399789055	9.865522448881752
7	48.27839969317065	3.197976843972481	44.447347604094254	4.076275858762615
8	47.87712443368411	4.930124409713066	42.20413740201836	4.9886137545844615
9	44.51158999928087	3.687225831195915	43.44583742838651	8.355346741136707
10	24.424814823692977	13.024426492796701	49.733202291631706	12.81755639187861
11	18.191145096723158	15.41314092576168	51.93518229978187	14.460531677733298
12	15.534913824100485	13.612675887527868	54.96919720977059	15.883213078601049
13	14.741232591030034	15.447659227652997	57.68967087757988	12.121437303737086
14	12.413884028094062	18.933048876956637	54.36632547882159	14.286741616127719
15	11.008461778171968	17.25411702663183	58.232135580219094	13.505285614977108
16	12.204856533307765	16.7965098161421	54.80379701320805	16.194836637342092
17	12.21875973823621	17.871850804228494	53.7730421650646	16.136347292470695
18	12.72718555984371	17.3948270489249	54.612028669367405	15.265958721863987
19	12.473571925114461	19.520579140398397	52.75762878442841	15.248220150058728
20	13.573842797900138	18.398974039360454	54.026176378934245	14.001006783805165
21	12.421315051417887	20.64721816046216	53.410839705635595	13.52062708248436
22	11.704101445453892	16.656279214708633	53.34587817915958	18.2937411606779
23	11.921279094853416	19.033727257472975	52.99158616391399	16.053407483759617
24	13.407963180477983	16.9451302826186	53.356904858930406	16.29000167797301
25	11.99079511949565	20.090850252894505	51.30210705467795	16.616247572931897
26	11.406381091641297	17.891507059472158	53.81690917371815	16.885202675168394
27	13.722702974806433	17.77620634273797	52.20701392717597	16.294076755279622
28	11.075340988086392	17.404175755687127	53.58127382122396	17.939209435002518
29	12.30865114941151	16.130594242155478	52.02291631708895	19.537838291344055
30	12.294987654912866	18.03940839465925	52.25016180454012	17.41544214588777
31	11.919601121844812	18.444519021022604	49.02078289426373	20.615096962868854
32	12.79981782007335	21.62355874104082	46.81089244193014	18.765730996955675
33	12.510727041733585	20.026128436848285	45.37215044226574	22.090994079152384
34	12.352038737205456	20.93175444063571	43.98686386844692	22.729342953711917
35	13.113119351822997	22.965937147925306	41.9152863341084	22.0056571661433
36	13.306325958242443	23.116954718699812	40.06448210561642	23.51223721744133
37	13.415394203801808	24.377591869022222	42.31320564757773	19.893808279598247
38	13.25430879497567	25.149219742550997	38.70316657477767	22.893304887695663
39	15.096483447994821	23.360021094517823	40.064002684756815	21.47949277273054
40	14.988613754584462	22.79358534889853	41.65208428218712	20.56571661432989
41	13.966009061054246	23.685787568617112	39.752379126015775	22.59582424431287
42	14.722774887935374	23.58654745067958	40.04482585037275	21.6458518110123
43	15.678500371551166	22.072776086487522	39.32521514011075	22.923508401850565
44	15.555049500203754	22.061509696286883	38.755183738044444	23.62825706546492
45	15.212743006448209	20.924802838171487	39.39664884819138	24.465805307188916
46	16.979648584509913	22.115684253421865	39.29740873025385	21.60725843181437
47	13.418510439389216	21.536064434163528	43.05606826953041	21.989356856916846
48	14.1616127717717	21.08061461754201	41.01493395977659	23.7428386509097
49	14.584222259510513	19.645468274324614	42.52750677181964	23.24280269434523
50	14.34403240885011	18.908838123546758	42.317280724884334	24.429848742718796
51	14.242874607474171	19.15885610182899	39.53136610973944	27.066903180957404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	207.5
2	299.0
3	21162.5
4	42026.0
5	28160.0
6	14294.0
7	13848.0
8	13402.0
9	13090.0
10	12778.0
11	12519.0
12	12260.0
13	11613.5
14	10967.0
15	10349.0
16	9731.0
17	8934.5
18	8138.0
19	7621.0
20	7104.0
21	6523.0
22	5942.0
23	5541.5
24	5141.0
25	4752.0
26	4201.5
27	4040.0
28	4055.5
29	4071.0
30	4205.5
31	4340.0
32	4756.0
33	5172.0
34	5651.0
35	6130.0
36	6697.5
37	7265.0
38	8068.0
39	8871.0
40	9783.0
41	10695.0
42	12540.5
43	14386.0
44	17022.0
45	19658.0
46	29734.0
47	39810.0
48	39268.0
49	38726.0
50	37518.5
51	36311.0
52	29254.0
53	22197.0
54	18089.0
55	13981.0
56	11740.5
57	9500.0
58	8100.0
59	6700.0
60	6124.5
61	5549.0
62	4860.0
63	4171.0
64	3463.0
65	2755.0
66	2358.5
67	1962.0
68	1802.5
69	1643.0
70	1282.5
71	922.0
72	805.5
73	689.0
74	586.5
75	403.0
76	322.0
77	218.5
78	115.0
79	93.5
80	72.0
81	47.5
82	23.0
83	19.0
84	15.0
85	16.0
86	17.0
87	16.0
88	15.0
89	8.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	417170.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.704635803230026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1574901059134	44.35489866146101
2	7.741801854376552	8.16057694391374
3	2.4540869154997114	3.8802527133265334
4	1.2510046035361206	2.6373496807014165
5	0.7347468988179086	1.9362283854875288
6	0.5417913102053171	1.713294821143564
7	0.441208739927724	1.6277622165764911
8	0.3647837004263534	1.5380633662340417
9	0.3053065822791243	1.4481965004615114
>10	1.9000141450364587	16.130912733145554
>50	0.07337201292347048	2.5776273289769542
>100	0.0270559297655148	2.762278738196419
>500	0.004127175726942936	1.6691133927714121
>1k	0.002751450484628624	2.9851172189573867
>5k	0.0	0.0
>10k+	4.5857508077143736E-4	6.578327298646437
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27218	6.524438478318191	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	2879	0.690126327396505	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	2816	0.6750245703190546	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	2366	0.5671548769086943	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1692	0.40559004722295466	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	1527	0.36603782630582254	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1071	0.2567298703166575	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	985	0.23611477335378864	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	952	0.22820432917036218	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	886	0.21238344080350938	TruSeq Adapter, Index 20 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	823	0.1972816837260589	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	822	0.19704197329625814	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	651	0.1560514898003212	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACT	622	0.149099887336098	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	605	0.14502481002948436	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	560	0.13423784068844835	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	471	0.11290361243617711	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	461	0.1105065081381691	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	436	0.10451374739314909	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	426	0.10211664309514107	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.191312894024019E-4	0.0	0.0	0.2226909892849438	0.0
2	7.191312894024019E-4	0.0	0.0	1.0964355059088622	0.0
3	7.191312894024019E-4	0.0	0.0	1.807176930268236	0.0
4	7.191312894024019E-4	0.0	0.0	2.6744492652875325	0.0
5	7.191312894024019E-4	0.0	0.0	4.977587074813625	0.0
6	7.191312894024019E-4	0.0	0.0	6.415609943188628	0.0
7	7.191312894024019E-4	0.0	0.0	7.719394970875182	0.0
8	7.191312894024019E-4	0.0	0.0	9.549104681544694	0.0
9	7.191312894024019E-4	0.0	0.0	10.276386125560323	0.0
10	7.191312894024019E-4	0.0	0.0	12.315602751875733	0.0
11	7.191312894024019E-4	0.0	0.0	14.147949277273055	0.0
12	7.191312894024019E-4	0.0	0.0	16.11405422249922	0.0
13	7.191312894024019E-4	0.0	0.0	16.798427499580505	0.0
14	7.191312894024019E-4	0.0	0.0	17.076012177289833	4.7942085960160126E-4
15	7.191312894024019E-4	0.0	0.0	17.547282882278207	4.7942085960160126E-4
16	7.191312894024019E-4	0.0	0.0	18.438046839417982	4.7942085960160126E-4
17	7.191312894024019E-4	0.0	0.0	19.456576455641585	4.7942085960160126E-4
18	7.191312894024019E-4	0.0	0.0	20.52712323513196	4.7942085960160126E-4
19	7.191312894024019E-4	0.0	0.0	21.314571997027592	4.7942085960160126E-4
20	9.588417192032025E-4	0.0	0.0	21.93086751204545	4.7942085960160126E-4
21	9.588417192032025E-4	0.0	0.0	22.746362394227774	4.7942085960160126E-4
22	9.588417192032025E-4	0.0	0.0	23.582712083802765	4.7942085960160126E-4
23	9.588417192032025E-4	0.0	0.0	24.485701272862382	4.7942085960160126E-4
24	9.588417192032025E-4	0.0	0.0	25.122132463983508	4.7942085960160126E-4
25	9.588417192032025E-4	0.0	0.0	25.737229426852362	4.7942085960160126E-4
26	9.588417192032025E-4	0.0	0.0	26.26315410983532	4.7942085960160126E-4
27	9.588417192032025E-4	0.0	0.0	26.813529256657958	4.7942085960160126E-4
28	9.588417192032025E-4	0.0	0.0	27.362945561761393	4.7942085960160126E-4
29	9.588417192032025E-4	0.0	0.0	27.9051705539708	4.7942085960160126E-4
30	9.588417192032025E-4	0.0	0.0	28.47927703334372	4.7942085960160126E-4
31	9.588417192032025E-4	0.0	0.0	29.059615983891458	4.7942085960160126E-4
32	9.588417192032025E-4	0.0	0.0	29.603518949109475	4.7942085960160126E-4
33	9.588417192032025E-4	0.0	0.0	30.12848479037323	4.7942085960160126E-4
34	9.588417192032025E-4	0.0	0.0	30.578900687968932	4.7942085960160126E-4
35	9.588417192032025E-4	0.0	0.0	31.05113023467651	4.7942085960160126E-4
36	0.0011985521490040032	0.0	0.0	31.518086151928472	4.7942085960160126E-4
37	0.0011985521490040032	0.0	0.0	32.00397919313469	4.7942085960160126E-4
38	0.0011985521490040032	0.0	0.0	32.437615360644344	4.7942085960160126E-4
39	0.0011985521490040032	0.0	0.0	32.88635328523144	4.7942085960160126E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	30	2.1621636E-6	45.000004	13
CGGCGAA	30	2.1621636E-6	45.000004	31
TCACGAC	20	7.0281135E-4	45.0	25
CTTAAGG	25	3.8865182E-5	45.0	2
AACGTTG	20	7.0281135E-4	45.0	1
GTGTTCG	20	7.0281135E-4	45.0	1
CTCACGA	20	7.0281135E-4	45.0	24
ACTGCGG	20	7.0281135E-4	45.0	2
CGATTGT	20	7.0281135E-4	45.0	10
CACGACG	20	7.0281135E-4	45.0	26
AGGCGAC	20	7.0281135E-4	45.0	9
AACGGGC	25	3.8865182E-5	45.0	4
TACGCGG	20	7.0281135E-4	45.0	2
TGTTCGG	25	3.8865182E-5	45.0	2
CGGTCTA	20	7.0281135E-4	45.0	31
AAGGCGA	20	7.0281135E-4	45.0	8
CGTTTCT	135	0.0	45.0	1
CGCCCAC	20	7.0281135E-4	45.0	34
TGCGTAG	20	7.0281135E-4	45.0	1
CGTTTTT	14570	0.0	44.59849	1
>>END_MODULE