##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935657.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468054
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38685493554163	33.0	31.0	34.0	30.0	34.0
2	32.58142436556466	34.0	31.0	34.0	31.0	34.0
3	32.45269349263119	34.0	31.0	34.0	30.0	34.0
4	36.06860105885218	37.0	35.0	37.0	35.0	37.0
5	36.09463010678255	37.0	35.0	37.0	35.0	37.0
6	36.118174398680495	37.0	36.0	37.0	35.0	37.0
7	36.28600332440274	37.0	37.0	37.0	35.0	37.0
8	36.034218273959844	37.0	37.0	37.0	35.0	37.0
9	37.881043640263734	39.0	38.0	39.0	35.0	39.0
10	37.7243971849402	39.0	38.0	39.0	35.0	39.0
11	37.638481884568876	39.0	37.0	39.0	35.0	39.0
12	37.66054130506309	39.0	37.0	39.0	35.0	39.0
13	37.68236998295068	39.0	38.0	39.0	35.0	39.0
14	39.11747148833254	41.0	39.0	41.0	35.0	41.0
15	39.1939199323155	41.0	39.0	41.0	35.0	41.0
16	39.26367684070642	41.0	39.0	41.0	35.0	41.0
17	39.20138274643524	41.0	39.0	41.0	35.0	41.0
18	38.58389630256338	39.0	39.0	41.0	35.0	41.0
19	37.925867528105734	38.0	37.0	41.0	35.0	41.0
20	37.108970332483004	37.0	35.0	41.0	34.0	41.0
21	37.127425468001555	37.0	35.0	41.0	34.0	41.0
22	37.12985040187671	37.0	35.0	41.0	34.0	41.0
23	37.10044353856606	37.0	35.0	41.0	34.0	41.0
24	36.96714268011811	37.0	35.0	41.0	34.0	41.0
25	36.931943750080116	37.0	35.0	41.0	34.0	41.0
26	36.75350066445325	37.0	35.0	41.0	33.0	41.0
27	36.657229721356934	37.0	35.0	40.0	33.0	41.0
28	36.528409115187564	37.0	35.0	40.0	33.0	41.0
29	36.306763749481895	37.0	35.0	40.0	33.0	41.0
30	35.814350480927416	37.0	35.0	40.0	31.0	41.0
31	35.16243211253403	37.0	35.0	40.0	25.0	41.0
32	34.10403500450803	37.0	34.0	40.0	18.0	41.0
33	32.951153072081425	37.0	33.0	40.0	12.0	41.0
34	31.977444910202667	37.0	30.0	41.0	8.0	41.0
35	31.42668794626261	37.0	25.0	41.0	7.0	41.0
36	31.099020625825226	37.0	23.0	41.0	7.0	41.0
37	30.889931503629924	37.0	23.0	40.0	7.0	41.0
38	30.75005448089323	36.0	23.0	40.0	7.0	41.0
39	30.641876791994086	36.0	22.0	40.0	7.0	41.0
40	30.53383797596004	36.0	22.0	40.0	7.0	41.0
41	30.402139496724736	36.0	21.0	40.0	7.0	41.0
42	30.261497604977205	36.0	21.0	40.0	7.0	41.0
43	30.112566071436202	36.0	20.0	40.0	7.0	41.0
44	30.01150721925248	36.0	18.0	40.0	7.0	41.0
45	29.94421370183782	35.0	20.0	40.0	7.0	41.0
46	29.864075512654523	35.0	20.0	40.0	7.0	41.0
47	29.815004251646176	35.0	20.0	40.0	7.0	41.0
48	29.795974396116687	35.0	20.0	40.0	7.0	41.0
49	29.766907237199128	35.0	20.0	40.0	7.0	41.0
50	29.63057254077521	35.0	20.0	40.0	7.0	41.0
51	29.072173296243594	35.0	19.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	6.0
11	4.0
12	5.0
13	3.0
14	8.0
15	19.0
16	38.0
17	103.0
18	189.0
19	442.0
20	788.0
21	1401.0
22	2225.0
23	3780.0
24	7448.0
25	14728.0
26	24570.0
27	28628.0
28	25973.0
29	19828.0
30	14574.0
31	12427.0
32	11608.0
33	12315.0
34	17664.0
35	26717.0
36	27816.0
37	29584.0
38	58913.0
39	126108.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.45131971951954	13.562110354788123	15.709512150307441	43.27705777538489
2	55.40899127023805	17.65245035829199	15.967388378264047	10.971169993205912
3	19.923555829028274	17.942587821063384	51.48743521046717	10.646421139441175
4	17.658859875142614	16.53612617347571	53.886517367654164	11.918496583727519
5	18.55405572861251	18.434625064629294	50.375597687446316	12.635721519311874
6	16.525443645391345	23.10652189704607	50.2157870673042	10.152247390258388
7	52.238630585359815	3.070158571446884	41.635580509941164	3.055630333252146
8	52.668922816598084	4.5353741234985705	39.57556179415196	3.2201412657513875
9	48.89478564439146	4.993868228879574	40.32825272297641	5.7830934037525585
10	23.914761971909222	23.686369521465473	44.65189913984284	7.746969366782465
11	17.34009323710512	16.070367948997337	54.64540416276754	11.944134651129998
12	16.34362701739543	14.59489716998466	55.42052840056917	13.640947412050746
13	15.073260777602584	15.38390869429596	57.136569712041776	12.406260816059685
14	12.342165647553488	19.747721416759607	53.75875433176513	14.151358603921771
15	11.154909476257014	18.06265943673166	57.90101142175903	12.8814196652523
16	12.914749152875524	17.65586876727899	55.20281847820978	14.226563601635709
17	12.950642447238994	18.019074722147444	54.391373644921316	14.63890918569225
18	13.310002691997077	17.451618830305904	54.86140488063344	14.376973597063586
19	13.945613113016876	18.35899276579198	53.52566156896427	14.169732552226879
20	16.664316510488106	17.754788977340223	54.179004986604106	11.401889525567563
21	14.784191567639631	18.898887735175858	53.626290983519	12.690629713665516
22	13.36576548859747	16.46583513868058	54.595623581894394	15.572775790827553
23	13.653339144628612	18.75467360603691	54.05423305857872	13.537754190755768
24	13.952022629867495	16.726061522815744	54.382827622453824	14.939088224862942
25	12.67930623389609	20.156007640144086	52.60354574472176	14.561140381238063
26	13.014523965183505	18.666435924060043	53.81729458566746	14.501745525088985
27	14.916654915885774	18.927730561003646	52.94325013780461	13.21236438530597
28	11.821499228721473	19.757122041473846	54.3039905651912	14.117388164613484
29	14.435513850965915	17.232840655138084	53.98800138445564	14.343644109440365
30	15.755019719946844	18.449794254509094	52.023484469740666	13.77170155580339
31	14.821366765373226	20.45618667931478	48.99926076905656	15.723185786255431
32	17.427903617958613	21.25224867216176	47.49024685185898	13.829600858020655
33	17.32556499891038	21.728048473039436	44.89567443072808	16.050712097322105
34	15.860135796297008	23.006747084738084	43.56334952804591	17.569767590918996
35	17.575322505522866	24.2068222897358	41.05594653608344	17.16190866865789
36	17.47917975276357	25.94871531917257	40.816871557555324	15.755233370508531
37	18.47393676797976	25.6380674024792	39.73473146260901	16.15326436693202
38	18.170766620945447	26.563815286270387	37.83046400628987	17.434954086494294
39	19.7528490302401	23.827806193302482	38.97648561918069	17.442859157276725
40	19.010840629500013	23.95172351908113	39.74990065248882	17.287535198930037
41	16.664743811611483	25.567990018245755	38.724591606951336	19.04267456319143
42	18.15687933443577	25.7459609361313	38.85427749789554	17.242882231537386
43	19.23346451477821	25.280202711652926	38.10073196682434	17.385600806744524
44	18.83137415768266	24.937720861268144	37.50528785140176	18.725617129647436
45	18.51901703649579	23.834643011276476	36.98675793818662	20.659582014041113
46	19.34926311921274	25.150516820708724	37.30552457622411	18.194695483854428
47	16.673289834078975	24.61938152435403	40.493831908284086	18.21349673328291
48	17.151012490011837	23.57527122938806	39.27089609318583	20.002820187414272
49	17.634503711110256	22.37690522888385	41.47662449204579	18.511966567960105
50	16.969409512577606	22.299777376114722	40.054139052331564	20.6766740589761
51	16.383152371307585	21.847265486460966	38.95042025065484	22.819161891576613
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	93.0
1	201.5
2	310.0
3	23856.0
4	47402.0
5	31814.0
6	16226.0
7	15661.5
8	15097.0
9	14898.5
10	14700.0
11	14047.0
12	13394.0
13	12824.0
14	12254.0
15	11399.0
16	10544.0
17	9857.0
18	9170.0
19	8536.5
20	7903.0
21	7103.0
22	6303.0
23	5772.5
24	5242.0
25	5130.0
26	5248.0
27	5478.0
28	5795.0
29	6112.0
30	6658.0
31	7204.0
32	7983.5
33	8763.0
34	10031.5
35	11300.0
36	11873.5
37	12447.0
38	13047.5
39	13648.0
40	14878.5
41	16109.0
42	17728.0
43	19347.0
44	20681.5
45	22016.0
46	28514.0
47	35012.0
48	33780.5
49	32549.0
50	31242.0
51	29935.0
52	25726.5
53	21518.0
54	18874.5
55	16231.0
56	14390.0
57	12549.0
58	11410.5
59	10272.0
60	9589.5
61	8907.0
62	7911.5
63	6916.0
64	5377.0
65	3838.0
66	2951.5
67	2065.0
68	1447.5
69	830.0
70	622.5
71	415.0
72	435.0
73	455.0
74	341.5
75	187.5
76	147.0
77	104.0
78	61.0
79	42.5
80	24.0
81	22.0
82	20.0
83	11.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.588415711361534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12158149564004	34.9237280168369
2	7.885062843649328	6.873947942783949
3	2.742269776087352	3.585935849783934
4	1.4792751851584531	2.579170468887519
5	1.0312961895891644	2.2476283516677804
6	0.8082642245209668	2.1138574213824692
7	0.6763531674757178	2.0636814122139397
8	0.5828679900792306	2.0325033805135404
9	0.5196699591805192	2.0386431192120074
>10	4.023399296548573	28.105981413736153
>50	0.09586393605210759	2.8808351115413666
>100	0.029154496015844175	2.438209604683793
>500	0.002470720001342727	0.6653382923028478
>1k	0.0019765760010741814	1.6572071288371997
>5k	0.0	0.0
>10k+	4.941440002685453E-4	5.793332485616606
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26897	5.746559157704026	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC	2255	0.48178201660492165	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC	2175	0.46468997166993553	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG	1889	0.40358591102736013	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	1375	0.2937695223200742	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	756	0.16151982463561898	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT	695	0.14848714037269203	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	603	0.128831288697458	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGCATT	525	0.11216654488584651	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	510	0.1089617864605366	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	500	0.10682528084366334	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCT	474	0.10127036623979284	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15575125947006116	0.0
2	0.0	0.0	0.0	0.79200263217492	0.0
3	0.0	0.0	0.0	1.0932499241540505	0.0
4	0.0	0.0	0.0	1.541488802574062	0.0
5	0.0	0.0	0.0	2.936626970392305	0.0
6	0.0	0.0	0.0	3.4508838723737005	0.0
7	0.0	0.0	0.0	4.080939378789627	0.0
8	0.0	0.0	0.0	5.000064095168506	0.0
9	0.0	0.0	0.0	5.306011699504758	0.0
10	0.0	0.0	0.0	6.368709593337521	0.0
11	0.0	0.0	0.0	7.332914578232426	0.0
12	0.0	0.0	0.0	8.677631213492461	0.0
13	0.0	0.0	0.0	9.016908305451935	0.0
14	0.0	0.0	0.0	9.158558627850633	0.0
15	0.0	0.0	0.0	9.459805919829764	0.0
16	0.0	0.0	0.0	9.949706657778803	0.0
17	0.0	0.0	0.0	10.595999606882966	0.0
18	0.0	0.0	0.0	11.212167826789216	0.0
19	0.0	0.0	0.0	11.759113264708773	0.0
20	0.0	0.0	0.0	12.170390595956876	0.0
21	0.0	0.0	0.0	12.623543437295696	0.0
22	0.0	0.0	0.0	13.183094258354805	0.0
23	0.0	0.0	0.0	13.668081033385036	0.0
24	0.0	0.0	0.0	14.034491746678802	0.0
25	0.0	0.0	0.0	14.350480927414358	0.0
26	0.0	0.0	0.0	14.64531870254287	0.0
27	0.0	0.0	0.0	14.96323073833361	0.0
28	0.0	0.0	0.0	15.277938015699043	0.0
29	0.0	0.0	0.0	15.631316044729882	0.0
30	0.0	0.0	0.0	16.02635593328975	0.0
31	0.0	0.0	0.0	16.397680609502324	0.0
32	0.0	0.0	0.0	16.731402786857927	0.0
33	0.0	0.0	0.0	17.073457336119336	0.0
34	0.0	0.0	0.0	17.382182397757525	0.0
35	0.0	0.0	0.0	17.734492173979927	0.0
36	2.1365056168732668E-4	0.0	0.0	18.090006708627637	0.0
37	2.1365056168732668E-4	0.0	0.0	18.42864284890205	0.0
38	2.1365056168732668E-4	0.0	0.0	18.763433279066092	0.0
39	2.1365056168732668E-4	0.0	0.0	19.08882308451589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGCG	20	7.0289953E-4	45.000004	1
TAAACGA	20	7.0289953E-4	45.000004	27
TCGTGAG	20	7.0289953E-4	45.000004	1
AATTGCG	20	7.0289953E-4	45.000004	1
CATAGCC	20	7.0289953E-4	45.000004	10
GTTCACG	20	7.0289953E-4	45.000004	1
GTATACG	20	7.0289953E-4	45.000004	1
TGCTACG	20	7.0289953E-4	45.000004	1
CGGTCCT	20	7.0289953E-4	45.000004	38
CCAACGG	20	7.0289953E-4	45.000004	2
TGCGTAA	20	7.0289953E-4	45.000004	11
TGGCGTT	25	3.8872502E-5	45.0	35
TTCGTAG	25	3.8872502E-5	45.0	1
TCGGCGT	25	3.8872502E-5	45.0	4
ACAACGA	60	0.0	44.999996	13
AGCGTTG	30	2.1627347E-6	44.999996	1
ACGCCGG	30	2.1627347E-6	44.999996	27
TATGACG	30	2.1627347E-6	44.999996	1
GCTACGA	30	2.1627347E-6	44.999996	10
CGGTAGT	30	2.1627347E-6	44.999996	12
>>END_MODULE