##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935656.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543539
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40708946368154	34.0	31.0	34.0	30.0	34.0
2	32.61250986589739	34.0	31.0	34.0	31.0	34.0
3	32.46496203584287	34.0	31.0	34.0	31.0	34.0
4	36.06440200243221	37.0	35.0	37.0	35.0	37.0
5	36.1048241248558	37.0	35.0	37.0	35.0	37.0
6	36.12803681060605	37.0	36.0	37.0	35.0	37.0
7	36.25623368332355	37.0	37.0	37.0	35.0	37.0
8	36.01575967869831	37.0	37.0	37.0	35.0	37.0
9	37.82963136039916	39.0	38.0	39.0	35.0	39.0
10	37.62481809033022	39.0	38.0	39.0	35.0	39.0
11	37.6464154366108	39.0	37.0	39.0	35.0	39.0
12	37.61252642404685	39.0	37.0	39.0	35.0	39.0
13	37.641194100147366	39.0	38.0	39.0	35.0	39.0
14	39.07011272420194	41.0	39.0	41.0	35.0	41.0
15	39.167095645390674	41.0	39.0	41.0	35.0	41.0
16	39.199707840651726	41.0	39.0	41.0	35.0	41.0
17	39.15881841045445	41.0	39.0	41.0	35.0	41.0
18	38.50099624865925	39.0	39.0	41.0	35.0	41.0
19	37.80373809423059	37.0	37.0	41.0	35.0	41.0
20	36.99986017562677	37.0	35.0	41.0	34.0	41.0
21	36.96724798036571	36.0	35.0	40.0	34.0	41.0
22	36.957815354555976	36.0	35.0	40.0	34.0	41.0
23	36.954424613505196	36.0	35.0	40.0	34.0	41.0
24	36.790596442941535	36.0	35.0	40.0	34.0	41.0
25	36.68692586916486	36.0	35.0	40.0	33.0	41.0
26	36.52901447734201	35.0	35.0	40.0	33.0	41.0
27	36.444144762381356	35.0	35.0	40.0	33.0	41.0
28	36.28679082825703	35.0	35.0	40.0	33.0	41.0
29	36.00974355106074	35.0	35.0	40.0	33.0	41.0
30	35.467846833437896	35.0	35.0	40.0	31.0	41.0
31	34.776555132198425	35.0	35.0	40.0	23.0	41.0
32	33.70589046968111	35.0	33.0	40.0	17.0	41.0
33	32.46868945926603	35.0	32.0	40.0	12.0	41.0
34	31.447861147038207	35.0	27.0	40.0	7.0	41.0
35	30.827223069549746	35.0	23.0	40.0	7.0	41.0
36	30.441368512655025	35.0	21.0	40.0	7.0	41.0
37	30.249663777576217	35.0	21.0	40.0	7.0	41.0
38	30.091522411455294	35.0	20.0	40.0	7.0	41.0
39	29.958341535749966	35.0	20.0	40.0	7.0	41.0
40	29.844993643510403	35.0	19.0	40.0	7.0	41.0
41	29.670340122787877	35.0	18.0	40.0	7.0	41.0
42	29.53920141884943	35.0	18.0	40.0	7.0	41.0
43	29.43512609030815	35.0	18.0	40.0	7.0	41.0
44	29.331479433858473	35.0	18.0	40.0	7.0	41.0
45	29.273426561847447	35.0	18.0	40.0	7.0	41.0
46	29.222079740368216	35.0	18.0	40.0	7.0	41.0
47	29.203024989927126	35.0	18.0	40.0	7.0	41.0
48	29.128169275801735	35.0	18.0	40.0	7.0	41.0
49	29.08816110711467	35.0	18.0	40.0	7.0	41.0
50	28.976535262419073	35.0	18.0	40.0	7.0	41.0
51	28.38730431486977	35.0	18.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	14.0
10	17.0
11	7.0
12	4.0
13	13.0
14	18.0
15	27.0
16	66.0
17	113.0
18	317.0
19	569.0
20	967.0
21	1587.0
22	2492.0
23	4453.0
24	8906.0
25	17888.0
26	30786.0
27	36585.0
28	32136.0
29	24313.0
30	18260.0
31	15013.0
32	13969.0
33	15058.0
34	22903.0
35	31962.0
36	33479.0
37	36676.0
38	66150.0
39	128668.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.38007576273276	13.069715328614873	14.792682769773648	45.75752613887872
2	58.94038882214524	14.975374352162401	15.230921792180505	10.853315033511855
3	20.847630068863506	15.195965698873493	53.05323077092904	10.90317346133396
4	18.728554896704743	16.65676244022968	52.72611532935079	11.888567333714784
5	16.042455095218557	19.766750867923	51.82516801922217	12.36562601763627
6	16.836142392726188	21.803219272214136	52.09819350589379	9.262444829165892
7	50.05767755395657	4.504368591766184	42.13184334518774	3.306110509089504
8	50.457832832602634	3.9515103792000206	42.17636636929456	3.414290418902784
9	46.723786149659915	5.464189322201351	43.36708129499447	4.444943233144264
10	25.299196561792254	15.839893733476346	48.90081484493293	9.960094859798469
11	19.555910431450183	16.81627261337273	51.24544880864115	12.382368146535944
12	17.948482077643003	14.651018602161022	54.528009949608034	12.872489370587942
13	16.200125473977028	14.78201196234309	55.407431665437066	13.610430898242813
14	13.398486585139244	17.844901653791172	54.654587803267106	14.102023957802476
15	13.381560476801113	16.97762258090036	56.924526114961395	12.716290827337136
16	14.816048158457813	15.92783590505925	56.19688743586016	13.05922850062277
17	14.726266192490328	15.620222283957544	54.38340211098007	15.27010941257205
18	14.628389131230692	16.691718533536694	54.837279385655854	13.842612949576754
19	16.060853039064355	17.437387197606796	53.19379106191092	13.30796870141793
20	16.53791172298584	16.754823480927772	53.60535306574137	13.101911730345018
21	16.13610062939366	16.984797779000218	53.8117779956912	13.067323595914921
22	14.7557029026436	16.30554569221344	53.49662121761271	15.442130187530243
23	14.59012140803144	17.060229348767983	54.217452657490995	14.13219658570958
24	14.931771225247866	16.227722389745722	54.35470131858063	14.485805066425778
25	14.559580821247417	17.112295529851586	53.19452697966476	15.133596669236246
26	14.156849830462948	17.513186726251472	53.820241049860265	14.509722393425312
27	14.933794999070905	17.2640785665794	53.79098831914545	14.011138115204243
28	13.650722395265106	17.32221606913211	54.57566062416864	14.451400911434137
29	15.14224370284377	16.789963553673243	53.421005668406494	14.646787075076489
30	15.119246273036527	16.763654493973753	52.320808626427905	15.796290606561811
31	14.84419701254188	18.07597982849437	51.13174951567413	15.948073643289625
32	16.269301742837218	19.34433407722353	49.21468376694221	15.171680412997043
33	16.208588528146095	20.52088258616217	46.45738392277279	16.813144962918944
34	15.970335155343038	22.230419528313515	44.908461030395245	16.8907842859482
35	17.533608443920308	22.701038931888974	41.75137386645669	18.013978757734037
36	17.738561538362475	24.385922629286952	40.25838072337036	17.61713510898022
37	17.647123757448867	24.88873843459255	41.05887526010093	16.405262547857653
38	18.072116260286748	24.70144736624235	39.28402561729701	17.942410756173892
39	18.167233629969516	23.7778705851834	39.89722908567738	18.1576666991697
40	18.805090343103252	23.669874654808577	40.099238509104225	17.425796492983945
41	17.93965106459702	24.46429787007004	38.99738565217951	18.598665413153427
42	18.38598518229603	24.71782153626511	38.84762638927474	18.048566892164132
43	18.08113125277119	24.07518135773146	39.2919367331507	18.55175065634665
44	17.96393635047347	24.03286608688613	38.642121356517194	19.361076206123204
45	17.93339576368945	23.043608646297688	39.220736690467476	19.802258899545386
46	17.777749158754016	23.52471487786525	39.32339721712702	19.37413874625372
47	17.41880527432254	22.874531542354827	41.53262231413017	18.17404086919246
48	17.649515490148822	21.824376907636804	41.2187533921209	19.30735421009348
49	17.16399375205827	21.4047198085142	42.11270948358811	19.318576955839415
50	16.37104237230447	21.502228910896918	42.234135912970366	19.892592803828244
51	16.41427754034209	21.464329146574578	40.5404212025264	21.580972110556925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	310.0
2	511.0
3	29302.0
4	58093.0
5	39794.5
6	21496.0
7	20510.0
8	19524.0
9	18790.0
10	18056.0
11	17345.5
12	16635.0
13	15830.0
14	15025.0
15	14025.0
16	13025.0
17	12133.5
18	11242.0
19	10315.0
20	9388.0
21	8509.5
22	7631.0
23	6879.0
24	6127.0
25	5993.5
26	5610.5
27	5361.0
28	5556.0
29	5751.0
30	6061.5
31	6372.0
32	6831.0
33	7290.0
34	7924.0
35	8558.0
36	9502.5
37	10447.0
38	11301.5
39	12156.0
40	13844.5
41	15533.0
42	17086.5
43	18640.0
44	20607.5
45	22575.0
46	25982.5
47	29390.0
48	32027.5
49	34665.0
50	34012.5
51	33360.0
52	30017.0
53	26674.0
54	23453.5
55	20233.0
56	19059.5
57	17886.0
58	16950.0
59	16014.0
60	15110.5
61	14207.0
62	12997.5
63	11788.0
64	10157.5
65	8527.0
66	6846.0
67	5165.0
68	4340.5
69	3516.0
70	3107.0
71	2698.0
72	2215.0
73	1732.0
74	1368.5
75	830.5
76	656.0
77	490.5
78	325.0
79	235.5
80	146.0
81	124.5
82	103.0
83	72.0
84	41.0
85	21.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	543539.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.410319812805966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30924046157494	36.46868293283655
2	7.617830178243508	6.918562093473647
3	2.770883676826913	3.7748014178638147
4	1.5180963441492765	2.7574896197788075
5	1.087655076292891	2.469538243024103
6	0.8765497692894204	2.388264321316432
7	0.7100501303369745	2.25705224512086
8	0.610662857317231	2.218431651886189
9	0.5736882093999971	2.344622855552088
>10	3.8075163880563303	26.66113550893831
>50	0.07787176306993583	2.5266110568290907
>100	0.03710120648241531	2.6321410852468374
>500	0.0024462333944449653	0.7274166366979548
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.0770556574082757E-4	5.855250331435357
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31626	5.818533720671377	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	781	0.14368794143566516	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	745	0.13706468165117866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	643	0.11829877892846695	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	636	0.11701092285926123	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	603	0.11093960139014865	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.198971922897897E-4	0.0	0.0	0.09438145192893242	0.0
2	9.198971922897897E-4	0.0	0.0	0.46344420547559606	0.0
3	0.0011038766307477476	0.0	0.0	0.6886350381481365	0.0
4	0.0011038766307477476	0.0	0.0	0.957980936050587	0.0
5	0.0011038766307477476	0.0	0.0	1.7032816412437746	0.0
6	0.0011038766307477476	0.0	0.0	2.146304129050537	0.0
7	0.0011038766307477476	0.0	0.0	2.585463048649683	0.0
8	0.0011038766307477476	0.0	0.0	3.3044546941433826	0.0
9	0.0011038766307477476	0.0	0.0	3.569201106084384	0.0
10	0.0014718355076636636	0.0	0.0	4.168054178265037	0.0
11	0.0014718355076636636	0.0	0.0	4.983451049510706	0.0
12	0.0014718355076636636	0.0	0.0	5.667486601697394	0.0
13	0.0014718355076636636	0.0	0.0	5.948607183661154	0.0
14	0.0014718355076636636	0.0	0.0	6.074633099004855	0.0
15	0.0014718355076636636	0.0	0.0	6.27333089253945	0.0
16	0.0014718355076636636	0.0	0.0	6.710466038315558	0.0
17	0.0014718355076636636	0.0	0.0	7.219721123967186	0.0
18	0.0014718355076636636	0.0	0.0	7.771107501025686	0.0
19	0.0014718355076636636	0.0	0.0	8.107421914526833	0.0
20	0.0014718355076636636	0.0	0.0	8.437297047681952	0.0
21	0.0014718355076636636	0.0	0.0	8.863945365465955	0.0
22	0.0014718355076636636	0.0	0.0	9.304576120572765	0.0
23	0.0014718355076636636	0.0	0.0	9.744838916802658	0.0
24	0.0014718355076636636	0.0	0.0	10.108382287195584	0.0
25	0.0014718355076636636	0.0	0.0	10.420227435381822	0.0
26	0.0014718355076636636	0.0	0.0	10.717906166806797	0.0
27	0.0014718355076636636	0.0	0.0	11.017976630931727	0.0
28	0.0014718355076636636	0.0	0.0	11.318966992248946	0.0
29	0.0014718355076636636	0.0	0.0	11.626396633912194	0.0
30	0.0014718355076636636	0.0	0.0	11.961423191344135	0.0
31	0.0014718355076636636	0.0	0.0	12.301785152491357	0.0
32	0.0014718355076636636	0.0	0.0	12.625956923054279	0.0
33	0.0014718355076636636	0.0	0.0	12.947369002040332	0.0
34	0.0014718355076636636	0.0	0.0	13.250751096057504	0.0
35	0.0014718355076636636	0.0	0.0	13.568483586274398	0.0
36	0.0014718355076636636	0.0	0.0	13.90443004089863	0.0
37	0.0016558149461216214	0.0	0.0	14.249207508568842	0.0
38	0.0016558149461216214	0.0	0.0	14.575035094077887	0.0
39	0.0016558149461216214	0.0	0.0	14.887984118894872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGG	30	2.1633823E-6	45.000004	2
TATACGG	30	2.1633823E-6	45.000004	2
GTTGACG	20	7.0300006E-4	45.0	1
CAACGAC	20	7.0300006E-4	45.0	12
GCCGGAT	20	7.0300006E-4	45.0	17
CGCGCAA	20	7.0300006E-4	45.0	18
TCGTGCG	20	7.0300006E-4	45.0	1
CGTGTTG	20	7.0300006E-4	45.0	30
GCAATCG	25	3.8880833E-5	45.0	21
GTGCGAA	20	7.0300006E-4	45.0	9
GCGTACG	25	3.8880833E-5	45.0	1
CGTTTTT	20770	0.0	44.393356	1
GTTTTTT	23205	0.0	39.909504	2
TACGGCA	40	3.4537334E-7	39.375	41
TAGGGTC	80	0.0	39.375	5
TACGGGA	75	0.0	39.0	4
GCGTTAG	35	6.242213E-6	38.571426	1
GCTCGTA	65	9.094947E-12	38.076927	32
AGTACGG	65	9.094947E-12	38.076927	2
CTCGTAC	60	1.546141E-10	37.500004	29
>>END_MODULE