##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373804
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37721907737745	33.0	31.0	34.0	30.0	34.0
2	32.58117890659276	34.0	31.0	34.0	31.0	34.0
3	32.42748873741319	34.0	31.0	34.0	30.0	34.0
4	36.05120062920675	37.0	35.0	37.0	35.0	37.0
5	36.08153738322758	37.0	35.0	37.0	35.0	37.0
6	36.10243871119624	37.0	36.0	37.0	35.0	37.0
7	36.24778226022193	37.0	37.0	37.0	35.0	37.0
8	35.993852393232814	37.0	37.0	37.0	35.0	37.0
9	37.82466747279323	39.0	38.0	39.0	35.0	39.0
10	37.65378647633519	39.0	38.0	39.0	35.0	39.0
11	37.65300531829515	39.0	37.0	39.0	35.0	39.0
12	37.58042717573916	39.0	37.0	39.0	35.0	39.0
13	37.57142780708607	39.0	37.0	39.0	35.0	39.0
14	38.9783496163765	41.0	39.0	41.0	35.0	41.0
15	39.065435361847385	41.0	39.0	41.0	35.0	41.0
16	39.11414270580304	41.0	39.0	41.0	35.0	41.0
17	39.06495382606928	41.0	39.0	41.0	35.0	41.0
18	38.41317909920707	39.0	38.0	40.0	35.0	41.0
19	37.7231998587495	37.0	37.0	40.0	35.0	41.0
20	36.85598602476164	36.0	35.0	40.0	34.0	41.0
21	36.83934628842923	36.0	35.0	40.0	34.0	41.0
22	36.8175835464575	35.0	35.0	40.0	34.0	41.0
23	36.82473435276241	35.0	35.0	40.0	34.0	41.0
24	36.65303474548159	35.0	35.0	40.0	34.0	41.0
25	36.57508747899969	35.0	35.0	40.0	33.0	41.0
26	36.43099057259954	35.0	35.0	40.0	33.0	41.0
27	36.33733721415501	35.0	35.0	40.0	33.0	41.0
28	36.20188922536944	35.0	35.0	40.0	33.0	41.0
29	35.96440915560026	35.0	35.0	40.0	33.0	41.0
30	35.4647328546511	35.0	35.0	40.0	31.0	41.0
31	34.857369102524316	35.0	35.0	40.0	25.0	41.0
32	33.85630972381248	35.0	34.0	40.0	18.0	41.0
33	32.74458807289382	35.0	33.0	40.0	12.0	41.0
34	31.794539384276252	35.0	31.0	40.0	8.0	41.0
35	31.200457994028955	35.0	26.0	40.0	7.0	41.0
36	30.83523183272517	35.0	23.0	40.0	7.0	41.0
37	30.65735251629196	35.0	23.0	40.0	7.0	41.0
38	30.49410653711571	35.0	23.0	40.0	7.0	41.0
39	30.35022632181571	35.0	22.0	40.0	7.0	41.0
40	30.20530010379771	35.0	22.0	40.0	7.0	41.0
41	30.036920418187073	35.0	21.0	40.0	7.0	41.0
42	29.898858760205883	35.0	21.0	40.0	7.0	41.0
43	29.77219077377449	35.0	20.0	40.0	7.0	41.0
44	29.663647258991343	35.0	20.0	40.0	7.0	41.0
45	29.61036264994489	35.0	20.0	40.0	7.0	41.0
46	29.599710008453627	35.0	20.0	40.0	7.0	41.0
47	29.637095911226204	35.0	20.0	40.0	7.0	41.0
48	29.620180629420766	35.0	20.0	40.0	7.0	41.0
49	29.613530085285337	35.0	20.0	40.0	7.0	41.0
50	29.467787931643322	35.0	20.0	40.0	7.0	41.0
51	28.832770649859285	35.0	20.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	8.0
9	5.0
10	4.0
11	5.0
12	3.0
13	12.0
14	10.0
15	27.0
16	46.0
17	90.0
18	156.0
19	384.0
20	640.0
21	1056.0
22	1654.0
23	2785.0
24	5280.0
25	10809.0
26	18208.0
27	23624.0
28	21493.0
29	16578.0
30	12584.0
31	10693.0
32	10346.0
33	11340.0
34	17483.0
35	27567.0
36	28856.0
37	24958.0
38	44923.0
39	82086.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.994638901670392	12.560860771955356	15.114873035066505	46.329627291307744
2	57.98386320103583	16.270558902526457	15.030604273897548	10.714973622540155
3	19.942536730479073	16.011332141978148	53.49808990808017	10.548041219462606
4	18.51077035023702	15.488330782977174	54.34933815582498	11.651560710960824
5	16.026313255074854	18.745385282126463	52.542241388535174	12.686060074263516
6	15.976019518250206	21.94037516987512	52.26589335587634	9.81771195599833
7	49.20519844624456	3.9592941755572437	43.65442852403933	3.181078854158864
8	49.44543129554526	4.433874436870659	42.55411927106184	3.5665749965222417
9	45.73546564509743	4.715572867064023	43.88877593605205	5.660185551786498
10	25.94140244620175	14.396047126301484	48.96657071620422	10.695979711292548
11	19.31065478165028	16.061625878802797	51.6495275598977	12.97819177964923
12	17.31361890188441	13.753464382403614	54.6824004023499	14.250516313362082
13	15.776717210088709	15.04264266835026	55.69870841403516	13.481931707525868
14	13.255609891814963	17.615648842709014	55.06227862730201	14.066462638174016
15	13.193545280414334	15.344405089298135	57.51623845651732	13.945811173770212
16	13.916116467453532	15.409679939219483	55.584477426672805	15.089726166654183
17	13.662507624316486	15.587045617489379	54.74259237461343	16.0078543835807
18	14.11862901413575	15.633326556163125	55.74044151480455	14.507602914896578
19	15.279397759253513	16.2002011749473	54.12007362147008	14.400327444329115
20	15.304277107789108	16.29945104921296	54.965436431926896	13.430835411071044
21	15.361258841531924	16.67692159527453	54.72386598324256	13.23795357995099
22	13.895249917068838	14.75639640025254	55.065221345946	16.28313233673262
23	14.330772276380136	15.927063380809194	54.78058019710864	14.961584145702025
24	14.890423858492685	15.224823704401235	54.82686113578239	15.05789130132369
25	14.196209778386534	16.9527345881799	53.732169800216155	15.118885833217407
26	13.657424746658679	16.5709837240907	53.759456827642296	16.01213470160833
27	14.24489839595082	16.961027704358433	53.94832586061145	14.845748039079304
28	13.090817647751226	16.476549207606126	55.50047618538058	14.932156959262075
29	14.621031342628758	15.95542048774224	54.203004783255395	15.220543386373608
30	14.102310301655413	17.166215449807922	53.326877186975	15.404597061561672
31	15.500904217183336	17.557329509582562	50.974307391039154	15.967458882194945
32	16.137869043669944	19.246450011235837	49.261110100480465	15.354570844613754
33	15.999026227648713	19.52868348118265	46.92994189468278	17.54234839648586
34	15.682282693604135	20.73305796620689	45.49362767653637	18.09103166365261
35	16.983231854126764	21.229574857411908	43.24405303314036	18.54314025532097
36	18.37620785224342	22.661608757530686	41.771623631635826	17.190559758590062
37	17.129030186942888	23.686744925147938	41.734438368770796	17.44978651913837
38	16.866860707750586	24.645001123583484	40.77297193181453	17.7151662368514
39	17.667815218670746	23.659190377845075	40.30427710778911	18.36871729569507
40	17.61083348492793	22.881777616076874	41.31898000021402	18.18840889878118
41	16.823790007597562	23.893805309734514	39.29626221228237	19.98614247038555
42	17.72934479031792	23.93794608939444	39.6552738868498	18.677435233437844
43	18.62259365870884	23.272356636098063	39.65901916512397	18.446030540069128
44	17.31281634225423	23.24239440990466	40.26093888775936	19.183850360081752
45	17.136253223614514	22.53346673657853	40.34146237065414	19.988817669152816
46	17.966634920974627	22.482102920246973	41.3746241345732	18.1766380242052
47	15.964516163550952	22.417630629955806	43.60252966795433	18.01532353853891
48	16.459962975249063	21.597147168034585	42.975463076906614	18.967426779809742
49	16.929460358904667	20.483996960974203	43.933719275342156	18.652823404778974
50	16.465313372783598	20.398925640175065	43.968229339439915	19.167531647601415
51	15.724818354003704	20.766497950797742	42.131170345956704	21.37751334924185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	218.0
2	329.0
3	25049.5
4	49770.0
5	32110.0
6	14450.0
7	13788.5
8	13127.0
9	12718.5
10	12310.0
11	11799.5
12	11289.0
13	10430.5
14	9572.0
15	8817.5
16	8063.0
17	7218.0
18	6373.0
19	5752.5
20	5132.0
21	4670.5
22	4209.0
23	3857.5
24	3506.0
25	3389.5
26	3201.0
27	3129.0
28	3175.0
29	3221.0
30	3398.5
31	3576.0
32	3785.0
33	3994.0
34	4687.0
35	5380.0
36	5747.5
37	6115.0
38	6417.5
39	6720.0
40	7743.0
41	8766.0
42	10307.0
43	11848.0
44	14001.5
45	16155.0
46	19512.5
47	22870.0
48	24119.0
49	25368.0
50	24611.0
51	23854.0
52	20742.0
53	17630.0
54	16289.0
55	14948.0
56	13565.0
57	12182.0
58	11223.5
59	10265.0
60	9903.5
61	9542.0
62	8638.5
63	7735.0
64	6801.5
65	5868.0
66	5131.0
67	4394.0
68	3709.5
69	3025.0
70	2752.5
71	2480.0
72	1968.0
73	1456.0
74	1177.0
75	669.5
76	441.0
77	323.5
78	206.0
79	159.0
80	112.0
81	93.5
82	75.0
83	49.0
84	23.0
85	14.0
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	4.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	373804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.0204990326639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.46578975374308	34.61678430263441
2	7.69557241006462	6.6213473084596
3	2.6564384897535382	3.4284392843631974
4	1.4280528662749068	2.457421878086902
5	0.9525551207444376	2.0489698325272565
6	0.7589947578925479	1.9591399948627999
7	0.6513306682658041	1.9614399268851352
8	0.5923144250465718	2.038532971979914
9	0.507656682224561	1.965567942591038
>10	4.182040452229095	28.56060175294644
>50	0.07785943877213454	2.2631085407392524
>100	0.02699964409033698	2.265539667125819
>500	0.003767392198651672	1.2631052203982747
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	6.278986997752786E-4	8.550001376399955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31652	8.467539138157964	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	993	0.26564723758975295	No Hit
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	879	0.23514997164289306	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	842	0.22525173620399994	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	767	0.2051877454494869	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	656	0.17549303913280756	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	539	0.1441932135557672	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	483	0.12921210045906412	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	483	0.12921210045906412	No Hit
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	479	0.12814202095215677	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	385	0.10299515253983371	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.350397534536816E-4	0.0	0.0	0.13188729922633252	0.0
2	5.350397534536816E-4	0.0	0.0	0.7043798354217718	0.0
3	5.350397534536816E-4	0.0	0.0	0.9638741158468074	0.0
4	5.350397534536816E-4	0.0	0.0	1.283827888412109	0.0
5	5.350397534536816E-4	0.0	0.0	2.258670319204717	0.0
6	5.350397534536816E-4	0.0	0.0	2.7011481953109118	0.0
7	5.350397534536816E-4	0.0	0.0	3.1973975666392014	0.0
8	5.350397534536816E-4	0.0	0.0	3.9279943499802035	0.0
9	5.350397534536816E-4	0.0	0.0	4.180265593733615	0.0
10	5.350397534536816E-4	0.0	0.0	4.959550994638902	0.0
11	5.350397534536816E-4	0.0	0.0	5.839691389070208	0.0
12	5.350397534536816E-4	0.0	0.0	6.797412547752298	0.0
13	5.350397534536816E-4	0.0	0.0	7.098104889193267	0.0
14	5.350397534536816E-4	0.0	0.0	7.229189628789419	0.0
15	5.350397534536816E-4	0.0	0.0	7.472632716610844	0.0
16	5.350397534536816E-4	0.0	0.0	7.957111213363153	0.0
17	5.350397534536816E-4	0.0	0.0	8.487335609035751	0.0
18	5.350397534536816E-4	0.0	0.0	9.07775197697189	0.0
19	5.350397534536816E-4	0.0	0.0	9.486522348610501	0.0
20	5.350397534536816E-4	0.0	0.0	9.848476741821917	0.0
21	8.025596301805225E-4	0.0	0.0	10.276508544584862	0.0
22	8.025596301805225E-4	0.0	0.0	10.737712812061936	0.0
23	8.025596301805225E-4	0.0	0.0	11.156381419139443	0.0
24	8.025596301805225E-4	0.0	0.0	11.515125573830135	0.0
25	8.025596301805225E-4	0.0	0.0	11.836951985532526	0.0
26	8.025596301805225E-4	0.0	0.0	12.128816171041509	0.0
27	0.0010700795069073631	0.0	0.0	12.427368353468662	0.0
28	0.0013375993836342041	0.0	0.0	12.740634129115794	0.0
29	0.0013375993836342041	0.0	0.0	13.032765834501504	0.0
30	0.0013375993836342041	0.0	0.0	13.366363120779875	0.0
31	0.0013375993836342041	0.0	0.0	13.672138339878654	0.0
32	0.0013375993836342041	0.0	0.0	13.996372430471585	0.0
33	0.0013375993836342041	0.0	0.0	14.297064771912552	0.0
34	0.0013375993836342041	0.0	0.0	14.585183679147361	0.0
35	0.0013375993836342041	0.0	0.0	14.907277610726476	0.0
36	0.0013375993836342041	0.0	0.0	15.201549475126003	0.0
37	0.0013375993836342041	0.0	0.0	15.50491701533424	0.0
38	0.0013375993836342041	0.0	0.0	15.793035922569047	0.0
39	0.0013375993836342041	0.0	0.0	16.098276101914372	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	30	2.1615579E-6	45.000004	14
CGTATGG	35	1.2089913E-7	45.000004	2
TCCTCGC	30	2.1615579E-6	45.000004	39
ATATCGG	30	2.1615579E-6	45.000004	2
AACATAC	25	3.8857375E-5	45.000004	16
CATACGA	25	3.8857375E-5	45.000004	18
GACGAGG	35	1.2089913E-7	45.000004	2
AGGGCGC	55	1.8189894E-12	45.000004	6
TCGACAA	25	3.8857375E-5	45.000004	19
CGAGTAG	20	7.027173E-4	45.0	1
TAGAACG	20	7.027173E-4	45.0	1
GTAGGGT	95	0.0	45.0	4
CGACAAT	20	7.027173E-4	45.0	20
AATGCGG	20	7.027173E-4	45.0	2
GTATACG	20	7.027173E-4	45.0	1
CGTAAAT	20	7.027173E-4	45.0	16
GGCGAAC	20	7.027173E-4	45.0	32
CGAAGCC	20	7.027173E-4	45.0	12
CGTTTTT	13950	0.0	44.451614	1
AAGGGCG	100	0.0	40.500004	5
>>END_MODULE