##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935654.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	710422
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.467617838411535	34.0	31.0	34.0	31.0	34.0
2	32.67522683700674	34.0	31.0	34.0	31.0	34.0
3	32.5103600395258	34.0	31.0	34.0	31.0	34.0
4	36.10666617869379	37.0	35.0	37.0	35.0	37.0
5	36.12923304739999	37.0	35.0	37.0	35.0	37.0
6	36.13702278364127	37.0	37.0	37.0	35.0	37.0
7	36.265036837260105	37.0	37.0	37.0	35.0	37.0
8	36.02465576798016	37.0	37.0	37.0	35.0	37.0
9	37.825607878134406	39.0	38.0	39.0	35.0	39.0
10	37.70920804817418	39.0	38.0	39.0	35.0	39.0
11	37.700814445498594	39.0	38.0	39.0	35.0	39.0
12	37.644031294075916	39.0	38.0	39.0	35.0	39.0
13	37.652388298785795	39.0	38.0	39.0	35.0	39.0
14	39.099384591130345	41.0	39.0	41.0	35.0	41.0
15	39.18123734906858	41.0	39.0	41.0	35.0	41.0
16	39.20963314762212	41.0	39.0	41.0	35.0	41.0
17	39.154223827527865	41.0	39.0	41.0	35.0	41.0
18	38.49162891914946	39.0	39.0	41.0	35.0	41.0
19	37.79001354124731	37.0	37.0	41.0	35.0	41.0
20	36.964880029053155	36.0	35.0	41.0	34.0	41.0
21	36.91097826362359	36.0	35.0	40.0	34.0	41.0
22	36.89411223188471	36.0	35.0	40.0	34.0	41.0
23	36.90224401834403	36.0	35.0	40.0	34.0	41.0
24	36.72545613733809	36.0	35.0	40.0	34.0	41.0
25	36.638863942839606	35.0	35.0	40.0	33.0	41.0
26	36.46770088764143	35.0	35.0	40.0	33.0	41.0
27	36.38104675812404	35.0	35.0	40.0	33.0	41.0
28	36.220010078516715	35.0	35.0	40.0	33.0	41.0
29	35.94575477673833	35.0	35.0	40.0	33.0	41.0
30	35.402469236594584	35.0	35.0	40.0	30.0	41.0
31	34.73121890932432	35.0	35.0	40.0	23.0	41.0
32	33.69739112809007	35.0	34.0	40.0	18.0	41.0
33	32.53910633398178	35.0	33.0	40.0	12.0	41.0
34	31.55432545726343	35.0	29.0	40.0	8.0	41.0
35	30.993647437720117	35.0	23.0	40.0	7.0	41.0
36	30.61135634876172	35.0	23.0	40.0	7.0	41.0
37	30.416144207245836	35.0	22.0	40.0	7.0	41.0
38	30.228333300489005	35.0	21.0	40.0	7.0	41.0
39	30.073328247154507	35.0	21.0	40.0	7.0	41.0
40	29.948717804347275	35.0	21.0	40.0	7.0	41.0
41	29.753573228306557	35.0	20.0	40.0	7.0	41.0
42	29.631893437984747	35.0	19.0	40.0	7.0	41.0
43	29.503540149376004	35.0	18.0	40.0	7.0	41.0
44	29.391681282392717	35.0	18.0	40.0	7.0	41.0
45	29.333170988511053	35.0	20.0	40.0	7.0	41.0
46	29.293850697191246	35.0	20.0	40.0	7.0	41.0
47	29.293453750024632	35.0	20.0	40.0	7.0	41.0
48	29.24518103324503	35.0	20.0	40.0	7.0	41.0
49	29.202441647358892	35.0	20.0	40.0	7.0	41.0
50	29.085851789499763	35.0	20.0	39.0	7.0	41.0
51	28.47176326183592	34.0	20.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	9.0
9	5.0
10	13.0
11	9.0
12	17.0
13	20.0
14	30.0
15	52.0
16	96.0
17	196.0
18	427.0
19	749.0
20	1311.0
21	2167.0
22	3209.0
23	5428.0
24	10219.0
25	20969.0
26	36932.0
27	46796.0
28	42978.0
29	32537.0
30	24502.0
31	20172.0
32	18817.0
33	20429.0
34	32024.0
35	46246.0
36	50157.0
37	49516.0
38	85164.0
39	159057.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.464160738265424	12.829979927423418	14.508700462541983	47.197158871769176
2	59.41172992953484	15.424775696698582	14.709566989761017	10.453927384005562
3	20.833532745326018	15.281058300559385	53.12546627215936	10.759942681955232
4	18.71718499708624	16.15955023915363	53.143202209391035	11.980062554369093
5	16.43037518545315	18.822615290630075	52.315384377173004	12.431625146743766
6	16.57437410440555	21.893747659841615	51.719119058812936	9.812759176939903
7	49.70003744253415	4.099816728648606	42.976146572037464	3.2239992567797735
8	49.954534065668014	4.103898809440023	42.52894758326741	3.4126195416245557
9	45.94086331785896	5.323455636227481	44.08070132963225	4.654979716281309
10	25.94992835244404	16.070589030182063	48.297490787165934	9.681991830207961
11	20.05301074572578	16.343806920393796	51.33652955567254	12.266652778207881
12	18.977593599297318	13.663146693092273	53.89106756265994	13.468192144950466
13	16.664883688849727	13.854019160442665	54.939036234801286	14.54206091590632
14	12.952160828352726	17.242709262945123	54.64484489500606	15.160285013696084
15	13.247759782213953	16.090577149919344	57.22035635157695	13.441306716289755
16	14.526858683993456	15.139311564112598	56.690671178538956	13.643158573354992
17	14.365546112029188	15.208988460379889	55.08570962047909	15.33975580711183
18	14.854410477153015	16.23443530746514	54.15879575801426	14.752358457367592
19	16.48682050949999	16.863638794969752	53.02088054705513	13.628660148475133
20	18.333750925506248	15.658861915875352	53.12251028262075	12.884876875997648
21	17.005526292823138	15.896889454436941	53.4946834416727	13.602900811067226
22	15.324272052385766	15.902379149294363	53.26890214548536	15.504446652834513
23	15.834954435532683	16.500052081720444	53.29578757414607	14.369205908600804
24	15.574545833321604	15.200824298797052	53.980732578664515	15.243897289216832
25	15.771471041155822	16.03877695229033	52.70444890501702	15.485303101536832
26	14.642423798812537	17.340116156312728	52.86998431917931	15.14747572569543
27	15.521901067252985	16.91262376446675	53.05339643197986	14.512078736300396
28	14.297980636860908	17.9604516752015	53.14010545844583	14.601462229491766
29	15.80736519983897	17.47383949258328	52.26865159018161	14.450143717396138
30	15.92095965496564	17.192035156568913	51.29838321448379	15.588621973981661
31	15.873241538128042	19.416206142264738	48.83914067976499	15.871411639842234
32	17.66654185821948	20.31173021105765	47.43546793314396	14.586259997578905
33	17.735092663233964	21.32844985093367	44.569847217569276	16.366610268263088
34	16.664039120410123	23.94168536447351	43.045682706898155	16.348592808218214
35	18.23535870229244	23.385677808401205	40.36727466210224	18.01168882720411
36	17.273958295210452	26.57659813462984	38.44968201998249	17.699761550177218
37	18.82331909766308	26.555343162233154	38.22587138348756	16.395466356616208
38	18.697056115942356	25.43811987804432	37.78500665801453	18.079817347998794
39	18.764058545484232	24.51542885777749	38.111010075701486	18.60950252103679
40	18.289833366646864	24.53513545470157	40.06562296775719	17.10940821089437
41	16.76271286643713	26.16247807641092	38.90842344409379	18.166385613058154
42	18.22789834774261	26.275650247317788	37.66409260974463	17.83235879519497
43	18.681572361216293	25.520324539499057	37.54374160710113	18.25436149218352
44	18.298842096669304	25.465990636551233	37.47082719848203	18.764340068297432
45	18.07826897252619	24.042189008786327	38.14451129047242	19.73503072821506
46	17.411904473679023	25.04539555362869	38.393236695935656	19.14946327675663
47	17.31534214875102	24.4689775935993	40.04070819878889	18.17497205886079
48	17.183307949359676	23.154406817356442	40.197657167148535	19.46462806613534
49	17.158815464611173	23.040953123636374	40.54012966940776	19.26010174234469
50	15.983035435276497	22.426388822418225	41.405671558594754	20.18490418371053
51	15.919270518086433	22.456230240617547	40.724386350647926	20.900112890648092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	472.0
2	737.0
3	44997.0
4	89257.0
5	59623.0
6	29989.0
7	28590.0
8	27191.0
9	25897.5
10	24604.0
11	22964.5
12	21325.0
13	19824.0
14	18323.0
15	16827.0
16	15331.0
17	14129.5
18	12928.0
19	11771.5
20	10615.0
21	9557.5
22	8500.0
23	7753.0
24	7006.0
25	6686.0
26	6262.5
27	6159.0
28	6347.0
29	6535.0
30	7239.5
31	7944.0
32	8368.5
33	8793.0
34	9984.5
35	11176.0
36	11547.5
37	11919.0
38	12526.5
39	13134.0
40	15378.5
41	17623.0
42	18417.5
43	19212.0
44	22236.5
45	25261.0
46	28753.5
47	32246.0
48	35055.0
49	37864.0
50	37624.0
51	37384.0
52	33629.5
53	29875.0
54	27600.0
55	25325.0
56	24937.5
57	24550.0
58	25134.5
59	25719.0
60	25344.5
61	24970.0
62	23510.0
63	22050.0
64	18756.0
65	15462.0
66	13050.5
67	10639.0
68	9293.0
69	7947.0
70	6774.0
71	5601.0
72	4857.5
73	4114.0
74	3441.0
75	2313.5
76	1859.0
77	1403.5
78	948.0
79	772.0
80	596.0
81	426.0
82	256.0
83	152.0
84	48.0
85	32.5
86	17.0
87	25.5
88	34.0
89	23.0
90	12.0
91	7.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	710422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.85501093254731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73290989566921	32.166338945388276
2	7.423049619596626	6.065375467229226
3	2.80784058782711	3.4414307373757995
4	1.7164438983941206	2.8050133693598367
5	1.2773123519622478	2.609230505184767
6	1.0304656114810458	2.525981029360331
7	0.8961952013245289	2.562984532346704
8	0.7884428558387464	2.5769473195984642
9	0.7159653859212256	2.632569630222341
>10	4.467613958734655	27.82806055598921
>50	0.08368674676033712	2.4306273746600224
>100	0.055212415907142744	3.740921695952463
>500	0.0038197268866576456	1.016485416644517
>1k	6.944957975741175E-4	0.29593699638806176
>5k	0.0	0.0
>10k+	3.4724789878705874E-4	7.3020964243000615
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51471	7.2451303591386536	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1074	0.15117775068902709	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	1012	0.1424505434797909	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	897	0.12626298172072373	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	832	0.11711349029168579	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	715	0.10064440571941748	Illumina PCR Primer Index 7 (96% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8152281320116773E-4	0.0	0.0	0.07446278409170887	0.0
2	2.8152281320116773E-4	0.0	0.0	0.33121158973117387	0.0
3	2.8152281320116773E-4	0.0	0.0	0.5386939030604345	0.0
4	2.8152281320116773E-4	0.0	0.0	0.771935553797602	0.0
5	2.8152281320116773E-4	0.0	0.0	1.3914265042467717	0.0
6	2.8152281320116773E-4	0.0	0.0	1.8932409187778532	0.0
7	2.8152281320116773E-4	0.0	0.0	2.3115838191947886	0.0
8	2.8152281320116773E-4	0.0	0.0	2.9702064406789206	0.0
9	2.8152281320116773E-4	0.0	0.0	3.2824152405190157	0.0
10	2.8152281320116773E-4	0.0	0.0	3.8446162984817476	0.0
11	2.8152281320116773E-4	0.0	0.0	4.473538263173157	0.0
12	2.8152281320116773E-4	0.0	0.0	5.056431247906174	0.0
13	2.8152281320116773E-4	0.0	0.0	5.298540867259178	0.0
14	2.8152281320116773E-4	0.0	0.0	5.412698368012252	0.0
15	4.2228421980175165E-4	0.0	0.0	5.574996269822725	0.0
16	4.2228421980175165E-4	0.0	0.0	5.898325220784266	0.0
17	4.2228421980175165E-4	0.0	0.0	6.240234677417084	0.0
18	4.2228421980175165E-4	0.0	0.0	6.6319736719865094	0.0
19	4.2228421980175165E-4	0.0	0.0	6.873097961493309	0.0
20	4.2228421980175165E-4	0.0	0.0	7.115911387879317	0.0
21	4.2228421980175165E-4	0.0	0.0	7.402923895937907	0.0
22	4.2228421980175165E-4	0.0	0.0	7.712035944832789	0.0
23	4.2228421980175165E-4	0.0	0.0	8.011435456672231	0.0
24	4.2228421980175165E-4	0.0	0.0	8.286483245169773	0.0
25	4.2228421980175165E-4	0.0	0.0	8.513812916829716	0.0
26	4.2228421980175165E-4	0.0	0.0	8.725940356576794	0.0
27	4.2228421980175165E-4	0.0	0.0	8.931170487400447	0.0
28	4.2228421980175165E-4	0.0	0.0	9.149069144818151	0.0
29	4.2228421980175165E-4	0.0	0.0	9.381325465709114	0.0
30	4.2228421980175165E-4	0.0	0.0	9.65032051372283	0.0
31	4.2228421980175165E-4	0.0	0.0	9.897779066526656	0.0
32	4.2228421980175165E-4	0.0	0.0	10.15917299858394	0.0
33	4.2228421980175165E-4	0.0	0.0	10.398185866991732	0.0
34	4.2228421980175165E-4	0.0	0.0	10.623404117552665	0.0
35	4.2228421980175165E-4	0.0	0.0	10.872129523015898	0.0
36	4.2228421980175165E-4	0.0	0.0	11.131412033974174	0.0
37	4.2228421980175165E-4	0.0	0.0	11.391116829152251	0.0
38	4.2228421980175165E-4	0.0	0.0	11.62281010441681	0.0
39	4.2228421980175165E-4	0.0	0.0	11.87322464675925	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	20	7.031465E-4	45.0	1
GACCGAC	20	7.031465E-4	45.0	9
TAACGAT	25	3.889296E-5	45.0	17
ATCGTTG	50	2.1827873E-11	45.0	23
ATAACGA	25	3.889296E-5	45.0	16
CGTTTTT	27465	0.0	44.58219	1
TTACACG	70	0.0	41.785717	34
GTTTTTT	30045	0.0	40.963554	2
AATCGTT	55	6.002665E-11	40.909092	22
ACAACGA	45	1.9264917E-8	40.0	13
CTACGAA	130	0.0	39.80769	11
TAGCCGT	85	0.0	39.705883	44
CGATGCG	40	3.4557343E-7	39.375	10
CGAGATA	95	0.0	37.894737	19
GGCCGAT	60	1.546141E-10	37.499996	8
ACGATTC	30	1.1394832E-4	37.499996	19
CACTCGT	30	1.1394832E-4	37.499996	22
GTGCTAA	30	1.1394832E-4	37.499996	25
TCGTAAG	30	1.1394832E-4	37.499996	1
CGGTCTA	30	1.1394832E-4	37.499996	31
>>END_MODULE