FastQCFastQC Report
Sat 14 Jan 2017
SRR2935652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225592
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89320.7287906579024667No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC79880.6517666564403163No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC50350.4108218722054321No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG49060.4002963465818968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40890.3336346842995059No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC40040.32669926043903685TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC33500.27333729332436896TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG32410.26444363213859096TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT28400.23172475016155458No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT27430.22381020763843104No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG25960.211816004020914No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT24540.20022976651283628TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT23080.18831715611720704No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC20440.166776545538809No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT16870.13764776532483894No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC15230.12426647693522803No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC12580.10264427313494214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG207.033469E-445.0000041
TAACGCC406.8157533E-945.00000412
ATAACGC406.8157533E-945.00000411
TCGCTAA253.890958E-545.032
ACTACGC351.2120108E-745.023
CGTTTTT33600.041.4508931
TCGTTTA501.0822987E-940.538
GCCGATA451.9284926E-840.09
CGTCATA403.4584627E-739.37500438
TATTGCG750.039.0000041
TAGCCGT1100.038.86363644
TCACGAC700.038.57142625
ATTAGCG356.2486306E-638.5714261
ATTAACG356.2486306E-638.5714261
TTAACGG900.037.52
GTAAGCG900.037.51
TACCCGC601.5643309E-1037.49999628
GTTAACG301.1399662E-437.4999961
TTACCCG601.5643309E-1037.49999627
TAGGGCG3550.037.3943635
Produced by FastQC (version 0.11.5)