##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935652.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225592
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05917058858086	33.0	31.0	34.0	30.0	34.0
2	32.08079279238115	33.0	31.0	34.0	30.0	34.0
3	32.07343063597021	33.0	31.0	34.0	30.0	34.0
4	35.7588512327104	37.0	35.0	37.0	33.0	37.0
5	35.82390469258938	37.0	35.0	37.0	35.0	37.0
6	35.757478018786024	37.0	35.0	37.0	35.0	37.0
7	36.02918834326595	37.0	35.0	37.0	35.0	37.0
8	35.84537431706473	37.0	35.0	37.0	35.0	37.0
9	37.66118822577171	39.0	38.0	39.0	35.0	39.0
10	37.319373820977944	39.0	37.0	39.0	34.0	39.0
11	37.307637451941595	39.0	37.0	39.0	34.0	39.0
12	37.15793836774392	39.0	37.0	39.0	34.0	39.0
13	37.05341663457333	39.0	37.0	39.0	33.0	39.0
14	37.96586873935208	40.0	37.0	41.0	33.0	41.0
15	38.13236868386869	40.0	37.0	41.0	33.0	41.0
16	37.995988061279775	40.0	37.0	41.0	33.0	41.0
17	38.05535692138982	40.0	37.0	41.0	33.0	41.0
18	37.966288944444806	40.0	37.0	41.0	33.0	41.0
19	37.810028133342904	40.0	36.0	41.0	33.0	41.0
20	37.904436386660485	40.0	36.0	41.0	33.0	41.0
21	37.83450936363814	40.0	36.0	41.0	33.0	41.0
22	37.97108254623072	40.0	36.0	41.0	34.0	41.0
23	38.00625085672883	40.0	36.0	41.0	34.0	41.0
24	37.91852835201274	40.0	36.0	41.0	34.0	41.0
25	37.66636694756493	39.0	35.0	41.0	33.0	41.0
26	37.71136805723275	40.0	35.0	41.0	33.0	41.0
27	37.7239644188278	40.0	35.0	41.0	33.0	41.0
28	37.593669018727276	40.0	35.0	41.0	33.0	41.0
29	37.554970985450296	40.0	35.0	41.0	33.0	41.0
30	37.35286457483404	39.0	35.0	41.0	33.0	41.0
31	37.27582343879529	39.0	35.0	41.0	33.0	41.0
32	37.215600297652074	39.0	35.0	41.0	33.0	41.0
33	37.123860142690226	39.0	35.0	41.0	32.0	41.0
34	37.016356177259645	39.0	35.0	41.0	32.0	41.0
35	36.89830873569671	39.0	35.0	41.0	31.0	41.0
36	36.782683796891625	39.0	35.0	41.0	31.0	41.0
37	36.76780119321928	39.0	35.0	41.0	31.0	41.0
38	36.60209923041273	39.0	35.0	41.0	31.0	41.0
39	36.52181068414285	39.0	35.0	41.0	31.0	41.0
40	36.38863667517412	39.0	35.0	41.0	30.0	41.0
41	36.23329949934399	39.0	35.0	41.0	30.0	41.0
42	36.28112047076025	39.0	35.0	41.0	30.0	41.0
43	36.23205846643908	39.0	35.0	41.0	30.0	41.0
44	36.108505114263146	38.0	35.0	41.0	30.0	41.0
45	36.08913733118362	38.0	35.0	40.0	30.0	41.0
46	35.977304845331886	38.0	35.0	40.0	30.0	41.0
47	35.87824088277338	38.0	35.0	40.0	30.0	41.0
48	35.7678713633901	38.0	35.0	40.0	30.0	41.0
49	35.76484833451916	38.0	35.0	40.0	29.0	41.0
50	35.61149305804868	38.0	35.0	40.0	29.0	41.0
51	34.40294894222547	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	33.0
10	33.0
11	31.0
12	24.0
13	41.0
14	47.0
15	72.0
16	111.0
17	196.0
18	387.0
19	641.0
20	1056.0
21	1800.0
22	2936.0
23	4197.0
24	6019.0
25	8539.0
26	11102.0
27	12937.0
28	14472.0
29	16328.0
30	19569.0
31	24408.0
32	32454.0
33	46503.0
34	87883.0
35	97597.0
36	90575.0
37	133349.0
38	235248.0
39	376778.0
40	213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.368259583939846	18.701574422809546	21.82520773634293	17.104958256907683
2	32.2768914940698	25.53957597634449	24.513704397548285	17.669828132037416
3	31.492209479174143	24.58877016168513	27.406510486360876	16.512509872779848
4	28.02963792191855	23.80833099432764	28.725301731734543	19.43672935201927
5	25.542513332332454	27.957264734104008	26.77008335563548	19.730138577928056
6	24.41701642961116	33.16470734143173	27.057291496680786	15.36098473227632
7	76.19183219211614	5.841177161730821	12.49086155914856	5.476129087004485
8	76.62207325113089	6.557892022793882	11.118218787328898	5.7018159387463365
9	70.79125842857982	6.962839183023388	13.69362724299767	8.552275145399122
10	41.301101834868376	22.370740017885236	20.616648933739775	15.711509213506616
11	31.14910998113565	24.422156802590095	25.21605885155908	19.212674364715173
12	28.21787348481387	20.138349467033077	30.9328063499109	20.710970698242157
13	26.19444317521655	21.45322423775612	33.05847296653373	19.293859620493606
14	20.473044863217122	25.53851526445995	33.158751036233916	20.82968883608901
15	19.07062056540839	24.527085685937898	35.28825253428547	21.11404121436824
16	23.76720801049615	24.235797883798195	30.247015319943344	21.74997878576231
17	23.428269766773933	24.627526942081868	28.476605591420313	23.46759769972389
18	24.360553920064753	23.777488756454023	29.89559331327228	21.966364010208945
19	23.952506217403506	26.749929829829178	26.706359049341057	22.591204903426263
20	23.64277834711715	28.066273278546205	27.40822394402052	20.882724430316124
21	23.857858080013578	27.290729704501988	29.411990287142864	19.43942192834157
22	21.713343429134653	23.340393866800696	30.595581563848327	24.35068114021632
23	21.339075320334988	25.634468893400086	29.95409565336588	23.07236013289904
24	22.769975652582588	25.290145497033272	28.757286274714588	23.182592575669553
25	23.844068825514526	26.76902264375094	25.890997983015556	23.495910547718978
26	20.7369173428025	25.1762413592778	28.1470505682152	25.939790729704505
27	22.144808386477717	24.033038727406836	28.739090986233588	25.083061899881855
28	20.17351614566675	24.654371112082977	29.775324904209562	25.39678783804072
29	21.819577804032665	24.90600460838517	29.667132291986242	23.607285295595926
30	22.468488697706903	23.860958622445317	30.471233493691212	23.19931918615657
31	22.79559592425538	24.091785847166104	29.53299303520258	23.579625193375936
32	23.360873765494553	24.101413847348873	29.073460009530088	23.464252377626487
33	22.70364036318775	23.949568861415543	27.078668920815407	26.268121854581295
34	20.052431804385147	23.984490760383554	30.68443658248422	25.27864085274708
35	19.418044504206946	24.734577249198754	28.53339447385427	27.31398377274003
36	21.435436915384564	25.23694671636238	26.967212579716577	26.36040378853648
37	19.68436478044896	25.092934679730284	28.912476582745317	26.31022395707544
38	20.881337345544033	25.85093571106861	28.200330942107975	25.06739600127938
39	20.96284897421001	23.931128793268886	27.49658940332509	27.609432829196013
40	22.8762100274806	23.255128949927872	29.077294890958814	24.791366131632714
41	20.187713366275236	22.915864333318105	27.713219407437386	29.183202892969273
42	20.611345374317064	22.937568130340278	29.70621544527053	26.744871050072128
43	21.268170810514427	21.975992010391714	30.270106201737608	26.485730977356248
44	22.47330269779829	22.73121887218585	28.80044908909327	25.995029340922592
45	21.34756101541133	22.297061338520486	28.20098368788308	28.154393958185103
46	22.50308422378736	22.956008198486934	28.044406295080254	26.496501282645447
47	19.492131149681132	23.46221254707929	31.2462875084041	25.799368794835477
48	19.957212514442	24.25456432483241	29.888657889411814	25.89956527131378
49	21.806930854640044	22.227625506693908	30.7269466510878	25.238496987578245
50	20.69962924039974	22.26703503286575	30.339542033564186	26.69379369317032
51	20.166009569253063	22.172223709032043	28.546449389356326	29.115317332358565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4834.0
1	4178.0
2	3522.0
3	8565.5
4	13609.0
5	8616.0
6	3623.0
7	3512.5
8	3402.0
9	3460.0
10	3518.0
11	3427.5
12	3337.0
13	3279.0
14	3221.0
15	3151.0
16	3081.0
17	3092.5
18	3104.0
19	3074.5
20	3045.0
21	3432.0
22	3819.0
23	3867.0
24	3915.0
25	4597.5
26	6180.5
27	7081.0
28	8147.0
29	9213.0
30	11206.5
31	13200.0
32	15042.5
33	16885.0
34	20515.0
35	24145.0
36	26673.5
37	29202.0
38	32436.5
39	35671.0
40	40412.5
41	45154.0
42	49990.5
43	54827.0
44	62315.5
45	69804.0
46	95302.5
47	120801.0
48	123102.5
49	125404.0
50	125308.5
51	125213.0
52	108865.5
53	92518.0
54	82223.5
55	71929.0
56	68610.5
57	65292.0
58	62006.5
59	58721.0
60	55038.0
61	51355.0
62	45991.5
63	40628.0
64	36792.0
65	32956.0
66	28593.0
67	24230.0
68	20438.0
69	16646.0
70	14564.5
71	12483.0
72	10592.5
73	8702.0
74	7054.5
75	4393.5
76	3380.0
77	2687.0
78	1994.0
79	1326.5
80	659.0
81	565.5
82	472.0
83	312.5
84	153.0
85	114.0
86	75.0
87	57.5
88	40.0
89	31.0
90	22.0
91	15.5
92	9.0
93	7.5
94	6.0
95	4.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1225592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.05170840881281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87070257875743	21.170184028633845
2	10.424065711509042	6.05673834970129
3	3.924984511257241	3.4208251659045605
4	2.0322773802012652	2.361645194217327
5	1.2459935555354609	1.809912072733806
6	0.9034678233985115	1.5748370257271005
7	0.7138601105614022	1.451719904369891
8	0.5447778216398362	1.2661381137495005
9	0.447817350433041	1.1708873176669066
>10	6.485613739956252	44.48370574906545
>50	0.34011195943978506	6.034180602000789
>100	0.05531992184610525	2.834057088447736
>500	0.005927134481001977	1.2693112206173567
>1k	0.004233667486429984	3.2956173500680106
>5k	8.467334972859967E-4	1.800240817096516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8932	0.7287906579024667	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	7988	0.6517666564403163	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC	5035	0.4108218722054321	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	4906	0.4002963465818968	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4089	0.3336346842995059	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	4004	0.32669926043903685	TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	3350	0.27333729332436896	TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	3241	0.26444363213859096	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	2840	0.23172475016155458	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	2743	0.22381020763843104	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2596	0.211816004020914	No Hit
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	2454	0.20022976651283628	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	2308	0.18831715611720704	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	2044	0.166776545538809	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	1687	0.13764776532483894	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	1523	0.12426647693522803	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1258	0.10264427313494214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.527457751029707E-4	0.0	0.0	0.2114080379114746	0.0
2	6.527457751029707E-4	0.0	0.0	0.9918472052689639	0.0
3	6.527457751029707E-4	0.0	0.0	1.559409656720997	0.0
4	6.527457751029707E-4	0.0	0.0	2.421686825632021	0.0
5	6.527457751029707E-4	0.0	0.0	4.438752863922089	0.0
6	6.527457751029707E-4	0.0	0.0	5.98225184237495	0.0
7	6.527457751029707E-4	0.0	0.0	7.200438645160869	0.0
8	6.527457751029707E-4	0.0	0.0	8.744100810057507	0.0
9	6.527457751029707E-4	0.0	0.0	9.538410825135934	0.0
10	6.527457751029707E-4	0.0	0.0	11.210092755174642	0.0
11	6.527457751029707E-4	0.0	0.0	12.680647393259747	0.0
12	6.527457751029707E-4	0.0	0.0	14.233366405785938	0.0
13	6.527457751029707E-4	0.0	0.0	14.805008518332365	0.0
14	6.527457751029707E-4	0.0	0.0	15.132034151658953	0.0
15	6.527457751029707E-4	0.0	0.0	15.52808765070268	0.0
16	6.527457751029707E-4	0.0	0.0	16.082839966318318	0.0
17	6.527457751029707E-4	0.0	0.0	16.704253944216347	0.0
18	6.527457751029707E-4	0.0	0.0	17.44805775494618	0.0
19	7.34338996990842E-4	0.0	0.0	17.935822035391876	0.0
20	8.159322188787133E-4	0.0	0.0	18.41248963766082	0.0
21	8.159322188787133E-4	0.0	0.0	18.932075274642784	0.0
22	8.159322188787133E-4	0.0	0.0	19.521504709560766	0.0
23	8.975254407665846E-4	0.0	0.0	20.045088414415236	0.0
24	8.975254407665846E-4	0.0	0.0	20.483244015953108	0.0
25	8.975254407665846E-4	0.0	0.0	20.87937910821872	0.0
26	9.79118662654456E-4	0.0	0.0	21.266294166411008	0.0
27	9.79118662654456E-4	0.0	0.0	21.772416921781474	0.0
28	9.79118662654456E-4	0.0	0.0	22.19653848915463	0.0
29	9.79118662654456E-4	0.0	0.0	22.658274531818094	0.0
30	9.79118662654456E-4	0.0	0.0	23.247214407404748	0.0
31	9.79118662654456E-4	0.0	0.0	23.680882381738783	0.0
32	9.79118662654456E-4	0.0	0.0	24.10614625421837	0.0
33	9.79118662654456E-4	0.0	0.0	24.55678561870508	0.0
34	9.79118662654456E-4	0.0	0.0	25.014360407052266	0.0
35	9.79118662654456E-4	0.0	0.0	25.52619468795488	0.0
36	9.79118662654456E-4	0.0	0.0	25.93766930593542	0.0
37	9.79118662654456E-4	0.0	0.0	26.385371314434167	0.0
38	0.0010607118845423274	0.0	0.0	26.88268200184074	0.0
39	0.0010607118845423274	0.0	0.0	27.659857440322718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	20	7.033469E-4	45.000004	1
TAACGCC	40	6.8157533E-9	45.000004	12
ATAACGC	40	6.8157533E-9	45.000004	11
TCGCTAA	25	3.890958E-5	45.0	32
ACTACGC	35	1.2120108E-7	45.0	23
CGTTTTT	3360	0.0	41.450893	1
TCGTTTA	50	1.0822987E-9	40.5	38
GCCGATA	45	1.9284926E-8	40.0	9
CGTCATA	40	3.4584627E-7	39.375004	38
TATTGCG	75	0.0	39.000004	1
TAGCCGT	110	0.0	38.863636	44
TCACGAC	70	0.0	38.571426	25
ATTAGCG	35	6.2486306E-6	38.571426	1
ATTAACG	35	6.2486306E-6	38.571426	1
TTAACGG	90	0.0	37.5	2
GTAAGCG	90	0.0	37.5	1
TACCCGC	60	1.5643309E-10	37.499996	28
GTTAACG	30	1.1399662E-4	37.499996	1
TTACCCG	60	1.5643309E-10	37.499996	27
TAGGGCG	355	0.0	37.394363	5
>>END_MODULE