##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935650.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336764
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.013225879250754	33.0	31.0	34.0	30.0	34.0
2	32.021406682424484	33.0	31.0	34.0	30.0	34.0
3	32.04430996187241	33.0	31.0	34.0	30.0	34.0
4	35.71400446603556	37.0	35.0	37.0	33.0	37.0
5	35.78759012245965	37.0	35.0	37.0	35.0	37.0
6	35.73306826145313	37.0	35.0	37.0	35.0	37.0
7	36.00900333764892	37.0	35.0	37.0	35.0	37.0
8	35.78630435557245	37.0	35.0	37.0	35.0	37.0
9	37.60725908945137	39.0	37.0	39.0	35.0	39.0
10	37.25794918696773	39.0	37.0	39.0	34.0	39.0
11	37.26892126236771	39.0	37.0	39.0	34.0	39.0
12	37.09041643406065	39.0	37.0	39.0	34.0	39.0
13	36.915768906415174	39.0	35.0	39.0	33.0	39.0
14	37.99163806107541	40.0	37.0	41.0	33.0	41.0
15	38.096058367283916	40.0	37.0	41.0	33.0	41.0
16	37.972440640923615	40.0	36.0	41.0	33.0	41.0
17	37.982988086612586	40.0	36.0	41.0	33.0	41.0
18	37.90715159577627	40.0	36.0	41.0	33.0	41.0
19	37.77280528797615	40.0	36.0	41.0	33.0	41.0
20	37.87258733118742	40.0	36.0	41.0	33.0	41.0
21	37.840980627382976	40.0	36.0	41.0	33.0	41.0
22	37.97145775676735	40.0	36.0	41.0	34.0	41.0
23	37.99553099499946	40.0	36.0	41.0	34.0	41.0
24	37.92010131724294	40.0	36.0	41.0	34.0	41.0
25	37.700175790761485	40.0	35.0	41.0	33.0	41.0
26	37.77525507477046	40.0	35.0	41.0	33.0	41.0
27	37.77651708614935	40.0	35.0	41.0	33.0	41.0
28	37.65966967965697	40.0	35.0	41.0	33.0	41.0
29	37.6438010001069	40.0	36.0	41.0	33.0	41.0
30	37.52721193476737	40.0	35.0	41.0	33.0	41.0
31	37.49283474480645	40.0	35.0	41.0	33.0	41.0
32	37.436569823377795	40.0	35.0	41.0	33.0	41.0
33	37.35849140644487	40.0	35.0	41.0	33.0	41.0
34	37.29752586380967	40.0	35.0	41.0	33.0	41.0
35	37.227355061704934	40.0	35.0	41.0	32.0	41.0
36	37.10582485063724	40.0	35.0	41.0	32.0	41.0
37	37.113192027651415	40.0	35.0	41.0	32.0	41.0
38	36.94766364575786	39.0	35.0	41.0	32.0	41.0
39	36.914097112517965	39.0	35.0	41.0	32.0	41.0
40	36.82345203168985	39.0	35.0	41.0	31.0	41.0
41	36.660542694587306	39.0	35.0	41.0	31.0	41.0
42	36.74086897649392	39.0	35.0	41.0	31.0	41.0
43	36.60533489327838	39.0	35.0	41.0	31.0	41.0
44	36.55286788374054	39.0	35.0	41.0	31.0	41.0
45	36.56103383972159	39.0	35.0	41.0	31.0	41.0
46	36.43597890510862	39.0	35.0	41.0	31.0	41.0
47	36.337580620256325	39.0	35.0	41.0	31.0	41.0
48	36.25997137461249	38.0	35.0	40.0	31.0	41.0
49	36.2228474540034	38.0	35.0	40.0	31.0	41.0
50	36.10187549738095	38.0	35.0	40.0	30.0	41.0
51	34.9651239443646	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	7.0
11	4.0
12	8.0
13	11.0
14	7.0
15	20.0
16	20.0
17	37.0
18	73.0
19	165.0
20	343.0
21	580.0
22	805.0
23	1122.0
24	1483.0
25	1990.0
26	2518.0
27	3038.0
28	3276.0
29	3832.0
30	4938.0
31	6353.0
32	8554.0
33	11931.0
34	22536.0
35	30961.0
36	22662.0
37	34479.0
38	64204.0
39	110741.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86702854224323	18.50969818626694	22.970685702747325	13.652587568742502
2	29.208882184556543	28.566889572519628	24.655842073380764	17.568386169543064
3	29.93490990723474	27.320319274031668	26.486203988549846	16.25856683018375
4	28.0677269541875	24.692366167405066	29.757634426482642	17.48227245192479
5	26.915584801225783	29.416742882255825	24.302775831145848	19.364896485372547
6	23.060065802758015	36.63871435189034	25.08641066147213	15.214809183879513
7	75.96655224430164	6.818424772244064	12.642384577924007	4.572638405530283
8	75.75334655723296	8.94691831668468	9.86299010583079	5.436745020251571
9	70.22543977384757	8.521694717962728	11.03176111460845	10.221104393581262
10	41.32270670261667	24.208347685619604	19.269874452138588	15.199071159625138
11	33.94246415887684	24.649903196303644	23.62960411445404	17.77802853036548
12	30.03022888432255	20.398854984499533	28.394662137283085	21.176253993894832
13	24.29624306636101	25.49649012364742	30.35894573054127	19.848321079450297
14	19.714102457507334	29.60411445403903	27.949246356498914	22.732536731954724
15	17.776840754950054	25.730184936632185	37.05235714031191	19.440617168105852
16	21.227922224465797	24.310496371346105	32.03846016795144	22.42312123623665
17	21.005511277927567	25.295459134586835	28.873335629699138	24.825693957786463
18	21.427171550403248	26.061574277535605	29.806630162368897	22.70462400969225
19	23.930111294556426	27.49284365312207	26.38257058355406	22.194474468767446
20	26.298832416766636	26.10552196790631	28.67052297751541	18.925122637811644
21	23.72818947393427	29.73239419890487	26.723759071634735	19.815657255526126
22	20.70500409782518	26.044054590158094	28.10454799206566	25.146393319951066
23	20.851991305483956	28.63310805192954	28.110189925288925	22.404710717297576
24	23.20556829114751	24.729187205283225	28.197194474468766	23.868050029100498
25	20.996602962311886	29.093371025406515	26.417609958309086	23.492416053972516
26	20.267011913387417	26.85471131118528	28.61885474694445	24.259422028482856
27	22.007696784691948	26.9601263793042	27.68288771958998	23.349289116413868
28	19.118136142818116	28.191255597391645	30.47980187906071	22.21080638072953
29	21.822997707593448	26.35762729983015	28.213526386430853	23.60584860614555
30	21.193773681272347	27.159969592949366	27.970032426268844	23.67622429950945
31	23.4674727702486	28.13186682662042	24.50261904479101	23.898041358339967
32	25.497677899062843	28.01991899371667	24.254077039113444	22.228326068107044
33	22.90624888646055	28.636077490468104	24.962882018267987	23.494791604803364
34	22.817759618011426	26.955078333788645	28.51106412799468	21.716097920205247
35	22.006509009276527	25.522918126640615	26.651007827439987	25.81956503664287
36	22.75955862265563	28.287465406041022	27.3972277321804	21.555748239122945
37	20.86179045266121	27.759202290031	28.532147141618463	22.846860115689324
38	21.41677851551829	29.63915382879405	25.114323383734604	23.82974427195306
39	22.41183736979012	25.43531968975306	29.447031155349144	22.70581178510767
40	23.947630981933937	25.321293249872312	29.303310330082788	21.427765438110963
41	19.818329750210832	25.414533619983132	30.083084890308943	24.684051739497097
42	22.86408285921298	25.239336746208025	28.28538679906403	23.61119359551496
43	22.70432706583839	25.224786497369077	27.890748417289256	24.18013801950327
44	21.23683054008148	26.058307895143184	28.046940884417577	24.65792068035776
45	21.43251653977266	25.5440011402644	26.09483198916749	26.92865033079545
46	23.217446045301752	27.481559786675536	26.6346759154779	22.66631825254481
47	20.518226413749687	25.667826727322396	31.083488733950183	22.73045812497773
48	21.918316684681262	24.3199985746695	28.373579123659297	25.388105616989943
49	20.968690240049412	23.21002244895535	31.497725410079465	24.323561900915774
50	20.859117957976505	24.05660937629913	29.298856172274945	25.78541649344942
51	19.859901889750688	24.070565737430368	26.768894537420863	29.300637835398085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	690.0
1	796.5
2	903.0
3	1261.0
4	1619.0
5	1170.5
6	722.0
7	735.0
8	748.0
9	976.0
10	1204.0
11	978.0
12	752.0
13	761.0
14	770.0
15	797.0
16	824.0
17	839.0
18	854.0
19	795.0
20	736.0
21	844.5
22	953.0
23	1165.0
24	1377.0
25	1681.0
26	2324.5
27	2664.0
28	3354.0
29	4044.0
30	4788.0
31	5532.0
32	6052.0
33	6572.0
34	6840.0
35	7108.0
36	7982.0
37	8856.0
38	9683.0
39	10510.0
40	12764.5
41	15019.0
42	17103.0
43	19187.0
44	21495.0
45	23803.0
46	31153.0
47	38503.0
48	35534.0
49	32565.0
50	32579.0
51	32593.0
52	28428.0
53	24263.0
54	21895.5
55	19528.0
56	17790.5
57	16053.0
58	14964.0
59	13875.0
60	13445.5
61	13016.0
62	11723.5
63	10431.0
64	8874.5
65	7318.0
66	5934.5
67	4551.0
68	3485.5
69	2420.0
70	2042.0
71	1664.0
72	1401.0
73	1138.0
74	936.5
75	554.0
76	373.0
77	252.0
78	131.0
79	110.0
80	89.0
81	62.5
82	36.0
83	33.5
84	31.0
85	20.5
86	10.0
87	5.5
88	1.0
89	1.5
90	2.0
91	3.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	336764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.243062412681788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57336980132226	21.948409524000247
2	10.523655365752434	6.365351320720089
3	3.7163057726923894	3.371774022844367
4	1.835021928287224	2.2198673082332077
5	1.1503581945709769	1.7395177337675
6	0.8725116456947777	1.5832454493923316
7	0.6906133670389417	1.462038421367372
8	0.5526905765969098	1.3372044482337142
9	0.5416967311310923	1.4744311243508996
>10	7.308908108039902	47.87699720389965
>50	0.17190375968583782	3.264120891972329
>100	0.053969785017646765	3.0005489484775727
>500	0.00399776185315902	0.8197329251809385
>1k	0.004997202316448774	3.536760677559794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	3302	0.9805086054328848	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	3042	0.9033032034302955	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	2607	0.7741326270028863	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1695	0.5033198322861113	No Hit
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1055	0.3132757658181991	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	804	0.23874285850031476	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	736	0.2185506764380991	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCT	616	0.18291741397536554	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACATGTT	556	0.16510078274399878	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCT	500	0.14847192692805644	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCC	458	0.1360002850660997	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACATGTTCGTA	447	0.13273390267368246	No Hit
TCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	444	0.1318430711121141	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTC	414	0.12293475549643074	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.938877077122258E-4	0.0	0.0	0.23191314986162417	0.0
2	5.938877077122258E-4	0.0	0.0	1.3270420828829685	0.0
3	5.938877077122258E-4	0.0	0.0	1.7270254540271526	0.0
4	5.938877077122258E-4	0.0	0.0	2.343480894632443	0.0
5	5.938877077122258E-4	0.0	0.0	4.255502369611953	0.0
6	5.938877077122258E-4	0.0	0.0	5.107731230178998	0.0
7	5.938877077122258E-4	0.0	0.0	6.048449359195163	0.0
8	5.938877077122258E-4	0.0	0.0	7.340749011176967	0.0
9	5.938877077122258E-4	0.0	0.0	7.8497107766863445	0.0
10	5.938877077122258E-4	0.0	0.0	9.795286907151596	0.0
11	8.908315615683386E-4	0.0	0.0	11.104512358803197	0.0
12	8.908315615683386E-4	0.0	0.0	13.12877861054032	0.0
13	8.908315615683386E-4	0.0	0.0	13.632989274387999	0.0
14	8.908315615683386E-4	0.0	0.0	13.87202907674217	0.0
15	8.908315615683386E-4	0.0	0.0	14.325165397726598	0.0
16	0.0011877754154244516	0.0	0.0	14.99031963036429	0.0
17	0.0014847192692805644	0.0	0.0	15.761482818828616	0.0
18	0.0014847192692805644	0.0	0.0	16.70695204950648	0.0
19	0.0014847192692805644	0.0	0.0	17.39437707118338	0.0
20	0.0014847192692805644	0.0	0.0	17.965400102148685	0.0
21	0.0014847192692805644	0.0	0.0	18.586012756707962	0.0
22	0.0014847192692805644	0.0	0.0	19.216127614590633	0.0
23	0.0014847192692805644	0.0	0.0	19.877421577128196	0.0
24	0.0017816631231366772	0.0	0.0	20.405387749284365	0.0
25	0.0017816631231366772	0.0	0.0	20.87188654369232	0.0
26	0.0017816631231366772	0.0	0.0	21.299188749391266	0.0
27	0.0017816631231366772	0.0	0.0	21.749058687983275	0.0
28	0.0017816631231366772	0.0	0.0	22.19180197408274	0.0
29	0.0017816631231366772	0.0	0.0	22.619104179781687	0.0
30	0.0017816631231366772	0.0	0.0	23.136380373199035	0.0
31	0.0017816631231366772	0.0	0.0	23.601394448337707	0.0
32	0.0017816631231366772	0.0	0.0	24.0405744081909	0.0
33	0.0017816631231366772	0.0	0.0	24.470846052428406	0.0
34	0.0017816631231366772	0.0	0.0	24.910026012281598	0.0
35	0.0017816631231366772	0.0	0.0	25.35841123160433	0.0
36	0.0017816631231366772	0.0	0.0	25.816298654250453	0.0
37	0.0017816631231366772	0.0	0.0	26.278343290850565	0.0
38	0.0017816631231366772	0.0	0.0	26.694658573956836	0.0
39	0.0017816631231366772	0.0	0.0	27.166799301588057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACTA	35	1.2085002E-7	45.000004	38
CGACCCA	35	1.2085002E-7	45.000004	15
CGACAGG	35	1.2085002E-7	45.000004	2
CCGACCC	35	1.2085002E-7	45.000004	14
ACAACGA	20	7.026176E-4	45.0	13
CCTTAGT	20	7.026176E-4	45.0	37
GCGACGA	20	7.026176E-4	45.0	5
AGATAGT	25	3.884912E-5	45.0	41
CGGCCTA	20	7.026176E-4	45.0	45
CGATTAT	20	7.026176E-4	45.0	10
CCAGACG	20	7.026176E-4	45.0	21
TCTACGG	20	7.026176E-4	45.0	2
GTTGTCG	20	7.026176E-4	45.0	37
AACGAGC	20	7.026176E-4	45.0	15
GTTCACG	20	7.026176E-4	45.0	1
TTTGCGG	50	2.1827873E-11	45.0	2
CAATGCG	20	7.026176E-4	45.0	1
GGATTAC	20	7.026176E-4	45.0	8
CGGTTGG	30	2.1609158E-6	44.999996	2
GCTACGA	30	2.1609158E-6	44.999996	10
>>END_MODULE