SRR2935649.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935649.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495598
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	15274	3.0819333411353558	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	13409	2.705620280953515	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	13065	2.636209185670644	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	3624	0.7312378177474486	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	2970	0.5992760261340845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT	2463	0.49697537116776097	No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	2318	0.46771778740027203	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA	1964	0.39628892771964375	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	1873	0.3779272717000472	No Hit
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	1595	0.3218334214423787	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	1072	0.21630434343964264	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.2144883554816605	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	865	0.1745366204060549	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATG	709	0.14305949580103228	No Hit
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	551	0.1111788183164581	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATCTTCCC	538	0.10855572459937289	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.029397E-4	45.000004	1
ACGCCAA	20	7.029397E-4	45.000004	18
GGTAACC	20	7.029397E-4	45.000004	9
GCACGTC	20	7.029397E-4	45.000004	35
AAGCGTG	20	7.029397E-4	45.000004	1
TAACCTA	20	7.029397E-4	45.000004	11
ACAGCGT	20	7.029397E-4	45.000004	33
AATTCGG	20	7.029397E-4	45.000004	2
ACGTCCC	20	7.029397E-4	45.000004	37
GTATACG	20	7.029397E-4	45.000004	1
TTACGCG	20	7.029397E-4	45.000004	1
TCGACCA	20	7.029397E-4	45.000004	27
CTAAGCG	25	3.8875834E-5	45.0	1
TGCAACG	25	3.8875834E-5	45.0	1
CCGATGA	765	0.0	42.352943	18
TACGGCT	1530	0.0	41.76471	7
GATGAAT	820	0.0	41.707317	20
CGCTATC	65	0.0	41.538464	26
CTCGAAT	65	0.0	41.538464	43
CCCGCTA	60	3.6379788E-12	41.250004	24