##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935649.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495598
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960784748929576	33.0	31.0	34.0	30.0	34.0
2	31.9954095859951	33.0	31.0	34.0	30.0	34.0
3	32.043210424577985	33.0	31.0	34.0	30.0	34.0
4	35.72708122308807	37.0	35.0	37.0	35.0	37.0
5	35.7421720830189	37.0	35.0	37.0	35.0	37.0
6	35.64680446652327	37.0	35.0	37.0	33.0	37.0
7	35.875346551035314	37.0	35.0	37.0	35.0	37.0
8	35.559172958728645	37.0	35.0	37.0	33.0	37.0
9	37.35488642004205	39.0	37.0	39.0	34.0	39.0
10	37.12847913026283	39.0	37.0	39.0	34.0	39.0
11	37.16819075137511	39.0	37.0	39.0	34.0	39.0
12	37.02728622795088	39.0	37.0	39.0	33.0	39.0
13	36.83294928550963	39.0	35.0	39.0	33.0	39.0
14	37.97448738695475	40.0	37.0	41.0	33.0	41.0
15	37.91219698223156	40.0	37.0	41.0	33.0	41.0
16	37.929232967041834	40.0	36.0	41.0	33.0	41.0
17	37.804549251611185	40.0	36.0	41.0	33.0	41.0
18	37.80293907562177	40.0	36.0	41.0	33.0	41.0
19	37.703921323330604	40.0	36.0	41.0	33.0	41.0
20	37.62061792016917	40.0	36.0	41.0	32.0	41.0
21	37.819418157458266	40.0	36.0	41.0	33.0	41.0
22	37.88607702210259	40.0	36.0	41.0	33.0	41.0
23	37.86452931609894	40.0	36.0	41.0	33.0	41.0
24	37.763897352289554	40.0	36.0	41.0	33.0	41.0
25	37.62188104068217	39.0	36.0	41.0	33.0	41.0
26	37.50955411442338	39.0	35.0	41.0	33.0	41.0
27	37.462556346070805	40.0	36.0	41.0	33.0	41.0
28	37.12969180666589	39.0	35.0	41.0	31.0	41.0
29	36.92538912586411	39.0	35.0	41.0	31.0	41.0
30	36.90842578057216	39.0	35.0	41.0	31.0	41.0
31	36.846530857670935	39.0	35.0	41.0	31.0	41.0
32	36.743523985165396	39.0	35.0	41.0	31.0	41.0
33	36.640904523424226	39.0	35.0	41.0	30.0	41.0
34	36.27626826581221	39.0	35.0	41.0	30.0	41.0
35	36.38120008555321	39.0	35.0	41.0	30.0	41.0
36	36.075488601648914	39.0	35.0	41.0	29.0	41.0
37	36.31932332253157	39.0	35.0	41.0	30.0	41.0
38	36.28865330368565	39.0	35.0	41.0	30.0	41.0
39	36.25413339036881	39.0	35.0	41.0	30.0	41.0
40	36.24483351425954	39.0	35.0	41.0	30.0	41.0
41	36.197890225545706	39.0	35.0	41.0	30.0	41.0
42	36.25984971690765	39.0	35.0	41.0	30.0	41.0
43	36.198608549671306	39.0	35.0	41.0	30.0	41.0
44	36.1406543206389	39.0	35.0	41.0	30.0	41.0
45	36.20918365287996	39.0	35.0	40.0	30.0	41.0
46	36.07458464319872	38.0	35.0	40.0	30.0	41.0
47	35.887422870955895	38.0	35.0	40.0	30.0	41.0
48	35.82505780894999	38.0	35.0	40.0	29.0	41.0
49	35.949814163898964	38.0	35.0	40.0	29.0	41.0
50	35.81787658545838	38.0	35.0	40.0	29.0	41.0
51	34.54635208374529	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	12.0
11	11.0
12	11.0
13	5.0
14	12.0
15	20.0
16	32.0
17	73.0
18	136.0
19	284.0
20	619.0
21	1290.0
22	2187.0
23	2964.0
24	3394.0
25	3649.0
26	4166.0
27	4522.0
28	5197.0
29	6331.0
30	8348.0
31	10992.0
32	14670.0
33	19629.0
34	32178.0
35	43937.0
36	37676.0
37	56966.0
38	100267.0
39	135920.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.34382705337794	16.7627795108132	19.30112712319259	13.592266312616275
2	26.16556160436483	35.48480825184928	22.285804220356013	16.063825923429874
3	27.66758542205578	34.86959188697291	22.838671665341668	14.624151025629645
4	25.597762702835762	20.57272224665959	36.45071206905597	17.378802981448676
5	32.89278810648953	24.85724316885863	22.07293007639256	20.177038648259273
6	21.428052574869145	40.11275267454671	24.403649732242663	14.055545018341478
7	69.49241118810004	5.451595849862187	19.694792957195144	5.361200004842635
8	67.34672052752433	15.02508081146413	11.169334823788635	6.45886383722291
9	62.96776823151021	6.487516091671072	11.62070871956707	18.924006957251642
10	41.33148237079246	19.94116199016138	20.83664582988632	17.890709809159844
11	33.10667113265187	22.48556289573404	23.83262240767719	20.5751435639369
12	26.80902667080981	18.353584962005495	29.51908603343839	25.318302333746303
13	24.41051013119504	22.0317676826783	34.65570886081058	18.902013325316084
14	19.77792485038277	30.80238419041239	26.853215711120708	22.566475248084135
15	16.45587754591423	22.914741383137137	38.76428072752513	21.8651003434235
16	18.265812210703032	24.875403048438454	28.16940342777816	28.689381313080357
17	18.156449380344554	25.08464521648594	31.201296211849122	25.557609191320385
18	18.48695918869729	25.320320098144062	30.14883030197862	26.043890411180026
19	20.683497512096498	26.075771088664602	26.995871654042187	26.244859745196713
20	22.791254201994356	26.099782484997924	31.382289678328	19.72667363467972
21	21.003111392701342	31.327002933829434	28.062865467576543	19.607020205892677
22	19.256938082881693	22.32434352035319	30.404279274734762	28.014439122030353
23	20.56384408330946	28.90810697379731	28.6032227732961	21.924826169597132
24	22.16574723868942	24.42927534009419	26.994257442523985	26.41071997869241
25	18.656653174548726	33.15529925463783	25.471854204415678	22.716193366397768
26	18.13748239500563	25.440780632690203	29.672234351228212	26.749502621075955
27	23.122772892545974	27.497286106885017	25.88347814155828	23.49646285901073
28	17.26076376417984	25.980532609090435	31.99266340864975	24.766040218079976
29	21.232329428286636	25.720644554659224	28.982562480074574	24.06446353697957
30	18.709922154649533	27.675051150327484	32.80683134314505	20.808195351877934
31	22.60360211300288	23.334839930750327	23.797513307156205	30.264044649090593
32	23.241215662694362	26.845144653529672	28.171421192175917	21.742218491600045
33	20.64556354141865	21.200246974362287	27.741839151893267	30.412350332325794
34	20.267838046158378	24.942392826444014	26.59009923365308	28.199669893744527
35	18.463553121683297	21.372362277491032	30.098991521354	30.065093079471666
36	18.924410510131196	26.394577863510342	25.301554889244915	29.379456737113546
37	17.69639909765576	22.148999794188033	35.83468859842049	24.319912509735715
38	18.86529001327689	23.003119463758935	27.159714123140127	30.97187639982405
39	21.1142902110178	22.206707855963906	29.245073628222872	27.43392830479542
40	18.943175719030343	20.798510082768694	32.44706395102482	27.81125024717614
41	17.703461273047914	23.434921044879115	25.02734070758962	33.83427697448335
42	18.75976093527415	21.406664272252915	30.38107498416055	29.452499808312382
43	21.443791137171658	21.27228116336224	26.34070355409021	30.943224145375893
44	20.62155214508533	21.174419590070983	30.904483068938937	27.299545195904745
45	18.816258338411373	20.654038151889235	29.373201667480497	31.156501842218898
46	25.681903478222267	24.23900015738562	26.68029330223286	23.398803062159253
47	17.605397923316882	21.96134770519655	36.17084814708696	24.262406224399612
48	20.72062437701524	23.63730281397423	26.99970540639793	28.6423674026126
49	19.92885362733506	19.365897360360616	34.79957546237071	25.905673549933617
50	22.114697799426146	19.398786920044067	30.415982308241762	28.070532972288024
51	20.09794228386717	20.524497677553178	27.256970367112054	32.120589671467606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	309.0
1	583.5
2	858.0
3	1460.5
4	2063.0
5	1471.5
6	880.0
7	869.5
8	859.0
9	877.0
10	895.0
11	872.0
12	849.0
13	937.5
14	1026.0
15	979.5
16	933.0
17	974.0
18	1015.0
19	1008.5
20	1002.0
21	1044.0
22	1086.0
23	1426.0
24	1766.0
25	2134.5
26	2919.5
27	3336.0
28	4042.0
29	4748.0
30	5234.5
31	5721.0
32	6285.5
33	6850.0
34	7537.0
35	8224.0
36	9593.5
37	10963.0
38	11860.5
39	12758.0
40	15988.5
41	19219.0
42	21476.5
43	23734.0
44	27265.0
45	30796.0
46	61802.0
47	92808.0
48	72562.5
49	52317.0
50	49852.0
51	47387.0
52	40342.0
53	33297.0
54	29564.5
55	25832.0
56	23932.5
57	22033.0
58	20194.0
59	18355.0
60	17446.5
61	16538.0
62	14233.5
63	11929.0
64	11184.0
65	10439.0
66	8964.0
67	7489.0
68	6067.0
69	4645.0
70	4230.0
71	3815.0
72	3354.5
73	2894.0
74	2401.5
75	1342.5
76	776.0
77	566.5
78	357.0
79	260.5
80	164.0
81	128.5
82	93.0
83	81.5
84	70.0
85	46.0
86	22.0
87	16.5
88	11.0
89	9.5
90	8.0
91	7.5
92	7.0
93	7.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.104230112721496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.47896528321967	20.650697487642795
2	10.38246480229099	5.83582359881635
3	3.6353005164217405	3.065019667272356
4	1.8983591084696296	2.1340768488404516
5	1.139372689731311	1.6010596128179597
6	0.7960332360447643	1.3423140745905842
7	0.6285870950664426	1.2366169455944114
8	0.5289547369233127	1.1892692516565473
9	0.47417350028780747	1.1993653048898838
>10	6.78022205257029	42.84096617258604
>50	0.19425442548320931	3.428080636736877
>100	0.05180118012885582	2.6668016702797375
>500	0.00287784334049199	0.5384557470585292
>1k	0.006475147516106977	3.83005210694054
>5k	0.0	0.0
>10k+	0.0021583825053689927	8.441400874276935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	15274	3.0819333411353558	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	13409	2.705620280953515	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	13065	2.636209185670644	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	3624	0.7312378177474486	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCT	2970	0.5992760261340845	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCTTCCCT	2463	0.49697537116776097	No Hit
GAACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCT	2318	0.46771778740027203	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTA	1964	0.39628892771964375	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTC	1873	0.3779272717000472	No Hit
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	1595	0.3218334214423787	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	1072	0.21630434343964264	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.2144883554816605	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTT	865	0.1745366204060549	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATG	709	0.14305949580103228	No Hit
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	551	0.1111788183164581	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATCTTCCC	538	0.10855572459937289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22881448270574134	0.0
2	0.0	0.0	0.0	1.549441281038261	0.0
3	0.0	0.0	0.0	1.9792250977606851	0.0
4	0.0	0.0	0.0	2.9396809510934263	0.0
5	0.0	0.0	0.0	6.907614639284259	0.0
6	0.0	0.0	0.0	7.943938433972695	0.0
7	0.0	0.0	0.0	9.381999120254722	0.0
8	0.0	0.0	0.0	10.908236110718768	0.0
9	0.0	0.0	0.0	11.435074394973345	0.0
10	0.0	0.0	0.0	15.224637710402382	0.0
11	0.0	0.0	0.0	16.531745487269923	0.0
12	0.0	0.0	0.0	20.78922836653901	0.0
13	0.0	0.0	0.0	21.420788623037218	0.0
14	0.0	0.0	0.0	21.698836557048253	0.0
15	0.0	0.0	0.0	22.442786290501576	0.0
16	0.0	0.0	0.0	23.177454307725213	0.0
17	0.0	0.0	0.0	23.91595607730459	0.0
18	0.0	0.0	0.0	24.784401874099572	0.0
19	2.0177643977578602E-4	0.0	0.0	25.814067046275408	0.0
20	2.0177643977578602E-4	0.0	0.0	26.42585321167559	0.0
21	2.0177643977578602E-4	0.0	0.0	26.98820414933071	0.0
22	2.0177643977578602E-4	0.0	0.0	27.649223766036183	0.0
23	2.0177643977578602E-4	0.0	0.0	28.197853905786545	0.0
24	2.0177643977578602E-4	0.0	0.0	28.65346510680027	0.0
25	2.0177643977578602E-4	0.0	0.0	29.039059883211795	0.0
26	2.0177643977578602E-4	0.0	0.0	29.410732085278795	0.0
27	2.0177643977578602E-4	0.0	0.0	29.910734103043193	0.0
28	2.0177643977578602E-4	0.0	0.0	30.28523117526705	0.0
29	2.0177643977578602E-4	0.0	0.0	30.674457927594542	0.0
30	2.0177643977578602E-4	0.0	0.0	31.131481563686698	0.0
31	4.0355287955157204E-4	0.0	0.0	31.53543799611782	0.0
32	4.0355287955157204E-4	0.0	0.0	31.941210416506927	0.0
33	4.0355287955157204E-4	0.0	0.0	32.301179585066926	0.0
34	6.053293193273581E-4	0.0	0.0	32.68516014996025	0.0
35	6.053293193273581E-4	0.0	0.0	33.08124730124012	0.0
36	6.053293193273581E-4	0.0	0.0	33.44726976299339	0.0
37	6.053293193273581E-4	0.0	0.0	33.83165388076627	0.0
38	8.071057591031441E-4	0.0	0.0	34.18012179225905	0.0
39	8.071057591031441E-4	0.0	0.0	34.56934854458654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.029397E-4	45.000004	1
ACGCCAA	20	7.029397E-4	45.000004	18
GGTAACC	20	7.029397E-4	45.000004	9
GCACGTC	20	7.029397E-4	45.000004	35
AAGCGTG	20	7.029397E-4	45.000004	1
TAACCTA	20	7.029397E-4	45.000004	11
ACAGCGT	20	7.029397E-4	45.000004	33
AATTCGG	20	7.029397E-4	45.000004	2
ACGTCCC	20	7.029397E-4	45.000004	37
GTATACG	20	7.029397E-4	45.000004	1
TTACGCG	20	7.029397E-4	45.000004	1
TCGACCA	20	7.029397E-4	45.000004	27
CTAAGCG	25	3.8875834E-5	45.0	1
TGCAACG	25	3.8875834E-5	45.0	1
CCGATGA	765	0.0	42.352943	18
TACGGCT	1530	0.0	41.76471	7
GATGAAT	820	0.0	41.707317	20
CGCTATC	65	0.0	41.538464	26
CTCGAAT	65	0.0	41.538464	43
CCCGCTA	60	3.6379788E-12	41.250004	24
>>END_MODULE