##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935648.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	822905
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.137216325092204	33.0	31.0	34.0	30.0	34.0
2	32.164461268311655	33.0	31.0	34.0	30.0	34.0
3	32.16483190647766	33.0	31.0	34.0	30.0	34.0
4	35.82625697984579	37.0	35.0	37.0	35.0	37.0
5	35.86714383798859	37.0	35.0	37.0	35.0	37.0
6	35.7815179151907	37.0	35.0	37.0	35.0	37.0
7	36.035731949617514	37.0	35.0	37.0	35.0	37.0
8	35.82552056434218	37.0	35.0	37.0	35.0	37.0
9	37.67970178817725	39.0	38.0	39.0	35.0	39.0
10	37.33402762165742	39.0	37.0	39.0	34.0	39.0
11	37.28790686652773	39.0	37.0	39.0	34.0	39.0
12	37.15808386144209	39.0	37.0	39.0	34.0	39.0
13	37.08287347871261	39.0	37.0	39.0	33.0	39.0
14	38.16546502937763	40.0	37.0	41.0	33.0	41.0
15	38.27205327467934	40.0	38.0	41.0	33.0	41.0
16	38.146764207290026	40.0	37.0	41.0	33.0	41.0
17	38.15489880362861	40.0	37.0	41.0	33.0	41.0
18	38.05482771401316	40.0	37.0	41.0	33.0	41.0
19	37.88964582789022	40.0	37.0	41.0	33.0	41.0
20	37.92559408437183	40.0	36.0	41.0	33.0	41.0
21	37.904506595536546	40.0	36.0	41.0	33.0	41.0
22	38.01984797759158	40.0	36.0	41.0	34.0	41.0
23	38.03174120949563	40.0	36.0	41.0	34.0	41.0
24	37.96835965269381	40.0	36.0	41.0	34.0	41.0
25	37.74678365060365	40.0	35.0	41.0	33.0	41.0
26	37.77425219192981	40.0	36.0	41.0	33.0	41.0
27	37.769424174114874	40.0	36.0	41.0	33.0	41.0
28	37.638550014886285	40.0	35.0	41.0	33.0	41.0
29	37.5815944732381	40.0	35.0	41.0	33.0	41.0
30	37.37309166914771	40.0	35.0	41.0	33.0	41.0
31	37.316443574896255	40.0	35.0	41.0	33.0	41.0
32	37.161583657894894	40.0	35.0	41.0	32.0	41.0
33	37.013943286284565	40.0	35.0	41.0	32.0	41.0
34	36.88778534581756	40.0	35.0	41.0	31.0	41.0
35	36.73403491290003	40.0	35.0	41.0	31.0	41.0
36	36.61841281800451	39.0	35.0	41.0	31.0	41.0
37	36.577054459506265	39.0	35.0	41.0	31.0	41.0
38	36.43597742145205	39.0	35.0	41.0	30.0	41.0
39	36.34793445172894	39.0	35.0	41.0	30.0	41.0
40	36.208739769475216	39.0	35.0	41.0	30.0	41.0
41	36.077138916399825	39.0	35.0	41.0	29.0	41.0
42	36.11712530608029	39.0	35.0	41.0	30.0	41.0
43	36.068063749764555	39.0	35.0	41.0	30.0	41.0
44	35.985811241880896	39.0	35.0	41.0	30.0	41.0
45	35.969071764055386	39.0	35.0	41.0	29.0	41.0
46	35.82154440670551	38.0	35.0	41.0	29.0	41.0
47	35.6777769001282	38.0	35.0	40.0	28.0	41.0
48	35.59222753537772	38.0	35.0	40.0	28.0	41.0
49	35.57610173713855	38.0	35.0	40.0	28.0	41.0
50	35.444011155601196	38.0	35.0	40.0	28.0	41.0
51	34.28363541356536	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	17.0
10	18.0
11	21.0
12	15.0
13	30.0
14	25.0
15	59.0
16	59.0
17	148.0
18	293.0
19	599.0
20	1153.0
21	1927.0
22	2775.0
23	3666.0
24	4756.0
25	6684.0
26	8611.0
27	9808.0
28	10458.0
29	10868.0
30	12955.0
31	16072.0
32	20651.0
33	29127.0
34	52821.0
35	62244.0
36	58538.0
37	87105.0
38	150977.0
39	270230.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.7716261293831	17.226654352568037	20.325067899696805	16.676651618352057
2	30.910372400216307	27.333410296449774	23.99086164259544	17.765355660738482
3	30.569992891038456	26.731882781122973	26.52298868034585	16.175135647492723
4	26.903713065299158	24.05174351838912	30.400836062485947	18.643707353825775
5	26.472800627046865	27.870531835388046	26.103863750979762	19.55280378658533
6	24.53175032354889	35.31367533311865	25.864103389820208	14.290470953512251
7	77.28206779640419	5.385797874602779	12.94803166829707	4.384102660695949
8	77.95079626445337	7.64985022572472	9.91305193187549	4.4863015779464215
9	72.5126229637686	6.4420558873746065	11.5432522587662	9.502068890090595
10	42.13876449893973	23.28822889640967	19.261275602894624	15.311731001755973
11	32.254999058214494	24.463212643014685	24.749636956878376	18.53215134189244
12	27.039937781396397	22.304883309738063	29.053414428153918	21.601764480711623
13	26.136917384145193	22.75912772434242	31.3969413237251	19.70701356778729
14	21.05735169916333	26.713533153887752	30.196802790115505	22.032312356833415
15	20.587309592237258	24.50598793299348	33.59063318366032	21.31606929110894
16	24.374867086723253	25.196104046032048	28.295975841682818	22.133053025561882
17	24.525674288040538	25.677326058293488	28.290021326884634	21.506978326781343
18	25.212509341904592	24.574161051397184	28.80915780071819	21.404171805980035
19	24.76045230008324	26.242275839860007	26.31871236655507	22.67855949350168
20	25.748901756581866	26.434643124054418	28.628821066830312	19.187634052533404
21	24.9913416494006	26.93956167479843	29.422108262800688	18.646988413000283
22	24.312526962407567	22.783553387085995	30.346880867171787	22.557038783334647
23	23.72813386721432	25.27825204610496	30.024607943808824	20.969006142871898
24	23.807000808112722	25.050765276672276	29.142732150126687	21.999501765088315
25	23.457021162831676	28.177250107849634	26.734070153905982	21.631658575412715
26	23.1631840856478	24.64962541241091	28.17360448654462	24.013586015396672
27	23.90859212181236	25.103869827015266	28.131922882957326	22.855615168215042
28	22.015785540250697	26.35516857960518	28.887174096645417	22.741871783498702
29	24.42614882641374	24.784634921406482	28.38954678851143	22.399669463668346
30	24.6684611224868	25.1033837441746	29.903087233641795	20.325067899696805
31	26.230123768843306	24.042629465126595	27.12633900632515	22.600907759704945
32	26.53988005905907	25.37960031838426	26.068501224321157	22.01201839823552
33	24.272060565921947	25.510356602523988	26.917566426258194	23.300016405295874
34	23.855487571469368	24.880514761728268	29.548732842794735	21.715264824007633
35	22.847351759923683	27.042732757730235	28.090970403631037	22.018945078715042
36	24.856575181825363	27.143716467879038	26.11929688117097	21.880411469124624
37	22.191869049282726	26.266093899052745	28.38249858732174	23.159538464342784
38	24.04785485566378	27.247495154361683	25.80808234243321	22.896567647541332
39	23.059283878454988	24.996323998517447	27.145539278531544	24.79885284449602
40	23.697753689672563	25.88123780995376	28.43742594831724	21.983582552056436
41	22.04106184796544	25.65678905827526	27.823989403394073	24.47815969036523
42	22.54634496083995	25.868599656096393	28.643403552050355	22.941651831013303
43	23.88769055966363	24.11444820483531	27.5323396989932	24.465521536507858
44	23.51255612737801	24.337195666571475	28.03555695979487	24.114691246255642
45	22.916132481878222	24.1893049622982	27.304852929560518	25.58970962626306
46	24.56990782654134	25.093540566651072	26.8523098048985	23.48424180190909
47	21.435767190623462	25.553861016763786	30.584210814128	22.42616097848476
48	22.35774481866072	25.800912620533357	28.233271155236633	23.608071405569294
49	23.261980423013593	24.372072110389414	29.701484375474692	22.664463091122304
50	22.058439309519326	24.72423912845347	29.723236582594588	23.49408497943262
51	22.071198984086863	24.173264228556153	27.97589029110286	25.77964649625412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	443.0
1	894.0
2	1345.0
3	5284.0
4	9223.0
5	6035.0
6	2847.0
7	2797.0
8	2747.0
9	2759.0
10	2771.0
11	2753.0
12	2735.0
13	2622.0
14	2509.0
15	2522.5
16	2536.0
17	2561.0
18	2586.0
19	2484.5
20	2383.0
21	2569.5
22	2756.0
23	2960.0
24	3164.0
25	3513.5
26	5130.5
27	6398.0
28	7481.0
29	8564.0
30	9831.5
31	11099.0
32	12984.5
33	14870.0
34	17748.0
35	20626.0
36	22377.0
37	24128.0
38	25772.5
39	27417.0
40	29257.5
41	31098.0
42	34872.0
43	38646.0
44	42067.5
45	45489.0
46	64811.0
47	84133.0
48	74518.5
49	64904.0
50	64786.0
51	64668.0
52	60257.0
53	55846.0
54	53892.0
55	51938.0
56	48802.5
57	45667.0
58	44128.0
59	42589.0
60	39139.5
61	35690.0
62	32317.5
63	28945.0
64	25778.0
65	22611.0
66	20277.0
67	17943.0
68	15355.0
69	12767.0
70	10778.0
71	8789.0
72	7822.0
73	6855.0
74	5509.0
75	3395.5
76	2628.0
77	1973.5
78	1319.0
79	964.5
80	610.0
81	487.5
82	365.0
83	227.0
84	89.0
85	72.5
86	56.0
87	39.0
88	22.0
89	32.5
90	43.0
91	25.5
92	8.0
93	5.5
94	3.0
95	7.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	822905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.906313869547894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89502632081208	20.063251701679885
2	7.952487628260823	4.438492315958861
3	3.333157540905904	2.790484215395117
4	2.0195323195074493	2.254308111114838
5	1.479611767268556	2.064525519123638
6	1.1753425850685009	1.9679687449900427
7	0.989875059321511	1.9336634867944398
8	0.8915425395943484	1.990373275037897
9	0.7915378262352816	1.9880012716687285
>10	9.32759207846482	52.27830958094945
>50	0.10931388989862319	1.9085446757470235
>100	0.02842161128798996	1.4590159599914028
>500	0.002623533349660612	0.5100515775498925
>1k	0.0021862777913838434	1.0918520373005831
>5k	0.001311766674830306	2.0153138408645987
>10k+	4.372555582767687E-4	1.245843685833589
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC	10210	1.2407264508053784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5669	0.6889009059368942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG	5576	0.6775994798913605	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC	5271	0.6405356632904162	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCT	3232	0.3927549352598416	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	2080	0.25276307714742285	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTT	1394	0.16939986997284012	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCT	1164	0.14145010663442317	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTC	1078	0.13099932556005858	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	912	0.11082688767233156	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCATACT	854	0.10377868648264381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4304142033406044E-4	0.0	0.0	0.06525662135969523	0.0
2	2.4304142033406044E-4	0.0	0.0	0.4473177341248382	0.0
3	2.4304142033406044E-4	0.0	0.0	0.635431793463401	0.0
4	2.4304142033406044E-4	0.0	0.0	1.2293035040496776	0.0
5	2.4304142033406044E-4	0.0	0.0	2.959879937538355	0.0
6	2.4304142033406044E-4	0.0	0.0	3.7011562695572393	0.0
7	2.4304142033406044E-4	0.0	0.0	4.2846987197793185	0.0
8	2.4304142033406044E-4	0.0	0.0	5.074097253024346	0.0
9	2.4304142033406044E-4	0.0	0.0	5.4409682770186105	0.0
10	2.4304142033406044E-4	0.0	0.0	6.658605792892254	0.0
11	2.4304142033406044E-4	0.0	0.0	7.193053876206853	0.0
12	2.4304142033406044E-4	0.0	0.0	8.328057309166915	0.0
13	2.4304142033406044E-4	0.0	0.0	8.585438173300684	0.0
14	2.4304142033406044E-4	0.0	0.0	8.726645238514774	0.0
15	2.4304142033406044E-4	0.0	0.0	8.954496570077955	0.0
16	2.4304142033406044E-4	0.0	0.0	9.196201262600178	0.0
17	2.4304142033406044E-4	0.0	0.0	9.467921570533658	0.0
18	2.4304142033406044E-4	0.0	0.0	9.768320766066557	0.0
19	4.860828406681209E-4	0.0	0.0	10.071757979353631	0.0
20	4.860828406681209E-4	0.0	0.0	10.301067559438817	0.0
21	4.860828406681209E-4	0.0	0.0	10.538397506395027	0.0
22	4.860828406681209E-4	0.0	0.0	10.843414488914274	0.0
23	4.860828406681209E-4	0.0	0.0	11.097878856004035	0.0
24	6.076035508351511E-4	0.0	0.0	11.309932495245501	0.0
25	6.076035508351511E-4	0.0	0.0	11.499626323816237	0.0
26	6.076035508351511E-4	0.0	0.0	11.708398903883195	0.0
27	6.076035508351511E-4	0.0	0.0	11.98109137749801	0.0
28	6.076035508351511E-4	0.0	0.0	12.188648750463297	0.0
29	6.076035508351511E-4	0.0	0.0	12.423305241795832	0.0
30	7.291242610021813E-4	0.0	0.0	12.738165401838609	0.0
31	8.506449711692115E-4	0.0	0.0	12.961885029256111	0.0
32	8.506449711692115E-4	0.0	0.0	13.197392165559815	0.0
33	8.506449711692115E-4	0.0	0.0	13.419288982324813	0.0
34	8.506449711692115E-4	0.0	0.0	13.655282201469186	0.0
35	9.721656813362418E-4	0.0	0.0	13.905493343703101	0.0
36	0.001093686391503272	0.0	0.0	14.12568887052576	0.0
37	0.0012152071016703022	0.0	0.0	14.366056835236146	0.0
38	0.0012152071016703022	0.0	0.0	14.667549717160547	0.0
39	0.0012152071016703022	0.0	0.0	15.199810427692139	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	30	2.1647465E-6	45.000004	1
TCCGCAC	25	3.889836E-5	45.0	36
CGCGTAA	20	7.0321176E-4	45.0	31
TGCCGTA	20	7.0321176E-4	45.0	32
CTCGAAT	80	0.0	42.1875	43
CGTTTTT	2665	0.0	41.538464	1
CGAATGC	65	0.0	41.53846	45
GCGAATG	60	3.6379788E-12	41.250004	1
TACGGCT	620	0.0	40.64516	7
CGTTAGG	140	0.0	40.17857	2
GCCGATT	45	1.9272193E-8	40.0	9
ATACGGT	40	3.4566256E-7	39.375	28
CCGATGA	545	0.0	39.220184	18
GCGTTAG	70	0.0	38.571426	1
GCGAGAC	165	0.0	38.181816	21
CGATGAA	580	0.0	38.01724	19
TCGTTTA	30	1.1396398E-4	37.500004	38
ATTACGG	30	1.1396398E-4	37.500004	2
TACGGGT	90	0.0	37.5	4
CCGATTA	85	0.0	37.058823	18
>>END_MODULE