SRR2935647.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935647.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261415
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	7369	2.8188895051928924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	7016	2.6838551728095172	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	5411	2.0698888740125856	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	2315	0.8855650976416808	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	1427	0.545875332325995	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1415	0.5412849300919993	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT	1412	0.5401373295335004	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTA	1141	0.4364707457490962	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT	942	0.36034657536866666	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC	872	0.33356922900369146	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	774	0.2960809440927261	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.23334544689478415	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	495	0.18935409215232485	No Hit
AATGAAGATGGAAGTAGAAGCAATAAAAATAAAACACAAGCCGAGCATGGT	425	0.16257674578734962	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	400	0.15301340779985848	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATG	340	0.1300613966298797	No Hit
ACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	333	0.12738366199338216	TruSeq Adapter, Index 15 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	306	0.11705525696689173	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTGAGTG	296	0.11322992177189527	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTAT	293	0.11208232121339631	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	275	0.1051967178624027	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT	263	0.10060631562840694	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.023277E-4	45.000004	6
CGGGTGA	30	2.1590458E-6	45.000004	6
GAGTGCG	20	7.023277E-4	45.000004	1
GTCGAAT	30	2.1590458E-6	45.000004	43
GGTAACG	20	7.023277E-4	45.000004	1
ACGCACG	20	7.023277E-4	45.000004	1
TGGACCA	20	7.023277E-4	45.000004	22
GGCGTAG	20	7.023277E-4	45.000004	1
GGCAACG	20	7.023277E-4	45.000004	1
ATAGCGG	35	1.2071177E-7	45.000004	2
CGCGAGG	20	7.023277E-4	45.000004	2
ATCACGC	20	7.023277E-4	45.000004	11
AGAACGA	20	7.023277E-4	45.000004	32
CCGTCGA	20	7.023277E-4	45.000004	41
ACGGCTA	20	7.023277E-4	45.000004	30
CTAGTAG	20	7.023277E-4	45.000004	1
GTGTCCA	35	1.2071177E-7	45.000004	9
TCTCGCG	20	7.023277E-4	45.000004	32
AGTCTTC	20	7.023277E-4	45.000004	11
GTCGTAA	20	7.023277E-4	45.000004	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content