##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935647.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261415
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.946433831264464	33.0	31.0	34.0	30.0	34.0
2	31.96466537880382	33.0	31.0	34.0	30.0	34.0
3	31.991928542738556	33.0	31.0	34.0	30.0	34.0
4	35.70005164202513	37.0	35.0	37.0	35.0	37.0
5	35.72593768528967	37.0	35.0	37.0	35.0	37.0
6	35.66520283839871	37.0	35.0	37.0	35.0	37.0
7	35.91000133886732	37.0	35.0	37.0	35.0	37.0
8	35.639358873821315	37.0	35.0	37.0	33.0	37.0
9	37.45277432435017	39.0	37.0	39.0	35.0	39.0
10	37.15419161103992	39.0	37.0	39.0	34.0	39.0
11	37.15065700131974	39.0	37.0	39.0	34.0	39.0
12	36.95708738978253	39.0	35.0	39.0	33.0	39.0
13	36.702802058030336	39.0	35.0	39.0	33.0	39.0
14	37.81359906661821	40.0	36.0	41.0	33.0	41.0
15	37.8105502744678	40.0	36.0	41.0	33.0	41.0
16	37.851523439741406	40.0	36.0	41.0	33.0	41.0
17	37.774936403802386	40.0	36.0	41.0	33.0	41.0
18	37.77495935581355	39.0	36.0	41.0	33.0	41.0
19	37.627477382705656	39.0	36.0	41.0	33.0	41.0
20	37.62923703689536	39.0	35.0	41.0	33.0	41.0
21	37.76799724575866	39.0	36.0	41.0	33.0	41.0
22	37.87020637683377	40.0	36.0	41.0	33.0	41.0
23	37.855077176137556	40.0	36.0	41.0	34.0	41.0
24	37.78508501807471	40.0	35.0	41.0	33.0	41.0
25	37.666044412141616	39.0	35.0	41.0	33.0	41.0
26	37.64354761585984	39.0	35.0	41.0	33.0	41.0
27	37.63507832373812	40.0	35.0	41.0	33.0	41.0
28	37.50834114339269	39.0	35.0	41.0	33.0	41.0
29	37.47211904443127	39.0	35.0	41.0	33.0	41.0
30	37.38804965285083	39.0	36.0	41.0	33.0	41.0
31	37.302082895013676	39.0	35.0	41.0	33.0	41.0
32	37.19610580877149	39.0	35.0	41.0	33.0	41.0
33	37.08669357152421	39.0	35.0	41.0	32.0	41.0
34	36.952894822408815	39.0	35.0	41.0	32.0	41.0
35	36.91466824780522	39.0	35.0	41.0	32.0	41.0
36	36.734797161601286	39.0	35.0	41.0	31.0	41.0
37	36.761846106765105	39.0	35.0	41.0	31.0	41.0
38	36.51881491115659	39.0	35.0	41.0	30.0	41.0
39	36.649373601361816	39.0	35.0	41.0	31.0	41.0
40	36.46311803071744	39.0	35.0	41.0	30.0	41.0
41	36.375311286651495	39.0	35.0	41.0	30.0	41.0
42	36.385318363521606	39.0	35.0	41.0	30.0	41.0
43	36.42214486544383	39.0	35.0	41.0	31.0	41.0
44	36.396297075531244	39.0	35.0	40.0	31.0	41.0
45	36.438716217508556	39.0	35.0	40.0	31.0	41.0
46	36.37892622841076	39.0	35.0	40.0	31.0	41.0
47	36.19566589522407	38.0	35.0	40.0	30.0	41.0
48	36.175839182908405	38.0	35.0	40.0	30.0	41.0
49	36.18115257349425	38.0	35.0	40.0	30.0	41.0
50	36.01741675114282	38.0	35.0	40.0	30.0	41.0
51	34.685530669624924	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	8.0
11	4.0
12	8.0
13	0.0
14	6.0
15	9.0
16	11.0
17	21.0
18	78.0
19	168.0
20	355.0
21	618.0
22	800.0
23	1064.0
24	1325.0
25	1681.0
26	2002.0
27	2317.0
28	2498.0
29	2901.0
30	3830.0
31	5373.0
32	7292.0
33	10126.0
34	18138.0
35	26565.0
36	18524.0
37	26366.0
38	50244.0
39	79023.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.615802459690535	17.453091827171356	20.22454717594629	13.706558537191821
2	26.793030239274717	33.8415928695752	22.9168180861848	16.448558804965284
3	26.589139873381406	34.07073044775549	24.718550963028136	14.621578715834977
4	25.190979859610195	22.263833368398906	35.79480902014039	16.750377751850507
5	30.46611709351032	26.318306141575654	24.536847541265804	18.678729223648222
6	21.280339689765317	39.036015530860894	24.93276973394794	14.750875045425854
7	70.62639863818066	5.583841784136335	18.94918042193447	4.8405791557485225
8	69.34529388137636	13.369546506512634	10.759520302966548	6.525639309144464
9	65.04561712220033	6.049002543847905	12.298452651913625	16.60692768203814
10	41.16060669816193	21.21339632385288	21.148365625537938	16.477631352447258
11	33.363808503720136	24.25032993516057	23.93741751620986	18.448444044909433
12	27.354971979419695	19.98852399441501	29.52278943442419	23.1337145917411
13	23.606526021842665	25.591874988045827	34.71797716274889	16.083621827362624
14	18.570089704110323	33.02832660711895	26.970143258803052	21.431440429967676
15	15.204177266032936	23.7824914408125	42.587839259415105	18.425492033739456
16	16.73469387755102	24.347110915593976	31.542183883862823	27.376011322992177
17	17.312702025514987	24.533022206070807	31.352064724671497	26.80221104374271
18	19.51992043302794	25.065126331694813	31.7896830709791	23.625270164298147
19	20.267008396610752	27.276935141441772	28.093644205573515	24.362412256373965
20	22.318153128167857	25.145840904309242	33.19472868810129	19.34127727942161
21	20.74441022894631	31.1344031520762	28.27190482566035	19.84928179331714
22	17.800814796396534	24.977143622209898	30.344471434309433	26.87757014708414
23	17.33794923780196	30.523497121435266	29.05265573895913	23.085897901803644
24	21.01715662834956	24.202130711703614	28.650230476445497	26.13048218350133
25	16.06832048658264	32.821375973069635	27.235238987816306	23.875064552531416
26	16.977220128913796	28.060363789377046	30.645525314155652	24.316890767553506
27	21.757358988581373	29.22250062161697	26.77428609681923	22.245854292982422
28	16.041543140217662	27.18780483139835	35.51594208442515	21.254709943958837
29	21.442533902033166	26.11709351031884	29.2970946579194	23.143277929728594
30	21.060765449572518	29.82881625002391	29.68727884780904	19.423139452594533
31	24.40602107759692	25.365797678021533	26.974351127517547	23.25383011686399
32	27.880955568731707	29.384312300365316	24.76407245184859	17.970659679054375
33	24.859323298204007	26.50153969741599	24.448864831780885	24.190272172599123
34	25.657288219880265	26.668706845437328	27.28764607998776	20.38635885469464
35	23.98867700782281	24.200600577625615	28.903850199873766	22.90687221467781
36	26.903199892890616	27.21113937608783	28.328137253026796	17.55752347799476
37	28.671652353537482	23.25383011686399	30.438574680106345	17.635942849492185
38	21.409636019356196	28.57793164126007	27.74286096819234	22.2695713711914
39	30.00937207122774	24.026547826253275	27.462463898399097	18.501616204119887
40	23.229730505135514	26.324809211407153	30.531912858864256	19.91354742459308
41	26.078840158368877	23.86703134862192	26.18441940975078	23.86970908325842
42	23.238911309603505	22.135302105847025	33.53288831933898	21.09289826521049
43	28.73591798481342	22.63756861695006	26.22496796281774	22.40154543541878
44	23.73926515310904	23.880037488284913	29.17774419983551	23.202953158770537
45	20.860317885354704	23.052617485607175	28.4352466384867	27.651817990551418
46	28.656733546276993	25.764397605340168	26.58454947114741	18.99431937723543
47	20.16257674578735	23.608821222959662	35.28986477440086	20.938737256852132
48	24.481762714457854	23.323833750932426	28.087141135742023	24.1072623988677
49	22.014804047204635	19.962129181569534	35.136086299561995	22.88698047166383
50	24.126389074842685	21.355316259587244	30.007841937149742	24.51045272842033
51	21.322418376910278	22.567182449362125	26.44415966949104	29.666239504236557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	297.0
1	346.5
2	396.0
3	739.5
4	1083.0
5	747.0
6	411.0
7	455.5
8	500.0
9	531.5
10	563.0
11	638.0
12	713.0
13	674.0
14	635.0
15	624.5
16	614.0
17	605.5
18	597.0
19	563.0
20	529.0
21	677.5
22	826.0
23	981.5
24	1137.0
25	1204.5
26	1538.5
27	1805.0
28	2131.0
29	2457.0
30	2716.0
31	2975.0
32	3616.5
33	4258.0
34	4883.0
35	5508.0
36	5788.0
37	6068.0
38	7128.0
39	8188.0
40	9495.5
41	10803.0
42	12903.0
43	15003.0
44	17645.5
45	20288.0
46	34510.5
47	48733.0
48	39725.0
49	30717.0
50	29641.0
51	28565.0
52	23981.5
53	19398.0
54	16377.0
55	13356.0
56	11432.5
57	9509.0
58	8368.0
59	7227.0
60	6785.5
61	6344.0
62	5300.0
63	4256.0
64	3347.5
65	2439.0
66	1870.5
67	1302.0
68	1142.0
69	982.0
70	800.5
71	619.0
72	534.0
73	449.0
74	349.0
75	190.0
76	131.0
77	111.0
78	91.0
79	66.5
80	42.0
81	42.5
82	43.0
83	37.0
84	31.0
85	17.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	261415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.99592601801733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05835019993089	22.955071438134766
2	11.06037419163746	6.856530803511658
3	3.8813743397344127	3.6092037564791615
4	1.8697240460087872	2.3181531281678556
5	1.0885126129239275	1.6869728209934396
6	0.7503579009725033	1.3954822791347092
7	0.6392851853680209	1.3870665417057169
8	0.47144197067680305	1.1690224355909187
9	0.38381793947771137	1.0707113210795096
>10	5.500567704990867	38.612933458294286
>50	0.23818926790739003	4.59499263622975
>100	0.04319494495729871	2.5985501979610963
>500	0.004936565137976996	1.2233421953598684
>1k	0.006170706422471244	2.9493334353422718
>5k	0.0037024238534827465	7.572633552014995
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	7369	2.8188895051928924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	7016	2.6838551728095172	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	5411	2.0698888740125856	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	2315	0.8855650976416808	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	1427	0.545875332325995	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	1415	0.5412849300919993	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT	1412	0.5401373295335004	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTA	1141	0.4364707457490962	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT	942	0.36034657536866666	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC	872	0.33356922900369146	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	774	0.2960809440927261	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	610	0.23334544689478415	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	495	0.18935409215232485	No Hit
AATGAAGATGGAAGTAGAAGCAATAAAAATAAAACACAAGCCGAGCATGGT	425	0.16257674578734962	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	400	0.15301340779985848	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATG	340	0.1300613966298797	No Hit
ACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	333	0.12738366199338216	TruSeq Adapter, Index 15 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	306	0.11705525696689173	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTGAGTG	296	0.11322992177189527	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTAT	293	0.11208232121339631	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	275	0.1051967178624027	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT	263	0.10060631562840694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31291241895071054	0.0
2	0.0	0.0	0.0	2.1551938488610065	0.0
3	0.0	0.0	0.0	2.704894516381998	0.0
4	0.0	0.0	0.0	3.6891532620545875	0.0
5	0.0	0.0	0.0	7.314040892833234	0.0
6	0.0	0.0	0.0	8.385517280951744	0.0
7	0.0	0.0	0.0	9.812367308685424	0.0
8	0.0	0.0	0.0	11.543331484421323	0.0
9	0.0	0.0	0.0	12.145056710594266	0.0
10	0.0	0.0	0.0	16.049958877646652	0.0
11	0.0	0.0	0.0	17.618346307595203	0.0
12	0.0	0.0	0.0	21.773807929919858	0.0
13	0.0	0.0	0.0	22.514010290151674	0.0
14	0.0	0.0	0.0	22.848344586194365	0.0
15	0.0	0.0	0.0	23.661228315131112	0.0
16	0.0	0.0	0.0	24.559034485396783	0.0
17	0.0	0.0	0.0	25.61329686513781	0.0
18	0.0	0.0	0.0	26.837786661056175	0.0
19	0.0	0.0	0.0	28.06457165809154	0.0
20	0.0	0.0	0.0	28.80936442055735	0.0
21	0.0	0.0	0.0	29.53809077520418	0.0
22	0.0	0.0	0.0	30.359772775089418	0.0
23	0.0	0.0	0.0	31.09155939789224	0.0
24	0.0	0.0	0.0	31.66765487825871	0.0
25	0.0	0.0	0.0	32.14276150947727	0.0
26	0.0	0.0	0.0	32.618250674215325	0.0
27	3.8253351949964616E-4	0.0	0.0	33.16527360709982	0.0
28	3.8253351949964616E-4	0.0	0.0	33.636172369603884	0.0
29	7.650670389992923E-4	0.0	0.0	34.104775930990954	0.0
30	7.650670389992923E-4	0.0	0.0	34.73748637224337	0.0
31	7.650670389992923E-4	0.0	0.0	35.27073809842587	0.0
32	7.650670389992923E-4	0.0	0.0	35.72595298663045	0.0
33	7.650670389992923E-4	0.0	0.0	36.217891092707	0.0
34	7.650670389992923E-4	0.0	0.0	36.65627450605359	0.0
35	0.0011476005584989384	0.0	0.0	37.17498995849511	0.0
36	0.0015301340779985846	0.0	0.0	37.650479123233175	0.0
37	0.0015301340779985846	0.0	0.0	38.10225120976226	0.0
38	0.0015301340779985846	0.0	0.0	38.580800642656314	0.0
39	0.0015301340779985846	0.0	0.0	39.044431268289884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGC	20	7.023277E-4	45.000004	6
CGGGTGA	30	2.1590458E-6	45.000004	6
GAGTGCG	20	7.023277E-4	45.000004	1
GTCGAAT	30	2.1590458E-6	45.000004	43
GGTAACG	20	7.023277E-4	45.000004	1
ACGCACG	20	7.023277E-4	45.000004	1
TGGACCA	20	7.023277E-4	45.000004	22
GGCGTAG	20	7.023277E-4	45.000004	1
GGCAACG	20	7.023277E-4	45.000004	1
ATAGCGG	35	1.2071177E-7	45.000004	2
CGCGAGG	20	7.023277E-4	45.000004	2
ATCACGC	20	7.023277E-4	45.000004	11
AGAACGA	20	7.023277E-4	45.000004	32
CCGTCGA	20	7.023277E-4	45.000004	41
ACGGCTA	20	7.023277E-4	45.000004	30
CTAGTAG	20	7.023277E-4	45.000004	1
GTGTCCA	35	1.2071177E-7	45.000004	9
TCTCGCG	20	7.023277E-4	45.000004	32
AGTCTTC	20	7.023277E-4	45.000004	11
GTCGTAA	20	7.023277E-4	45.000004	15
>>END_MODULE