##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935645.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	289194
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.913269293277178	33.0	31.0	34.0	30.0	34.0
2	31.9589479726412	33.0	31.0	34.0	30.0	34.0
3	31.9949826068314	33.0	31.0	34.0	30.0	34.0
4	35.70859699717145	37.0	35.0	37.0	35.0	37.0
5	35.68616568808481	37.0	35.0	37.0	35.0	37.0
6	35.59036840321722	37.0	35.0	37.0	33.0	37.0
7	35.77482935330608	37.0	35.0	37.0	35.0	37.0
8	35.43107049247218	37.0	35.0	37.0	33.0	37.0
9	37.14613027932806	39.0	37.0	39.0	34.0	39.0
10	37.02361390623595	39.0	37.0	39.0	33.0	39.0
11	37.04292965967482	39.0	37.0	39.0	33.0	39.0
12	36.829833952295	39.0	35.0	39.0	33.0	39.0
13	36.53670546415209	39.0	35.0	39.0	32.0	39.0
14	37.67611361231561	40.0	36.0	41.0	32.0	41.0
15	37.58670304363161	40.0	36.0	41.0	32.0	41.0
16	37.731215032123764	40.0	36.0	41.0	33.0	41.0
17	37.46454974861166	39.0	35.0	41.0	32.0	41.0
18	37.52560564880323	39.0	36.0	41.0	33.0	41.0
19	37.389606976631605	39.0	36.0	41.0	32.0	41.0
20	37.034658395402396	39.0	35.0	41.0	31.0	41.0
21	37.44396840874984	39.0	35.0	41.0	32.0	41.0
22	37.38575834906672	39.0	35.0	41.0	32.0	41.0
23	37.26986037054711	39.0	35.0	41.0	32.0	41.0
24	37.2693900979965	39.0	35.0	41.0	32.0	41.0
25	37.3430776572128	39.0	35.0	41.0	33.0	41.0
26	37.14458806199299	39.0	35.0	41.0	32.0	41.0
27	36.996334640414396	39.0	35.0	41.0	31.0	41.0
28	36.818744510605335	39.0	35.0	41.0	31.0	41.0
29	36.832257930662465	39.0	35.0	41.0	31.0	41.0
30	36.74422014287987	39.0	35.0	41.0	31.0	41.0
31	36.807793384371735	39.0	35.0	41.0	31.0	41.0
32	36.44356037815446	39.0	35.0	41.0	30.0	41.0
33	36.34401474442762	39.0	35.0	41.0	30.0	41.0
34	36.159837341023675	39.0	35.0	41.0	30.0	41.0
35	36.12122312357794	39.0	35.0	41.0	30.0	41.0
36	36.06212438708963	39.0	35.0	41.0	29.0	41.0
37	35.94858468709586	39.0	35.0	41.0	28.0	41.0
38	35.74788204457907	39.0	35.0	41.0	27.0	41.0
39	35.768608615669756	39.0	35.0	41.0	28.0	41.0
40	35.602716515557034	39.0	35.0	41.0	26.0	41.0
41	35.47499948131704	38.0	35.0	41.0	26.0	41.0
42	35.704665380332926	39.0	35.0	41.0	27.0	41.0
43	35.53744199395562	38.0	35.0	41.0	26.0	41.0
44	35.56823447236111	39.0	35.0	40.0	26.0	41.0
45	35.6932128605711	38.0	35.0	40.0	27.0	41.0
46	35.53919168447478	38.0	35.0	40.0	27.0	41.0
47	35.273857687227256	38.0	35.0	40.0	27.0	41.0
48	35.30571865253083	38.0	35.0	40.0	26.0	41.0
49	35.31711238822382	38.0	35.0	40.0	26.0	41.0
50	35.16789767422561	38.0	35.0	40.0	26.0	41.0
51	34.00398694302095	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	7.0
11	6.0
12	2.0
13	3.0
14	3.0
15	10.0
16	20.0
17	53.0
18	128.0
19	335.0
20	690.0
21	1548.0
22	2367.0
23	2859.0
24	3041.0
25	3230.0
26	3284.0
27	3298.0
28	3318.0
29	3656.0
30	4419.0
31	5954.0
32	8312.0
33	11461.0
34	19930.0
35	28034.0
36	20503.0
37	28317.0
38	48357.0
39	85989.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.92671355560627	15.004806462098108	20.409482907667517	13.658997074628104
2	24.39538856269494	39.71140480092948	21.366625863607126	14.526580772768453
3	24.414406937903276	38.25840093501248	23.89192030263422	13.435271824450023
4	24.956949314301127	20.250420133197782	38.57894700443301	16.21368354806808
5	35.0515570862466	25.191739800964058	20.943034779421428	18.81366833336791
6	20.769448882065326	44.14199464719185	22.916450548766573	12.17210592197625
7	64.67526988803364	7.1989736993160305	23.582439469698542	4.54331694295179
8	62.64307004986273	20.938885315739608	10.87401536684717	5.544029267550502
9	59.06242868109297	6.164374087982462	11.251616561892709	23.52158066903186
10	44.413784518351	20.302980006500825	20.281886899451578	15.001348575696591
11	37.09897162458419	21.858337309902694	22.9327026148537	18.10998845065942
12	26.752975511248504	17.439158488765326	29.628208054109006	26.17965794587716
13	27.145099829180413	22.261872652959607	34.394904458598724	16.198123059261256
14	19.39044378514077	33.35753853814394	24.87361425202459	22.378403424690692
15	14.729904493177589	22.217957495660354	40.26086295013036	22.791275061031698
16	16.04701342351501	23.4213019633879	28.169671569949585	32.362013043147506
17	16.908372926132632	25.686217556380836	32.58608408196574	24.819325435520792
18	19.28186615213317	22.528475694516484	29.133038721411925	29.056619431938422
19	21.597266886588244	25.07486324059282	24.982537673672343	28.345332199146593
20	25.290981140687567	23.55546795576672	33.40422000456441	17.749330898981306
21	22.912301085084753	32.83505190287489	26.006072048521062	18.2465749635193
22	19.368659100811218	20.956174747747188	33.10718756267419	26.5679785887674
23	21.454801966845785	30.340532652821288	27.337358313104698	20.86730706722823
24	21.949279722262563	24.734607218683653	25.19658084192618	28.119532217127603
25	18.47272073417844	34.21820646348126	26.336645988505985	20.97242681383431
26	18.806406771924728	22.201705429573227	31.893123647101945	27.0987641514001
27	26.486372469691627	26.33076758162341	23.39536781537653	23.787492133308437
28	17.2821704461365	26.666874139850762	34.93018527355339	21.120770140459346
29	23.49115126869852	19.584085423625663	32.90524699682566	24.019516310850157
30	22.58380187694074	30.069088570302288	27.785154602101013	19.56195495065596
31	26.26403037407415	23.794407906111466	22.95033783550143	26.991223884312955
32	27.240883282502402	27.794836684025253	21.74146074953146	23.222819283940886
33	24.62153433335408	26.19383528012338	20.57476987765998	28.60986050886256
34	22.115258269535328	23.54094483288035	30.50996908649557	23.83382781108875
35	23.494263366459887	22.814788688562004	24.2249147631002	29.46603318187791
36	24.004301610683484	28.42901305006328	26.83700215080534	20.729683188447893
37	24.076225647835017	22.070651534955772	29.627170688188553	24.22595212902066
38	23.500833350622766	28.07285075070714	22.376674481489932	26.049641417180165
39	21.6204347254784	24.082104054717597	27.49987897397595	26.79758224582806
40	19.8738563040727	25.22873918546028	28.391322088286756	26.506082422180267
41	17.946430423867714	27.464608532680483	28.301071253207187	26.287889790244613
42	19.58996383050824	22.44998167320207	28.764773819650475	29.195280676639214
43	22.370450285967205	26.761274438612144	26.28062822880143	24.587647046619225
44	24.54165715747906	22.283657337289156	29.32944666901803	23.845238836213753
45	21.428176241554112	22.736640455887745	25.710768549831602	30.12441475272654
46	27.686604839657807	26.550343368119673	23.255323416115132	22.507728376107387
47	17.45056951389033	24.994986064717803	36.60103598276589	20.953408438625974
48	22.391889181656605	24.677552093058637	25.582826752975514	27.347731972309248
49	20.569928836697855	19.51146980919383	34.633844408943474	25.284756945164837
50	22.287806800970973	22.555101419808157	29.28345677987787	25.873634999343004
51	19.74003610033403	22.693416875868795	26.47703617640753	31.08951084738964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	517.0
1	581.5
2	646.0
3	1058.5
4	1471.0
5	1068.5
6	666.0
7	683.0
8	700.0
9	690.0
10	680.0
11	687.0
12	694.0
13	712.0
14	730.0
15	713.5
16	697.0
17	694.5
18	692.0
19	623.0
20	554.0
21	661.5
22	769.0
23	863.0
24	957.0
25	1153.0
26	1426.5
27	1504.0
28	1807.0
29	2110.0
30	2426.0
31	2742.0
32	3110.5
33	3479.0
34	4524.5
35	5570.0
36	5975.5
37	6381.0
38	6912.5
39	7444.0
40	8863.0
41	10282.0
42	12321.5
43	14361.0
44	16280.0
45	18199.0
46	36846.5
47	55494.0
48	43032.5
49	30571.0
50	28352.0
51	26133.0
52	22160.0
53	18187.0
54	16709.0
55	15231.0
56	14361.0
57	13491.0
58	12531.0
59	11571.0
60	10949.0
61	10327.0
62	9793.5
63	9260.0
64	7579.5
65	5899.0
66	4621.5
67	3344.0
68	2872.5
69	2401.0
70	2175.0
71	1949.0
72	1502.0
73	1055.0
74	743.0
75	372.0
76	313.0
77	268.5
78	224.0
79	129.5
80	35.0
81	48.5
82	62.0
83	35.0
84	8.0
85	9.5
86	11.0
87	6.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	289194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.565903856926493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07446600804406	20.475528537936473
2	8.593333073008187	4.565793204561644
3	2.77246280604475	2.2095894105686837
4	1.3693102685253882	1.4550785977579064
5	0.9723144207114686	1.2915205709662025
6	0.7171957775261301	1.143177244341169
7	0.5883348302029234	1.0940752574396426
8	0.5024275319874524	1.0677953207881214
9	0.4256316138857433	1.0176559679661403
>10	6.463873377848933	40.01950247930455
>50	0.45687063141864187	7.446212577024419
>100	0.044255274838273004	2.3216249299778005
>500	0.0065081286526872065	1.0626084911858475
>1k	0.009111380113762089	5.013935282198109
>5k	0.0026032514610748823	6.084842700747595
>10k+	0.0013016257305374411	3.7310594272356967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGC	10790	3.7310594272356967	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCG	9552	3.302973090728024	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTC	8045	2.7818696100195717	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3884	1.34304307834879	No Hit
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	2216	0.7662676265759317	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGGCATCAT	2040	0.7054088259092512	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCT	1829	0.632447422837265	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGCATCATCGTA	1640	0.5670933698486137	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCT	1449	0.5010477395796593	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTC	1442	0.49862721909859814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	712	0.24620151178793476	No Hit
CCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	699	0.24170625946596402	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTT	570	0.19709952488640844	No Hit
GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT	564	0.19502479304549886	No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT	528	0.1825764020000415	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATG	491	0.16978222231443252	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGGCATCATCGTAT	434	0.1500722698257917	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGGCATCA	403	0.13935282198109228	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGGCATCATCGT	365	0.12621285365533172	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010373659204547811	0.0	0.0	0.18983796344322496	0.0
2	0.0010373659204547811	0.0	0.0	1.3748556332427366	0.0
3	0.0010373659204547811	0.0	0.0	1.6303934383147645	0.0
4	0.0010373659204547811	0.0	0.0	2.4094552445763053	0.0
5	0.0010373659204547811	0.0	0.0	5.95793826981196	0.0
6	0.0010373659204547811	0.0	0.0	6.62185245890302	0.0
7	0.0010373659204547811	0.0	0.0	7.693451454732809	0.0
8	0.001383154560606375	0.0	0.0	8.867058099407318	0.0
9	0.001383154560606375	0.0	0.0	9.142305856967987	0.0
10	0.001383154560606375	0.0	0.0	13.021362822188566	0.0
11	0.001383154560606375	0.0	0.0	13.783480985082678	0.0
12	0.001383154560606375	0.0	0.0	18.337517375879166	0.0
13	0.001383154560606375	0.0	0.0	18.901498647966417	0.0
14	0.001383154560606375	0.0	0.0	19.2445209789968	0.0
15	0.001383154560606375	0.0	0.0	20.052974819671224	0.0
16	0.001383154560606375	0.0	0.0	20.6010498143115	0.0
17	0.001383154560606375	0.0	0.0	21.117658042697983	0.0
18	0.001383154560606375	0.0	0.0	21.755983872417822	0.0
19	0.001383154560606375	0.0	0.0	22.893282709876416	0.0
20	0.001383154560606375	0.0	0.0	23.386377310732588	0.0
21	0.001383154560606375	0.0	0.0	23.748072228331154	0.0
22	0.001383154560606375	0.0	0.0	24.26986728631991	0.0
23	0.001383154560606375	0.0	0.0	24.628450106157114	0.0
24	0.001383154560606375	0.0	0.0	24.93412726405112	0.0
25	0.001383154560606375	0.0	0.0	25.230468128661037	0.0
26	0.001383154560606375	0.0	0.0	25.484622779172458	0.0
27	0.001383154560606375	0.0	0.0	25.905447554236947	0.0
28	0.0017289432007579688	0.0	0.0	26.195910011964287	0.0
29	0.0017289432007579688	0.0	0.0	26.46389620808177	0.0
30	0.0017289432007579688	0.0	0.0	26.824899548400037	0.0
31	0.0017289432007579688	0.0	0.0	27.08424102851373	0.0
32	0.0017289432007579688	0.0	0.0	27.33390042670318	0.0
33	0.0017289432007579688	0.0	0.0	27.60914818426385	0.0
34	0.0017289432007579688	0.0	0.0	27.90030221927149	0.0
35	0.0017289432007579688	0.0	0.0	28.200446758923075	0.0
36	0.0017289432007579688	0.0	0.0	28.489180273449655	0.0
37	0.0017289432007579688	0.0	0.0	28.75025069676411	0.0
38	0.0020747318409095623	0.0	0.0	29.031376861207356	0.0
39	0.0020747318409095623	0.0	0.0	29.33152140085894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	30	2.159848E-6	45.000004	16
CATGCGG	30	2.159848E-6	45.000004	2
GCGAACC	70	0.0	45.000004	33
TCGGGCA	35	1.207718E-7	45.000004	5
GTAGGGT	35	1.207718E-7	45.000004	4
TATTGCG	30	2.159848E-6	45.000004	1
TTATACG	30	2.159848E-6	45.000004	26
CTTTGAT	35	1.207718E-7	45.000004	41
TTCGGAC	35	1.207718E-7	45.000004	13
ATACGGT	30	2.159848E-6	45.000004	28
TGGTCTT	30	2.159848E-6	45.000004	16
CAAACGA	25	3.883541E-5	45.0	15
ATTAGCG	25	3.883541E-5	45.0	1
TGCCACG	20	7.0245203E-4	45.0	40
GCGAATT	20	7.0245203E-4	45.0	17
GCGAATC	20	7.0245203E-4	45.0	38
TTAGCGG	55	1.8189894E-12	45.0	2
TCTTCGT	20	7.0245203E-4	45.0	23
TATGGGC	40	6.7848305E-9	45.0	4
GCACGGT	25	3.883541E-5	45.0	3
>>END_MODULE