FastQCFastQC Report
Sat 14 Jan 2017
SRR2935643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956919
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC180991.891382656212281No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179371.874453323635543No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG95010.9928740050098285No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC82740.8646499860489759No Hit
GAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT60160.6286843505040657Illumina Single End Adapter 1 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT35180.3676382222528762No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC34260.3580240333821358Illumina Single End Adapter 1 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT31740.33168951604054264No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC28030.29291925439875266No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT22760.23784667249788122No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT19330.2020024683384905No Hit
CGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG19100.1995989211208054Illumina Single End Adapter 1 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC17080.17848950642635375No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGAGAGGT14870.15539455272598832No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC13780.14400382895521982No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT10820.11307122128414213Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA10310.10774161658405779No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC10300.10763711453111496Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC10020.10471105704871572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAA207.0326944E-445.045
TCGAGCG207.0326944E-445.01
CGTCATA406.8121153E-945.038
CGTTTTT103500.043.9130441
CATACGG501.0804797E-940.52
GCCGATT403.4574077E-739.3759
CCGATGA7700.038.86363618
CGATGAA7850.038.69426719
CGTTTCT4250.038.647061
GTTAGCG659.094947E-1238.076921
TACGGCT10550.037.9620867
GTTTTTT122500.037.5061232
GTCGAAT301.139779E-437.50000443
AACGGGC601.546141E-1037.5000044
TCGGACG301.139779E-437.5000041
AATTGCG301.139779E-437.5000041
CTACGCG301.139779E-437.5000041
CGATCCC301.139779E-437.50000434
AGGGCGA6050.037.1900836
TAGAACG552.748493E-936.818181
Produced by FastQC (version 0.11.5)