FastQCFastQC Report
Sat 14 Jan 2017
SRR2935641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866395
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC162191.8720098800200833No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC127921.4764628143052534No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG125031.4431062044448548No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69740.8049446268734238No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT45290.5227407822067303RNA PCR Primer, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC41620.480381350307885RNA PCR Primer, Index 14 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT26450.3052880037396338No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC22710.2621206262732356No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATAT22120.2553107993467183No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT21200.24469208617316582No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTA18070.20856537722401444No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC17020.19644619371072086Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT12360.14266010307077026Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC11530.13308017705550007No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT11390.13146428592039427No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG11220.12950213239919436Illumina PCR Primer Index 7 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC10250.11830631524881838RNA PCR Primer, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.890007E-545.0000041
CTATACG253.890007E-545.0000041
TAGCGGA302.1648793E-645.0000043
ACGATAG253.890007E-545.0000041
CTACGCT207.032323E-445.00000427
CGTTTTT40750.043.3987731
GTTAGCG556.002665E-1140.9090921
GCGTAAG1000.040.5000041
TACGGCT16500.039.9545447
GATCGAG403.4568984E-739.3750041
GCGGGTC800.039.3750045
CGCGGGT1050.038.571434
AAGTACG356.2465097E-638.5714261
TTTAACG356.2465097E-638.5714261
CGTTCGG356.2465097E-638.5714262
GCCTCGT356.2465097E-638.57142641
CCGACAG356.2465097E-638.5714261
GCGTACG356.2465097E-638.5714261
GTACGAG356.2465097E-638.5714261
ACGGCTG17000.038.3823558
Produced by FastQC (version 0.11.5)