##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935641.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866395
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.020202101812686	33.0	31.0	34.0	30.0	34.0
2	32.05755111698475	33.0	31.0	34.0	30.0	34.0
3	32.04214128659561	33.0	31.0	34.0	30.0	34.0
4	35.73485419468025	37.0	35.0	37.0	35.0	37.0
5	35.788526018732796	37.0	35.0	37.0	35.0	37.0
6	35.710278798931206	37.0	35.0	37.0	35.0	37.0
7	35.997937430386834	37.0	35.0	37.0	35.0	37.0
8	35.77348668909678	37.0	35.0	37.0	35.0	37.0
9	37.65049198114024	39.0	38.0	39.0	35.0	39.0
10	37.24336705544238	39.0	37.0	39.0	34.0	39.0
11	37.2598306776932	39.0	37.0	39.0	34.0	39.0
12	37.292660968726736	39.0	37.0	39.0	34.0	39.0
13	37.26710334200913	39.0	37.0	39.0	34.0	39.0
14	38.48463691503298	40.0	38.0	41.0	34.0	41.0
15	38.46575522711927	40.0	38.0	41.0	34.0	41.0
16	38.3503286607148	40.0	38.0	41.0	34.0	41.0
17	38.31699282659757	40.0	38.0	41.0	34.0	41.0
18	38.202506939675324	40.0	38.0	41.0	34.0	41.0
19	38.0409686113147	40.0	37.0	41.0	34.0	41.0
20	38.03200964917849	40.0	37.0	41.0	34.0	41.0
21	38.12820134003543	40.0	37.0	41.0	34.0	41.0
22	38.22389787568026	40.0	37.0	41.0	34.0	41.0
23	38.21106192902775	40.0	37.0	41.0	34.0	41.0
24	38.13012886731803	40.0	37.0	41.0	34.0	41.0
25	37.95192146769083	40.0	36.0	41.0	34.0	41.0
26	38.02594774900594	40.0	37.0	41.0	34.0	41.0
27	38.017936391599676	40.0	37.0	41.0	34.0	41.0
28	37.90057075583308	40.0	37.0	41.0	33.0	41.0
29	37.867067561562564	40.0	37.0	41.0	33.0	41.0
30	37.71811933356033	40.0	36.0	41.0	33.0	41.0
31	37.66034891706439	40.0	36.0	41.0	33.0	41.0
32	37.49903912187859	40.0	37.0	41.0	33.0	41.0
33	37.33073251807778	40.0	36.0	41.0	32.0	41.0
34	37.1689044835208	40.0	36.0	41.0	32.0	41.0
35	37.045160694602345	40.0	36.0	41.0	31.0	41.0
36	36.961067411515536	40.0	36.0	41.0	31.0	41.0
37	36.94951032727567	40.0	36.0	41.0	31.0	41.0
38	36.82077458895769	40.0	36.0	41.0	31.0	41.0
39	36.72595525135764	40.0	36.0	41.0	30.0	41.0
40	36.6650073003653	40.0	36.0	41.0	30.0	41.0
41	36.422661718961905	40.0	35.0	41.0	29.0	41.0
42	36.5284910462318	40.0	35.0	41.0	30.0	41.0
43	36.508481697147374	40.0	35.0	41.0	30.0	41.0
44	36.445573901049755	40.0	35.0	41.0	30.0	41.0
45	36.4268226386348	39.0	35.0	41.0	30.0	41.0
46	36.29883136444693	39.0	35.0	41.0	29.0	41.0
47	36.14328568378165	39.0	35.0	41.0	28.0	41.0
48	36.07988965771963	39.0	35.0	41.0	28.0	41.0
49	36.07950876909493	39.0	35.0	41.0	28.0	41.0
50	35.960716532297624	39.0	35.0	41.0	28.0	41.0
51	34.796457735790256	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	13.0
10	9.0
11	10.0
12	9.0
13	15.0
14	15.0
15	16.0
16	36.0
17	92.0
18	203.0
19	469.0
20	945.0
21	1801.0
22	2763.0
23	3854.0
24	5226.0
25	7473.0
26	9952.0
27	11022.0
28	10747.0
29	10909.0
30	12375.0
31	15424.0
32	20026.0
33	26337.0
34	41264.0
35	55152.0
36	57104.0
37	84314.0
38	164873.0
39	323765.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99967105073321	17.40199331713595	21.647631853831104	17.950703778299737
2	29.362934919984536	30.674576838508994	24.189197767761815	15.773290473744655
3	27.937488097230478	29.599432129686804	28.168098846369148	14.294980926713565
4	24.50452738069818	23.14752508959539	35.33226761465613	17.015679915050296
5	28.251548081417827	26.676400487075757	27.106573791399995	17.965477640106418
6	22.862320304249216	35.90510102205114	27.08602888982508	14.14654978387456
7	75.29602548491162	4.25948903213892	16.51325319282775	3.931232290121711
8	76.10777993871156	8.63382175566572	11.103826776470317	4.154571529152407
9	70.38694821646015	6.763081504394647	12.01749779257729	10.832472486567905
10	31.812972143191036	36.498594751816434	19.45833020735346	12.230102897639068
11	24.458474483347665	24.195199649120784	33.60799635270287	17.73832951482869
12	21.400169668569184	22.036599934210148	35.38074434870931	21.182486048511358
13	21.550332123338663	23.700852382573768	38.35883171070932	16.389983783378252
14	19.20244230402992	29.564805891077395	31.840673134078568	19.39207867081412
15	16.819464562930303	26.782241356425185	38.07385776695387	18.324436313690637
16	20.1860583221279	28.235620011657502	29.869978474021664	21.708343192192938
17	20.3992405311665	27.231574512779964	32.22860242729932	20.140582528754205
18	20.26396735899907	26.717374869430227	31.766457562659063	21.25220020891164
19	19.422203498404308	28.144899266500843	30.32692940287052	22.105967832224334
20	21.78844522417604	27.669481010393643	33.12507574489696	17.41699802053336
21	21.983044685160927	29.391674698030346	31.69270367442102	16.93257694238771
22	20.555289446499575	23.761448300140238	33.0657494560795	22.617512797280686
23	19.409160948528097	28.560875812995228	32.608913948026014	19.42104929045066
24	20.448525210787228	26.766890390641684	30.753640083333817	22.030944315237278
25	19.026425591098747	32.470870676769835	28.95850045302662	19.544203279104796
26	20.42948077955205	26.60622464349402	30.676308150439464	22.287986426514465
27	21.680065097328587	27.29367090068618	29.092619417240407	21.933644584744833
28	18.072703559000225	27.06248304757068	33.10753178400152	21.75728160942757
29	22.186069864207433	24.51583861864392	30.485979258883074	22.81211225826557
30	19.99342101466421	26.66959066014924	30.935889519214676	22.401098805971873
31	21.269744169807076	25.43181805065819	29.716584236981973	23.58185354255276
32	21.754280668748088	26.912089751210477	28.71738641150976	22.616243168531675
33	19.60883892450903	25.81524593285972	27.93771893882121	26.63819620381004
34	21.712960024007526	27.591802815113198	29.51852215213615	21.176715008743123
35	19.225411042307492	26.089601163441618	29.854281245852064	24.830706548398826
36	21.086571367563295	28.83453851880493	29.520368884861988	20.558521228769788
37	18.729794147011468	27.188868818495028	30.327391086051975	23.75394594844153
38	18.679124417846364	27.13923787648821	29.756058148996704	24.425579556668726
39	20.906630347589726	28.1751395148864	27.982617628218076	22.935612509305802
40	20.491461746662896	25.701094766244033	31.39122455692842	22.416218930164646
41	19.318671044962173	30.579700944719214	28.252933130962205	21.848694879356415
42	20.44298501260972	25.876649795993746	32.35394941106539	21.326415780331143
43	22.095464539846144	27.08776020175555	27.83487901015126	22.981896248247047
44	21.244236174031474	25.09282717467206	29.49959314169634	24.163343509600125
45	19.90916383404798	24.329895717311388	29.140057364135295	26.620883084505337
46	23.79007265739068	26.650546228914063	27.722689997056772	21.836691116638484
47	18.81335880285551	25.23075502513288	33.76000554019818	22.195880631813434
48	20.47876545917278	26.29816654066563	29.437150491404037	23.785917508757553
49	20.330334316333772	23.827007311907387	32.92239682823654	22.920261543522297
50	20.80194368619394	24.125600909515867	31.14976425302547	23.922691151264726
51	20.38423582776909	23.853669515636632	28.955153249961047	26.806941406633232
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	359.0
1	627.0
2	895.0
3	5984.0
4	11073.0
5	7397.0
6	3721.0
7	3812.5
8	3904.0
9	3863.5
10	3823.0
11	3856.5
12	3890.0
13	3845.0
14	3800.0
15	3645.5
16	3491.0
17	3426.0
18	3361.0
19	3423.0
20	3485.0
21	3577.0
22	3669.0
23	4327.0
24	4985.0
25	5457.5
26	6900.0
27	7870.0
28	10427.5
29	12985.0
30	14769.0
31	16553.0
32	18826.0
33	21099.0
34	24191.0
35	27283.0
36	29999.0
37	32715.0
38	36233.0
39	39751.0
40	42661.0
41	45571.0
42	48959.0
43	52347.0
44	56050.5
45	59754.0
46	93232.0
47	126710.0
48	104314.5
49	81919.0
50	79532.0
51	77145.0
52	66473.0
53	55801.0
54	49084.5
55	42368.0
56	37931.0
57	33494.0
58	29397.5
59	25301.0
60	22037.0
61	18773.0
62	15655.0
63	12537.0
64	10332.0
65	8127.0
66	6470.0
67	4813.0
68	3848.0
69	2883.0
70	2142.0
71	1401.0
72	1194.5
73	988.0
74	940.5
75	698.5
76	504.0
77	349.0
78	194.0
79	173.5
80	153.0
81	98.5
82	44.0
83	28.5
84	13.0
85	10.0
86	7.0
87	6.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	866395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.82400981790722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32664149095592	23.335477587223444
2	8.963385727896231	5.705017508125183
3	3.430101169543459	3.2747871988789825
4	2.074696395895674	2.6410063388864256
5	1.4986589168086308	2.384666804110603
6	1.1639580238784766	2.222508694772432
7	0.9650895647436393	2.1499113848492972
8	0.8117656292632036	2.066690988440946
9	0.7134270899329298	2.0433699642947882
>10	6.921504101616104	41.750048823070024
>50	0.10126625757293514	2.0443267807863905
>100	0.021856023624276712	1.3254794188511045
>500	0.001457068241618429	0.291549828442409
>1k	0.004735471785259894	3.144217096160421
>5k	3.6426706040460725E-4	0.8084566614542189
>10k+	0.0010928011812138217	4.812484921653347
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	16219	1.8720098800200833	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC	12792	1.4764628143052534	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG	12503	1.4431062044448548	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6974	0.8049446268734238	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	4529	0.5227407822067303	RNA PCR Primer, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	4162	0.480381350307885	RNA PCR Primer, Index 14 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	2645	0.3052880037396338	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC	2271	0.2621206262732356	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATAT	2212	0.2553107993467183	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	2120	0.24469208617316582	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTA	1807	0.20856537722401444	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1702	0.19644619371072086	Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	1236	0.14266010307077026	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	1153	0.13308017705550007	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	1139	0.13146428592039427	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1122	0.12950213239919436	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	1025	0.11830631524881838	RNA PCR Primer, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1542079536470086E-4	0.0	0.0	0.1503932963602052	0.0
2	1.1542079536470086E-4	0.0	0.0	0.9698809434495813	0.0
3	1.1542079536470086E-4	0.0	0.0	1.334264394415942	0.0
4	1.1542079536470086E-4	0.0	0.0	2.2218503107704914	0.0
5	1.1542079536470086E-4	0.0	0.0	4.909769793223645	0.0
6	1.1542079536470086E-4	0.0	0.0	5.940477495830423	0.0
7	1.1542079536470086E-4	0.0	0.0	6.90851170655417	0.0
8	1.1542079536470086E-4	0.0	0.0	8.080263621096613	0.0
9	1.1542079536470086E-4	0.0	0.0	8.530058460632851	0.0
10	1.1542079536470086E-4	0.0	0.0	10.722591889380709	0.0
11	1.1542079536470086E-4	0.0	0.0	11.5900945873418	0.0
12	1.1542079536470086E-4	0.0	0.0	13.892277771686125	0.0
13	1.1542079536470086E-4	0.0	0.0	14.33387773475147	0.0
14	1.1542079536470086E-4	0.0	0.0	14.551215092423202	0.0
15	1.1542079536470086E-4	0.0	0.0	14.976540723342124	0.0
16	1.1542079536470086E-4	0.0	0.0	15.42402714697107	0.0
17	1.1542079536470086E-4	0.0	0.0	15.991320356188574	0.0
18	1.1542079536470086E-4	0.0	0.0	16.607782824231442	0.0
19	1.1542079536470086E-4	0.0	0.0	17.264642570651954	0.0
20	1.1542079536470086E-4	0.0	0.0	17.695623820543748	0.0
21	1.1542079536470086E-4	0.0	0.0	18.114947570103705	0.0
22	1.1542079536470086E-4	0.0	0.0	18.676931422734434	0.0
23	4.6168318145880345E-4	0.0	0.0	19.140576757714438	0.0
24	4.6168318145880345E-4	0.0	0.0	19.50703778299736	0.0
25	5.771039768235043E-4	0.0	0.0	19.83448657944702	0.0
26	6.925247721882051E-4	0.0	0.0	20.17163072270731	0.0
27	6.925247721882051E-4	0.0	0.0	20.5480179363916	0.0
28	6.925247721882051E-4	0.0	0.0	20.896473317597632	0.0
29	9.233663629176069E-4	0.0	0.0	21.267320333104415	0.0
30	9.233663629176069E-4	0.0	0.0	21.733274083991713	0.0
31	0.0012696287490117095	0.0	0.0	22.096041643822968	0.0
32	0.0012696287490117095	0.0	0.0	22.465272768194644	0.0
33	0.0012696287490117095	0.0	0.0	22.827694065639808	0.0
34	0.0012696287490117095	0.0	0.0	23.20904437352478	0.0
35	0.0012696287490117095	0.0	0.0	23.590510102205116	0.0
36	0.0012696287490117095	0.0	0.0	23.962742167256273	0.0
37	0.0012696287490117095	0.0	0.0	24.315006434709343	0.0
38	0.0012696287490117095	0.0	0.0	24.705821247814217	0.0
39	0.0012696287490117095	0.0	0.0	25.12295200226225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.890007E-5	45.000004	1
CTATACG	25	3.890007E-5	45.000004	1
TAGCGGA	30	2.1648793E-6	45.000004	3
ACGATAG	25	3.890007E-5	45.000004	1
CTACGCT	20	7.032323E-4	45.000004	27
CGTTTTT	4075	0.0	43.398773	1
GTTAGCG	55	6.002665E-11	40.909092	1
GCGTAAG	100	0.0	40.500004	1
TACGGCT	1650	0.0	39.954544	7
GATCGAG	40	3.4568984E-7	39.375004	1
GCGGGTC	80	0.0	39.375004	5
CGCGGGT	105	0.0	38.57143	4
AAGTACG	35	6.2465097E-6	38.571426	1
TTTAACG	35	6.2465097E-6	38.571426	1
CGTTCGG	35	6.2465097E-6	38.571426	2
GCCTCGT	35	6.2465097E-6	38.571426	41
CCGACAG	35	6.2465097E-6	38.571426	1
GCGTACG	35	6.2465097E-6	38.571426	1
GTACGAG	35	6.2465097E-6	38.571426	1
ACGGCTG	1700	0.0	38.382355	8
>>END_MODULE