##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935640.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246489
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970221794887397	33.0	31.0	34.0	30.0	34.0
2	31.991180133799073	33.0	31.0	34.0	30.0	34.0
3	32.0140817642978	33.0	31.0	34.0	30.0	34.0
4	35.699025108625534	37.0	35.0	37.0	33.0	37.0
5	35.76763263269355	37.0	35.0	37.0	35.0	37.0
6	35.714883828487274	37.0	35.0	37.0	35.0	37.0
7	36.0003813557603	37.0	35.0	37.0	35.0	37.0
8	35.75299506266	37.0	35.0	37.0	35.0	37.0
9	37.572759839181465	39.0	37.0	39.0	35.0	39.0
10	37.20964018678318	39.0	37.0	39.0	34.0	39.0
11	37.226034427499805	39.0	37.0	39.0	34.0	39.0
12	37.05474483648358	39.0	37.0	39.0	34.0	39.0
13	36.86986031831035	39.0	35.0	39.0	33.0	39.0
14	37.9507604801837	40.0	37.0	41.0	33.0	41.0
15	38.022828604927604	40.0	37.0	41.0	33.0	41.0
16	37.920458925144736	40.0	36.0	41.0	33.0	41.0
17	37.93044314350742	40.0	36.0	41.0	33.0	41.0
18	37.88192982242615	40.0	36.0	41.0	33.0	41.0
19	37.772440149459	39.0	36.0	41.0	33.0	41.0
20	37.863754569169416	40.0	36.0	41.0	34.0	41.0
21	37.870192990356564	40.0	36.0	41.0	34.0	41.0
22	37.97419763153731	40.0	36.0	41.0	34.0	41.0
23	38.008685985987206	40.0	36.0	41.0	34.0	41.0
24	37.90916836045422	40.0	36.0	41.0	34.0	41.0
25	37.7415341049702	39.0	35.0	41.0	33.0	41.0
26	37.80291615447342	40.0	36.0	41.0	34.0	41.0
27	37.79049369343054	40.0	36.0	41.0	34.0	41.0
28	37.634393421207434	40.0	36.0	41.0	33.0	41.0
29	37.644933445305874	40.0	36.0	41.0	33.0	41.0
30	37.52739067463457	40.0	36.0	41.0	33.0	41.0
31	37.411766853693265	39.0	35.0	41.0	33.0	41.0
32	37.3625679036387	40.0	35.0	41.0	33.0	41.0
33	37.30052862399539	40.0	35.0	41.0	33.0	41.0
34	37.239122232635125	40.0	35.0	41.0	33.0	41.0
35	37.18051109785832	40.0	35.0	41.0	32.0	41.0
36	37.000434096450554	39.0	35.0	41.0	31.0	41.0
37	36.99417012523885	39.0	35.0	41.0	32.0	41.0
38	36.79642093561984	39.0	35.0	41.0	31.0	41.0
39	36.88293189554098	39.0	35.0	41.0	31.0	41.0
40	36.80643355281575	39.0	35.0	41.0	31.0	41.0
41	36.628989528944494	39.0	35.0	41.0	31.0	41.0
42	36.73574479997079	39.0	35.0	41.0	31.0	41.0
43	36.678046484833	39.0	35.0	41.0	31.0	41.0
44	36.6037673080746	39.0	35.0	41.0	31.0	41.0
45	36.598152453050645	39.0	35.0	41.0	31.0	41.0
46	36.49428980603597	39.0	35.0	41.0	31.0	41.0
47	36.366032561290766	39.0	35.0	41.0	31.0	41.0
48	36.30022029380621	39.0	35.0	40.0	31.0	41.0
49	36.3352887958489	39.0	35.0	40.0	31.0	41.0
50	36.16832394143349	39.0	35.0	40.0	30.0	41.0
51	35.0698286738962	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	5.0
11	3.0
12	6.0
13	6.0
14	6.0
15	10.0
16	12.0
17	22.0
18	53.0
19	103.0
20	217.0
21	407.0
22	665.0
23	825.0
24	1114.0
25	1447.0
26	1884.0
27	2145.0
28	2444.0
29	2861.0
30	3552.0
31	4731.0
32	6343.0
33	8772.0
34	16459.0
35	23654.0
36	16335.0
37	24411.0
38	47562.0
39	80385.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5703459383583	18.1375233783252	23.467578674910442	13.824552008406055
2	28.034922450900446	30.379043283878794	24.491153763453948	17.094880501766813
3	28.50796587271643	29.127060436774055	25.814133693592815	16.550839996916697
4	25.639278020520184	24.445715630312105	32.81769166169687	17.097314687470842
5	27.337933944313946	27.002016317158166	26.320038622413172	19.340011116114713
6	23.466767279675764	36.7407876213543	25.472536299794314	14.319908799175623
7	74.41508546020309	5.477323531678898	14.910604530019594	5.196986478098414
8	74.00249098337045	9.938780229543712	9.96231069134931	6.0964180957365235
9	69.1519702704786	7.084291793954294	11.729935210090511	12.033802725476592
10	38.78590931035462	24.38283249962473	20.666642324809626	16.164615865211022
11	31.813184361168247	26.2989423463116	24.557282475080026	17.33059081744013
12	28.48118982997213	20.94292240221673	29.483262944796724	21.092624823014415
13	22.745031218431656	27.3395567347833	31.438319762748034	18.477092284037017
14	18.82883211826897	31.943413296333713	28.463339134809264	20.764415450588057
15	16.260360502902767	25.240882960294375	40.60749161220176	17.891264924601096
16	18.371610903529163	25.084689377619284	32.954006061122406	23.58969365772915
17	18.572836921728758	25.531362454308304	30.052051004304452	25.843749619658485
18	20.412675616356104	25.67497941084592	31.673218683186672	22.239126289611303
19	20.579011639464643	27.648292621577436	28.63900620311657	23.133689535841356
20	23.686655388272904	27.013781548060965	30.456531528790332	18.843031534875795
21	20.9104665928297	29.992819152173116	29.666232570216117	19.430481684781064
22	18.659656211839067	25.313908531415198	30.22568958452507	25.800745672220664
23	18.578516688371487	29.02766452052627	28.824815711857326	23.569003079244915
24	22.356372901021953	24.27694542149954	29.51125608039304	23.85542559708547
25	18.742824223393338	30.069901699467316	27.367144172762277	23.820129904377072
26	19.987504513385993	27.923355606132528	28.852403149836302	23.23673673064518
27	22.230200942029867	28.83779803561214	27.896173865770884	21.035827156587107
28	17.410918945673032	26.982948529143286	33.843700935944405	21.762431589239277
29	22.61439658564885	23.454190653538294	29.752240465091752	24.17917229572111
30	22.97709025554893	26.610112418809766	28.684444336258412	21.72835298938289
31	23.266758354328186	26.72046217072567	26.600375675993654	23.41240379895249
32	24.16132160055824	29.00494545395535	24.540243175151833	22.293489770334578
33	21.318192698254283	26.193866663421083	26.49732848118983	25.9906121571348
34	22.288215701309184	27.08680711918179	27.30344964684022	23.3215275326688
35	21.364036529013465	27.48357938893825	25.616964651566605	25.53541943048169
36	24.138602533987317	25.04330822065082	29.86745858841571	20.95063065694615
37	21.350648507641314	27.12169711427285	30.638284061357705	20.88937031672813
38	18.685215161731357	26.302187927250305	27.598391814644874	27.41420509637347
39	20.88977601434547	25.842938224423804	28.75219583835384	24.515089922876886
40	20.80052253853113	27.008507479035575	28.356234963832055	23.834735018601236
41	18.918896989317982	28.59843644138278	28.355829266214723	24.12683730308452
42	21.291822353127323	26.20968887049726	30.188365403729982	22.31012337264543
43	22.83631318233268	23.508148436644234	29.70761372718458	23.94792465383851
44	20.58671989419406	24.816928950176276	29.455675506817748	25.140675648811918
45	19.631707702980663	24.702116524469652	27.40974242258275	28.256433349966937
46	24.755262912340918	26.070534587750366	27.277079301713258	21.897123198195455
47	18.580950874075516	25.311474345711165	33.792583036159826	22.314991744053486
48	20.604976286974267	24.608400374864598	29.881658005022537	24.9049653331386
49	20.93886542604335	21.39365245507913	33.72036886027369	23.94711325860383
50	20.946573680772772	22.426558588821408	31.086174230898745	25.54069349950708
51	19.775324659518276	23.778343049791268	26.74886100393932	29.69747128675113
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	486.0
1	485.5
2	485.0
3	715.5
4	946.0
5	693.0
6	440.0
7	468.5
8	497.0
9	513.0
10	529.0
11	590.5
12	652.0
13	573.0
14	494.0
15	512.5
16	531.0
17	513.0
18	495.0
19	594.0
20	693.0
21	669.5
22	646.0
23	802.0
24	958.0
25	1274.0
26	1677.5
27	1765.0
28	2164.0
29	2563.0
30	3215.5
31	3868.0
32	4021.0
33	4174.0
34	4924.5
35	5675.0
36	6465.0
37	7255.0
38	7840.5
39	8426.0
40	9886.0
41	11346.0
42	13307.0
43	15268.0
44	17495.5
45	19723.0
46	28259.0
47	36795.0
48	32553.5
49	28312.0
50	27430.0
51	26548.0
52	22233.5
53	17919.0
54	15362.0
55	12805.0
56	11558.5
57	10312.0
58	8950.5
59	7589.0
60	7109.0
61	6629.0
62	5482.0
63	4335.0
64	3581.5
65	2828.0
66	2106.0
67	1384.0
68	1039.0
69	694.0
70	506.5
71	319.0
72	275.0
73	231.0
74	165.5
75	94.5
76	89.0
77	71.5
78	54.0
79	38.5
80	23.0
81	17.0
82	11.0
83	6.0
84	1.0
85	2.5
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	246489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.32071208045795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95408387412606	23.902486520696662
2	10.759787615951398	6.955279951640844
3	3.945171777523943	3.825322833878997
4	1.855222363086347	2.3984843137016254
5	1.1171501374471238	1.8053543971536257
6	0.7669423977305534	1.487287465160717
7	0.5410019204940565	1.2239897115084244
8	0.4430943803582412	1.145690071362211
9	0.45815707884067425	1.3327166729549798
>10	5.951021125434622	43.12159974684469
>50	0.1456060853301869	2.888161337828463
>100	0.048953770067907666	2.834203554722523
>500	0.007531349241216564	1.6085910527447473
>1k	0.00627612436768047	5.470832369801492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGC	3967	1.6094024479794231	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCG	3617	1.4674082819111602	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTC	2995	1.2150643639269907	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	1576	0.6393794449245199	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	1330	0.5395778310593982	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	775	0.3144156534368674	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCT	771	0.3127928629675158	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCCAATT	708	0.2872339130752285	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTA	616	0.24990973228014232	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTC	551	0.22353938715317925	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCT	544	0.22069950383181403	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	500	0.2028488086689467	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	483	0.1959519491742025	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC	409	0.16593032549119838	No Hit
ACTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	333	0.1350973065735185	No Hit
TTTTATGGGTTTATGAAAAGTTAGTGTGGTGTTAACACTAAAGTAGGTGGG	304	0.12333207567071958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0569761733789337E-4	0.0	0.0	0.33145495336505887	0.0
2	4.0569761733789337E-4	0.0	0.0	1.9603308869767009	0.0
3	4.0569761733789337E-4	0.0	0.0	2.5408841773872264	0.0
4	4.0569761733789337E-4	0.0	0.0	3.427333471270523	0.0
5	4.0569761733789337E-4	0.0	0.0	6.1844544786988465	0.0
6	4.0569761733789337E-4	0.0	0.0	7.2360227028386666	0.0
7	4.0569761733789337E-4	0.0	0.0	8.468532064311185	0.0
8	4.0569761733789337E-4	0.0	0.0	10.02600521727136	0.0
9	4.0569761733789337E-4	0.0	0.0	10.545703865081201	0.0
10	4.0569761733789337E-4	0.0	0.0	13.171378844492047	0.0
11	4.0569761733789337E-4	0.0	0.0	14.839201749368126	0.0
12	4.0569761733789337E-4	0.0	0.0	17.7906519155013	0.0
13	4.0569761733789337E-4	0.0	0.0	18.49210309587852	0.0
14	4.0569761733789337E-4	0.0	0.0	18.796782006499274	0.0
15	8.113952346757867E-4	0.0	0.0	19.457663425142705	0.0
16	8.113952346757867E-4	0.0	0.0	20.346952602347365	0.0
17	8.113952346757867E-4	0.0	0.0	21.364036529013465	0.0
18	8.113952346757867E-4	0.0	0.0	22.558004616838886	0.0
19	8.113952346757867E-4	0.0	0.0	23.524782038955085	0.0
20	8.113952346757867E-4	0.0	0.0	24.240838333556468	0.0
21	8.113952346757867E-4	0.0	0.0	25.049799382528228	0.0
22	8.113952346757867E-4	0.0	0.0	25.903792867024492	0.0
23	8.113952346757867E-4	0.0	0.0	26.649870785308877	0.0
24	8.113952346757867E-4	0.0	0.0	27.238132330448824	0.0
25	8.113952346757867E-4	0.0	0.0	27.73551760930508	0.0
26	8.113952346757867E-4	0.0	0.0	28.197607195452942	0.0
27	8.113952346757867E-4	0.0	0.0	28.735562236042988	0.0
28	8.113952346757867E-4	0.0	0.0	29.23051332919522	0.0
29	8.113952346757867E-4	0.0	0.0	29.74047523418895	0.0
30	8.113952346757867E-4	0.0	0.0	30.350238753047805	0.0
31	8.113952346757867E-4	0.0	0.0	30.92064960302488	0.0
32	8.113952346757867E-4	0.0	0.0	31.416817789029125	0.0
33	8.113952346757867E-4	0.0	0.0	31.903654929834598	0.0
34	8.113952346757867E-4	0.0	0.0	32.41077695150696	0.0
35	8.113952346757867E-4	0.0	0.0	32.930881296934146	0.0
36	8.113952346757867E-4	0.0	0.0	33.408793090158184	0.0
37	8.113952346757867E-4	0.0	0.0	33.90861255471847	0.0
38	8.113952346757867E-4	0.0	0.0	34.39666678837595	0.0
39	0.0012170928520136802	0.0	0.0	34.905817298135005	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.2067358E-7	45.000004	1
ACGGGTT	35	1.2067358E-7	45.000004	5
AACGGGT	35	1.2067358E-7	45.000004	4
TCACGAG	25	3.8818605E-5	45.0	32
ACAACGT	25	3.8818605E-5	45.0	41
CTTCGAT	20	7.0224935E-4	45.0	19
CGGGTCA	25	3.8818605E-5	45.0	6
AGACTGA	25	3.8818605E-5	45.0	41
AACAACG	20	7.0224935E-4	45.0	40
ACCGGCC	20	7.0224935E-4	45.0	31
TCCGCTA	20	7.0224935E-4	45.0	26
CACGGTT	20	7.0224935E-4	45.0	30
GATCGAG	20	7.0224935E-4	45.0	1
CAACGTA	25	3.8818605E-5	45.0	42
CCCTATG	20	7.0224935E-4	45.0	16
ACTTGCG	25	3.8818605E-5	45.0	16
CATTGCG	25	3.8818605E-5	45.0	24
TCGGGTA	25	3.8818605E-5	45.0	5
ATAGCGG	40	6.7775545E-9	45.0	2
GCACGGT	20	7.0224935E-4	45.0	29
>>END_MODULE