##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935639.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698950
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.012039487803136	33.0	31.0	34.0	30.0	34.0
2	32.03912153945203	33.0	31.0	34.0	30.0	34.0
3	32.070039344731384	33.0	31.0	34.0	30.0	34.0
4	35.735018241648184	37.0	35.0	37.0	35.0	37.0
5	35.74697188640103	37.0	35.0	37.0	35.0	37.0
6	35.64971314114028	37.0	35.0	37.0	33.0	37.0
7	35.88718220187424	37.0	35.0	37.0	35.0	37.0
8	35.58825237856785	37.0	35.0	37.0	33.0	37.0
9	37.391538736676445	39.0	37.0	39.0	34.0	39.0
10	37.16903355032549	39.0	37.0	39.0	34.0	39.0
11	37.174813649045	39.0	37.0	39.0	34.0	39.0
12	37.10862579583661	39.0	37.0	39.0	33.0	39.0
13	36.965257886830244	39.0	37.0	39.0	33.0	39.0
14	38.170276843837186	40.0	37.0	41.0	33.0	41.0
15	38.12852278417626	40.0	37.0	41.0	33.0	41.0
16	38.115162744116176	40.0	37.0	41.0	33.0	41.0
17	37.98569997853924	40.0	37.0	41.0	33.0	41.0
18	37.95261320552257	40.0	37.0	41.0	33.0	41.0
19	37.80280134487445	40.0	37.0	41.0	33.0	41.0
20	37.719583661206094	40.0	36.0	41.0	32.0	41.0
21	37.918962729808996	40.0	36.0	41.0	33.0	41.0
22	37.9650075112669	40.0	37.0	41.0	33.0	41.0
23	37.944961728306744	40.0	37.0	41.0	33.0	41.0
24	37.88370841977252	40.0	36.0	41.0	33.0	41.0
25	37.787232276986906	40.0	36.0	41.0	33.0	41.0
26	37.73074754989627	40.0	36.0	41.0	33.0	41.0
27	37.65735174189856	40.0	36.0	41.0	33.0	41.0
28	37.50377995564776	40.0	36.0	41.0	33.0	41.0
29	37.46389298233064	40.0	36.0	41.0	32.0	41.0
30	37.39050718935546	40.0	36.0	41.0	32.0	41.0
31	37.36158237356034	40.0	36.0	41.0	32.0	41.0
32	37.198762429358325	40.0	36.0	41.0	32.0	41.0
33	37.066649974962445	40.0	35.0	41.0	31.0	41.0
34	36.97513126833107	40.0	35.0	41.0	31.0	41.0
35	36.82599184491022	40.0	35.0	41.0	31.0	41.0
36	36.837815294370124	40.0	35.0	41.0	31.0	41.0
37	36.70134201301953	40.0	35.0	41.0	31.0	41.0
38	36.63345446741541	40.0	35.0	41.0	30.0	41.0
39	36.530122326346664	39.0	35.0	41.0	30.0	41.0
40	36.397014092567424	39.0	35.0	41.0	30.0	41.0
41	36.14367837470491	39.0	35.0	41.0	28.0	41.0
42	36.223619715287214	39.0	35.0	41.0	29.0	41.0
43	36.22004721367766	39.0	35.0	41.0	30.0	41.0
44	36.1975992560269	39.0	35.0	41.0	30.0	41.0
45	36.21393804993204	39.0	35.0	41.0	30.0	41.0
46	36.04806495457472	39.0	35.0	41.0	29.0	41.0
47	35.78389584376565	38.0	35.0	40.0	28.0	41.0
48	35.791092352814935	38.0	35.0	40.0	29.0	41.0
49	35.733068173689105	38.0	35.0	40.0	29.0	41.0
50	35.59463337863939	38.0	35.0	40.0	28.0	41.0
51	34.44939838328922	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	12.0
11	14.0
12	14.0
13	12.0
14	16.0
15	19.0
16	47.0
17	104.0
18	249.0
19	596.0
20	1328.0
21	2498.0
22	3338.0
23	4139.0
24	4957.0
25	5849.0
26	6571.0
27	7049.0
28	7493.0
29	8415.0
30	10400.0
31	13617.0
32	18164.0
33	24583.0
34	40476.0
35	53793.0
36	50719.0
37	72778.0
38	128149.0
39	233374.0
40	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.252521639602264	15.598826811646042	19.15616281565205	14.992488733099648
2	26.362257672222622	34.66070534372988	22.04306459689534	16.933972387152156
3	28.35424565419558	33.536161384934545	23.211531583088917	14.898061377780959
4	25.453752056656416	22.377280206023322	34.32548823234852	17.843479504971743
5	32.395450318334646	25.853208384004578	22.19257457614994	19.558766721510835
6	22.165534015308676	40.17655054009586	23.278203018813933	14.379712425781529
7	71.7386079118678	5.408827527004792	18.193862221904286	4.658702339223121
8	70.69518563559625	13.957793833607553	10.096573431575935	5.250447099220259
9	65.24615494670577	7.7732312754846555	10.688032048072108	16.292581729737464
10	39.633736318763866	27.387509836182844	18.03390800486444	14.944845840188856
11	33.67622862865727	24.18813935188497	23.963373631876387	18.172258387581373
12	28.960297589241	19.749195221403536	27.352600329065023	23.937906860290436
13	26.813649044996062	22.040060090135203	32.064382287717294	19.08190857715144
14	20.043493812146792	30.963159024250665	25.6626368123614	23.330710351241144
15	17.148580012876458	25.40424923098934	35.78853995278632	21.65863080334788
16	19.199942771299806	25.22498032763431	29.104084698476285	26.4709922025896
17	19.0179555046856	26.97846770155233	30.416911080907077	23.586665712854995
18	20.778596466127762	25.466628514199876	27.300236068388294	26.454538951284068
19	21.914443093211244	27.785249302525216	24.74039630874884	25.5599112955147
20	27.734458831103797	24.892624651262608	29.201087345303673	18.17182917232992
21	24.391444309321127	30.697903998855423	25.27519851205379	19.635453179769655
22	21.057729451319837	23.759639459188783	29.093640460691038	26.088990628800346
23	23.74404463838615	29.472494455969667	26.923814292867874	19.85964661277631
24	23.283639745332284	24.731382788468416	26.55497532012304	25.43000214607626
25	20.994062522354962	32.63852922240504	23.539165891694687	22.828242363545318
26	22.14965305100508	25.486515487516993	27.89956363116103	24.464267830316906
27	26.018742399313254	28.056656413191217	23.757779526432508	22.166821661063025
28	20.058230202446527	29.6275842334931	28.567851777666498	21.746333786393876
29	25.778524930252523	25.211960798340368	26.08198011302668	22.92753415838043
30	21.370627369625865	31.311109521424996	25.952357107089206	21.365906001859933
31	23.260175978253095	29.144144788611488	22.000286143500965	25.59539308963445
32	26.040060090135203	29.5290077974104	22.11617426139209	22.314757851062307
33	22.79490664568281	28.01030116603477	21.598826811646042	27.595965376636382
34	25.536590600185992	28.85628442663996	23.463194792188283	22.143930180985762
35	24.787753058158664	27.466199298948425	22.95715001073038	24.78889763216253
36	22.897489090779025	33.76078403319265	21.872666142070248	21.46906073395808
37	20.74898061377781	30.916231490092283	26.23005937477645	22.104728521353458
38	21.50196723656914	29.071178195865226	22.60619500679591	26.820659560769727
39	23.685814435939623	27.515415981114526	23.72215466056227	25.076614922383577
40	24.655554760712498	23.87123542456542	28.176264396594892	23.29694541812719
41	19.453322841404965	32.26010444237785	25.273481651048	23.013091065169185
42	24.467987695829457	26.35138421918592	27.72501609557193	21.45561198941269
43	24.39273195507547	28.600615208527074	23.358037055583374	23.64861578081408
44	23.12869303955934	26.26539809714572	26.282709778954143	24.323199084340796
45	22.01602403605408	23.966664282137494	25.149438443379356	28.86787323842907
46	25.089205236426064	28.67157879676658	23.273195507547033	22.96602045926032
47	20.2045926031905	26.48301022963016	31.433292796337366	21.87910437084198
48	22.756277273052437	26.945275055440305	25.373059589384077	24.925388082123185
49	21.091065169182343	24.921239001359183	30.01058730953573	23.97710851992274
50	20.90979326132055	25.230703197653625	27.944631232563133	25.914872308462694
51	21.080477859646614	24.35581944345089	25.09321124543959	29.47049145146291
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	493.0
1	924.0
2	1355.0
3	2757.0
4	4159.0
5	2921.0
6	1683.0
7	1610.0
8	1537.0
9	1530.5
10	1524.0
11	1503.5
12	1483.0
13	1498.5
14	1514.0
15	1511.0
16	1508.0
17	1660.5
18	1813.0
19	1742.5
20	1672.0
21	1925.5
22	2179.0
23	2302.0
24	2425.0
25	3128.0
26	4656.5
27	5482.0
28	6190.5
29	6899.0
30	8546.5
31	10194.0
32	11906.0
33	13618.0
34	15557.0
35	17496.0
36	18619.0
37	19742.0
38	21492.0
39	23242.0
40	25014.0
41	26786.0
42	29515.0
43	32244.0
44	35100.0
45	37956.0
46	72268.0
47	106580.0
48	81924.0
49	57268.0
50	56401.5
51	55535.0
52	49670.0
53	43805.0
54	40843.5
55	37882.0
56	35992.5
57	34103.0
58	33397.0
59	32691.0
60	33367.0
61	34043.0
62	30918.5
63	27794.0
64	22490.0
65	17186.0
66	14032.5
67	10879.0
68	8904.0
69	6929.0
70	5986.0
71	5043.0
72	4251.5
73	3460.0
74	2793.5
75	1840.5
76	1554.0
77	1144.5
78	735.0
79	508.5
80	282.0
81	188.5
82	95.0
83	86.5
84	78.0
85	53.5
86	29.0
87	20.0
88	11.0
89	7.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	698950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.816380778340655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74030993398677	19.77448229902645
2	8.391549244814238	4.500619597382711
3	3.0502192856366572	2.4538752546321243
4	1.7658481700523383	1.8941462769943813
5	1.26644364586975	1.698071752097661
6	0.9816049889826407	1.5793855895086428
7	0.7975390256486667	1.4970977138167008
8	0.7548921754322727	1.6194780818385404
9	0.6514954014495203	1.5723673884547418
>10	8.317456762147593	46.3897196692184
>50	0.21774647929358842	3.61999930719987
>100	0.05524110188974509	2.658405530165857
>500	0.0026816068878517036	0.4416773091938621
>1k	0.005363213775703407	3.550101823556266
>5k	0.0	0.0
>10k+	0.0016089641327110221	6.750572406913808
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	16663	2.3840045782960155	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	15353	2.1965805851634594	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	14921	2.134773588954861	No Hit
GAACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCT	4062	0.5811574504614064	No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	3768	0.5390943558194434	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT	2964	0.4240646684312182	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTC	2675	0.38271693254166966	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACCAGAT	2601	0.37212962300593744	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2341	0.334930967880392	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTA	2132	0.30502897202947277	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTT	1910	0.27326704342227626	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1130	0.16167107804563988	No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1101	0.15752199728163674	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	760	0.10873453036697904	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.722870019314686E-4	0.0	0.0	0.11846340939981401	0.0
2	5.722870019314686E-4	0.0	0.0	0.9424136204306459	0.0
3	5.722870019314686E-4	0.0	0.0	1.1751913584662708	0.0
4	5.722870019314686E-4	0.0	0.0	2.015165605551184	0.0
5	5.722870019314686E-4	0.0	0.0	5.011517275913871	0.0
6	5.722870019314686E-4	0.0	0.0	5.775091208240933	0.0
7	5.722870019314686E-4	0.0	0.0	6.610487159310394	0.0
8	5.722870019314686E-4	0.0	0.0	7.622004435224265	0.0
9	5.722870019314686E-4	0.0	0.0	7.915730738965591	0.0
10	5.722870019314686E-4	0.0	0.0	10.606195006795907	0.0
11	5.722870019314686E-4	0.0	0.0	11.282065956076973	0.0
12	5.722870019314686E-4	0.0	0.0	14.144073252736247	0.0
13	5.722870019314686E-4	0.0	0.0	14.521353458759569	0.0
14	5.722870019314686E-4	0.0	0.0	14.725230703197653	0.0
15	5.722870019314686E-4	0.0	0.0	15.218255955361613	0.0
16	5.722870019314686E-4	0.0	0.0	15.614564704199156	0.0
17	5.722870019314686E-4	0.0	0.0	16.035195650618785	0.0
18	5.722870019314686E-4	0.0	0.0	16.4801487946205	0.0
19	5.722870019314686E-4	0.0	0.0	17.167894699191645	0.0
20	5.722870019314686E-4	0.0	0.0	17.51112382860004	0.0
21	5.722870019314686E-4	0.0	0.0	17.84333643322126	0.0
22	7.153587524143358E-4	0.0	0.0	18.31776235782245	0.0
23	7.153587524143358E-4	0.0	0.0	18.66671435725016	0.0
24	7.153587524143358E-4	0.0	0.0	18.976178553544603	0.0
25	7.153587524143358E-4	0.0	0.0	19.2434365834466	0.0
26	7.153587524143358E-4	0.0	0.0	19.492381429286787	0.0
27	7.153587524143358E-4	0.0	0.0	19.812289863366477	0.0
28	7.153587524143358E-4	0.0	0.0	20.07139280349095	0.0
29	7.153587524143358E-4	0.0	0.0	20.33979540739681	0.0
30	7.153587524143358E-4	0.0	0.0	20.704342227627155	0.0
31	8.584305028972029E-4	0.0	0.0	20.989198082838545	0.0
32	8.584305028972029E-4	0.0	0.0	21.28836111309822	0.0
33	8.584305028972029E-4	0.0	0.0	21.563488089276774	0.0
34	8.584305028972029E-4	0.0	0.0	21.84390872022319	0.0
35	8.584305028972029E-4	0.0	0.0	22.140067243722726	0.0
36	8.584305028972029E-4	0.0	0.0	22.4292152514486	0.0
37	8.584305028972029E-4	0.0	0.0	22.739967093497388	0.0
38	0.00100150225338007	0.0	0.0	23.01995850919236	0.0
39	0.00100150225338007	0.0	0.0	23.316975463194794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	45	3.8380676E-10	45.0	1
CTAAGCG	40	6.8084773E-9	45.0	1
GTATGCG	20	7.031387E-4	45.0	1
CGTACAG	20	7.031387E-4	45.0	24
AGTTACG	20	7.031387E-4	45.0	1
CGGTCTA	40	6.8084773E-9	45.0	31
GCTACGA	90	0.0	45.0	10
CAGTACG	20	7.031387E-4	45.0	1
CGATCGA	70	0.0	45.0	41
TACACCG	20	7.031387E-4	45.0	1
CGATGAA	1320	0.0	43.125	19
GATGAAT	1395	0.0	42.580643	20
CCGATGA	1320	0.0	42.102272	18
TACGGCT	1665	0.0	40.81081	7
ACCGATG	1390	0.0	40.305756	17
ATGAATG	1475	0.0	39.813557	21
ACGGCTG	1710	0.0	39.605263	8
CACCGAT	1530	0.0	39.558823	16
GATACCT	1610	0.0	39.40994	5
TTGCGAG	40	3.455625E-7	39.375	1
>>END_MODULE