FastQCFastQC Report
Sat 14 Jan 2017
SRR2935637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514813
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC73191.4216812706749828No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG68871.337767305798416No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC57051.1081693741222542No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC21530.41821010735937125No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT15810.30710180201354664No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC13420.26067717792674233No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT12600.24474906422331993No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.2307634034105588No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT10300.20007264773811073No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA10030.1948280249333253No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC9310.18084236412056418No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT7180.13946811754947913No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTT7010.13616594763535497No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6320.12276302268979221No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5390.10469821080664242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGG453.8380676E-1045.0000042
ACAACGA351.2101737E-745.013
AACGGTA253.887795E-545.011
TGATTCC207.029654E-445.019
GCGATTA406.8030204E-945.09
CGTACAT207.029654E-445.035
ACGTAGT207.029654E-445.039
GCGTGAT207.029654E-445.016
CTTTACG253.887795E-545.01
CGGTATT253.887795E-545.013
GACGAAA207.029654E-445.041
GGTTCGT207.029654E-445.036
TACACGA253.887795E-545.014
GAGTACG302.1631586E-644.9999961
CACAACG302.1631586E-644.99999612
TACGGCT8450.041.272197
TTACGGG1200.041.2499963
CCGATGA5700.040.65789418
CAATCGG501.0786607E-940.52
TTAGCGG900.040.0000042
Produced by FastQC (version 0.11.5)