##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188462
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.080743067568	33.0	31.0	34.0	30.0	34.0
2	32.08750835712239	33.0	31.0	34.0	30.0	34.0
3	32.10914667147754	33.0	31.0	34.0	30.0	34.0
4	35.75712345194257	37.0	35.0	37.0	33.0	37.0
5	35.83820080440619	37.0	35.0	37.0	35.0	37.0
6	35.790652757585086	37.0	35.0	37.0	35.0	37.0
7	36.06257494879604	37.0	35.0	37.0	35.0	37.0
8	35.90034065222697	37.0	35.0	37.0	35.0	37.0
9	37.728894949645024	39.0	38.0	39.0	35.0	39.0
10	37.34137916396939	39.0	37.0	39.0	34.0	39.0
11	37.32965796818457	39.0	37.0	39.0	34.0	39.0
12	37.07986755950802	39.0	35.0	39.0	34.0	39.0
13	36.840551410894506	39.0	35.0	39.0	33.0	39.0
14	37.898181065679026	40.0	36.0	41.0	33.0	41.0
15	38.07589328352665	40.0	36.0	41.0	33.0	41.0
16	37.94287442561365	40.0	36.0	41.0	33.0	41.0
17	37.98778533603591	40.0	36.0	41.0	34.0	41.0
18	37.93642750262652	40.0	36.0	41.0	34.0	41.0
19	37.830114293597646	40.0	36.0	41.0	34.0	41.0
20	37.95666500408571	40.0	36.0	41.0	34.0	41.0
21	37.890848022413	40.0	35.0	41.0	34.0	41.0
22	37.986782481349024	40.0	35.0	41.0	34.0	41.0
23	38.02369708482347	40.0	35.0	41.0	34.0	41.0
24	37.94963971516805	40.0	35.0	41.0	34.0	41.0
25	37.733887998641634	40.0	35.0	41.0	34.0	41.0
26	37.801530281966656	40.0	35.0	41.0	34.0	41.0
27	37.780321762477314	40.0	35.0	41.0	34.0	41.0
28	37.70442317284121	40.0	35.0	41.0	34.0	41.0
29	37.70135624157655	40.0	35.0	41.0	34.0	41.0
30	37.50663794292748	39.0	35.0	41.0	33.0	41.0
31	37.48690452186648	39.0	35.0	41.0	33.0	41.0
32	37.418370812153114	40.0	35.0	41.0	33.0	41.0
33	37.309600874446836	39.0	35.0	41.0	33.0	41.0
34	37.2826988995129	40.0	35.0	41.0	33.0	41.0
35	37.16986448196453	39.0	35.0	41.0	33.0	41.0
36	37.067621058887205	39.0	35.0	41.0	33.0	41.0
37	37.027321157580836	39.0	35.0	41.0	32.0	41.0
38	36.935551994566545	39.0	35.0	41.0	32.0	41.0
39	36.95559316997591	39.0	35.0	41.0	32.0	41.0
40	36.83821672273456	39.0	35.0	41.0	32.0	41.0
41	36.71129989069414	39.0	35.0	41.0	31.0	41.0
42	36.75269285054812	39.0	35.0	41.0	32.0	41.0
43	36.669376319894724	39.0	35.0	41.0	31.0	41.0
44	36.55386762318133	39.0	35.0	41.0	31.0	41.0
45	36.55837781621759	39.0	35.0	41.0	31.0	41.0
46	36.428383440693615	39.0	35.0	41.0	31.0	41.0
47	36.32432532818287	38.0	35.0	41.0	31.0	41.0
48	36.261039360719934	38.0	35.0	40.0	31.0	41.0
49	36.24549776612792	38.0	35.0	40.0	31.0	41.0
50	36.104848722819455	38.0	35.0	40.0	31.0	41.0
51	34.939818106567905	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	3.0
11	4.0
12	4.0
13	2.0
14	4.0
15	4.0
16	16.0
17	26.0
18	50.0
19	73.0
20	172.0
21	232.0
22	334.0
23	475.0
24	797.0
25	1023.0
26	1378.0
27	1570.0
28	1756.0
29	2106.0
30	2622.0
31	3458.0
32	4591.0
33	6471.0
34	13815.0
35	20230.0
36	12310.0
37	17968.0
38	34720.0
39	62212.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.551814158822474	19.70370684806486	23.08529040337044	14.659188589742229
2	28.276787893580668	26.350670161624095	26.98103596480988	18.391505979985357
3	31.037026031772985	25.603039339495492	27.484055141089453	15.875879487642072
4	26.644098014453842	24.986469420891215	29.742865935838523	18.62656662881642
5	25.466672326516754	29.473315575553695	26.340588553660687	18.71942354426887
6	24.67128651929832	34.86485339219577	25.373284800118856	15.090575288387049
7	77.30099436491176	6.56843289363373	11.278135645382093	4.852437096072418
8	78.35903259012427	6.939329944498095	9.197610128301728	5.504027337075909
9	73.83292122549904	6.7727181076291245	11.261686706073373	8.132673960798463
10	41.4407148390657	24.398552493340834	19.08713692946058	15.073595738132886
11	34.94603686684849	24.872918678566503	22.521250968364974	17.659793486220035
12	33.4682853837909	21.95243603485053	26.214303148645353	18.364975432713226
13	25.417856119536037	27.455932760980993	27.455932760980993	19.67027835850198
14	18.64885228852501	32.49249185512198	26.609608303000076	22.24904755335293
15	16.7535099914041	27.154015133024163	38.335048975390265	17.75742590018147
16	19.92921649987796	23.832390614553596	35.26493404505948	20.97345884050896
17	19.716972121700927	23.952839299169064	28.95490868185629	27.375279897273717
18	21.890354554233745	25.64867188080356	29.95988581252454	22.501087752438156
19	25.680508537530113	27.42993282465431	26.024344430176903	20.865214207638676
20	28.219481911472872	26.54540437860152	26.740138595578948	18.49497511434666
21	24.984346977109446	28.274665449798896	26.66054695376256	20.080440619329096
22	20.762806295168257	26.400547590495698	27.15348452207872	25.683161592257324
23	21.565620655622883	28.42323651452282	26.401608812386584	23.609534017467713
24	23.802676401608814	23.614309515976693	28.290583778162176	24.292430304252317
25	20.60892912098991	26.872260720994152	28.339399985142894	24.179410172873045
26	20.470439664229396	29.542294998461227	27.22511699971347	22.762148337595907
27	22.537699907673694	29.421846313845762	27.980706986023705	20.059746792456835
28	20.559051692118306	27.94993155118804	30.926658955120928	20.56435780157273
29	22.94998461228258	26.565036983582896	29.209071324723286	21.275907079411237
30	22.49365919920196	27.654911865521964	28.65564410862667	21.195784826649405
31	26.31936411584298	26.74597531597882	24.271205866434613	22.66345470174359
32	28.222665577145523	28.880623149494326	23.64455433986692	19.252156933493225
33	25.017775466672326	28.776092793242142	24.771571987986967	21.43455975209857
34	23.768186690155044	27.133851917097346	26.264180577516953	22.833780815230657
35	21.730640659655528	27.799238042682344	26.149568613301355	24.32055268436077
36	24.83524530144008	28.345236705542764	26.21058887202725	20.60892912098991
37	23.772431577718585	29.30405068395751	28.28793072343496	18.635587014888944
38	22.619944604217295	30.51808852713014	24.003247338986107	22.85871952966646
39	23.947002578769194	26.89348515881186	25.77867156243699	23.38084069998196
40	23.89871698273392	25.82642654752682	28.513440375248063	21.7614160944912
41	18.93485158811856	27.76156466555592	27.420912438581784	25.88267130774374
42	23.259861404421052	27.476626587853254	26.710954993579605	22.55255701414609
43	24.629368254608355	27.078137767825876	26.412751642240877	21.87974233532489
44	23.23333085714892	26.99058696182785	26.44989440842186	23.326187772601372
45	22.381169678768135	26.629771518926894	25.278836051830076	25.710222750474898
46	22.907005125701733	28.152624932347102	25.838099988326558	23.102269953624603
47	21.048274983816366	27.274994428585075	29.62719274973204	22.04953783786652
48	22.608801774363002	25.12602009954261	27.725483121265825	24.53969500482856
49	21.80333435918116	24.64051108446265	30.040538676231815	23.515615880124376
50	20.647663720007216	26.044507646103725	27.998747758168758	25.3090808757203
51	19.77905360231771	26.43397608005858	25.665120820112275	28.121849497511437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	658.0
1	552.0
2	446.0
3	603.0
4	760.0
5	556.0
6	352.0
7	403.5
8	455.0
9	450.5
10	446.0
11	491.5
12	537.0
13	494.0
14	451.0
15	462.0
16	473.0
17	451.5
18	430.0
19	483.0
20	536.0
21	496.5
22	457.0
23	552.5
24	648.0
25	691.0
26	960.0
27	1186.0
28	1438.0
29	1690.0
30	2085.5
31	2481.0
32	2799.0
33	3117.0
34	3591.5
35	4066.0
36	4463.0
37	4860.0
38	5341.0
39	5822.0
40	6900.5
41	7979.0
42	9877.0
43	11775.0
44	13402.0
45	15029.0
46	17989.0
47	20949.0
48	19906.5
49	18864.0
50	18734.5
51	18605.0
52	16563.0
53	14521.0
54	12475.0
55	10429.0
56	9960.5
57	9492.0
58	9025.5
59	8559.0
60	8090.0
61	7621.0
62	6740.5
63	5860.0
64	4836.0
65	3812.0
66	2884.5
67	1957.0
68	1519.5
69	1082.0
70	866.5
71	651.0
72	518.5
73	386.0
74	261.0
75	107.5
76	79.0
77	70.0
78	61.0
79	31.0
80	1.0
81	0.5
82	0.0
83	1.5
84	3.0
85	2.0
86	1.0
87	1.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	188462.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.50947140537615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9298165437096	24.35928728337808
2	9.608604818175882	6.247413271640967
3	3.305151139257035	3.223461493563689
4	1.6387020957106484	2.1309335568974115
5	1.0576483645622512	1.7191794632339676
6	0.7818110596069726	1.5249758572019825
7	0.5761572109420905	1.3111396461886216
8	0.5304563556832278	1.3795884581507147
9	0.4733302866096494	1.3848945676051405
>10	6.892668277077757	48.80453353991786
>50	0.1615851668081217	3.147053517419957
>100	0.03427564144414703	2.038607252390402
>500	0.008160867010511197	2.1824028186053424
>1k	0.001632173402102239	0.54652927380586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	1030	0.54652927380586	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	977	0.5184068936974032	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	967	0.5131007842429773	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	829	0.4398764737719009	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	814	0.43191730959026226	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	526	0.27910135730279845	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	340	0.18040772145047806	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC	337	0.17881588861415032	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	317	0.16820366970529868	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	316	0.16767305875985608	No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATATA	194	0.10293852341586103	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT	193	0.10240791247041844	No Hit
ACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	191	0.10134669057953329	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	189	0.10028546868864811	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.306109454425826E-4	0.0	0.0	0.2966115185024037	0.0
2	5.306109454425826E-4	0.0	0.0	1.5897103925459775	0.0
3	5.306109454425826E-4	0.0	0.0	2.107586675297938	0.0
4	5.306109454425826E-4	0.0	0.0	2.7029321560845156	0.0
5	5.306109454425826E-4	0.0	0.0	4.416805509864058	0.0
6	5.306109454425826E-4	0.0	0.0	5.252517748936125	0.0
7	5.306109454425826E-4	0.0	0.0	6.154025745243072	0.0
8	5.306109454425826E-4	0.0	0.0	7.496471437212807	0.0
9	5.306109454425826E-4	0.0	0.0	7.954919294075198	0.0
10	5.306109454425826E-4	0.0	0.0	9.450180938332396	0.0
11	5.306109454425826E-4	0.0	0.0	10.856830554700682	0.0
12	5.306109454425826E-4	0.0	0.0	12.44654094724666	0.0
13	5.306109454425826E-4	0.0	0.0	12.934172406108393	0.0
14	5.306109454425826E-4	0.0	0.0	13.124661735522281	0.0
15	5.306109454425826E-4	0.0	0.0	13.510946503804481	0.0
16	5.306109454425826E-4	0.0	0.0	14.210822340843247	0.0
17	5.306109454425826E-4	0.0	0.0	15.060330464496822	0.0
18	5.306109454425826E-4	0.0	0.0	16.196899109634835	0.0
19	5.306109454425826E-4	0.0	0.0	16.799673143657607	0.0
20	5.306109454425826E-4	0.0	0.0	17.34673302840891	0.0
21	5.306109454425826E-4	0.0	0.0	17.927221402723095	0.0
22	5.306109454425826E-4	0.0	0.0	18.591546306417207	0.0
23	5.306109454425826E-4	0.0	0.0	19.17893262302215	0.0
24	5.306109454425826E-4	0.0	0.0	19.664972249047555	0.0
25	5.306109454425826E-4	0.0	0.0	20.07725695365644	0.0
26	5.306109454425826E-4	0.0	0.0	20.460358056265985	0.0
27	5.306109454425826E-4	0.0	0.0	20.8705203170931	0.0
28	5.306109454425826E-4	0.0	0.0	21.2891723530473	0.0
29	5.306109454425826E-4	0.0	0.0	21.71896721885579	0.0
30	5.306109454425826E-4	0.0	0.0	22.219863951353588	0.0
31	5.306109454425826E-4	0.0	0.0	22.679373030106866	0.0
32	5.306109454425826E-4	0.0	0.0	23.11818828198788	0.0
33	5.306109454425826E-4	0.0	0.0	23.540554594560177	0.0
34	5.306109454425826E-4	0.0	0.0	23.959206630514373	0.0
35	5.306109454425826E-4	0.0	0.0	24.456389086394076	0.0
36	5.306109454425826E-4	0.0	0.0	24.879286009911812	0.0
37	5.306109454425826E-4	0.0	0.0	25.32446859313814	0.0
38	5.306109454425826E-4	0.0	0.0	25.769120565419023	0.0
39	5.306109454425826E-4	0.0	0.0	26.22438475660876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	70	0.0	45.000004	4
ACGGGAG	45	3.8198777E-10	45.000004	5
GACGGGA	45	3.8198777E-10	45.000004	4
CGTAAGG	35	1.2047167E-7	45.000004	2
GCAATGG	35	1.2047167E-7	45.000004	2
GTGTTAT	20	7.018267E-4	45.0	20
AGCGTTG	20	7.018267E-4	45.0	1
GAGTGAT	20	7.018267E-4	45.0	9
AACCGAT	20	7.018267E-4	45.0	27
AGCCGGA	20	7.018267E-4	45.0	4
CAAACGG	20	7.018267E-4	45.0	2
CGAACGG	20	7.018267E-4	45.0	2
CTACTAG	20	7.018267E-4	45.0	25
TGCCAAC	20	7.018267E-4	45.0	41
AACCCGG	20	7.018267E-4	45.0	44
TGGGTAG	25	3.8783608E-5	45.0	6
GGCCGAT	20	7.018267E-4	45.0	8
GGTCCCG	20	7.018267E-4	45.0	18
ATAGCAG	25	3.8783608E-5	45.0	10
CACGCAG	20	7.018267E-4	45.0	1
>>END_MODULE