##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935634.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231725
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96195490344158	33.0	31.0	34.0	30.0	34.0
2	31.97109936346963	33.0	31.0	34.0	30.0	34.0
3	32.01781853490128	33.0	31.0	34.0	30.0	34.0
4	35.7168410831805	37.0	35.0	37.0	35.0	37.0
5	35.72760384075952	37.0	35.0	37.0	35.0	37.0
6	35.63312978746359	37.0	35.0	37.0	33.0	37.0
7	35.856489373179414	37.0	35.0	37.0	35.0	37.0
8	35.5683935699644	37.0	35.0	37.0	33.0	37.0
9	37.41658431330241	39.0	37.0	39.0	34.0	39.0
10	37.061970007552056	39.0	37.0	39.0	33.0	39.0
11	37.09013270039918	39.0	37.0	39.0	33.0	39.0
12	36.8742000215773	39.0	35.0	39.0	33.0	39.0
13	36.534163340166145	39.0	35.0	39.0	32.0	39.0
14	37.665301542777	40.0	36.0	41.0	33.0	41.0
15	37.61733520336606	40.0	36.0	41.0	32.0	41.0
16	37.740623583989645	39.0	36.0	41.0	33.0	41.0
17	37.5539238321286	39.0	35.0	41.0	32.0	41.0
18	37.55073902254828	39.0	36.0	41.0	33.0	41.0
19	37.445092242960406	39.0	36.0	41.0	32.0	41.0
20	37.25326140899774	39.0	35.0	41.0	32.0	41.0
21	37.56318480958032	39.0	35.0	41.0	33.0	41.0
22	37.616329701154385	39.0	35.0	41.0	33.0	41.0
23	37.558908188585605	39.0	35.0	41.0	33.0	41.0
24	37.463510626820586	39.0	35.0	41.0	32.0	41.0
25	37.47425612255907	39.0	35.0	41.0	33.0	41.0
26	37.32496277915633	39.0	35.0	41.0	32.0	41.0
27	37.29697270471464	39.0	35.0	41.0	33.0	41.0
28	37.232344373718846	39.0	35.0	41.0	32.0	41.0
29	37.121821124177366	39.0	35.0	41.0	32.0	41.0
30	37.12502751105837	39.0	35.0	41.0	32.0	41.0
31	37.0957427985759	39.0	35.0	41.0	32.0	41.0
32	36.871714316539	39.0	35.0	41.0	31.0	41.0
33	36.83753587226238	39.0	35.0	41.0	31.0	41.0
34	36.71127413960514	39.0	35.0	41.0	31.0	41.0
35	36.63864063005718	39.0	35.0	41.0	31.0	41.0
36	36.68493688639551	39.0	35.0	41.0	31.0	41.0
37	36.58468443197756	39.0	35.0	41.0	31.0	41.0
38	36.40626604811738	39.0	35.0	41.0	30.0	41.0
39	36.39971086417089	39.0	35.0	41.0	30.0	41.0
40	36.17158701046499	39.0	35.0	41.0	30.0	41.0
41	36.26632430682922	39.0	35.0	41.0	30.0	41.0
42	36.23956845398641	39.0	35.0	41.0	30.0	41.0
43	36.25670514618621	39.0	35.0	41.0	30.0	41.0
44	36.299212428525195	39.0	35.0	41.0	30.0	41.0
45	36.34497356780667	39.0	35.0	41.0	30.0	41.0
46	36.13084906678174	39.0	35.0	41.0	30.0	41.0
47	35.858172402632434	38.0	35.0	40.0	29.0	41.0
48	35.96283525730931	38.0	35.0	40.0	29.0	41.0
49	36.03516236918762	39.0	35.0	40.0	29.0	41.0
50	35.809463804078106	39.0	35.0	40.0	29.0	41.0
51	34.72618836983494	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	2.0
12	1.0
13	4.0
14	4.0
15	6.0
16	10.0
17	28.0
18	62.0
19	187.0
20	437.0
21	885.0
22	1307.0
23	1754.0
24	1799.0
25	1961.0
26	2024.0
27	2162.0
28	2203.0
29	2514.0
30	3360.0
31	4611.0
32	6527.0
33	8990.0
34	16035.0
35	23111.0
36	15572.0
37	21910.0
38	41360.0
39	72840.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.44805264861366	15.858452907541269	20.100981767180926	12.59251267666415
2	24.852303376847555	37.18890926745064	21.89664472974431	16.06214262595749
3	25.399935268097963	37.40554536627468	23.91239615924048	13.282123206386881
4	24.64602438234977	21.54320854461107	38.311360448807854	15.49940662423131
5	33.97216528212321	24.978746358830513	22.079188693494444	18.96989966555184
6	19.35483870967742	42.654871075628435	24.69392598985867	13.296364224835472
7	68.6378250080915	5.369295501132808	21.827597367569318	4.165282123206387
8	68.54245333908729	16.190743338008414	9.83148128169166	5.435322041212644
9	65.8008415147265	5.303268961052972	9.547524004747006	19.348365519473514
10	43.224943359585716	21.10734707088143	19.309957924263674	16.35775164526918
11	36.27101089653684	22.473190203905492	23.39669867299601	17.859100226561658
12	28.203689718416225	19.466177581184592	27.562412342215985	24.76772035818319
13	24.391412234329486	25.27953393030532	34.80418599633186	15.524867839033337
14	19.257740856618838	35.4113712374582	24.72888121695976	20.60200668896321
15	14.319991369079728	23.60340921350739	42.33682166360988	19.739777753803
16	17.21566512029345	24.40694789081886	30.667385910022656	27.710001078865034
17	17.87808825115978	26.303592620563165	31.073039162800736	24.745279965476318
18	19.059229690365736	24.66026540079836	30.921998058042938	25.358506850792967
19	19.96245549681735	27.576653360664583	27.029884561441364	25.43100658107671
20	23.835149422807206	26.110691552486788	32.772035818319125	17.28212320638688
21	20.405221706764483	33.29204876469953	27.44330564246413	18.859423886071852
22	17.758118459380732	23.18092566619916	31.850253533282984	27.21070234113712
23	19.707843348797066	31.62412342215989	28.461754234545257	20.20627899449779
24	21.515589599741073	25.927716042723052	27.333261408997732	25.22343294853814
25	16.65897076275758	35.720789729204874	26.479663394109398	21.140576113928148
26	16.917466824900202	27.27543424317618	30.74247491638796	25.064624015535657
27	22.079188693494444	29.354191390657032	27.146401985111662	21.420217930736865
28	15.296148451828676	30.18060200668896	33.799546876685724	20.723702664796633
29	19.38159456252023	26.704067321178123	30.398964289567378	23.515373826734276
30	18.24447081670083	31.135613334771822	32.56014672564462	18.059769122882727
31	23.268961052972273	27.23702664796634	23.229690365735248	26.26432193332614
32	25.299385046930627	30.781745603624987	22.398964289567374	21.519905059877008
33	23.985327435537815	24.578703204229154	23.643974538785198	27.791994821447837
34	20.81044341352897	29.477182004531233	24.085446110691553	25.626928471248245
35	19.001834070557774	27.658215557233788	28.257632970115438	25.082317402092997
36	20.29388283525731	32.81648505771928	23.98575898155141	22.903873125472003
37	16.790160750890063	26.041644190311793	32.77635127845506	24.391843780343077
38	20.604595965044773	31.31384183838602	22.41234221598878	25.669219980580426
39	21.329161721868594	24.631783363901175	24.736649045204445	29.302405869025783
40	22.890063653037004	26.426151688423776	27.13086632862229	23.552918329916928
41	16.812601143596936	25.786168950264322	23.933110367892976	33.468119538245766
42	18.73427554212968	27.974970331211562	29.13884992987377	24.151904196784983
43	22.468874743769554	24.391412234329486	23.866220735785955	29.273492286115005
44	20.895457978206927	24.18211241773654	29.701154385586364	25.22127521847017
45	17.980364656381486	26.662638903873127	26.203905491423026	29.15309094832236
46	26.040781098284604	27.90117596288704	24.18901715395404	21.869025784874314
47	17.129787463588304	25.27262919408782	35.44373718847772	22.153846153846153
48	21.55744956305966	26.308771172726292	25.416334016614524	26.717445247599525
49	18.872370266479663	21.66490452044449	33.97734383428633	25.485381378789512
50	21.155680224403927	23.464019851116625	29.221275218470172	26.159024706009276
51	19.174452475995253	24.309418491746683	25.567375121372315	30.948753910885745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	461.0
1	419.5
2	378.0
3	607.0
4	836.0
5	623.5
6	411.0
7	416.0
8	421.0
9	443.0
10	465.0
11	457.0
12	449.0
13	450.0
14	451.0
15	432.0
16	413.0
17	430.0
18	447.0
19	434.5
20	422.0
21	492.0
22	562.0
23	575.5
24	589.0
25	879.5
26	1416.5
27	1663.0
28	1917.0
29	2171.0
30	2626.0
31	3081.0
32	3574.0
33	4067.0
34	4822.0
35	5577.0
36	6269.5
37	6962.0
38	7588.5
39	8215.0
40	8984.0
41	9753.0
42	11708.0
43	13663.0
44	15436.0
45	17209.0
46	32508.5
47	47808.0
48	36126.5
49	24445.0
50	23178.5
51	21912.0
52	18924.5
53	15937.0
54	13595.0
55	11253.0
56	10261.0
57	9269.0
58	8209.0
59	7149.0
60	5882.5
61	4616.0
62	4352.0
63	4088.0
64	3363.0
65	2638.0
66	1894.5
67	1151.0
68	865.0
69	579.0
70	518.0
71	457.0
72	357.5
73	258.0
74	202.5
75	120.0
76	93.0
77	71.0
78	49.0
79	36.0
80	23.0
81	17.5
82	12.0
83	6.5
84	1.0
85	1.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	231725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.68302945301543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46196007734468	22.399395835580968
2	9.266824651440036	5.50134858129248
3	3.2362647747263136	2.8818642787787248
4	1.6995478533940074	2.0179091595641387
5	1.0889318581626273	1.6161398209084041
6	0.8301469839931378	1.4784766425720142
7	0.6513237282468052	1.3533282986298414
8	0.5684544146082607	1.349875930521092
9	0.47976971053894135	1.2816916603732873
>10	6.471075701844933	41.02578487431222
>50	0.18318479856941394	3.3099579242636747
>100	0.0494308186615879	2.933218254396375
>500	0.0	0.0
>1k	0.00872308564616257	3.542561225590678
>5k	0.004361542823081285	9.308447513216096
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGC	8207	3.5416981335634907	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCG	7223	3.1170568561872907	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTC	6140	2.6496925234653146	No Hit
GCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	1945	0.8393569964397454	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT	1437	0.6201316215341461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATACGCACT	1364	0.588628762541806	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTA	1265	0.5459057071960298	No Hit
GAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT	1126	0.4859208113065056	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTC	1072	0.4626173265724458	No Hit
CCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGC	469	0.202395080375445	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.18513323983169705	No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT	406	0.17520768151904198	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCATACGCACTCGTATG	372	0.1605351170568562	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCATACGCAC	340	0.1467256446218578	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATACGCACTCGTAT	311	0.13421081022764053	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT	294	0.12687452799654764	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATACGCACTCGT	291	0.12557988995576655	No Hit
GGGGAAGGGAAGGAAAGGAAAGGAAGGGAAAGAGAAGAGAAGAGAAGAGAA	254	0.10961268745279965	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	249	0.10745495738483114	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18729096989966554	0.0
2	0.0	0.0	0.0	1.4663933541913907	0.0
3	0.0	0.0	0.0	1.8202610853382242	0.0
4	4.3154601359369945E-4	0.0	0.0	2.691552486783903	0.0
5	4.3154601359369945E-4	0.0	0.0	6.431330240586902	0.0
6	4.3154601359369945E-4	0.0	0.0	7.187830402416657	0.0
7	4.3154601359369945E-4	0.0	0.0	8.44319775596073	0.0
8	4.3154601359369945E-4	0.0	0.0	9.855647858452908	0.0
9	4.3154601359369945E-4	0.0	0.0	10.22332506203474	0.0
10	4.3154601359369945E-4	0.0	0.0	14.239292264537706	0.0
11	8.630920271873989E-4	0.0	0.0	15.246952206278994	0.0
12	8.630920271873989E-4	0.0	0.0	19.815729852195492	0.0
13	8.630920271873989E-4	0.0	0.0	20.36854029560902	0.0
14	8.630920271873989E-4	0.0	0.0	20.63221490991477	0.0
15	8.630920271873989E-4	0.0	0.0	21.463372532096233	0.0
16	8.630920271873989E-4	0.0	0.0	22.097745172078973	0.0
17	8.630920271873989E-4	0.0	0.0	22.786061063760922	0.0
18	8.630920271873989E-4	0.0	0.0	23.528320207142087	0.0
19	8.630920271873989E-4	0.0	0.0	24.626173265724457	4.3154601359369945E-4
20	8.630920271873989E-4	0.0	0.0	25.187183083396267	4.3154601359369945E-4
21	8.630920271873989E-4	0.0	0.0	25.709353759844642	4.3154601359369945E-4
22	8.630920271873989E-4	0.0	0.0	26.29237242420973	4.3154601359369945E-4
23	8.630920271873989E-4	0.0	0.0	26.829215665120294	4.3154601359369945E-4
24	8.630920271873989E-4	0.0	0.0	27.23918437803431	4.3154601359369945E-4
25	8.630920271873989E-4	0.0	0.0	27.586578918977235	4.3154601359369945E-4
26	8.630920271873989E-4	0.0	0.0	27.933973459920164	4.3154601359369945E-4
27	8.630920271873989E-4	0.0	0.0	28.414715719063544	4.3154601359369945E-4
28	8.630920271873989E-4	0.0	0.0	28.770741180278346	4.3154601359369945E-4
29	8.630920271873989E-4	0.0	0.0	29.157406408458304	4.3154601359369945E-4
30	8.630920271873989E-4	0.0	0.0	29.616139820908405	4.3154601359369945E-4
31	8.630920271873989E-4	0.0	0.0	30.03560254612148	4.3154601359369945E-4
32	8.630920271873989E-4	0.0	0.0	30.423562412342218	4.3154601359369945E-4
33	8.630920271873989E-4	0.0	0.0	30.787355701801705	4.3154601359369945E-4
34	8.630920271873989E-4	0.0	0.0	31.164526917682597	4.3154601359369945E-4
35	8.630920271873989E-4	0.0	0.0	31.548171323767395	4.3154601359369945E-4
36	8.630920271873989E-4	0.0	0.0	31.90462833099579	4.3154601359369945E-4
37	8.630920271873989E-4	0.0	0.0	32.26971625849606	4.3154601359369945E-4
38	8.630920271873989E-4	0.0	0.0	32.60502751105837	4.3154601359369945E-4
39	8.630920271873989E-4	0.0	0.0	32.9727047146402	4.3154601359369945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	35	1.2063174E-7	45.000004	13
CTATGCG	35	1.2063174E-7	45.000004	1
CGAACCC	35	1.2063174E-7	45.000004	34
GCGAACC	35	1.2063174E-7	45.000004	33
GGGTATA	30	2.1579763E-6	45.000004	7
GGCCGGT	30	2.1579763E-6	45.000004	9
AACGAGC	35	1.2063174E-7	45.000004	15
CATCGGG	30	2.1579763E-6	45.000004	3
GACGCTT	30	2.1579763E-6	45.000004	9
ACCCGGT	30	2.1579763E-6	45.000004	13
TTACCCG	30	2.1579763E-6	45.000004	11
CGATCAC	30	2.1579763E-6	45.000004	34
CGATACC	30	2.1579763E-6	45.000004	34
TTGGGCG	35	1.2063174E-7	45.000004	5
GTTGAAC	20	7.0216187E-4	45.0	43
CGGGTTA	20	7.0216187E-4	45.0	6
GTTTGCG	20	7.0216187E-4	45.0	1
CGGAAAC	20	7.0216187E-4	45.0	37
CGAACAC	20	7.0216187E-4	45.0	34
GTTCTCG	20	7.0216187E-4	45.0	23
>>END_MODULE