SRR2935632.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935632.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629120
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	4622	0.734677009155646	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	4557	0.7243451169888098	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	3948	0.6275432349949136	RNA PCR Primer, Index 36 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2082	0.3309384537131231	RNA PCR Primer, Index 15 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1729	0.2748283316378433	RNA PCR Primer, Index 36 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1315	0.20902212614445576	No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	929	0.14766658189216683	RNA PCR Primer, Index 36 (96% over 26bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT	923	0.14671286876907427	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	874	0.13892421159715157	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCT	760	0.12080366225839267	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT	717	0.11396871820956256	RNA PCR Primer, Index 15 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC	636	0.10109359104781282	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	20	7.0308486E-4	45.0	22
GCGACGT	25	3.8887858E-5	45.0	17
CGAGTGT	20	7.0308486E-4	45.0	25
CGACGTT	25	3.8887858E-5	45.0	27
CGACGTC	20	7.0308486E-4	45.0	18
CGACGGC	20	7.0308486E-4	45.0	42
TCGATTA	20	7.0308486E-4	45.0	41
ATCCGGT	20	7.0308486E-4	45.0	11
TTCCCGC	25	3.8887858E-5	45.0	25
TACGCGG	20	7.0308486E-4	45.0	2
TCTACGG	25	3.8887858E-5	45.0	2
AACGCGC	20	7.0308486E-4	45.0	30
ACGTCGC	20	7.0308486E-4	45.0	20
ACGTAAC	25	3.8887858E-5	45.0	11
TCGACGT	20	7.0308486E-4	45.0	26
ATACCGG	20	7.0308486E-4	45.0	2
TACGGGC	80	0.0	42.1875	4
CGAGATA	70	0.0	41.785713	19
ATGGGAC	255	0.0	41.47059	5
GCTACGA	115	0.0	41.086956	10