##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935632.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629120
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.118400305188196	33.0	31.0	34.0	30.0	34.0
2	32.12988777975585	33.0	31.0	34.0	30.0	34.0
3	32.151889941505594	33.0	31.0	34.0	30.0	34.0
4	35.80668393947101	37.0	35.0	37.0	35.0	37.0
5	35.86845434893184	37.0	35.0	37.0	35.0	37.0
6	35.80639464649033	37.0	35.0	37.0	35.0	37.0
7	36.04890481943031	37.0	35.0	37.0	35.0	37.0
8	35.818851729399796	37.0	35.0	37.0	35.0	37.0
9	37.63457051119023	39.0	37.0	39.0	35.0	39.0
10	37.31179266276704	39.0	37.0	39.0	34.0	39.0
11	37.33051246185148	39.0	37.0	39.0	34.0	39.0
12	37.270938771617494	39.0	37.0	39.0	34.0	39.0
13	37.22544983468973	39.0	37.0	39.0	34.0	39.0
14	38.40265768056968	40.0	38.0	41.0	34.0	41.0
15	38.48109263733469	40.0	38.0	41.0	34.0	41.0
16	38.28794029755849	40.0	37.0	41.0	34.0	41.0
17	38.325241607324514	40.0	37.0	41.0	34.0	41.0
18	38.258461024923704	40.0	37.0	41.0	34.0	41.0
19	38.14213822482197	40.0	37.0	41.0	34.0	41.0
20	38.25865812563581	40.0	37.0	41.0	34.0	41.0
21	38.22008996693795	40.0	37.0	41.0	34.0	41.0
22	38.306490017802645	40.0	37.0	41.0	34.0	41.0
23	38.32109454476094	40.0	37.0	41.0	34.0	41.0
24	38.262512716174975	40.0	37.0	41.0	34.0	41.0
25	38.01960357324517	40.0	37.0	41.0	34.0	41.0
26	38.0984375	40.0	37.0	41.0	34.0	41.0
27	38.09272475839268	40.0	37.0	41.0	34.0	41.0
28	37.95301373346897	40.0	37.0	41.0	34.0	41.0
29	37.94461152085453	40.0	37.0	41.0	34.0	41.0
30	37.80062945066124	40.0	36.0	41.0	33.0	41.0
31	37.75952441505595	40.0	36.0	41.0	33.0	41.0
32	37.72400019074262	40.0	36.0	41.0	33.0	41.0
33	37.67085611648016	40.0	36.0	41.0	33.0	41.0
34	37.569652848423196	40.0	36.0	41.0	33.0	41.0
35	37.51474599440488	40.0	36.0	41.0	33.0	41.0
36	37.39728350712106	40.0	36.0	41.0	33.0	41.0
37	37.391343463886066	40.0	36.0	41.0	33.0	41.0
38	37.22693444811801	40.0	36.0	41.0	32.0	41.0
39	37.16699516785351	40.0	35.0	41.0	32.0	41.0
40	37.03457369023398	40.0	35.0	41.0	31.0	41.0
41	36.886754514242114	39.0	35.0	41.0	31.0	41.0
42	36.9619754577823	40.0	35.0	41.0	31.0	41.0
43	36.90151481434385	39.0	35.0	41.0	31.0	41.0
44	36.79160732451678	39.0	35.0	41.0	31.0	41.0
45	36.77569780010173	39.0	35.0	41.0	31.0	41.0
46	36.63250095371313	39.0	35.0	41.0	31.0	41.0
47	36.50856116480163	39.0	35.0	41.0	31.0	41.0
48	36.41364127670397	39.0	35.0	41.0	31.0	41.0
49	36.36134123855544	39.0	35.0	41.0	30.0	41.0
50	36.207397634791455	39.0	35.0	41.0	30.0	41.0
51	35.00693349440488	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	14.0
10	24.0
11	7.0
12	15.0
13	18.0
14	18.0
15	23.0
16	47.0
17	86.0
18	160.0
19	299.0
20	599.0
21	964.0
22	1454.0
23	1985.0
24	2655.0
25	3462.0
26	4517.0
27	5317.0
28	6043.0
29	7028.0
30	8597.0
31	10811.0
32	14199.0
33	19410.0
34	35362.0
35	47037.0
36	41529.0
37	64111.0
38	121590.0
39	231571.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.33027085452696	19.350998219735505	20.764401068158698	13.55432985757884
2	29.354018311291963	28.260427263479144	24.566060528992878	17.819493896236015
3	31.45234613428281	27.197831892166835	25.4385490844354	15.911272889114954
4	29.114000508647	24.637906917599185	28.055378942014244	18.192713631739572
5	27.47933621566633	29.740272126144458	23.609168362156662	19.17122329603255
6	24.79161368260427	36.842255849440484	24.419188708036625	13.946941759918616
7	76.61002670396745	6.777085452695829	11.915691759918616	4.697196083418108
8	76.98467700915565	9.086501780264497	9.047240590030519	4.881580620549339
9	70.78808494404883	8.898938199389624	10.902212614445576	9.41076424211597
10	38.63539547304171	27.44086978636826	18.75476856561546	15.16896617497457
11	30.544570193285857	26.556618769074262	24.533634282807732	18.365176754832145
12	27.500953713123096	23.158697863682605	29.06997075279756	20.270377670396744
13	25.404215412004067	24.621693794506612	30.216016022380465	19.75807477110885
14	20.53757629704985	29.86123474059003	27.44532044760936	22.15586851475076
15	19.092541963377414	26.64229399796541	34.002733977619535	20.26243006103764
16	21.117751780264495	25.757407171922686	31.13173957273652	21.993101475076298
17	21.363809766022378	26.29673194303154	28.77193540183113	23.567522889114954
18	21.30213631739573	26.94859486266531	29.04326678535097	22.706002034587996
19	23.680855798575788	27.739223041709053	26.662639877924722	21.917281281790437
20	25.244627416073246	26.282108341810783	28.98493133265514	19.488332909460834
21	24.357833163784335	27.980035605289928	28.08621566632757	19.57591556459817
22	22.031885808748726	25.79666836215666	27.93393947100712	24.237506358087487
23	21.529278992878943	28.264401068158694	28.26694430315361	21.93937563580875
24	22.63431459816887	25.31329476093591	28.86682985757884	23.18556078331638
25	21.371280518819937	28.94344481180061	26.95972151576806	22.725553153611393
26	21.153198118006102	27.86463631739573	27.79676373346897	23.185401831129195
27	23.369786368260428	28.410001271617496	27.052072736520856	21.16813962360122
28	20.11078967446592	28.68387589013225	29.20905391658189	21.996280518819937
29	22.98321464903357	26.841461088504577	27.526227110885042	22.649097151576804
30	22.848582146490333	27.87083545269583	27.90262589013225	21.377956510681585
31	23.081606052899286	29.057254577822988	24.983627924720246	22.877511444557477
32	23.794347660223806	29.14134028484232	25.683971261444555	21.38034079348932
33	22.59998092573754	28.996534842319434	25.234613428280774	23.16887080366226
34	22.777371566632755	28.221642929806713	26.607483468972532	22.393502034587996
35	22.523683875890132	26.44789547304171	26.87341047812818	24.15501017293998
36	22.926150813835196	29.579412512716175	25.789197609359103	21.705239064089522
37	22.206256358087487	29.395186927772126	27.29622329603255	21.102333418107833
38	20.604336215666326	30.232388097660223	25.711787894201425	23.451487792472026
39	21.936991353001016	27.90119532044761	26.067840793489317	24.093972533062054
40	22.045396744659207	26.765164038657176	29.769996185147505	21.419443031536115
41	20.019233214649034	27.707750508647	27.548957273652086	24.72405900305188
42	21.22361393692777	26.93015640895219	29.054234486266534	22.79199516785351
43	22.72444048830112	26.077060020345883	26.553280773143438	24.645218718209563
44	21.234422685656153	27.346452187182095	27.603001017293998	23.81612410986775
45	21.20581129196338	26.436450915564595	26.319144201424212	26.038593591047814
46	22.518597405900305	28.183494404883014	26.127288911495423	23.17061927772126
47	20.850553153611393	26.949548575788402	30.095212360122076	22.10468591047813
48	21.231561546286876	26.287194811800614	28.282680569684636	24.198563072227874
49	20.037671668362155	25.719417599186166	30.4749491353001	23.767961597151576
50	20.305982960325533	26.085007629704986	28.68180951169888	24.9271998982706
51	20.33141531027467	25.717033316378433	27.30130976602238	26.650241607324514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	673.0
1	1044.5
2	1416.0
3	2077.0
4	2738.0
5	1984.5
6	1231.0
7	1215.5
8	1200.0
9	1192.5
10	1185.0
11	1274.0
12	1363.0
13	1266.0
14	1169.0
15	1232.0
16	1295.0
17	1382.0
18	1469.0
19	1503.5
20	1538.0
21	1981.0
22	2424.0
23	2583.0
24	2742.0
25	3301.0
26	5181.0
27	6502.0
28	7665.0
29	8828.0
30	9928.5
31	11029.0
32	13458.0
33	15887.0
34	17761.0
35	19635.0
36	19781.5
37	19928.0
38	21780.5
39	23633.0
40	27406.5
41	31180.0
42	33929.5
43	36679.0
44	40335.5
45	43992.0
46	55498.0
47	67004.0
48	62683.0
49	58362.0
50	56583.5
51	54805.0
52	47748.0
53	40691.0
54	36789.5
55	32888.0
56	30956.0
57	29024.0
58	27369.5
59	25715.0
60	25312.0
61	24909.0
62	22607.5
63	20306.0
64	16580.5
65	12855.0
66	10315.5
67	7776.0
68	6587.5
69	5399.0
70	4411.5
71	3424.0
72	2642.0
73	1860.0
74	1572.5
75	1035.0
76	785.0
77	500.5
78	216.0
79	156.0
80	96.0
81	82.5
82	69.0
83	43.0
84	17.0
85	15.0
86	13.0
87	11.5
88	10.0
89	8.5
90	7.0
91	5.0
92	3.0
93	4.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.436832303879644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.98211738881028	21.038170656715362
2	9.610363628668706	5.465765977755125
3	3.3690314043965155	2.874137432199836
4	1.7151129240865037	1.9508951441785827
5	1.045672920952811	1.486781274892154
6	0.7769357145760718	1.3256154375776792
7	0.6566328322368834	1.3070790414879256
8	0.5392996735984958	1.2268779503725964
9	0.49852787091570794	1.2758898117636133
>10	7.425986564416146	49.55339376834183
>50	0.31468879408379175	5.488727693003671
>100	0.05609425919497142	2.881785419564759
>500	0.006170368511446856	1.2132657976978611
>1k	0.0033656555516982847	2.9116145944489955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	4622	0.734677009155646	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	4557	0.7243451169888098	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	3948	0.6275432349949136	RNA PCR Primer, Index 36 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2082	0.3309384537131231	RNA PCR Primer, Index 15 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1729	0.2748283316378433	RNA PCR Primer, Index 36 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1315	0.20902212614445576	No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	929	0.14766658189216683	RNA PCR Primer, Index 36 (96% over 26bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCT	923	0.14671286876907427	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	874	0.13892421159715157	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACAATCT	760	0.12080366225839267	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCT	717	0.11396871820956256	RNA PCR Primer, Index 15 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTC	636	0.10109359104781282	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.358087487283825E-4	0.0	0.0	0.15752161749745677	0.0
2	6.358087487283825E-4	0.0	0.0	0.9421096134282808	0.0
3	6.358087487283825E-4	0.0	0.0	1.2512716174974567	0.0
4	6.358087487283825E-4	0.0	0.0	1.7109613428280772	0.0
5	6.358087487283825E-4	0.0	0.0	3.2448499491353	0.0
6	6.358087487283825E-4	0.0	0.0	3.9691950661241098	0.0
7	6.358087487283825E-4	0.0	0.0	4.669697355035606	0.0
8	6.358087487283825E-4	0.0	0.0	5.576360630722279	0.0
9	6.358087487283825E-4	0.0	0.0	5.889814343845371	0.0
10	6.358087487283825E-4	0.0	0.0	7.365367497456765	0.0
11	6.358087487283825E-4	0.0	0.0	8.36088504577823	0.0
12	6.358087487283825E-4	0.0	0.0	9.92115971515768	0.0
13	6.358087487283825E-4	0.0	0.0	10.315202187182095	0.0
14	6.358087487283825E-4	0.0	0.0	10.493387589013224	0.0
15	7.947609359104782E-4	0.0	0.0	10.850394201424212	0.0
16	7.947609359104782E-4	0.0	0.0	11.3911495422177	0.0
17	7.947609359104782E-4	0.0	0.0	12.047781027466938	0.0
18	7.947609359104782E-4	0.0	0.0	12.781186419125127	0.0
19	7.947609359104782E-4	0.0	0.0	13.31908062054934	0.0
20	9.537131230925738E-4	0.0	0.0	13.752066378433367	0.0
21	9.537131230925738E-4	0.0	0.0	14.27422431332655	0.0
22	9.537131230925738E-4	0.0	0.0	14.846611139369278	0.0
23	9.537131230925738E-4	0.0	0.0	15.40199008138352	0.0
24	9.537131230925738E-4	0.0	0.0	15.83656536113937	0.0
25	9.537131230925738E-4	0.0	0.0	16.212487283825027	0.0
26	9.537131230925738E-4	0.0	0.0	16.580779501525942	0.0
27	0.0011126653102746693	0.0	0.0	16.991353001017295	0.0
28	0.001271617497456765	0.0	0.0	17.38030900305188	0.0
29	0.001271617497456765	0.0	0.0	17.790882502543234	0.0
30	0.001271617497456765	0.0	0.0	18.257566124109868	0.0
31	0.001271617497456765	0.0	0.0	18.684193794506612	0.0
32	0.001271617497456765	0.0	0.0	19.08983977619532	0.0
33	0.001271617497456765	0.0	0.0	19.4948499491353	0.0
34	0.001271617497456765	0.0	0.0	19.89620422177009	0.0
35	0.001271617497456765	0.0	0.0	20.30550610376399	0.0
36	0.001271617497456765	0.0	0.0	20.713377416073246	0.0
37	0.001271617497456765	0.0	0.0	21.133805951169887	0.0
38	0.001271617497456765	0.0	0.0	21.51560910478128	0.0
39	0.001271617497456765	0.0	0.0	21.92665946083418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	20	7.0308486E-4	45.0	22
GCGACGT	25	3.8887858E-5	45.0	17
CGAGTGT	20	7.0308486E-4	45.0	25
CGACGTT	25	3.8887858E-5	45.0	27
CGACGTC	20	7.0308486E-4	45.0	18
CGACGGC	20	7.0308486E-4	45.0	42
TCGATTA	20	7.0308486E-4	45.0	41
ATCCGGT	20	7.0308486E-4	45.0	11
TTCCCGC	25	3.8887858E-5	45.0	25
TACGCGG	20	7.0308486E-4	45.0	2
TCTACGG	25	3.8887858E-5	45.0	2
AACGCGC	20	7.0308486E-4	45.0	30
ACGTCGC	20	7.0308486E-4	45.0	20
ACGTAAC	25	3.8887858E-5	45.0	11
TCGACGT	20	7.0308486E-4	45.0	26
ATACCGG	20	7.0308486E-4	45.0	2
TACGGGC	80	0.0	42.1875	4
CGAGATA	70	0.0	41.785713	19
ATGGGAC	255	0.0	41.47059	5
GCTACGA	115	0.0	41.086956	10
>>END_MODULE