##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935631.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	522283
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883365914647804	33.0	31.0	34.0	30.0	34.0
2	31.907705209627732	33.0	31.0	34.0	30.0	34.0
3	31.955108628846812	33.0	31.0	34.0	30.0	34.0
4	35.66530788863509	37.0	35.0	37.0	35.0	37.0
5	35.670496646454126	37.0	35.0	37.0	35.0	37.0
6	35.573403691102335	37.0	35.0	37.0	33.0	37.0
7	35.79299728308216	37.0	35.0	37.0	35.0	37.0
8	35.46090529463911	37.0	35.0	37.0	33.0	37.0
9	37.21137391031299	39.0	37.0	39.0	34.0	39.0
10	37.04875709146191	39.0	37.0	39.0	33.0	39.0
11	37.05041519635906	39.0	37.0	39.0	33.0	39.0
12	36.85895386217817	39.0	35.0	39.0	33.0	39.0
13	36.60573290725526	39.0	35.0	39.0	32.0	39.0
14	37.71928054330698	40.0	36.0	41.0	32.0	41.0
15	37.64168850986917	40.0	36.0	41.0	32.0	41.0
16	37.73661788723738	40.0	36.0	41.0	33.0	41.0
17	37.494940482458745	39.0	36.0	41.0	32.0	41.0
18	37.51706258867319	39.0	36.0	41.0	32.0	41.0
19	37.41528251924723	39.0	36.0	41.0	32.0	41.0
20	37.15025761895371	39.0	35.0	41.0	31.0	41.0
21	37.48153587231443	39.0	35.0	41.0	32.0	41.0
22	37.51700706322051	39.0	35.0	41.0	32.0	41.0
23	37.390297980213795	39.0	35.0	41.0	32.0	41.0
24	37.357279482579365	39.0	35.0	41.0	32.0	41.0
25	37.40233743009058	39.0	35.0	41.0	33.0	41.0
26	37.23792465004605	39.0	35.0	41.0	32.0	41.0
27	37.145842388130575	39.0	35.0	41.0	32.0	41.0
28	37.02147303281937	39.0	35.0	41.0	31.0	41.0
29	36.980533542160096	39.0	35.0	41.0	31.0	41.0
30	36.88269769454491	39.0	35.0	41.0	31.0	41.0
31	36.90930013038908	39.0	35.0	41.0	31.0	41.0
32	36.572465502419185	39.0	35.0	41.0	30.0	41.0
33	36.436414740667416	39.0	35.0	41.0	30.0	41.0
34	36.3352760859534	39.0	35.0	41.0	30.0	41.0
35	36.19515090477768	39.0	35.0	41.0	30.0	41.0
36	36.1112079083562	39.0	35.0	41.0	29.0	41.0
37	36.20536950274085	39.0	35.0	41.0	30.0	41.0
38	35.84100190892677	39.0	35.0	41.0	27.0	41.0
39	35.93284292232372	39.0	35.0	41.0	28.0	41.0
40	35.76436146686758	39.0	35.0	41.0	27.0	41.0
41	35.558103556883914	38.0	35.0	41.0	26.0	41.0
42	35.79155362131258	39.0	35.0	41.0	27.0	41.0
43	35.716569369479764	39.0	35.0	41.0	28.0	41.0
44	35.85910320649916	39.0	35.0	41.0	28.0	41.0
45	35.845930271519464	39.0	35.0	40.0	28.0	41.0
46	35.69413517192786	38.0	35.0	40.0	28.0	41.0
47	35.444109802540005	38.0	35.0	40.0	27.0	41.0
48	35.485566254310406	38.0	35.0	40.0	27.0	41.0
49	35.43271176737516	38.0	35.0	40.0	27.0	41.0
50	35.25787169025222	38.0	35.0	40.0	26.0	41.0
51	34.3634121730939	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	9.0
11	12.0
12	12.0
13	11.0
14	18.0
15	14.0
16	34.0
17	67.0
18	180.0
19	513.0
20	1246.0
21	2460.0
22	3685.0
23	4526.0
24	5133.0
25	5358.0
26	5636.0
27	5839.0
28	5952.0
29	6792.0
30	8742.0
31	11642.0
32	15632.0
33	20691.0
34	34679.0
35	45823.0
36	36656.0
37	52631.0
38	91156.0
39	156995.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.95960236117201	15.618735436535363	17.990246667036836	13.431415535255791
2	24.921737831788512	38.1634094925548	20.851339216478422	16.06351345917826
3	26.5449957207108	37.406540132456925	22.327550389348303	13.720913757483968
4	24.1290641280685	20.567776473674236	38.25473928885298	17.04842010940429
5	34.66339130318237	24.836152047836137	21.02423398808692	19.476222660894575
6	21.865157395511627	41.64983351937551	23.355154198011423	13.12985488710144
7	67.2150922009715	5.64693853715323	22.43649515684026	4.70147410503501
8	65.50299358776755	18.113742932471478	10.543709061945345	5.839554417815629
9	60.76073699507738	7.134063333480125	10.894093815038973	21.21110585640352
10	42.852055303350866	24.21062910337882	18.263278720540395	14.674036872729918
11	37.74352219007703	21.016766772037382	23.297905541631643	17.941805496253945
12	29.948705969752034	17.888386181438033	26.32231950877206	25.840588340037872
13	28.42635123103758	21.28367187903876	32.86647277433882	17.423504115584844
14	20.316763134162898	32.532745657048004	23.928789564278368	23.221701644510734
15	16.00435013201655	22.744565685653182	38.82952345758908	22.421560724741184
16	18.584943411905037	23.978762471686807	28.129577259838058	29.306716856570098
17	18.99161948598748	26.455963529350946	30.85051590804219	23.70190107661938
18	19.612930154724545	24.983390230966737	28.08783743679193	27.315842177516785
19	22.022734800864665	27.07612539561885	24.490553971697338	26.41058583181915
20	27.584279021143708	24.940310138373256	31.21640949446947	16.25900134601356
21	23.22610538730918	33.15156725376855	25.246848930560635	18.375478428361635
22	19.80631190369972	23.26746227619892	30.915806181706085	26.010419638395277
23	23.195279187720068	29.995423936831184	27.528370634311283	19.28092624113747
24	23.35381392846407	25.201471233028837	25.345645942908344	26.099068895598748
25	19.7643806135754	34.54755372087546	23.50162651282925	22.186439152719885
26	18.857783998330408	24.02624630707873	29.540115224887657	27.57585446970321
27	25.004068675411606	27.283101307145742	23.85603207456494	23.856797942877712
28	17.614588259621698	28.57167474338625	30.977841515040694	22.835895481951358
29	22.048582856420754	23.94946800872324	26.620816683675326	27.381132451180683
30	19.956230625925027	30.60428924548568	26.47836517749955	22.961114951089733
31	26.291110375026566	24.533442597212623	21.144475313192274	28.030971714568537
32	27.572790996452113	30.555848074702794	21.528558272047913	20.342802656797176
33	24.335465638360812	27.361794276283163	21.84467041814495	26.45806966721107
34	23.07312319183278	27.644208216618193	23.714537903780133	25.568130687768893
35	20.754456874912645	24.16754901078534	23.44763279677876	31.63036131752326
36	22.357802187702834	28.333681165192047	24.399032708320966	24.909483938784145
37	18.96117622055476	29.398812521181046	26.41058583181915	25.22942542644505
38	23.64101454575393	28.287154665191096	21.779380144481056	26.29245064457392
39	22.152740946957874	32.19691240189705	23.83075842024343	21.819588230901637
40	20.15095264444755	26.40752235856806	26.72574064252522	26.71578435445917
41	17.793992911888765	30.131365562348382	26.658727165157586	25.415914360605267
42	19.44367325760172	24.279365784450192	28.846238533515354	27.43072242443273
43	22.086493337903015	24.51563615894065	27.1427559388301	26.255114564326238
44	21.70681412184582	24.489405169228178	27.757556726908593	26.046223982017413
45	21.001449405781923	22.298830327619317	25.539985027274486	31.159735239324277
46	25.768213784480825	28.47766440799337	23.744598235056475	22.00952357246933
47	17.545085710237554	26.17546425979785	33.97889649864154	22.300553531323057
48	21.912641230903553	26.655663691906494	25.18136718981855	26.250327887371405
49	19.7898457349751	22.362014463423087	33.07842682989873	24.769712971703083
50	21.419422037477766	23.242380088955606	28.478047342149754	26.860150531416878
51	19.063994041544525	23.04765807043308	25.3695793276825	32.51876856033989
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	514.0
1	750.5
2	987.0
3	1789.5
4	2592.0
5	1841.0
6	1090.0
7	1087.0
8	1084.0
9	1138.0
10	1192.0
11	1171.0
12	1150.0
13	1106.0
14	1062.0
15	1035.5
16	1009.0
17	968.0
18	927.0
19	1032.0
20	1137.0
21	1279.0
22	1421.0
23	1717.5
24	2014.0
25	2166.5
26	2900.0
27	3481.0
28	4331.0
29	5181.0
30	5850.0
31	6519.0
32	7541.5
33	8564.0
34	9935.5
35	11307.0
36	11669.0
37	12031.0
38	13684.0
39	15337.0
40	17605.0
41	19873.0
42	21832.5
43	23792.0
44	25855.0
45	27918.0
46	61505.5
47	95093.0
48	69750.5
49	44408.0
50	44396.5
51	44385.0
52	38346.5
53	32308.0
54	30757.5
55	29207.0
56	27792.0
57	26377.0
58	25502.5
59	24628.0
60	24477.5
61	24327.0
62	21182.0
63	18037.0
64	15070.5
65	12104.0
66	9707.0
67	7310.0
68	5641.5
69	3973.0
70	3588.5
71	3204.0
72	2379.5
73	1555.0
74	1349.0
75	1009.0
76	875.0
77	688.0
78	501.0
79	348.0
80	195.0
81	130.0
82	65.0
83	50.0
84	35.0
85	25.0
86	15.0
87	20.5
88	26.0
89	15.5
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	522283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.07847443257428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53704916133113	19.43812530833306
2	9.481397978616736	4.94520789540836
3	3.3054395621919848	2.586024633331296
4	1.703537489343734	1.7770263544312936
5	1.124130331343988	1.4657802052417723
6	0.8341900693300338	1.3052642636958391
7	0.6036770226560152	1.102008306060722
8	0.5497522034765411	1.146935902609148
9	0.4864061558570634	1.141625744942865
>10	6.747499745197487	39.912238837574165
>50	0.561432129272308	9.066280349056974
>100	0.05224335672127637	2.304612504332005
>500	0.005886575405214239	1.1271685137757035
>1k	0.005150753479562459	3.620026219335827
>5k	0.0	0.0
>10k+	0.0022074657769553395	9.061674961870965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGC	17754	3.3993065062427843	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCG	15745	3.014649146152565	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTC	13724	2.627694181124027	No Hit
GCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	3805	0.7285322325252784	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCT	3214	0.6153751893130736	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGAAACTT	2944	0.5636790782008987	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTA	2648	0.5070048230556997	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTC	2507	0.48000796503045284	No Hit
GAACTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCT	2468	0.47254074898091647	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1279	0.24488639300915405	No Hit
CCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGC	954	0.182659592596351	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATG	808	0.15470539918013798	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	754	0.144366176957703	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTAT	732	0.14015390123745172	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTT	712	0.13632455967358692	No Hit
CTGTCTCTTATACACATCTGACGCCCGAAACTTCGTATGCCGTCTTCTGCT	684	0.1309634814841762	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCGAAACTTCGT	640	0.12253893004367364	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCGAAACT	590	0.11296557613401165	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.744012345797202E-4	0.0	0.0	0.13881363169009905	0.0
2	5.744012345797202E-4	0.0	0.0	1.2248148992021566	0.0
3	5.744012345797202E-4	0.0	0.0	1.4781258436518132	0.0
4	5.744012345797202E-4	0.0	0.0	2.260268858071199	0.0
5	5.744012345797202E-4	0.0	0.0	5.69959198365637	0.0
6	5.744012345797202E-4	0.0	0.0	6.309031693545453	0.0
7	7.658683127729603E-4	0.0	0.0	7.391012152415453	0.0
8	7.658683127729603E-4	0.0	0.0	8.492522253261162	0.0
9	7.658683127729603E-4	0.0	0.0	8.75176867713481	0.0
10	7.658683127729603E-4	0.0	0.0	12.41798029037897	0.0
11	7.658683127729603E-4	0.0	0.0	13.21812121014852	0.0
12	7.658683127729603E-4	0.0	0.0	17.465435405709165	0.0
13	7.658683127729603E-4	0.0	0.0	17.933380944813443	0.0
14	7.658683127729603E-4	0.0	0.0	18.204689794613266	0.0
15	7.658683127729603E-4	0.0	0.0	18.97725945512299	0.0
16	7.658683127729603E-4	0.0	0.0	19.49613523702667	0.0
17	7.658683127729603E-4	0.0	0.0	20.02803078024749	0.0
18	7.658683127729603E-4	0.0	0.0	20.573903420176418	0.0
19	7.658683127729603E-4	0.0	0.0	21.58791306628782	0.0
20	9.573353909662003E-4	0.0	0.0	22.055092737079324	0.0
21	9.573353909662003E-4	0.0	0.0	22.460045607458024	0.0
22	9.573353909662003E-4	0.0	0.0	23.03885058483619	0.0
23	0.0011488024691594404	0.0	0.0	23.457972019001193	0.0
24	0.0013402695473526804	0.0	0.0	23.81582398814436	0.0
25	0.0013402695473526804	0.0	0.0	24.10666247991989	0.0
26	0.0013402695473526804	0.0	0.0	24.39788390585181	0.0
27	0.0013402695473526804	0.0	0.0	24.813558932609332	0.0
28	0.0017232037037391607	0.0	0.0	25.128905210393597	0.0
29	0.0017232037037391607	0.0	0.0	25.4319975951735	0.0
30	0.0017232037037391607	0.0	0.0	25.791764235098597	0.0
31	0.0017232037037391607	0.0	0.0	26.12242787913832	0.0
32	0.0017232037037391607	0.0	0.0	26.46630275157338	0.0
33	0.0019146707819324006	0.0	0.0	26.77935142441933	0.0
34	0.0019146707819324006	0.0	0.0	27.110206535537248	0.0
35	0.0019146707819324006	0.0	0.0	27.436274969700335	0.0
36	0.0019146707819324006	0.0	0.0	27.772682626085857	0.0
37	0.0019146707819324006	0.0	0.0	28.10736707876764	0.0
38	0.0019146707819324006	0.0	0.0	28.441668597293038	0.0
39	0.0019146707819324006	0.0	0.0	28.81062565697141	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.0297474E-4	45.0	1
TATCATA	20	7.0297474E-4	45.0	15
CTATGCG	20	7.0297474E-4	45.0	1
CAAACGG	35	1.2102282E-7	45.0	2
GACCGTA	20	7.0297474E-4	45.0	35
TCGGGTA	35	1.2102282E-7	45.0	27
GCCGGTT	20	7.0297474E-4	45.0	10
ACGGGTC	20	7.0297474E-4	45.0	5
ACGGGTA	20	7.0297474E-4	45.0	5
TATTGCG	20	7.0297474E-4	45.0	1
TTCCACG	20	7.0297474E-4	45.0	14
TGCGACG	25	3.8878723E-5	45.0	1
CATCGTT	20	7.0297474E-4	45.0	36
CGTAAGG	35	1.2102282E-7	45.0	2
AGTTACG	20	7.0297474E-4	45.0	1
CAATGTC	20	7.0297474E-4	45.0	10
CGAGAAT	20	7.0297474E-4	45.0	39
CGGTCTA	20	7.0297474E-4	45.0	31
CGATCGC	20	7.0297474E-4	45.0	34
TTCGAAT	45	3.8380676E-10	45.0	43
>>END_MODULE