FastQCFastQC Report
Sat 14 Jan 2017
SRR2935630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672860
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC129241.9207561751330142No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG125351.8629432571411586No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC113651.68905864518622No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC26350.39161192521475496TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT23040.3424189281574176No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT22990.34167583152513153No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT22640.3364741550991291No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.2938204084059091No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA17440.2591921053413786No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC15840.2354130131082246No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT12920.19201616978271854No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7490.11131587551645215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGT207.0312E-445.044
TATAGCG302.1641554E-644.9999961
ACACTCG1050.042.8571440
CCGATGA13550.041.8450218
CGATGAA14050.041.15658619
GATGAAT14900.041.07382620
TACGGCT13850.040.9386257
ACGGCTG13850.040.9386258
GCGAACC556.002665E-1140.90909233
CCACCGA18050.040.76177215
CGTTTTT10750.040.1860471
AACGGGA2250.040.04
CACCGAT15550.039.79099716
TACGAAA850.039.70588320
ATGAATG15550.039.21221521
CTCGAAT1900.039.07894543
TGATACC14350.039.041814
ACATGCG356.2444287E-638.5714261
AGTTACG356.2444287E-638.5714261
ACCGATG14800.038.15878317