##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935630.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672860
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05028980768659	33.0	31.0	34.0	30.0	34.0
2	32.08459560681271	33.0	31.0	34.0	30.0	34.0
3	32.1123710727343	33.0	31.0	34.0	30.0	34.0
4	35.76531224920489	37.0	35.0	37.0	35.0	37.0
5	35.7811060250275	37.0	35.0	37.0	35.0	37.0
6	35.700726748506376	37.0	35.0	37.0	35.0	37.0
7	35.9247882174598	37.0	35.0	37.0	35.0	37.0
8	35.67691793240793	37.0	35.0	37.0	35.0	37.0
9	37.468055761971286	39.0	37.0	39.0	35.0	39.0
10	37.21234134886901	39.0	37.0	39.0	34.0	39.0
11	37.198971554260915	39.0	37.0	39.0	34.0	39.0
12	37.01839610022888	39.0	37.0	39.0	33.0	39.0
13	36.838670748744164	39.0	35.0	39.0	33.0	39.0
14	37.89735011740927	40.0	37.0	41.0	33.0	41.0
15	37.93685313438159	40.0	37.0	41.0	33.0	41.0
16	37.95909847516571	40.0	36.0	41.0	33.0	41.0
17	37.84723419433463	40.0	36.0	41.0	33.0	41.0
18	37.83316588889219	40.0	36.0	41.0	33.0	41.0
19	37.71074369110959	40.0	36.0	41.0	33.0	41.0
20	37.56331034687751	40.0	36.0	41.0	32.0	41.0
21	37.7409684035312	40.0	36.0	41.0	33.0	41.0
22	37.82565466813304	40.0	36.0	41.0	33.0	41.0
23	37.77614511191035	40.0	36.0	41.0	33.0	41.0
24	37.76701691287935	40.0	36.0	41.0	33.0	41.0
25	37.67137443153108	40.0	36.0	41.0	33.0	41.0
26	37.592661177659544	40.0	35.0	41.0	33.0	41.0
27	37.51906488719793	40.0	35.0	41.0	33.0	41.0
28	37.38401450524626	40.0	35.0	41.0	32.0	41.0
29	37.33150729720893	40.0	35.0	41.0	32.0	41.0
30	37.18225336622774	40.0	35.0	41.0	32.0	41.0
31	37.158468329221535	40.0	35.0	41.0	32.0	41.0
32	36.97572154682995	40.0	35.0	41.0	31.0	41.0
33	36.899249472401394	40.0	35.0	41.0	31.0	41.0
34	36.714059388282855	40.0	35.0	41.0	31.0	41.0
35	36.642843979431085	40.0	35.0	41.0	31.0	41.0
36	36.46667360223523	39.0	35.0	41.0	30.0	41.0
37	36.449112742621054	39.0	35.0	41.0	30.0	41.0
38	36.23225633861427	39.0	35.0	41.0	30.0	41.0
39	36.20710251761139	39.0	35.0	41.0	30.0	41.0
40	36.082626400737155	39.0	35.0	41.0	29.0	41.0
41	35.94869363612044	39.0	35.0	41.0	28.0	41.0
42	35.98159201022501	39.0	35.0	41.0	29.0	41.0
43	35.98970513925631	39.0	35.0	41.0	29.0	41.0
44	36.00180572481646	39.0	35.0	41.0	29.0	41.0
45	36.01780310911631	39.0	35.0	41.0	29.0	41.0
46	35.908856225663584	39.0	35.0	41.0	29.0	41.0
47	35.683559730107305	38.0	35.0	41.0	28.0	41.0
48	35.620769550872396	38.0	35.0	40.0	28.0	41.0
49	35.62744255863032	38.0	35.0	40.0	28.0	41.0
50	35.48341111078084	38.0	35.0	40.0	28.0	41.0
51	34.369234313230095	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	17.0
11	14.0
12	18.0
13	19.0
14	18.0
15	29.0
16	60.0
17	112.0
18	284.0
19	675.0
20	1707.0
21	2822.0
22	3757.0
23	4589.0
24	4972.0
25	5746.0
26	6591.0
27	7055.0
28	7725.0
29	8562.0
30	10260.0
31	13450.0
32	18082.0
33	25097.0
34	44532.0
35	52307.0
36	45986.0
37	67263.0
38	117305.0
39	223648.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.113188479029816	17.22661474898196	20.53369200130785	13.12650477068038
2	28.381535534880957	32.846060101655624	22.668460006539252	16.103944356924174
3	29.29777368248967	31.648782807716312	24.177094789406414	14.8763487203876
4	26.155961121184202	22.505721844068603	33.91121481437446	17.42710222037274
5	31.442350563267247	25.96557976399251	23.52956038403234	19.062509288707904
6	22.895401718039412	39.342062241773924	23.996670927087358	13.765865113099307
7	72.8332788395803	5.696281544452041	17.17058526290759	4.299854353060072
8	73.49195969443866	12.96599589810659	9.128347650328449	4.413696757126297
9	68.34675861248996	6.736319590999614	9.953333531492435	14.963588265017982
10	43.414380406028	22.95752459649853	18.35478405611866	15.273310941354815
11	34.72401391076896	23.495377938947183	23.56002734595607	18.220580804327795
12	27.66310971078679	20.298427607526083	27.98799155842226	24.05047112326487
13	27.662663852807416	21.488422554468983	32.6509229260173	18.1979906667063
14	21.181077787355466	29.5456707190203	28.420771037065663	20.85248045655857
15	18.569241744196415	23.24421127723449	36.067235383289244	22.11931159527985
16	22.828225782480754	24.361382754213356	27.071010314181255	25.739381149124636
17	23.34408346461374	26.070356389144845	28.874803079392443	21.710757066848974
18	23.209731593496418	24.37460987426805	28.313319264037094	24.102339268198435
19	23.594209791041227	25.579615373183128	26.12594001723984	24.7002348185358
20	26.07273429836816	26.134114080194987	29.552209969384418	18.240941652052435
21	24.223909877240438	29.887792408524806	27.772642154385757	18.115655559849003
22	22.44255863032429	22.36081800077282	32.14858960259192	23.048033766310972
23	24.007965995898108	26.867401836934874	29.228368457034154	19.896263710132867
24	22.902238207056445	25.995006390631037	26.783580536813005	24.31917486549951
25	22.06432244449068	31.150462206105285	25.604137562048567	21.181077787355466
26	21.230716642392178	24.603632256338614	28.924887792408526	25.240763308860686
27	24.900127812620752	26.036173944059687	26.56258359837113	22.501114644948427
28	19.85034033825759	27.02642451624409	30.0587046339506	23.06453051154772
29	25.135094967749605	24.48830365900782	28.525101804238624	21.851499569003956
30	24.50197663704188	27.41001099783016	28.452872811580416	19.635139553547543
31	25.57084683292215	23.5699848408287	26.73958921618167	24.119579110067473
32	27.358885949528876	28.688434444015098	24.810956216746426	19.141723389709597
33	25.277026424516247	25.69152572600541	24.941295366049403	24.090152483428945
34	27.438397289183484	24.920637279671848	26.16874238325952	21.472223047885148
35	24.98335463543679	27.483577564426476	25.061677020479745	22.471390779656986
36	25.444371786107066	26.905745623160836	26.107659840085606	21.542222750646495
37	24.38293255654965	27.682430223226227	27.528609220343014	20.406027999881104
38	21.662158547097466	28.167820943435483	26.444133995184732	23.72588651428232
39	24.91469250661356	27.110691674345333	25.22188865440062	22.75272716464049
40	23.759028624082276	27.42829117498439	26.630056772582705	22.182623428350624
41	22.886930416431355	25.39815117557887	25.100318045358616	26.61460036263116
42	21.031715364265967	26.996106173646822	29.641827423238116	22.33035103884909
43	23.147460095710844	24.077519840680083	26.09993163510983	26.675088428499244
44	23.672086318104807	23.49166245578575	28.919537496656066	23.916713729453377
45	21.329994352465594	23.370240466070207	27.517908628838093	27.781856552626106
46	26.31959099961359	26.120143863508012	25.480337663109708	22.07992747376869
47	20.438427013048777	25.713967244300452	32.34610468745356	21.501501055197217
48	22.92661177659543	26.94067116487828	26.613114169366586	23.519602889159707
49	22.261540290699404	24.192551199357965	31.062330945516152	22.483577564426476
50	22.27179502422495	24.393038670748744	28.58677882471837	24.74838748030794
51	22.368992063727966	23.62081265047707	27.19020301399994	26.819992271795023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	640.0
1	956.5
2	1273.0
3	2509.5
4	3746.0
5	2599.0
6	1452.0
7	1466.5
8	1481.0
9	1465.5
10	1450.0
11	1404.5
12	1359.0
13	1371.5
14	1384.0
15	1406.5
16	1429.0
17	1449.0
18	1469.0
19	1556.0
20	1643.0
21	1649.5
22	1656.0
23	2177.5
24	2699.0
25	2905.0
26	3952.0
27	4793.0
28	5987.0
29	7181.0
30	7921.5
31	8662.0
32	9910.0
33	11158.0
34	13596.5
35	16035.0
36	17502.0
37	18969.0
38	21083.5
39	23198.0
40	25051.5
41	26905.0
42	29336.0
43	31767.0
44	35586.5
45	39406.0
46	66148.0
47	92890.0
48	74390.5
49	55891.0
50	55610.0
51	55329.0
52	51074.5
53	46820.0
54	43728.5
55	40637.0
56	39459.5
57	38282.0
58	35609.0
59	32936.0
60	31274.5
61	29613.0
62	25866.0
63	22119.0
64	19660.0
65	17201.0
66	13918.5
67	10636.0
68	8936.0
69	7236.0
70	5830.5
71	4425.0
72	3562.5
73	2700.0
74	2126.0
75	1295.5
76	1039.0
77	674.0
78	309.0
79	234.5
80	160.0
81	146.0
82	132.0
83	96.5
84	61.0
85	36.5
86	12.0
87	8.0
88	4.0
89	4.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672860.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.58346197788706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.19127129039525	19.722608394393255
2	8.11323615715259	4.313558098025687
3	3.031824383227243	2.417891646454569
4	1.682370808511972	1.7889296168314046
5	1.198471032678466	1.5929754564403513
6	0.8966886447176532	1.4302253095712885
7	0.8017487324301589	1.491927986106339
8	0.646737002685338	1.3754006816462656
9	0.6003244569950897	1.4362832139232196
>10	8.56181804324428	50.65256937064171
>50	0.23277737031886722	3.803993287380101
>100	0.033735850770850034	1.6183039419435779
>500	0.002811320897570836	0.44497005958862396
>1k	0.004498113436113338	2.4063060091761024
>5k	0.0	0.0
>10k+	0.0016867925385425015	5.504056927877557
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	12924	1.9207561751330142	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	12535	1.8629432571411586	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	11365	1.68905864518622	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	2635	0.39161192521475496	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	2304	0.3424189281574176	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	2299	0.34167583152513153	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	2264	0.3364741550991291	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1977	0.2938204084059091	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	1744	0.2591921053413786	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	1584	0.2354130131082246	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTT	1292	0.19201616978271854	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	749	0.11131587551645215	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4585797937163747E-4	0.0	0.0	0.0867936866510121	0.0
2	4.4585797937163747E-4	0.0	0.0	0.6753262194215736	0.0
3	4.4585797937163747E-4	0.0	0.0	0.8481704960913117	0.0
4	4.4585797937163747E-4	0.0	0.0	1.3873614124780786	0.0
5	4.4585797937163747E-4	0.0	0.0	3.60312695062866	0.0
6	4.4585797937163747E-4	0.0	0.0	4.2356508040305565	0.0
7	4.4585797937163747E-4	0.0	0.0	4.890764795053949	0.0
8	5.9447730582885E-4	0.0	0.0	5.565496537169693	0.0
9	5.9447730582885E-4	0.0	0.0	5.758850280890527	0.0
10	5.9447730582885E-4	0.0	0.0	8.039265226049995	0.0
11	5.9447730582885E-4	0.0	0.0	8.595250126326427	0.0
12	5.9447730582885E-4	0.0	0.0	11.050590018726036	0.0
13	5.9447730582885E-4	0.0	0.0	11.35689445055435	0.0
14	5.9447730582885E-4	0.0	0.0	11.538210028832149	0.0
15	5.9447730582885E-4	0.0	0.0	11.959248580685433	0.0
16	5.9447730582885E-4	0.0	0.0	12.307909520554054	0.0
17	5.9447730582885E-4	0.0	0.0	12.663109710786792	0.0
18	7.430966322860624E-4	0.0	0.0	13.04803376631097	0.0
19	7.430966322860624E-4	0.0	0.0	13.663466397170287	0.0
20	7.430966322860624E-4	0.0	0.0	13.988794102785127	0.0
21	7.430966322860624E-4	0.0	0.0	14.280979698600007	0.0
22	7.430966322860624E-4	0.0	0.0	14.695924858068544	0.0
23	7.430966322860624E-4	0.0	0.0	14.972951282584788	0.0
24	7.430966322860624E-4	0.0	0.0	15.227387569479536	0.0
25	7.430966322860624E-4	0.0	0.0	15.473055316113307	0.0
26	7.430966322860624E-4	0.0	0.0	15.72035787533811	0.0
27	7.430966322860624E-4	0.0	0.0	16.098445441845257	0.0
28	7.430966322860624E-4	0.0	0.0	16.330588829771422	0.0
29	7.430966322860624E-4	0.0	0.0	16.60583182237018	0.0
30	7.430966322860624E-4	0.0	0.0	16.962220967214577	0.0
31	0.0010403352852004874	0.0	0.0	17.22557441369676	0.0
32	0.0010403352852004874	0.0	0.0	17.487293047587908	0.0
33	0.0010403352852004874	0.0	0.0	17.74737686888803	0.0
34	0.0010403352852004874	0.0	0.0	18.01281098594061	0.0
35	0.0010403352852004874	0.0	0.0	18.295930802841603	0.0
36	0.0010403352852004874	0.0	0.0	18.56136491989418	0.0
37	0.0010403352852004874	0.0	0.0	18.8440388788158	0.0
38	0.0010403352852004874	0.0	0.0	19.155247748417203	0.0
39	0.0010403352852004874	0.0	0.0	19.64063846862646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACGT	20	7.0312E-4	45.0	44
TATAGCG	30	2.1641554E-6	44.999996	1
ACACTCG	105	0.0	42.85714	40
CCGATGA	1355	0.0	41.84502	18
CGATGAA	1405	0.0	41.156586	19
GATGAAT	1490	0.0	41.073826	20
TACGGCT	1385	0.0	40.938625	7
ACGGCTG	1385	0.0	40.938625	8
GCGAACC	55	6.002665E-11	40.909092	33
CCACCGA	1805	0.0	40.761772	15
CGTTTTT	1075	0.0	40.186047	1
AACGGGA	225	0.0	40.0	4
CACCGAT	1555	0.0	39.790997	16
TACGAAA	85	0.0	39.705883	20
ATGAATG	1555	0.0	39.212215	21
CTCGAAT	190	0.0	39.078945	43
TGATACC	1435	0.0	39.04181	4
ACATGCG	35	6.2444287E-6	38.571426	1
AGTTACG	35	6.2444287E-6	38.571426	1
ACCGATG	1480	0.0	38.158783	17
>>END_MODULE