SRR2935627.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935627.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351870
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	8378	2.3809929803620657	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	7761	2.2056441299343508	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	7141	2.0294426919032595	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3539	1.005769176116179	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1758	0.4996163355784807	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1690	0.4802910165686191	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	1479	0.4203256884644897	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	1214	0.3450137834995879	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA	1197	0.3401824537471225	No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1189	0.33790888680478587	Illumina PCR Primer Index 11 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	1188	0.33762469093699377	Illumina PCR Primer Index 11 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	789	0.22423053968795292	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.1412453462926649	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	453	0.12874072810981327	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	444	0.12618296529968456	No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	432	0.12277261488617955	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATG	384	0.1091312132321596	No Hit
AAAGAGAGGGATTGTAACTAGAATTTCTCTCTCTGTGGAGTCATTTTGTAA	356	0.1011737289339813	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.026607E-4	45.0	1
AACGTTC	45	3.8380676E-10	45.0	10
CGGGTTG	20	7.026607E-4	45.0	6
CCGGGTA	20	7.026607E-4	45.0	5
CCGGGCT	40	6.7921064E-9	45.0	5
GATGTAC	20	7.026607E-4	45.0	9
GGTCGGA	20	7.026607E-4	45.0	44
CACGGGT	35	1.2087185E-7	45.0	4
CGTGCGG	35	1.2087185E-7	45.0	2
CGTTGTG	20	7.026607E-4	45.0	20
CGCATGG	20	7.026607E-4	45.0	2
TAGCGAG	20	7.026607E-4	45.0	1
CGCGCGG	20	7.026607E-4	45.0	2
CGTCTGG	20	7.026607E-4	45.0	2
GTTAGCG	20	7.026607E-4	45.0	1
GGTCACG	20	7.026607E-4	45.0	7
CGATTGA	20	7.026607E-4	45.0	10
TACGGGT	20	7.026607E-4	45.0	4
GCGTTGT	20	7.026607E-4	45.0	19
ACGGGCT	45	3.8380676E-10	45.0	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content