##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935627.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351870
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.878435217551935	33.0	31.0	34.0	30.0	34.0
2	31.888237133032085	33.0	31.0	34.0	30.0	34.0
3	31.932634211498566	33.0	31.0	34.0	30.0	34.0
4	35.64695199931793	37.0	35.0	37.0	33.0	37.0
5	35.70110836388439	37.0	35.0	37.0	35.0	37.0
6	35.63026970187853	37.0	35.0	37.0	33.0	37.0
7	35.90819620882712	37.0	35.0	37.0	35.0	37.0
8	35.62692471651462	37.0	35.0	37.0	33.0	37.0
9	37.45209594452497	39.0	37.0	39.0	35.0	39.0
10	37.09607525506579	39.0	37.0	39.0	34.0	39.0
11	37.09851081365277	39.0	37.0	39.0	34.0	39.0
12	37.025907295307924	39.0	37.0	39.0	33.0	39.0
13	36.85675675675676	39.0	35.0	39.0	33.0	39.0
14	37.9071702617444	40.0	37.0	41.0	33.0	41.0
15	37.9279961349362	40.0	37.0	41.0	33.0	41.0
16	37.88621365845341	40.0	36.0	41.0	33.0	41.0
17	37.86175576207122	40.0	36.0	41.0	33.0	41.0
18	37.840716741978575	39.0	36.0	41.0	33.0	41.0
19	37.73904567027596	39.0	36.0	41.0	33.0	41.0
20	37.79456333304914	40.0	36.0	41.0	33.0	41.0
21	37.867124222013814	40.0	36.0	41.0	33.0	41.0
22	37.97752294881632	40.0	36.0	41.0	34.0	41.0
23	37.99790547645437	40.0	36.0	41.0	34.0	41.0
24	37.88781936510643	40.0	36.0	41.0	33.0	41.0
25	37.717151220621254	39.0	36.0	41.0	33.0	41.0
26	37.789660954329726	40.0	36.0	41.0	33.0	41.0
27	37.80273964816551	40.0	36.0	41.0	33.0	41.0
28	37.686492170403845	40.0	36.0	41.0	33.0	41.0
29	37.69056185523063	40.0	36.0	41.0	33.0	41.0
30	37.604692073777244	40.0	36.0	41.0	33.0	41.0
31	37.539662375309064	40.0	36.0	41.0	33.0	41.0
32	37.51280586580271	40.0	36.0	41.0	33.0	41.0
33	37.46666666666667	40.0	36.0	41.0	33.0	41.0
34	37.40873618097593	40.0	36.0	41.0	33.0	41.0
35	37.40123056810754	40.0	36.0	41.0	33.0	41.0
36	37.34319208798704	40.0	36.0	41.0	33.0	41.0
37	37.2925995396027	40.0	36.0	41.0	33.0	41.0
38	37.19415409099952	40.0	35.0	41.0	32.0	41.0
39	37.15651803222781	39.0	35.0	41.0	32.0	41.0
40	37.10608179157075	39.0	35.0	41.0	32.0	41.0
41	36.85401995054992	39.0	35.0	41.0	31.0	41.0
42	37.02487850626652	39.0	35.0	41.0	32.0	41.0
43	37.020197800323984	39.0	35.0	41.0	32.0	41.0
44	36.92643305766334	39.0	35.0	41.0	31.0	41.0
45	36.94237076192912	39.0	35.0	41.0	32.0	41.0
46	36.86012163583141	39.0	35.0	41.0	31.0	41.0
47	36.66531389433597	39.0	35.0	41.0	31.0	41.0
48	36.63508682183761	39.0	35.0	41.0	31.0	41.0
49	36.70443629749624	39.0	35.0	41.0	31.0	41.0
50	36.57032142552647	39.0	35.0	41.0	31.0	41.0
51	35.44878506266519	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	1.0
12	5.0
13	2.0
14	6.0
15	9.0
16	15.0
17	31.0
18	57.0
19	142.0
20	302.0
21	553.0
22	786.0
23	1177.0
24	1498.0
25	1986.0
26	2528.0
27	2776.0
28	3228.0
29	3742.0
30	4879.0
31	6438.0
32	9238.0
33	12898.0
34	22256.0
35	30514.0
36	24457.0
37	36090.0
38	68687.0
39	117494.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.61502827748885	17.761957541137352	22.08258731918038	12.540426862193424
2	27.486571745246824	32.13459516298633	24.006593344132774	16.37223974763407
3	27.469519993179297	31.945604910904596	26.120158012902493	14.464717083013612
4	25.828288856680025	22.81808622502629	33.751101258987696	17.602523659305994
5	29.304288515644984	26.901412453462925	24.104356722653254	19.68994230823884
6	22.322164435729107	37.448205303094895	26.012731974877084	14.216898286298917
7	71.37238184556797	5.858697814533777	17.525790775002132	5.243129564896126
8	69.94657117685509	11.966351209253418	11.263250632335806	6.823826981555688
9	65.68903288146191	6.756188365021172	12.228948191093302	15.325830562423622
10	38.58612555773439	26.098843322818087	21.152698439764688	14.162332679682837
11	29.564327734674738	23.382215022593574	27.72614886179555	19.32730838093614
12	25.18515360786654	19.3216244635803	32.66263108534402	22.83059084320914
13	22.16727768778242	24.972859294625856	35.805553187256656	17.054309830335068
14	18.163526302327565	32.71492312501776	28.20501889902521	20.916531673629464
15	15.0646545599227	25.211583823571203	41.08505982323017	18.638701793275924
16	16.572313638559695	27.03470031545741	31.076249751328618	25.316736294654273
17	16.723505840225084	25.671412737658795	32.279250859692496	25.325830562423622
18	17.716486202290618	25.8615397732117	32.792508596925	23.629465427572683
19	19.03401824537471	26.9562622559468	29.267911444567595	24.74180805411089
20	21.499985790206612	27.067098644385712	33.33958564242476	18.09332992298292
21	20.461818285162135	31.167192429022084	30.31687839258817	18.054110893227612
22	16.4430045187143	25.59183789467701	30.78352800750277	27.18162957910592
23	17.366356893170774	30.05968113223634	30.570096910790916	22.00386506380197
24	21.59433881831358	23.793446443288715	28.777957768494044	25.834256969903656
25	16.474266064171427	32.450620967971126	27.21289112456305	23.8622218432944
26	16.440162560036377	26.574303009634242	30.79574842981783	26.18978600051155
27	20.367749452922954	28.81291386023247	28.342285503168785	22.47705118367579
28	14.667917128484953	27.8392588171768	33.917355841646064	23.57546821269219
29	18.94790689743371	25.15218688720266	28.65802711228579	27.241879103077842
30	17.642026884929095	27.751442294029044	31.20527467530622	23.40125614573564
31	19.15139113877284	31.536078665416206	24.29931508795862	25.013215107852332
32	20.692301133941513	31.681586949725748	24.291357603660444	23.334754312672292
33	17.730411799812433	26.54986216500412	25.12859863017592	30.59112740500753
34	17.979083184130502	29.23153437349021	26.239520277375167	26.54986216500412
35	15.338050984738683	25.513399835166396	27.773893767584617	31.3746554125103
36	17.18702930059397	27.50390769318214	25.70636882939722	29.60269417682667
37	16.11447409554665	29.44866001648336	32.1752351720806	22.261630715889392
38	15.98601756330463	30.16483360331941	24.20212010117373	29.647028732202234
39	19.217040384232813	30.624662517406996	26.389575695569384	23.768721402790803
40	18.111518458521616	24.342512859863017	28.41816579986927	29.1278028817461
41	16.017847500497343	28.56282149657544	25.315315315315317	30.104015687611902
42	17.941285133714153	23.792878051553128	29.927245857845225	28.338590956887487
43	20.670418052121523	24.048654332566006	26.11305311620769	29.167874499104784
44	18.75920084121977	25.604058316992074	29.065563986699633	26.57117685508853
45	17.65794185352545	24.59942592434706	27.069088015460256	30.673544206667238
46	22.970699406030636	27.36720948077415	25.627646574018815	24.0344445391764
47	15.510842072356269	23.535680791201298	35.4198993946628	25.533577741779634
48	18.41816579986927	25.11524142438969	27.589450649387558	28.877142126353483
49	17.56500980475744	21.107511296785745	34.8779378747833	26.44954102367352
50	19.400915110694292	22.144826214226846	30.0	28.454258675078865
51	18.212407991587803	22.444937050615284	26.652741069144852	32.689913888652065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	646.0
1	597.5
2	549.0
3	796.0
4	1043.0
5	783.5
6	524.0
7	554.0
8	584.0
9	584.5
10	585.0
11	612.0
12	639.0
13	741.5
14	844.0
15	778.0
16	712.0
17	662.0
18	612.0
19	974.5
20	1337.0
21	1204.5
22	1072.0
23	1342.5
24	1613.0
25	1745.5
26	2357.5
27	2837.0
28	3397.5
29	3958.0
30	4455.5
31	4953.0
32	6441.5
33	7930.0
34	8110.5
35	8291.0
36	9434.5
37	10578.0
38	11723.5
39	12869.0
40	14819.5
41	16770.0
42	19323.5
43	21877.0
44	24511.5
45	27146.0
46	45313.0
47	63480.0
48	53226.0
49	42972.0
50	41131.5
51	39291.0
52	31237.0
53	23183.0
54	19277.0
55	15371.0
56	13311.0
57	11251.0
58	9999.0
59	8747.0
60	7895.5
61	7044.0
62	6109.0
63	5174.0
64	3948.0
65	2722.0
66	2041.0
67	1360.0
68	1083.0
69	806.0
70	546.0
71	286.0
72	250.0
73	214.0
74	145.0
75	52.0
76	28.0
77	17.5
78	7.0
79	6.5
80	6.0
81	4.5
82	3.0
83	2.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351870.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.224542645300765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.16506544214465	20.213174045789746
2	13.054485368871834	7.630227287500997
3	4.908446644899418	4.303413248881395
4	2.473257160163706	2.891192373999988
5	1.438632918163993	2.1021694533908555
6	0.9474273307689505	1.66128782588284
7	0.7257940614914751	1.4847699651234563
8	0.5401025003669389	1.2627398843845743
9	0.48120036985891373	1.2656574656888695
>10	5.977067650256536	38.961126382062744
>50	0.21963502305530924	3.9790309993718327
>100	0.05690543779160285	3.355830367706029
>500	9.983410138877692E-4	0.23019910973048657
>1k	0.007986728111102154	3.8669949307577554
>5k	0.0029950230416633077	6.792186659728425
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	8378	2.3809929803620657	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	7761	2.2056441299343508	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	7141	2.0294426919032595	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3539	1.005769176116179	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1758	0.4996163355784807	No Hit
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1690	0.4802910165686191	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	1479	0.4203256884644897	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	1214	0.3450137834995879	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA	1197	0.3401824537471225	No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1189	0.33790888680478587	Illumina PCR Primer Index 11 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	1188	0.33762469093699377	Illumina PCR Primer Index 11 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	789	0.22423053968795292	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.1412453462926649	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	453	0.12874072810981327	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	444	0.12618296529968456	No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	432	0.12277261488617955	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATG	384	0.1091312132321596	No Hit
AAAGAGAGGGATTGTAACTAGAATTTCTCTCTCTGTGGAGTCATTTTGTAA	356	0.1011737289339813	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014209793389604116	0.0	0.0	0.3580867934180237	0.0
2	0.0014209793389604116	0.0	0.0	2.2872083439906783	0.0
3	0.0014209793389604116	0.0	0.0	2.913576036604428	0.0
4	0.0014209793389604116	0.0	0.0	3.997214880495638	0.0
5	0.0014209793389604116	0.0	0.0	7.879330434535482	0.0
6	0.0014209793389604116	0.0	0.0	9.090573223065336	0.0
7	0.0014209793389604116	0.0	0.0	10.6496717537727	0.0
8	0.0014209793389604116	0.0	0.0	12.6094864580669	0.0
9	0.0014209793389604116	0.0	0.0	13.190382811833915	0.0
10	0.0014209793389604116	0.0	0.0	16.685423593940943	0.0
11	0.0014209793389604116	0.0	0.0	18.44829056185523	0.0
12	0.0014209793389604116	0.0	0.0	22.38156137209765	0.0
13	0.0014209793389604116	0.0	0.0	23.131838463068746	0.0
14	0.0014209793389604116	0.0	0.0	23.455253360616137	0.0
15	0.0014209793389604116	0.0	0.0	24.2461704606815	0.0
16	0.0014209793389604116	0.0	0.0	25.327251541762582	0.0
17	0.0014209793389604116	0.0	0.0	26.498991104669336	0.0
18	0.0014209793389604116	0.0	0.0	27.845795322136016	0.0
19	0.0014209793389604116	0.0	0.0	29.05817489413704	0.0
20	0.0014209793389604116	0.0	0.0	29.85534430329383	0.0
21	0.0014209793389604116	0.0	0.0	30.641714269474523	0.0
22	0.0014209793389604116	0.0	0.0	31.56023531417853	0.0
23	0.0017051752067524938	0.0	0.0	32.459715235740475	0.0
24	0.0017051752067524938	0.0	0.0	33.12956489612641	0.0
25	0.0017051752067524938	0.0	0.0	33.759911330889246	0.0
26	0.0017051752067524938	0.0	0.0	34.360985591269504	0.0
27	0.0017051752067524938	0.0	0.0	34.98280614999858	0.0
28	0.0017051752067524938	0.0	0.0	35.54153522607781	0.0
29	0.0017051752067524938	0.0	0.0	36.108221786455225	0.0
30	0.0017051752067524938	0.0	0.0	36.73458947906897	0.0
31	0.001989371074544576	0.0	0.0	37.339358285730526	0.0
32	0.001989371074544576	0.0	0.0	37.86199448660017	0.0
33	0.001989371074544576	0.0	0.0	38.418734191604855	0.0
34	0.001989371074544576	0.0	0.0	38.973484525535	0.0
35	0.001989371074544576	0.0	0.0	39.54244465285475	0.0
36	0.001989371074544576	0.0	0.0	40.06422826612101	0.0
37	0.001989371074544576	0.0	0.0	40.574928240543386	0.0
38	0.001989371074544576	0.0	0.0	41.11404780174496	0.0
39	0.001989371074544576	0.0	0.0	41.66652456873277	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.026607E-4	45.0	1
AACGTTC	45	3.8380676E-10	45.0	10
CGGGTTG	20	7.026607E-4	45.0	6
CCGGGTA	20	7.026607E-4	45.0	5
CCGGGCT	40	6.7921064E-9	45.0	5
GATGTAC	20	7.026607E-4	45.0	9
GGTCGGA	20	7.026607E-4	45.0	44
CACGGGT	35	1.2087185E-7	45.0	4
CGTGCGG	35	1.2087185E-7	45.0	2
CGTTGTG	20	7.026607E-4	45.0	20
CGCATGG	20	7.026607E-4	45.0	2
TAGCGAG	20	7.026607E-4	45.0	1
CGCGCGG	20	7.026607E-4	45.0	2
CGTCTGG	20	7.026607E-4	45.0	2
GTTAGCG	20	7.026607E-4	45.0	1
GGTCACG	20	7.026607E-4	45.0	7
CGATTGA	20	7.026607E-4	45.0	10
TACGGGT	20	7.026607E-4	45.0	4
GCGTTGT	20	7.026607E-4	45.0	19
ACGGGCT	45	3.8380676E-10	45.0	5
>>END_MODULE