##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935623.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	705657
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17435949760294	33.0	31.0	34.0	30.0	34.0
2	32.18931010391734	33.0	31.0	34.0	30.0	34.0
3	32.205803952911964	34.0	31.0	34.0	30.0	34.0
4	35.843807969027445	37.0	35.0	37.0	35.0	37.0
5	35.90873753112348	37.0	35.0	37.0	35.0	37.0
6	35.85332250654355	37.0	35.0	37.0	35.0	37.0
7	36.09133899330695	37.0	35.0	37.0	35.0	37.0
8	35.889855836475796	37.0	35.0	37.0	35.0	37.0
9	37.708475930940956	39.0	38.0	39.0	35.0	39.0
10	37.3772385167298	39.0	37.0	39.0	34.0	39.0
11	37.401267187883064	39.0	37.0	39.0	35.0	39.0
12	37.27175950922332	39.0	37.0	39.0	34.0	39.0
13	37.21275208777069	39.0	37.0	39.0	34.0	39.0
14	38.38496323284542	40.0	38.0	41.0	34.0	41.0
15	38.46104552211627	40.0	38.0	41.0	34.0	41.0
16	38.27697025608759	40.0	37.0	41.0	34.0	41.0
17	38.29135401476921	40.0	37.0	41.0	34.0	41.0
18	38.207773748435855	40.0	37.0	41.0	34.0	41.0
19	38.07578186002548	40.0	37.0	41.0	34.0	41.0
20	38.19232856756186	40.0	37.0	41.0	34.0	41.0
21	38.16489739349287	40.0	37.0	41.0	34.0	41.0
22	38.25457410611671	40.0	37.0	41.0	34.0	41.0
23	38.279494145172514	40.0	37.0	41.0	34.0	41.0
24	38.21698785670659	40.0	37.0	41.0	34.0	41.0
25	38.002067576740544	40.0	36.0	41.0	34.0	41.0
26	38.0674973818725	40.0	37.0	41.0	34.0	41.0
27	38.06326303005568	40.0	37.0	41.0	34.0	41.0
28	37.91447686340531	40.0	36.0	41.0	34.0	41.0
29	37.88386000564013	40.0	36.0	41.0	34.0	41.0
30	37.74044613742937	40.0	36.0	41.0	33.0	41.0
31	37.741363013475386	40.0	36.0	41.0	33.0	41.0
32	37.65603969067125	40.0	36.0	41.0	33.0	41.0
33	37.59866620752008	40.0	36.0	41.0	33.0	41.0
34	37.49502945482012	40.0	36.0	41.0	33.0	41.0
35	37.403971051091396	40.0	36.0	41.0	33.0	41.0
36	37.322992615392465	40.0	35.0	41.0	33.0	41.0
37	37.32798087456087	40.0	35.0	41.0	33.0	41.0
38	37.17861652332507	40.0	35.0	41.0	32.0	41.0
39	37.13116145662836	40.0	35.0	41.0	32.0	41.0
40	37.02450057180755	40.0	35.0	41.0	32.0	41.0
41	36.85610431130138	39.0	35.0	41.0	31.0	41.0
42	36.87128165666889	39.0	35.0	41.0	31.0	41.0
43	36.82861361823095	39.0	35.0	41.0	31.0	41.0
44	36.75607837802218	39.0	35.0	41.0	31.0	41.0
45	36.73995156286978	39.0	35.0	41.0	31.0	41.0
46	36.60810847196301	39.0	35.0	41.0	31.0	41.0
47	36.52318194250181	39.0	35.0	41.0	31.0	41.0
48	36.412170502099464	39.0	35.0	41.0	31.0	41.0
49	36.36314810169814	39.0	35.0	41.0	31.0	41.0
50	36.22824686781255	39.0	35.0	41.0	30.0	41.0
51	35.0290070104881	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	13.0
10	18.0
11	17.0
12	11.0
13	20.0
14	12.0
15	38.0
16	44.0
17	72.0
18	141.0
19	326.0
20	667.0
21	1147.0
22	1675.0
23	2247.0
24	3029.0
25	3902.0
26	5082.0
27	5893.0
28	6888.0
29	7863.0
30	9508.0
31	12116.0
32	15781.0
33	21588.0
34	41388.0
35	56694.0
36	46170.0
37	69570.0
38	131258.0
39	262293.0
40	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49739746080603	18.46732902812556	22.76615976317106	14.26911374789735
2	30.407832700589665	27.646434457533903	24.43807685603629	17.507655985840145
3	31.315072336843535	26.31774360631298	25.876311012290675	16.490873044552806
4	28.506484028359385	24.692024595518784	28.22773670494305	18.573754671178776
5	27.138397266660714	29.301912969048704	23.8831330235511	19.676556740739482
6	24.54917899205988	36.42492032247962	24.329100398635596	14.696800286824901
7	77.46695632580702	6.116852805258079	11.535632750755678	4.880558118179229
8	77.90371242685895	8.599503724897508	8.477489771943027	5.019294076300525
9	72.38445873845225	8.03152239685853	10.501986092393329	9.08203277229589
10	40.22846793838933	26.644389554698673	18.15315372766089	14.97398877925111
11	32.5937388844722	25.560860304652262	23.353555622632523	18.491845188243015
12	29.344001405782127	22.016362056920006	27.715023021099487	20.924613516198377
13	26.413115720527113	23.12965080768702	30.30140705753645	20.155826414249418
14	20.98044800802656	28.326084769229244	28.24956033880483	22.443906883939363
15	19.792335369733454	25.186032307480833	34.102120435282295	20.91951188750342
16	23.076508842114514	25.17937184779574	30.192714023952146	21.551405286137598
17	23.112503666795625	25.290048848094752	28.18593169202601	23.41151579308361
18	24.183278845104635	25.80347109147929	27.930425121553387	22.08282494186269
19	25.102847417371326	27.315112016177835	26.06152847629939	21.52051209015145
20	27.28676963453916	25.755997602234515	27.51024931376008	19.44698344946624
21	25.79312612218117	27.868071881948314	26.73891139746364	19.599890598406873
22	22.909147078538155	25.47739199072637	28.29334931843658	23.320111612298895
23	23.1983810831608	27.355499910012938	27.619650906885358	21.826468099940907
24	23.3562481488882	24.39258733350622	28.59505397098023	23.656110546625346
25	22.26846754159599	27.506281380330673	26.91860209705282	23.306648981020526
26	21.32126514723159	26.778165596033197	28.181113487147442	23.719455769587775
27	22.24281768621299	26.73083381869662	27.641474540747137	23.38487395434326
28	20.49621841772986	27.229234599812656	29.671497625616976	22.603049356840504
29	22.389914646917696	25.97437565276048	29.611411776542994	22.024297923778832
30	22.741785314961803	25.919391432381456	28.32636819304563	23.012455059611113
31	25.33454638726747	25.775978981289775	25.93526316609911	22.954211465343644
32	24.908702103146428	27.253325624205527	26.92171975903307	20.91625251361497
33	23.81808725769035	25.66629396434812	26.61037869673227	23.905240081229266
34	23.856916320535333	25.365014447529038	28.99085533056428	21.787213901371345
35	21.468078684119902	26.75520826690588	27.56991002710949	24.20680302186473
36	23.1833596208923	27.801183861281046	27.720833209335417	21.294623308491236
37	23.251381336825112	26.85582372172316	28.04847114107846	21.84432380037327
38	22.568755075057712	27.072076093626222	26.01292129178907	24.346247539526995
39	24.065232825579567	25.06699430459841	28.23269662172982	22.635076248092204
40	23.257191525061042	25.080173512060394	28.55707517958442	23.10555978329415
41	21.310920177933472	26.542923828432226	28.142001000486072	24.00415499314823
42	21.723868678408913	27.122525532943058	28.084749389575958	23.06885639907207
43	22.705223642647915	24.911678053218488	27.956358400752773	24.42673990338082
44	22.60404134019786	24.888295588366585	28.231704638372467	24.27595843306309
45	22.034926316893337	24.7428991705602	27.10863776594011	26.113536746606353
46	22.932246119573673	26.760593319417225	26.904430906233483	23.40272965477562
47	20.813086244450208	25.034825701438518	31.11384142720897	23.038246626902307
48	22.297660194683818	24.99528807905257	28.63714240771366	24.069909318549946
49	21.906677039978344	23.87661427577421	30.22913398435784	23.987574699889606
50	20.98129827947572	24.352482863487502	29.445041996323994	25.221176860712784
51	21.28725428926518	24.176334961603157	27.934251343074614	26.60215940605705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	992.0
2	1353.0
3	2277.5
4	3202.0
5	2298.0
6	1394.0
7	1351.5
8	1309.0
9	1377.0
10	1445.0
11	1410.5
12	1376.0
13	1372.0
14	1368.0
15	1354.0
16	1340.0
17	1410.5
18	1481.0
19	1553.5
20	1626.0
21	1880.5
22	2135.0
23	2482.5
24	2830.0
25	3368.5
26	4862.0
27	5817.0
28	6855.0
29	7893.0
30	9541.0
31	11189.0
32	13196.5
33	15204.0
34	16720.0
35	18236.0
36	19402.5
37	20569.0
38	22425.0
39	24281.0
40	27431.5
41	30582.0
42	33940.0
43	37298.0
44	41352.5
45	45407.0
46	58502.0
47	71597.0
48	66656.5
49	61716.0
50	61969.5
51	62223.0
52	56315.0
53	50407.0
54	46454.5
55	42502.0
56	40404.0
57	38306.0
58	36737.0
59	35168.0
60	33639.5
61	32111.0
62	28267.5
63	24424.0
64	20819.5
65	17215.0
66	13975.5
67	10736.0
68	8576.0
69	6416.0
70	5360.0
71	4304.0
72	3605.0
73	2906.0
74	2333.0
75	1305.0
76	850.0
77	766.5
78	683.0
79	446.0
80	209.0
81	160.0
82	111.0
83	102.5
84	94.0
85	57.0
86	20.0
87	14.5
88	9.0
89	9.5
90	10.0
91	7.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	705657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.737957532552198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03319473218103	20.535296114804044
2	9.118587415346102	5.058619809674702
3	3.1638565999107278	2.6327676002222624
4	1.636591269276499	1.8158279650134894
5	1.0811648108620147	1.4994651804690196
6	0.8060920486059618	1.3415608206936105
7	0.6540097656527252	1.2698626573884793
8	0.5610337353857529	1.2449543941167311
9	0.48831831459117925	1.2190457405247834
>10	8.068635847880389	51.92919661268489
>50	0.33206010940432573	5.649827342392256
>100	0.048756893961948745	2.119877515445735
>500	0.004619074164816197	0.9495388508510545
>1k	0.002052921851029421	1.282233347768433
>5k	0.0010264609255147105	1.4519260479505105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGC	5169	0.7325088534514644	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCG	5030	0.7128108982125877	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTC	4249	0.6021338979135755	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	1867	0.26457613259699825	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1506	0.21341813373919621	No Hit
CCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGC	1385	0.19627099284779997	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCT	900	0.12754071737402164	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTCGATT	890	0.12612359829208808	No Hit
CTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCTGCT	799	0.11322781464649255	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTCTTCT	743	0.10529194778766454	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTCGATTCGTA	735	0.10415825252211768	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	734	0.10401654061392433	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGTCGATTCGTATGCCGTC	706	0.10004860718451034	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.668476327734296E-4	0.0	0.0	0.12031341005616043	0.0
2	5.668476327734296E-4	0.0	0.0	0.7242895627762497	0.0
3	5.668476327734296E-4	0.0	0.0	0.9676089091442442	0.0
4	5.668476327734296E-4	0.0	0.0	1.3332256322831064	0.0
5	5.668476327734296E-4	0.0	0.0	2.6177023681476976	0.0
6	5.668476327734296E-4	0.0	0.0	3.2571064979161264	0.0
7	5.668476327734296E-4	0.0	0.0	3.8538553433183544	0.0
8	5.668476327734296E-4	0.0	0.0	4.648717436374896	0.0
9	5.668476327734296E-4	0.0	0.0	4.958641379593769	0.0
10	5.668476327734296E-4	0.0	0.0	6.329278955639921	0.0
11	5.668476327734296E-4	0.0	0.0	7.170622554583884	0.0
12	5.668476327734296E-4	0.0	0.0	8.61013211801201	0.0
13	5.668476327734296E-4	0.0	0.0	8.972206043446036	0.0
14	5.668476327734296E-4	0.0	0.0	9.14537799525832	0.0
15	5.668476327734296E-4	0.0	0.0	9.48095179386019	0.0
16	5.668476327734296E-4	0.0	0.0	9.981761677415514	0.0
17	5.668476327734296E-4	0.0	0.0	10.551868684077391	0.0
18	5.668476327734296E-4	0.0	0.0	11.234636657752988	0.0
19	5.668476327734296E-4	0.0	0.0	11.734171134134572	0.0
20	5.668476327734296E-4	0.0	0.0	12.13549925813816	0.0
21	5.668476327734296E-4	0.0	0.0	12.578774106966982	0.0
22	5.668476327734296E-4	0.0	0.0	13.08766156928933	0.0
23	5.668476327734296E-4	0.0	0.0	13.535896334904919	0.0
24	5.668476327734296E-4	0.0	0.0	13.91752650366963	0.0
25	5.668476327734296E-4	0.0	0.0	14.262595000120456	0.0
26	5.668476327734296E-4	0.0	0.0	14.593492305751944	0.0
27	5.668476327734296E-4	0.0	0.0	14.9528737049303	0.0
28	5.668476327734296E-4	0.0	0.0	15.305594644423566	0.0
29	5.668476327734296E-4	0.0	0.0	15.664267484060954	0.0
30	5.668476327734296E-4	0.0	0.0	16.096630515958886	0.0
31	7.08559540966787E-4	0.0	0.0	16.479536091897337	0.0
32	7.08559540966787E-4	0.0	0.0	16.848837324649228	0.0
33	7.08559540966787E-4	0.0	0.0	17.2313177648631	0.0
34	7.08559540966787E-4	0.0	0.0	17.621025512394834	0.0
35	7.08559540966787E-4	0.0	0.0	17.998971171546515	0.0
36	8.502714491601444E-4	0.0	0.0	18.357644011183904	0.0
37	8.502714491601444E-4	0.0	0.0	18.745084368184543	0.0
38	8.502714491601444E-4	0.0	0.0	19.151655832791285	0.0
39	8.502714491601444E-4	0.0	0.0	19.553409092519452	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	25	3.8892693E-5	45.0	1
CCAATCG	20	7.031433E-4	45.0	1
ACGATAG	25	3.8892693E-5	45.0	1
ACGATAA	20	7.031433E-4	45.0	40
TCCCGAT	20	7.031433E-4	45.0	12
CGATAGT	20	7.031433E-4	45.0	10
GCGTAAG	45	3.8380676E-10	45.0	1
TGTCGAA	30	2.1643064E-6	44.999996	29
CGAAAGG	60	3.6379788E-12	41.249996	2
TTTACGG	95	0.0	40.263157	2
CCGACGG	45	1.9264917E-8	40.0	2
GGACCGT	45	1.9264917E-8	40.0	8
ATAACGG	85	0.0	39.705883	2
CGCGCAT	40	3.455698E-7	39.375	36
TACGCGC	40	3.455698E-7	39.375	34
ATTGCGG	150	0.0	39.0	2
TCATGCG	35	6.24486E-6	38.57143	1
ATTGCTC	35	6.24486E-6	38.57143	21
CGTATGG	65	9.094947E-12	38.07692	2
TAGGGAT	510	0.0	37.5	5
>>END_MODULE