##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935621.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	310155
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06828843642695	33.0	31.0	34.0	30.0	34.0
2	32.07023907401138	33.0	31.0	34.0	30.0	34.0
3	32.09248279086263	33.0	31.0	34.0	30.0	34.0
4	35.76700037078235	37.0	35.0	37.0	35.0	37.0
5	35.84120842804404	37.0	35.0	37.0	35.0	37.0
6	35.78362109267947	37.0	35.0	37.0	35.0	37.0
7	36.06549305992165	37.0	35.0	37.0	35.0	37.0
8	35.871361093646726	37.0	35.0	37.0	35.0	37.0
9	37.67956021989006	39.0	38.0	39.0	35.0	39.0
10	37.34619464461318	39.0	37.0	39.0	34.0	39.0
11	37.313156325063275	39.0	37.0	39.0	34.0	39.0
12	37.10527316986668	39.0	37.0	39.0	34.0	39.0
13	36.926507713884995	39.0	35.0	39.0	33.0	39.0
14	37.97407102900163	40.0	37.0	41.0	33.0	41.0
15	38.12669471715755	40.0	37.0	41.0	33.0	41.0
16	37.985333139881675	40.0	36.0	41.0	33.0	41.0
17	38.01678193161484	40.0	36.0	41.0	34.0	41.0
18	37.97796907997614	40.0	36.0	41.0	34.0	41.0
19	37.87129660975964	40.0	36.0	41.0	34.0	41.0
20	37.96061001757186	40.0	36.0	41.0	34.0	41.0
21	37.90666924602215	40.0	36.0	41.0	34.0	41.0
22	38.019164611242765	40.0	36.0	41.0	34.0	41.0
23	38.03585948961003	40.0	36.0	41.0	34.0	41.0
24	37.971198271831824	40.0	36.0	41.0	34.0	41.0
25	37.754335735358126	40.0	35.0	41.0	33.0	41.0
26	37.79877158195096	40.0	35.0	41.0	34.0	41.0
27	37.803127468523805	40.0	35.0	41.0	34.0	41.0
28	37.729351453305604	40.0	36.0	41.0	33.0	41.0
29	37.7030710451226	40.0	36.0	41.0	33.0	41.0
30	37.56140316938305	40.0	36.0	41.0	33.0	41.0
31	37.5214263835824	40.0	35.0	41.0	33.0	41.0
32	37.466092760071575	40.0	35.0	41.0	33.0	41.0
33	37.374135512888714	40.0	35.0	41.0	33.0	41.0
34	37.327381470555046	40.0	35.0	41.0	33.0	41.0
35	37.23245151617739	40.0	35.0	41.0	33.0	41.0
36	37.048611178281824	39.0	35.0	41.0	32.0	41.0
37	37.026986506746624	39.0	35.0	41.0	32.0	41.0
38	36.93372346085022	39.0	35.0	41.0	32.0	41.0
39	36.89501700762522	39.0	35.0	41.0	32.0	41.0
40	36.82189227966662	39.0	35.0	41.0	31.0	41.0
41	36.65063919653077	39.0	35.0	41.0	31.0	41.0
42	36.67833825022972	39.0	35.0	41.0	31.0	41.0
43	36.56467250245845	39.0	35.0	41.0	31.0	41.0
44	36.53750544082797	39.0	35.0	41.0	31.0	41.0
45	36.607651013203075	39.0	35.0	41.0	31.0	41.0
46	36.49898599087553	39.0	35.0	41.0	31.0	41.0
47	36.39522174396673	38.0	35.0	40.0	31.0	41.0
48	36.30577291999162	38.0	35.0	40.0	31.0	41.0
49	36.28957134336058	38.0	35.0	40.0	31.0	41.0
50	36.16276377940062	38.0	35.0	40.0	31.0	41.0
51	35.06719543454079	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	6.0
11	8.0
12	4.0
13	6.0
14	7.0
15	17.0
16	12.0
17	38.0
18	64.0
19	133.0
20	254.0
21	496.0
22	721.0
23	931.0
24	1264.0
25	1572.0
26	2082.0
27	2578.0
28	2871.0
29	3441.0
30	4092.0
31	5554.0
32	7373.0
33	10732.0
34	21725.0
35	30824.0
36	21681.0
37	32782.0
38	59324.0
39	99480.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45296706485467	19.301317083393787	23.023327046154343	15.2223888055972
2	27.747739033708953	27.63940610339991	26.972965130338057	17.63988973255308
3	30.743982847286034	26.894939626960713	27.316986667956343	15.044090857796908
4	28.642452967064852	24.487111283068145	28.162370427689382	18.70806532217762
5	26.92363495671519	28.877496735503215	24.795344263352195	19.4035240444294
6	23.305766471602908	36.553336235108254	24.53547419838468	15.605423094904161
7	78.02550337734358	5.889635827247666	11.260176363431187	4.82468443197756
8	78.39177185600748	7.229611000951137	8.798826393254986	5.579790749786397
9	72.41443794231917	7.389531040931148	10.976769679676291	9.2192613370734
10	41.352227112250326	26.791442988183327	17.726942980122843	14.129386919443505
11	33.24112137479647	24.13631893730554	23.978655833373637	18.643903854524353
12	29.937289419806223	21.552772001096226	28.92263545646532	19.587303122632232
13	25.048443520175397	25.81644661540198	30.343215489029678	18.79189437539295
14	18.989215069884413	30.570198771581953	28.762715416485307	21.67787074204833
15	17.581854234173235	24.89626154664603	37.66439360964679	19.857490609533944
16	19.94938014863536	23.61496671019329	33.31076397285228	23.124889168319065
17	19.881349647756767	24.25819348390321	29.32340281472167	26.537054053618352
18	22.095403910947752	24.882397510921958	30.279376440811852	22.742822137318438
19	23.691057696957973	26.71051570988699	27.335686995212072	22.262739597942964
20	25.46887846399381	25.711660298882816	28.970998371781853	19.848462865341524
21	23.578855733423612	28.707259273589013	27.159645983459885	20.554239009527496
22	20.62323676871242	24.544179523141654	28.555722138930534	26.27686156921539
23	20.871499734003965	27.460463316728735	27.41081072367042	24.257226225596877
24	22.005448888458996	24.258515903338655	29.610356112266444	24.125679095935904
25	20.48427399203624	28.064999758185422	27.050023375409072	24.40070287436927
26	18.968902645451468	29.028711450726252	27.844464864342022	24.15792103948026
27	21.59017265560768	28.77657945220938	27.824152439909078	21.809095452273862
28	18.153503893214683	27.27346004417146	32.12651738646806	22.4465186761458
29	21.30644355241734	27.00746400993052	28.989053860166692	22.69703857748545
30	22.865341522786995	28.478663893859522	28.399026293304964	20.256968290048523
31	24.701197788202673	27.462397833341395	25.403427318598766	22.43297705985717
32	27.249278586513192	28.25716174170979	24.199190727217037	20.294368944559977
33	25.107446276861566	26.563814866760165	25.419225870935502	22.909512985442763
34	23.13520659025326	26.916541729135435	27.70260031274685	22.245651367864454
35	24.733117312311588	26.633779884251425	26.5973464880463	22.035756315390692
36	25.362157630861986	27.319243604004452	27.745482097660844	19.573116667472714
37	24.622527445954443	27.69518466573165	28.86685689413358	18.81543099418033
38	24.959133336557528	26.43355741484097	26.59637922973997	22.010930018861536
39	27.47916364398446	23.989295674743275	27.672292885815157	20.85924779545711
40	26.270735599941965	23.50437684383615	28.586351985297675	21.638535570924216
41	22.540987570730763	25.48532185520143	27.508503812609824	24.46518676145798
42	23.647208653737646	24.73150521513437	28.701133304315583	22.9201528268124
43	24.028630845867387	23.80938562976576	29.364350083023005	22.797633441343844
44	21.997388402572906	25.57753381373829	28.684045074237076	23.741032709451726
45	23.06556399219745	25.612032693330754	26.57638922474247	24.74601408972933
46	26.071802808273283	26.126291692863248	26.280085763569826	21.521819735293644
47	22.81472167142235	24.0731247279586	30.957424513549675	22.15472908706937
48	23.136496267995035	24.186293949799296	28.66985861907756	24.007351163128114
49	22.391384952684948	22.750882623204525	31.474907707436607	23.382824716673923
50	22.699940352404443	22.329802840515224	30.454772613693155	24.515484193387177
51	21.554384098273445	23.222582257258466	27.010365784849512	28.212667859618577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	444.0
2	641.0
3	979.5
4	1318.0
5	920.0
6	522.0
7	557.5
8	593.0
9	596.5
10	600.0
11	601.5
12	603.0
13	703.5
14	804.0
15	694.0
16	584.0
17	554.5
18	525.0
19	601.0
20	677.0
21	801.0
22	925.0
23	943.5
24	962.0
25	1255.0
26	2189.5
27	2831.0
28	3335.0
29	3839.0
30	4368.5
31	4898.0
32	5262.5
33	5627.0
34	6562.5
35	7498.0
36	7749.5
37	8001.0
38	9132.5
39	10264.0
40	12463.5
41	14663.0
42	16016.0
43	17369.0
44	20599.5
45	23830.0
46	28968.0
47	34106.0
48	33776.0
49	33446.0
50	31761.5
51	30077.0
52	25871.0
53	21665.0
54	18834.5
55	16004.0
56	14735.0
57	13466.0
58	12660.5
59	11855.0
60	11624.0
61	11393.0
62	10332.5
63	9272.0
64	7713.5
65	6155.0
66	5320.0
67	4485.0
68	3623.0
69	2761.0
70	2495.5
71	2230.0
72	1917.5
73	1605.0
74	1329.0
75	817.0
76	581.0
77	425.5
78	270.0
79	209.5
80	149.0
81	120.5
82	92.0
83	96.5
84	101.0
85	57.0
86	13.0
87	7.0
88	1.0
89	2.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	310155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.290806209798326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41576506955178	22.972384775354257
2	10.787223080886141	6.750818139317438
3	3.9021123132405973	3.663007206074382
4	1.903142709943328	2.3820347890570845
5	1.1509531169500258	1.8007125469523304
6	0.8160741885625966	1.5321371572278377
7	0.6007212776919114	1.3157937160452031
8	0.4801648634724369	1.2019796553336235
9	0.4348274085522926	1.2245490158146732
>10	6.098918083462133	44.21627895729554
>50	0.3554868624420402	6.787896374393449
>100	0.04739824832560536	2.8669536199642116
>500	0.0020607934054611026	0.4562235011526495
>1k	0.005151983513652756	2.829230546017314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC	2278	0.7344714739404491	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG	1986	0.6403249987909272	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	1874	0.6042140220212475	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1421	0.45815801776531095	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	1216	0.39206203349937935	No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	713	0.22988505747126436	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	702	0.2263384436813851	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	465	0.14992503748125938	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	464	0.14960261804581582	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	459	0.14799052086859796	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGGGTT	390	0.12574357982299172	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	355	0.11445889958246684	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	332	0.10704325256726477	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC	311	0.10027244442294982	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTA	311	0.10027244442294982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.44838870887137E-4	0.0	0.0	0.2408473182763457	0.0
2	6.44838870887137E-4	0.0	0.0	1.4102626106301688	0.0
3	6.44838870887137E-4	0.0	0.0	1.863584336863826	0.0
4	6.44838870887137E-4	0.0	0.0	2.493591913720559	0.0
5	6.44838870887137E-4	0.0	0.0	4.50581161032387	0.0
6	6.44838870887137E-4	0.0	0.0	5.477583788750786	0.0
7	6.44838870887137E-4	0.0	0.0	6.569940836033596	0.0
8	6.44838870887137E-4	0.0	0.0	8.127549128661476	0.0
9	6.44838870887137E-4	0.0	0.0	8.75497735003466	0.0
10	6.44838870887137E-4	0.0	0.0	10.626944592220019	0.0
11	6.44838870887137E-4	0.0	0.0	12.074607857361642	0.0
12	6.44838870887137E-4	0.0	0.0	14.136480147023262	0.0
13	6.44838870887137E-4	0.0	0.0	14.708774644935596	0.0
14	6.44838870887137E-4	0.0	0.0	14.941561477325854	0.0
15	6.44838870887137E-4	0.0	0.0	15.401331592268383	0.0
16	6.44838870887137E-4	0.0	0.0	16.138704841127822	0.0
17	6.44838870887137E-4	0.0	0.0	17.053086360045782	0.0
18	6.44838870887137E-4	0.0	0.0	18.153503893214683	0.0
19	6.44838870887137E-4	0.0	0.0	18.852186809820896	0.0
20	9.672583063307056E-4	0.0	0.0	19.45349905692315	0.0
21	9.672583063307056E-4	0.0	0.0	20.119940029985006	0.0
22	9.672583063307056E-4	0.0	0.0	20.854089084490013	0.0
23	9.672583063307056E-4	0.0	0.0	21.537295868194935	0.0
24	9.672583063307056E-4	0.0	0.0	22.09895052473763	0.0
25	9.672583063307056E-4	0.0	0.0	22.590962583224517	0.0
26	9.672583063307056E-4	0.0	0.0	23.027196079379664	0.0
27	9.672583063307056E-4	0.0	0.0	23.484709258274087	0.0
28	9.672583063307056E-4	0.0	0.0	23.94480179265206	0.0
29	9.672583063307056E-4	0.0	0.0	24.41166513517435	0.0
30	9.672583063307056E-4	0.0	0.0	24.96945075849172	0.0
31	0.001289677741774274	0.0	0.0	25.425996679079816	0.0
32	0.001289677741774274	0.0	0.0	25.924134706840128	0.0
33	0.001289677741774274	0.0	0.0	26.399058535248503	0.0
34	0.001289677741774274	0.0	0.0	26.876239299704988	0.0
35	0.001289677741774274	0.0	0.0	27.363092647224775	0.0
36	0.001289677741774274	0.0	0.0	27.834792281278716	0.0
37	0.001289677741774274	0.0	0.0	28.294884815656687	0.0
38	0.001289677741774274	0.0	0.0	28.74885138076123	0.0
39	0.001289677741774274	0.0	0.0	29.240541019812675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	7.025314E-4	45.0	30
TCCGGAA	20	7.025314E-4	45.0	33
CTTCGTT	20	7.025314E-4	45.0	7
CGGAATC	20	7.025314E-4	45.0	20
CGGGTAT	25	3.8841987E-5	45.0	6
TTGGCAA	20	7.025314E-4	45.0	28
ACACGCG	25	3.8841987E-5	45.0	36
CACGGGC	25	3.8841987E-5	45.0	4
GCGCGTT	25	3.8841987E-5	45.0	12
CGGCCTA	25	3.8841987E-5	45.0	31
TCCGAGC	20	7.025314E-4	45.0	13
CGGCCAA	20	7.025314E-4	45.0	31
GACGTAT	20	7.025314E-4	45.0	20
GGTCCGA	20	7.025314E-4	45.0	11
CCGGAAT	20	7.025314E-4	45.0	19
GGATACG	25	3.8841987E-5	45.0	1
TCGGCCT	20	7.025314E-4	45.0	30
ATCCGGA	20	7.025314E-4	45.0	32
CCGAGCG	20	7.025314E-4	45.0	14
AGCGATT	45	3.8198777E-10	45.0	22
>>END_MODULE