##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935620.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	500940
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988940791312334	33.0	31.0	34.0	30.0	34.0
2	31.98147482732463	33.0	31.0	34.0	30.0	34.0
3	31.9798418972332	33.0	31.0	34.0	30.0	34.0
4	35.66750109793588	37.0	35.0	37.0	33.0	37.0
5	35.741174591767475	37.0	35.0	37.0	35.0	37.0
6	35.68397013614405	37.0	35.0	37.0	33.0	37.0
7	35.964211282788355	37.0	35.0	37.0	35.0	37.0
8	35.80540184453228	37.0	35.0	37.0	35.0	37.0
9	37.565103605222184	39.0	37.0	39.0	35.0	39.0
10	37.11389387950653	39.0	37.0	39.0	33.0	39.0
11	37.170842815506845	39.0	37.0	39.0	34.0	39.0
12	37.12848045674133	39.0	37.0	39.0	34.0	39.0
13	37.08865732423045	39.0	37.0	39.0	33.0	39.0
14	38.211628139098494	40.0	37.0	41.0	33.0	41.0
15	38.30831836148042	40.0	37.0	41.0	34.0	41.0
16	38.110296642312456	40.0	37.0	41.0	33.0	41.0
17	38.11140455942827	40.0	37.0	41.0	33.0	41.0
18	38.03364674412105	40.0	37.0	41.0	33.0	41.0
19	37.91842735656965	40.0	37.0	41.0	33.0	41.0
20	38.03483251487204	40.0	37.0	41.0	34.0	41.0
21	37.994170958597834	40.0	36.0	41.0	34.0	41.0
22	38.08018724797381	40.0	37.0	41.0	34.0	41.0
23	38.115075657763406	40.0	37.0	41.0	34.0	41.0
24	38.08269453427556	40.0	37.0	41.0	34.0	41.0
25	37.77914720325788	40.0	36.0	41.0	33.0	41.0
26	37.91670060286661	40.0	36.0	41.0	34.0	41.0
27	37.9143530163293	40.0	36.0	41.0	34.0	41.0
28	37.635670938635364	40.0	36.0	41.0	33.0	41.0
29	37.46201940352138	40.0	36.0	41.0	33.0	41.0
30	37.32812113227133	40.0	36.0	41.0	32.0	41.0
31	37.33705034535074	40.0	36.0	41.0	32.0	41.0
32	37.346155228171035	40.0	36.0	41.0	33.0	41.0
33	37.284339441849326	40.0	36.0	41.0	32.0	41.0
34	37.16205932846249	40.0	36.0	41.0	32.0	41.0
35	37.1105142332415	40.0	35.0	41.0	32.0	41.0
36	37.01614963867928	40.0	35.0	41.0	31.0	41.0
37	37.03001357447998	40.0	35.0	41.0	31.0	41.0
38	36.839807162534434	40.0	35.0	41.0	31.0	41.0
39	36.77063121331896	39.0	35.0	41.0	31.0	41.0
40	36.70212400686709	39.0	35.0	41.0	31.0	41.0
41	36.553110152912524	39.0	35.0	41.0	30.0	41.0
42	36.579470595280874	39.0	35.0	41.0	30.0	41.0
43	36.47284505130355	39.0	35.0	41.0	30.0	41.0
44	36.411153032299275	39.0	35.0	41.0	30.0	41.0
45	36.42870004391744	39.0	35.0	41.0	30.0	41.0
46	36.25577115023756	39.0	35.0	41.0	30.0	41.0
47	36.21954924741486	39.0	35.0	41.0	30.0	41.0
48	36.14851479219068	39.0	35.0	41.0	30.0	41.0
49	36.00505849003873	39.0	35.0	41.0	29.0	41.0
50	35.81424921148241	39.0	35.0	40.0	28.0	41.0
51	34.67496905817064	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	6.0
11	8.0
12	12.0
13	10.0
14	8.0
15	23.0
16	32.0
17	64.0
18	126.0
19	262.0
20	509.0
21	960.0
22	1450.0
23	2053.0
24	2811.0
25	3851.0
26	4683.0
27	5266.0
28	5789.0
29	6442.0
30	7530.0
31	9275.0
32	12271.0
33	16601.0
34	28809.0
35	39885.0
36	34919.0
37	50947.0
38	96069.0
39	170165.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.036491396175194	18.4942308460095	22.564977841657683	15.904299916157624
2	29.51331496786042	26.551083962151157	25.677127001237672	18.25847406875075
3	29.92414261188965	25.68072024593764	27.802730865971974	16.59240627620074
4	27.12779973649539	24.73509801573043	30.120174072743243	18.01692817503094
5	26.814588573481856	29.30071465644588	25.55515630614445	18.329540463927817
6	23.53375653770911	35.26170798898072	25.99892202659001	15.205613446720164
7	78.21834950293449	5.717451191759492	11.445881742324431	4.618317562981595
8	79.54924741486006	6.6544895596278995	8.772108436140057	5.024154589371981
9	73.8132311254841	7.188685271689224	11.023675490078652	7.974408112748034
10	37.10663951770671	29.38096378807841	18.796263025512037	14.716133668702838
11	28.497824090709468	28.118337525452148	25.438775102806723	17.94506328103166
12	26.042839461811795	21.8367469157983	31.61176987263944	20.50864374975047
13	25.228570287858826	25.031341078771906	32.010420409629894	17.72966822373937
14	19.86864694374576	31.064398930011578	28.786880664351024	20.280073461891643
15	18.0484688785084	26.085559148800257	37.06232283307382	18.80364913961752
16	20.79370782928095	27.070108196590407	30.99832315247335	21.137860821655288
17	20.84461212919711	25.708468080009585	28.174232443007146	25.27268734778616
18	24.220265900107798	24.903381642512077	29.590170479498546	21.286181977881583
19	24.288337924701562	27.247574559827527	26.70559348424961	21.758494031221304
20	23.926418333532958	26.66906216313331	27.837465564738288	21.56705393859544
21	23.330937836866692	28.69365592685751	28.69764842096858	19.277757815307222
22	20.603864734299517	26.852716892242583	27.707110632011815	24.83630774144608
23	19.81155427795744	27.654210085040127	28.030303030303028	24.503932606699404
24	21.060206811194952	24.951890445961592	28.88329939713339	25.104603345710064
25	19.48057651614964	28.957160538188205	27.323431947937877	24.23883099772428
26	18.578871721164212	28.28202978400607	28.200982153551323	24.938116341278395
27	21.265421008504013	27.455583503014335	26.952529245019363	24.326466243462292
28	20.386673054657244	27.59013055455743	30.28167045953607	21.74152593124925
29	20.471713179223062	27.655607457979	29.388549526889445	22.484129835908494
30	21.895236954525494	27.513873917035976	27.975206611570247	22.615682516868286
31	20.997923903062244	29.070547370942627	26.077574160578116	23.853954565417016
32	21.74971054417695	31.294965464925937	25.272886972491715	21.6824370184054
33	22.1733141693616	27.26094941509961	25.981754301912403	24.583982113626384
34	21.00491076775662	28.160857587735055	27.449594761847727	23.384636882660597
35	19.227652014213277	29.96865892122809	25.941829360801695	24.861859703756934
36	20.561743921427716	29.21587415658562	27.1986665069669	23.023715415019762
37	19.221663273046673	30.02135984349423	27.350181658482054	23.406795224977042
38	20.38248093584062	28.656326106918996	25.95779933724598	25.003393619994412
39	22.97979797979798	26.452868607018804	25.638599433065835	24.92873398011738
40	23.35629017447199	26.400167684752663	27.103245897712302	23.14029624306304
41	18.611011298758335	27.039565616640715	25.811474428075222	28.53794865652573
42	20.78412584341438	27.762207050744603	27.24837305864974	24.20529404719128
43	20.88294007266339	26.736734938315966	27.191080768155867	25.189244220864776
44	20.698686469437458	25.94662035373498	27.498303190002794	25.85638998682477
45	19.798977921507564	26.260430390865174	26.70259911366631	27.237992573960952
46	22.42384317483132	28.07442009023037	26.011498383039882	23.490238351898434
47	19.276759691779453	26.047031580628417	29.234838503613208	25.44137022397892
48	19.818541142651814	26.27540224378169	27.556393979318884	26.34966263424762
49	21.807202459376374	23.78388629376772	29.498742364355014	24.910168882500898
50	20.013175230566535	24.246017487124206	30.027747834071945	25.713059448237313
51	20.755180261109114	24.61811793827604	26.57483930211203	28.051862498502818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	526.0
2	830.0
3	1949.0
4	3068.0
5	2197.0
6	1326.0
7	1258.5
8	1191.0
9	1206.5
10	1222.0
11	1215.0
12	1208.0
13	1213.5
14	1219.0
15	1176.0
16	1133.0
17	1177.5
18	1222.0
19	1253.5
20	1285.0
21	1449.5
22	1614.0
23	1804.5
24	1995.0
25	2609.5
26	3598.5
27	3973.0
28	4567.5
29	5162.0
30	6285.5
31	7409.0
32	8293.0
33	9177.0
34	10228.0
35	11279.0
36	13081.0
37	14883.0
38	16823.5
39	18764.0
40	20782.5
41	22801.0
42	26802.5
43	30804.0
44	33427.5
45	36051.0
46	47874.5
47	59698.0
48	57346.0
49	54994.0
50	52691.5
51	50389.0
52	43031.5
53	35674.0
54	31450.5
55	27227.0
56	24336.0
57	21445.0
58	20087.0
59	18729.0
60	18277.0
61	17825.0
62	15090.0
63	12355.0
64	9944.0
65	7533.0
66	6216.5
67	4900.0
68	4182.0
69	3464.0
70	2718.5
71	1973.0
72	1771.5
73	1570.0
74	1281.5
75	766.5
76	540.0
77	448.0
78	356.0
79	220.0
80	84.0
81	64.0
82	44.0
83	47.5
84	51.0
85	36.5
86	22.0
87	12.0
88	2.0
89	4.0
90	6.0
91	3.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	500940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.869831201065836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92442226160178	28.75245055085304
2	9.727251990178667	7.367387818365957
3	3.538404091581118	4.01996297008013
4	1.814657366275134	2.748830725944401
5	1.1700333101732623	2.2154481977942875
6	0.8471896926825571	1.9249758394302767
7	0.6719575785010141	1.7812844050477246
8	0.566961507304573	1.7176589263300825
9	0.4626523445932331	1.5768509575070775
>10	5.121943672764665	39.26983845857927
>50	0.11748223604234466	2.853192565894136
>100	0.029635158641310902	1.880818447068592
>500	0.004233594091615843	1.067567380664293
>1k	0.0031751955687118827	2.8237327564407524
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	3175	0.6338084401325508	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	3151	0.6290174471992654	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	3072	0.6132470954605342	No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	1790	0.3573282229408712	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1684	0.3361680041521939	No Hit
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	1218	0.24314289136423525	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	843	0.16828362678165049	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTT	810	0.16169601149838303	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	809	0.1614963867928295	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC	650	0.12975605860981354	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	601	0.11997444803768914	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT	561	0.11198945981554675	No Hit
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	538	0.10739809158781491	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	515	0.10280672336008305	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16828362678165049	0.0
2	0.0	0.0	0.0	0.9837505489679402	0.0
3	0.0	0.0	0.0	1.396973689463808	0.0
4	0.0	0.0	0.0	2.011618157863217	0.0
5	0.0	0.0	0.0	3.7198067632850242	0.0
6	1.9962470555355931E-4	0.0	0.0	4.766638719207889	0.0
7	1.9962470555355931E-4	0.0	0.0	5.672336008304388	0.0
8	1.9962470555355931E-4	0.0	0.0	7.14975845410628	0.0
9	1.9962470555355931E-4	0.0	0.0	7.730865971972691	0.0
10	1.9962470555355931E-4	0.0	0.0	9.484169760849603	0.0
11	1.9962470555355931E-4	0.0	0.0	10.8356290174472	0.0
12	1.9962470555355931E-4	0.0	0.0	12.625663752145966	0.0
13	1.9962470555355931E-4	0.0	0.0	13.27763804048389	0.0
14	1.9962470555355931E-4	0.0	0.0	13.557711502375534	0.0
15	1.9962470555355931E-4	0.0	0.0	13.973929013454706	0.0
16	1.9962470555355931E-4	0.0	0.0	14.690581706391983	0.0
17	1.9962470555355931E-4	0.0	0.0	15.560346548488841	0.0
18	3.9924941110711863E-4	0.0	0.0	16.702599113666306	0.0
19	3.9924941110711863E-4	0.0	0.0	17.362558390226376	0.0
20	3.9924941110711863E-4	0.0	0.0	18.006547690342156	0.0
21	3.9924941110711863E-4	0.0	0.0	18.703637162135188	0.0
22	3.9924941110711863E-4	0.0	0.0	19.418493232722483	0.0
23	3.9924941110711863E-4	0.0	0.0	20.165089631492794	0.0
24	3.9924941110711863E-4	0.0	0.0	20.696091348265263	0.0
25	3.9924941110711863E-4	0.0	0.0	21.21930770152114	0.0
26	3.9924941110711863E-4	0.0	0.0	21.71277997364954	0.0
27	3.9924941110711863E-4	0.0	0.0	22.2545614245219	0.0
28	3.9924941110711863E-4	0.0	0.0	22.75701680840021	0.0
29	3.9924941110711863E-4	0.0	0.0	23.27943466283387	0.0
30	0.001996247055535593	0.0	0.0	23.916037848844173	0.0
31	0.0021958717610891525	0.0	0.0	24.503932606699404	0.0
32	0.0021958717610891525	0.0	0.0	25.084441250449157	0.0
33	0.0021958717610891525	0.0	0.0	25.67692737653212	0.0
34	0.0021958717610891525	0.0	0.0	26.263225136742925	0.0
35	0.0021958717610891525	0.0	0.0	26.81658482053739	0.0
36	0.0021958717610891525	0.0	0.0	27.313650337365754	0.0
37	0.0021958717610891525	0.0	0.0	27.847047550604863	0.0
38	0.002395496466642712	0.0	0.0	28.392621870882742	0.0
39	0.002395496466642712	0.0	0.0	28.989300115782328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	30	2.1630403E-6	45.000004	1
CGTATGG	25	3.887643E-5	45.0	2
CGTATAA	25	3.887643E-5	45.0	33
TTGGACG	35	1.2101009E-7	45.0	1
CGTGCGG	45	3.8380676E-10	45.0	2
GCGAAAG	45	3.8380676E-10	45.0	1
GGCACGC	20	7.029471E-4	45.0	8
CGCATCG	25	3.887643E-5	45.0	21
GCGCCGA	20	7.029471E-4	45.0	22
CACGATG	20	7.029471E-4	45.0	1
TACGCGT	20	7.029471E-4	45.0	29
CGGATAA	25	3.887643E-5	45.0	45
CGTAGCA	25	3.887643E-5	45.0	36
ACGCTTG	20	7.029471E-4	45.0	1
GCGATTA	25	3.887643E-5	45.0	9
ATACGTC	20	7.029471E-4	45.0	40
CGCGTAT	25	3.887643E-5	45.0	31
TTCGCAG	25	3.887643E-5	45.0	1
CCAGTCG	25	3.887643E-5	45.0	28
CCGCATC	25	3.887643E-5	45.0	20
>>END_MODULE