##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935617.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420563
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.987395467504275	33.0	31.0	34.0	30.0	34.0
2	32.01161062670753	33.0	31.0	34.0	30.0	34.0
3	32.043132657889544	33.0	31.0	34.0	30.0	34.0
4	35.72962909243086	37.0	35.0	37.0	35.0	37.0
5	35.74933363134655	37.0	35.0	37.0	35.0	37.0
6	35.67312863946662	37.0	35.0	37.0	35.0	37.0
7	35.895523381752554	37.0	35.0	37.0	35.0	37.0
8	35.61150172506854	37.0	35.0	37.0	33.0	37.0
9	37.36216690483946	39.0	37.0	39.0	34.0	39.0
10	37.12818293573139	39.0	37.0	39.0	34.0	39.0
11	37.138171926679235	39.0	37.0	39.0	34.0	39.0
12	37.02872102396074	39.0	37.0	39.0	33.0	39.0
13	36.86307402220357	39.0	35.0	39.0	33.0	39.0
14	38.00911397341183	40.0	37.0	41.0	33.0	41.0
15	37.99595304389592	40.0	37.0	41.0	33.0	41.0
16	37.96665422302961	40.0	36.0	41.0	33.0	41.0
17	37.824088186549936	40.0	36.0	41.0	33.0	41.0
18	37.810270993882014	40.0	36.0	41.0	33.0	41.0
19	37.66842304244548	40.0	36.0	41.0	33.0	41.0
20	37.52891956734187	40.0	35.0	41.0	32.0	41.0
21	37.709693910305944	40.0	36.0	41.0	33.0	41.0
22	37.73811295810616	40.0	36.0	41.0	33.0	41.0
23	37.68726920818046	40.0	36.0	41.0	33.0	41.0
24	37.64248875911576	40.0	35.0	41.0	33.0	41.0
25	37.54559483359211	39.0	35.0	41.0	33.0	41.0
26	37.43470300525724	40.0	35.0	41.0	33.0	41.0
27	37.37640733968514	40.0	35.0	41.0	33.0	41.0
28	37.25574765255146	40.0	35.0	41.0	32.0	41.0
29	37.17723622857931	40.0	35.0	41.0	32.0	41.0
30	37.10321164724429	39.0	35.0	41.0	31.0	41.0
31	37.156813604620474	39.0	35.0	41.0	32.0	41.0
32	36.92213770588473	39.0	35.0	41.0	31.0	41.0
33	36.751623419083465	39.0	35.0	41.0	31.0	41.0
34	36.546163119437516	39.0	35.0	41.0	30.0	41.0
35	36.49122723587192	39.0	35.0	41.0	30.0	41.0
36	36.41705761086924	39.0	35.0	41.0	30.0	41.0
37	36.31629268385474	39.0	35.0	41.0	30.0	41.0
38	36.25852012659221	39.0	35.0	41.0	30.0	41.0
39	36.10321878053942	39.0	35.0	41.0	30.0	41.0
40	35.95003364537536	39.0	35.0	41.0	29.0	41.0
41	35.895875290979	39.0	35.0	41.0	28.0	41.0
42	35.90810651436289	39.0	35.0	41.0	29.0	41.0
43	35.83093852764033	39.0	35.0	41.0	29.0	41.0
44	35.82418329715168	39.0	35.0	41.0	28.0	41.0
45	35.87497235848137	39.0	35.0	40.0	29.0	41.0
46	35.74826839260705	38.0	35.0	40.0	28.0	41.0
47	35.52373841731203	38.0	35.0	40.0	28.0	41.0
48	35.558035300299835	38.0	35.0	40.0	28.0	41.0
49	35.52561209616633	38.0	35.0	40.0	28.0	41.0
50	35.34933172913452	38.0	35.0	40.0	27.0	41.0
51	34.22068750698468	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	8.0
11	19.0
12	6.0
13	9.0
14	14.0
15	22.0
16	40.0
17	71.0
18	174.0
19	417.0
20	957.0
21	1771.0
22	2611.0
23	3030.0
24	3455.0
25	3712.0
26	4156.0
27	4394.0
28	4791.0
29	5283.0
30	6405.0
31	8404.0
32	11461.0
33	15465.0
34	26684.0
35	36298.0
36	30165.0
37	44020.0
38	76692.0
39	129948.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.06556924884025	16.618437665700505	19.46795129386085	15.848041791598405
2	26.672579375741567	34.44763329156393	23.02770334052211	15.852083992172398
3	28.0554875250557	33.11346932564206	23.66042661860411	15.170616530698137
4	25.11633215475446	22.818460016691912	34.862315515154684	17.202892313398944
5	31.5379622078024	26.319719043282458	23.202706847725548	18.939611901189597
6	22.7033761885853	39.358431435956085	23.76718826905838	14.17100410640023
7	71.26732499054839	5.710202752025261	18.271222147454722	4.751250109971633
8	70.74445445747723	13.851194708046119	10.09171039772876	5.312640436747883
9	65.31221243904005	7.550117342704898	10.853308541169813	16.28436167708524
10	42.33991102403208	23.851598928103517	18.45478560881485	15.35370443904956
11	35.564707309011965	22.639889861923184	23.14207383911566	18.653328989949188
12	27.865741874582405	19.49791113340926	28.0364654047075	24.599881587300832
13	26.724176877186057	22.05448410820733	31.94146893568859	19.27987007891802
14	20.61926512793565	30.291062218977892	26.653557255393366	22.436115397693094
15	18.51446751140733	22.538121518060315	36.998499630257534	21.948911340274822
16	20.255704852780674	23.2835508592054	29.10955076885033	27.351193519163598
17	20.50822350040303	25.58736740987676	30.119387582835387	23.785021506884817
18	21.753459053697068	24.119335272004435	28.485149668420668	25.642056005877834
19	22.989659099825708	26.145428865592073	25.933807776718353	24.931104257863865
20	27.125543616533076	24.37589612020078	29.913235353561774	18.585324909704372
21	23.549860544080197	30.533594253417444	26.01108514063291	19.905460061869444
22	20.883434824271273	23.62048016587289	30.47605233936414	25.020032670491698
23	23.341092773258705	27.78537341611126	27.087023822827973	21.786509987802066
24	23.14207383911566	24.85477799996671	26.585315398644198	25.417832762273427
25	20.25998482985902	32.10981470077016	25.011234939830658	22.618965529540162
26	19.31292101302302	25.84440381108181	29.752498436619483	25.090176739275684
27	24.723525369564133	27.27819613232738	24.952979696264293	23.045298801844194
28	18.697555419758753	28.81304346792276	29.690914321992185	22.7984867903263
29	23.47115652113952	26.17253538708826	27.492670539253332	22.863637552518888
30	20.353193219565203	31.094984580193692	27.991287868880526	20.56053433136058
31	24.260574515589816	24.855015776471063	23.477100933748332	27.407308774190785
32	29.503308660058064	27.61013213240347	23.552476085628076	19.334083121910393
33	24.91755099711577	24.615099283579394	25.515321129057956	24.952028590246883
34	23.592898091368	26.20748853322808	28.35461036753114	21.84500300787278
35	25.289195673418728	26.342545587700293	25.349353129019907	23.01890560986107
36	23.50159191369664	30.296055525569297	23.96121389661002	22.241138664124044
37	24.984603971343176	27.412064304277838	27.722124865953496	19.88120685842549
38	22.975630286068913	24.918026550124477	24.987219512891055	27.11912365091556
39	26.82047636144882	23.918176349322216	27.74138476280605	21.519962526422916
40	24.2306146760414	23.63664896816886	29.69210320451395	22.44063315127579
41	22.286078423446664	25.210016097469346	27.78537341611126	24.718532062972727
42	25.2437803610874	23.801428085685142	28.05192087749041	22.90287067573705
43	26.552740017547904	24.311696464025605	24.365909507017975	24.76965401140852
44	22.6938651284112	24.037540154507173	28.351519272974564	24.917075444107066
45	21.80481877863721	22.68316518571534	27.225409748361123	28.286606287286325
46	27.204009862969404	26.130924498826573	25.037628131813783	21.627437506390244
47	20.146327660778528	24.555892933995622	33.72312828280186	21.574651122423987
48	23.236708887847957	24.661227925423777	26.75413671673447	25.34792646999379
49	22.418282159866653	22.69648066995908	31.595504121855704	23.289733048318563
50	22.838195466553167	22.869581965127697	29.115019628450433	25.1772029398687
51	21.149031179633017	23.183209174368642	26.69302815511588	28.974731490882462
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350.0
1	629.5
2	909.0
3	1663.5
4	2418.0
5	1725.5
6	1033.0
7	1005.5
8	978.0
9	983.5
10	989.0
11	992.0
12	995.0
13	973.5
14	952.0
15	1014.0
16	1076.0
17	1030.5
18	985.0
19	970.5
20	956.0
21	1061.5
22	1167.0
23	1377.0
24	1587.0
25	1824.5
26	2656.5
27	3251.0
28	3993.0
29	4735.0
30	5361.5
31	5988.0
32	6656.0
33	7324.0
34	8272.0
35	9220.0
36	9623.5
37	10027.0
38	11277.0
39	12527.0
40	14281.0
41	16035.0
42	18099.5
43	20164.0
44	22290.5
45	24417.0
46	43376.5
47	62336.0
48	48821.0
49	35306.0
50	34944.0
51	34582.0
52	30304.0
53	26026.0
54	24377.5
55	22729.0
56	22809.5
57	22890.0
58	21421.0
59	19952.0
60	19444.5
61	18937.0
62	17022.5
63	15108.0
64	12860.0
65	10612.0
66	8719.0
67	6826.0
68	5993.5
69	5161.0
70	4312.0
71	3463.0
72	3142.0
73	2821.0
74	2148.5
75	1260.5
76	1045.0
77	813.5
78	582.0
79	425.0
80	268.0
81	220.5
82	173.0
83	132.5
84	92.0
85	55.0
86	18.0
87	12.5
88	7.0
89	4.5
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420563.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.5792075219455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32391474444205	21.984424980692367
2	8.324417472570879	4.924593438409662
3	3.0644581110482263	2.719327272270138
4	1.6706635103985215	1.9766761069367926
5	1.205588101115122	1.7830170314436202
6	1.0643545601877167	1.8889658647632903
7	0.9150047098668466	1.8945579937696289
8	0.7303553082110767	1.728266498106392
9	0.670693175931803	1.785471537099555
>10	7.8781939677164186	46.10803616323357
>50	0.10963207427269611	2.0603891881092884
>100	0.03224472772726356	1.7260915788361464
>500	0.002418354579544767	0.5364975897750144
>1k	0.005642827352271123	2.650298093488571
>5k	0.002418354579544767	6.233386663065967
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGC	9326	2.2175036795914047	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	8622	2.0501090205272456	No Hit
GAATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTC	8194	1.948340676664376	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	2476	0.5887346247767873	No Hit
GAACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCT	1664	0.3956601032425582	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCT	1630	0.3875757020945732	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAACGTTGGT	1605	0.38163128948576075	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAACGTTGGTCGTA	1369	0.325516034458571	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTC	1246	0.29626952442321364	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1125	0.2674985673965613	No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	977	0.23230764475239146	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTT	696	0.16549244702933924	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	577	0.13719704301139188	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATG	443	0.10533499142815703	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.377765043524989E-4	0.0	0.0	0.14337923212455683	0.0
2	2.377765043524989E-4	0.0	0.0	1.10661185125653	0.0
3	2.377765043524989E-4	0.0	0.0	1.3610327109137037	0.0
4	2.377765043524989E-4	0.0	0.0	2.038457971813973	0.0
5	2.377765043524989E-4	0.0	0.0	4.697512619987968	0.0
6	2.377765043524989E-4	0.0	0.0	5.384924494071043	0.0
7	2.377765043524989E-4	0.0	0.0	6.262557571636116	0.0
8	2.377765043524989E-4	0.0	0.0	7.270016620577654	0.0
9	2.377765043524989E-4	0.0	0.0	7.603141503175506	0.0
10	2.377765043524989E-4	0.0	0.0	10.295484861958851	0.0
11	2.377765043524989E-4	0.0	0.0	11.087756174461378	0.0
12	2.377765043524989E-4	0.0	0.0	14.067095773998188	0.0
13	2.377765043524989E-4	0.0	0.0	14.506506754041606	0.0
14	2.377765043524989E-4	0.0	0.0	14.731205550654717	0.0
15	2.377765043524989E-4	0.0	0.0	15.290218112387443	0.0
16	2.377765043524989E-4	0.0	0.0	15.786219900466754	0.0
17	2.377765043524989E-4	0.0	0.0	16.27485061691114	0.0
18	2.377765043524989E-4	0.0	0.0	16.872620748853322	0.0
19	2.377765043524989E-4	0.0	0.0	17.62684782065945	0.0
20	2.377765043524989E-4	0.0	0.0	18.032018984076107	0.0
21	2.377765043524989E-4	0.0	0.0	18.43195906439701	0.0
22	2.377765043524989E-4	0.0	0.0	18.90085433098014	0.0
23	2.377765043524989E-4	0.0	0.0	19.28082118493543	0.0
24	2.377765043524989E-4	0.0	0.0	19.605861666385298	0.0
25	4.755530087049978E-4	0.0	0.0	19.897375660721462	0.0
26	4.755530087049978E-4	0.0	0.0	20.178903041874822	0.0
27	4.755530087049978E-4	0.0	0.0	20.540798881499324	0.0
28	4.755530087049978E-4	0.0	0.0	20.81162631995682	0.0
29	4.755530087049978E-4	0.0	0.0	21.121211328623772	0.0
30	4.755530087049978E-4	0.0	0.0	21.527809151066545	0.0
31	4.755530087049978E-4	0.0	0.0	21.83763193623785	0.0
32	4.755530087049978E-4	0.0	0.0	22.14959470994833	0.0
33	4.755530087049978E-4	0.0	0.0	22.45038198795424	0.0
34	4.755530087049978E-4	0.0	0.0	22.771618045334467	0.0
35	4.755530087049978E-4	0.0	0.0	23.080251947984014	0.0
36	4.755530087049978E-4	0.0	0.0	23.372003718824526	0.0
37	4.755530087049978E-4	0.0	0.0	23.668511019752096	0.0
38	4.755530087049978E-4	0.0	0.0	23.992124842175844	0.0
39	4.755530087049978E-4	0.0	0.0	24.317640876634417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTC	20	7.0281787E-4	45.000004	29
AAGACGG	40	6.7975634E-9	45.000004	2
TGTAGCG	20	7.0281787E-4	45.000004	1
TAGGCGA	20	7.0281787E-4	45.000004	14
CAGTAAG	20	7.0281787E-4	45.000004	1
GTATGCG	25	3.8865728E-5	45.0	1
GGTCGAA	135	0.0	43.333336	42
CGATGAA	845	0.0	42.60355	19
ATGAATG	875	0.0	42.171425	21
CGGTCGA	140	0.0	41.785713	41
CCGATGA	835	0.0	41.766468	18
GATGAAT	880	0.0	41.676136	20
GTCGAAT	135	0.0	41.666668	43
CGGCGAA	245	0.0	41.32653	31
TAAGGGA	230	0.0	41.086956	4
TATGGGT	55	6.002665E-11	40.909092	4
TACGGCT	985	0.0	40.431473	7
ACGGCTG	985	0.0	40.431473	8
GCCCGCT	45	1.9232175E-8	40.0	23
CCCGCTA	45	1.9232175E-8	40.0	24
>>END_MODULE