SRR2935616.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935616.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131979
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	27061	2.390592051619332	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	17216	1.5208762706728658	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	16654	1.4712287065396088	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	7579	0.6695353889073914	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	5678	0.5015994112964993	TruSeq Adapter, Index 19 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	5015	0.44302942015708774	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4556	0.4024809647528797	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	3706	0.32739123252286484	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	3330	0.29417506861876414	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	2899	0.25610015733507424	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	2502	0.22102883534058493	No Hit
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1517	0.1340130868152148	TruSeq Adapter, Index 19 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATC	20	7.03324E-4	45.000004	13
ATACCGG	40	6.8157533E-9	45.000004	2
AGTCGTA	20	7.03324E-4	45.000004	14
TACGACT	25	3.8907678E-5	45.0	30
ACGTAAG	30	2.1654723E-6	44.999996	1
GATGAAT	1565	0.0	40.97444	20
GCGCATC	55	6.184564E-11	40.909092	25
GTTTACG	100	0.0	40.5	1
CGCATCG	50	1.0822987E-9	40.5	21
CGATGAA	1505	0.0	40.066444	19
TCGATAG	45	1.9283107E-8	40.000004	1
TAGCCGT	85	0.0	39.705883	44
TACGGCT	1950	0.0	39.692307	7
CGTTGAT	40	3.4581535E-7	39.375004	25
CCGATGA	1515	0.0	39.356438	18
CGTTTTT	2265	0.0	39.039734	1
GCGAACC	70	0.0	38.571426	33
CACCGAT	1640	0.0	38.55183	16
GTTCGAC	30	1.139911E-4	37.499996	32
ACCCGTA	30	1.139911E-4	37.499996	40