##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935616.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131979
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00555487336779	33.0	31.0	34.0	30.0	34.0
2	32.026738128534184	33.0	31.0	34.0	30.0	34.0
3	32.0546193878155	33.0	31.0	34.0	30.0	34.0
4	35.713492034746224	37.0	35.0	37.0	35.0	37.0
5	35.73503130358425	37.0	35.0	37.0	35.0	37.0
6	35.644267252307685	37.0	35.0	37.0	33.0	37.0
7	35.89503162161135	37.0	35.0	37.0	35.0	37.0
8	35.62929082606656	37.0	35.0	37.0	33.0	37.0
9	37.45259850226903	39.0	37.0	39.0	35.0	39.0
10	37.14564669485918	39.0	37.0	39.0	34.0	39.0
11	37.14648063259124	39.0	37.0	39.0	34.0	39.0
12	37.051539825385454	39.0	37.0	39.0	33.0	39.0
13	36.94364471425707	39.0	37.0	39.0	33.0	39.0
14	38.12301199933921	40.0	37.0	41.0	33.0	41.0
15	38.11360546441232	40.0	37.0	41.0	33.0	41.0
16	38.02441211365228	40.0	37.0	41.0	33.0	41.0
17	37.93359064081577	40.0	36.0	41.0	33.0	41.0
18	37.85286564503405	40.0	36.0	41.0	33.0	41.0
19	37.72661771994004	40.0	36.0	41.0	33.0	41.0
20	37.71351942041328	40.0	36.0	41.0	33.0	41.0
21	37.8208305984475	40.0	36.0	41.0	33.0	41.0
22	37.90988525405507	40.0	36.0	41.0	33.0	41.0
23	37.92280068799863	40.0	36.0	41.0	34.0	41.0
24	37.836151554048264	40.0	36.0	41.0	33.0	41.0
25	37.66183559942366	40.0	36.0	41.0	33.0	41.0
26	37.63566285240274	40.0	36.0	41.0	33.0	41.0
27	37.62651868983435	40.0	36.0	41.0	33.0	41.0
28	37.4291113174361	40.0	35.0	41.0	33.0	41.0
29	37.41516671245668	40.0	35.0	41.0	33.0	41.0
30	37.25841380449637	40.0	35.0	41.0	32.0	41.0
31	37.237916074414805	40.0	35.0	41.0	32.0	41.0
32	37.115442954330426	40.0	35.0	41.0	32.0	41.0
33	37.00107864191827	40.0	35.0	41.0	31.0	41.0
34	36.92776544441195	40.0	35.0	41.0	31.0	41.0
35	36.78874166393546	40.0	35.0	41.0	31.0	41.0
36	36.72591541009153	39.0	35.0	41.0	31.0	41.0
37	36.69021510116354	39.0	35.0	41.0	31.0	41.0
38	36.53556382229706	39.0	35.0	41.0	30.0	41.0
39	36.46342732506522	39.0	35.0	41.0	30.0	41.0
40	36.28123489923399	39.0	35.0	41.0	30.0	41.0
41	36.191922288311005	39.0	35.0	41.0	30.0	41.0
42	36.205983503227536	39.0	35.0	41.0	30.0	41.0
43	36.217456330903666	39.0	35.0	41.0	30.0	41.0
44	36.168594117028675	39.0	35.0	41.0	30.0	41.0
45	36.202567362115374	39.0	35.0	41.0	30.0	41.0
46	36.055736899712805	39.0	35.0	41.0	30.0	41.0
47	35.908664383349866	38.0	35.0	40.0	30.0	41.0
48	35.85051577811956	38.0	35.0	40.0	29.0	41.0
49	35.82451087873538	38.0	35.0	40.0	29.0	41.0
50	35.70002358701001	38.0	35.0	40.0	29.0	41.0
51	34.58804889489999	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	22.0
10	30.0
11	27.0
12	32.0
13	26.0
14	34.0
15	62.0
16	110.0
17	158.0
18	405.0
19	808.0
20	1719.0
21	3120.0
22	4849.0
23	6370.0
24	7564.0
25	8743.0
26	10642.0
27	11625.0
28	12583.0
29	14210.0
30	17012.0
31	22500.0
32	29779.0
33	40582.0
34	71693.0
35	93498.0
36	83319.0
37	119184.0
38	204405.0
39	366626.0
40	228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.12103404745141	16.39862576955933	19.531722761641337	15.948617421347922
2	28.143896662393914	31.900326772846494	22.6692367967957	17.286539767963895
3	28.76237103338489	31.334150191832183	24.239760631601822	15.663718143181102
4	25.873448182342607	22.33628009000167	33.90301410185172	17.88725762580401
5	29.85938785083469	26.397839535892448	23.922793620729713	19.819978992543145
6	23.35184663319726	37.07833802570543	25.121049065397855	14.448766275699459
7	73.71947712810926	5.529696222279742	16.13934534121216	4.61148130839883
8	73.17865437433026	10.587740585293544	10.634296219276154	5.599308821100038
9	67.81194704142038	6.897566120926271	11.58042684537434	13.710059992279009
10	40.90658925651447	23.131789547332595	19.669976209806013	16.29164498634692
11	32.732055983370714	24.56591509206443	23.49946421267532	19.20256471188953
12	27.519061749378743	19.71052466521022	29.175187878927083	23.595225706483955
13	25.821503755811726	21.227778960563757	32.94628257237988	20.00443471124464
14	20.845263030497915	28.44867263438633	28.584187515846143	22.121876819269616
15	19.01219015547108	23.466689753078455	35.89828079849538	21.62283929295508
16	21.89245560209156	24.53314063246756	28.536218428080378	25.038185337360495
17	21.206047108647773	25.785549025202766	29.075981091522017	23.932422774627444
18	22.191489418089912	24.935003211190313	27.686556022682403	25.186951348037372
19	23.489481695331804	27.041049348088613	24.948254340407374	24.52121461617221
20	26.457204594784884	25.678744923713246	28.411216109132763	19.4528343723691
21	24.352660252531187	28.63136153585888	27.318616334755326	19.697361876854604
22	21.665684610756912	23.47658392956053	29.292681224651695	25.56505023503086
23	23.785158558595167	26.428758837398924	28.056615891284203	21.729466712721702
24	24.678991394716686	24.444711430159042	26.62363877775118	24.252658397373096
25	21.69209852832959	29.968842178167616	24.958148516889448	23.380910776613348
26	21.143413437881797	24.357342318187882	28.370137608559876	26.12910663537044
27	24.790124198417107	25.401619641353772	25.66602383966487	24.14223232056425
28	20.943144704981275	26.64316210813098	28.21350926121421	24.20018392567353
29	22.766765107833272	24.494447335153744	27.075325602330082	25.663461954682905
30	22.259158517958372	26.541923480912633	29.897197739534036	21.30172026159496
31	25.93413835415675	24.906469112942908	24.42209616962859	24.737296363271756
32	26.12097927611731	29.719279244579628	23.716252686666447	20.443488792636614
33	23.4262296385357	27.558550114445584	23.94911919744094	25.066101049577778
34	22.64838835349419	26.16002593687692	24.946134159732644	26.24545154989624
35	21.10313000506193	27.642562273681754	25.99226664098892	25.262041080267387
36	21.740244297818244	28.17817291663538	24.1271260332568	25.954456752289573
37	21.155781158484388	27.7092596240743	28.43197621157283	22.702983005868482
38	23.335591914690998	28.568639524231454	23.290979779660223	24.804788781417322
39	23.92685730035628	26.404288418778087	24.059191910804	25.609662370061635
40	21.945636800682696	27.34246836734604	28.07534415391098	22.63655067806028
41	19.636141659871782	26.595634724672458	25.915763454975753	27.852460160480007
42	21.523809187272906	26.76772272277136	27.997692536699002	23.71077555325673
43	23.39831392631842	24.075269947587365	25.25356035756847	27.272855768525737
44	22.51384522151029	24.01113448217679	27.20032792127769	26.274692375035226
45	21.868515228639403	23.469781683229105	26.29518745489095	28.366515633240546
46	24.802491919019698	26.55685308649719	24.975463325733074	23.665191668750037
47	19.79824714062717	25.104087620000016	31.498994239292426	23.59867100008039
48	22.04802385909986	24.621569834776086	26.881152388869406	26.44925391725465
49	20.936165776926956	23.296280231347048	31.252081531547848	24.51547246017815
50	20.95931108262609	23.56881178891128	29.501165657666796	25.970711470795838
51	20.242513332844513	23.084615527319855	27.015165475684622	29.657705664151013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	531.0
1	1433.0
2	2335.0
3	5309.0
4	8283.0
5	5542.0
6	2801.0
7	2752.0
8	2703.0
9	2801.5
10	2900.0
11	2790.5
12	2681.0
13	2673.5
14	2666.0
15	2672.5
16	2679.0
17	2800.5
18	2922.0
19	2938.5
20	2955.0
21	2962.0
22	2969.0
23	3290.5
24	3612.0
25	4574.0
26	6511.5
27	7487.0
28	8934.0
29	10381.0
30	12112.5
31	13844.0
32	16584.5
33	19325.0
34	21413.5
35	23502.0
36	25290.0
37	27078.0
38	30550.0
39	34022.0
40	36776.0
41	39530.0
42	45766.0
43	52002.0
44	56680.5
45	61359.0
46	111081.5
47	160804.0
48	128924.0
49	97044.0
50	96182.5
51	95321.0
52	84587.5
53	73854.0
54	68499.5
55	63145.0
56	61565.5
57	59986.0
58	58192.0
59	56398.0
60	54593.0
61	52788.0
62	49116.5
63	45445.0
64	37704.0
65	29963.0
66	25003.0
67	20043.0
68	17574.0
69	15105.0
70	12488.0
71	9871.0
72	8499.5
73	7128.0
74	6013.5
75	3874.5
76	2850.0
77	2217.0
78	1584.0
79	1217.5
80	851.0
81	675.5
82	500.0
83	337.5
84	175.0
85	119.0
86	63.0
87	43.5
88	24.0
89	24.5
90	25.0
91	14.0
92	3.0
93	3.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1131979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.076332141903592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86518947169598	19.261332143885845
2	8.985710059359283	4.686287200773938
3	3.387810485495412	2.650250143608061
4	1.7963915417588805	1.8737320999924336
5	1.1958128792408287	1.559120690932495
6	0.8439509637852805	1.3204287385886775
7	0.6800115099319506	1.241254419531204
8	0.5999904388737276	1.251643997283026
9	0.5362200510512448	1.2584386937124685
>10	7.473201438230938	43.98601051548591
>50	0.5794082752085217	9.249582836710474
>100	0.048454604377325475	2.2964001247593746
>500	0.0034122960829084605	0.5750788130159403
>1k	0.0023886072580359225	1.7429434920866842
>5k	0.0010236888248725384	1.6258455935985243
>10k+	0.0010236888248725384	5.421650496035009
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	27061	2.390592051619332	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	17216	1.5208762706728658	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	16654	1.4712287065396088	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	7579	0.6695353889073914	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	5678	0.5015994112964993	TruSeq Adapter, Index 19 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	5015	0.44302942015708774	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4556	0.4024809647528797	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	3706	0.32739123252286484	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	3330	0.29417506861876414	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	2899	0.25610015733507424	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	2502	0.22102883534058493	No Hit
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1517	0.1340130868152148	TruSeq Adapter, Index 19 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4170430723538157E-4	0.0	0.0	0.10132696807979653	0.0
2	4.4170430723538157E-4	0.0	0.0	0.8455103849099674	0.0
3	4.4170430723538157E-4	0.0	0.0	1.111504718727114	0.0
4	4.4170430723538157E-4	0.0	0.0	2.0453559650841577	0.0
5	5.300451686824579E-4	0.0	0.0	5.094882502237232	0.0
6	5.300451686824579E-4	0.0	0.0	6.135714531806685	0.0
7	5.300451686824579E-4	0.0	0.0	6.937584531161797	0.0
8	5.300451686824579E-4	0.0	0.0	7.942461830122291	0.0
9	5.300451686824579E-4	0.0	0.0	8.281425715494722	0.0
10	5.300451686824579E-4	0.0	0.0	10.37846108452542	0.0
11	6.183860301295342E-4	0.0	0.0	11.122909523939931	0.0
12	6.183860301295342E-4	0.0	0.0	13.365177269189623	0.0
13	6.183860301295342E-4	0.0	0.0	13.8160690260155	0.0
14	6.183860301295342E-4	0.0	0.0	14.044253471133299	0.0
15	6.183860301295342E-4	0.0	0.0	14.467141174880453	0.0
16	6.183860301295342E-4	0.0	0.0	14.8811947924829	8.834086144707631E-5
17	7.067268915766105E-4	0.0	0.0	15.330319732079836	8.834086144707631E-5
18	7.950677530236868E-4	0.0	0.0	15.854622744768234	8.834086144707631E-5
19	7.950677530236868E-4	0.0	0.0	16.450393514367317	8.834086144707631E-5
20	8.834086144707631E-4	0.0	0.0	16.83723814664406	8.834086144707631E-5
21	8.834086144707631E-4	0.0	0.0	17.20597290232416	8.834086144707631E-5
22	8.834086144707631E-4	0.0	0.0	17.6973247736928	8.834086144707631E-5
23	8.834086144707631E-4	0.0	0.0	18.071359981059718	8.834086144707631E-5
24	8.834086144707631E-4	0.0	0.0	18.392390671558395	8.834086144707631E-5
25	8.834086144707631E-4	0.0	0.0	18.659886800020143	8.834086144707631E-5
26	8.834086144707631E-4	0.0	0.0	18.95609370845219	8.834086144707631E-5
27	8.834086144707631E-4	0.0	0.0	19.28905041524622	8.834086144707631E-5
28	9.717494759178395E-4	0.0	0.0	19.578720099931182	8.834086144707631E-5
29	9.717494759178395E-4	0.0	0.0	19.88641132035135	8.834086144707631E-5
30	9.717494759178395E-4	0.0	0.0	20.275994519332954	8.834086144707631E-5
31	9.717494759178395E-4	0.0	0.0	20.602944047548586	8.834086144707631E-5
32	0.0010600903373649158	0.0	0.0	20.914433925010975	8.834086144707631E-5
33	0.0010600903373649158	0.0	0.0	21.240941748919372	8.834086144707631E-5
34	0.0010600903373649158	0.0	0.0	21.571601593315776	8.834086144707631E-5
35	0.0010600903373649158	0.0	0.0	21.89033542141683	8.834086144707631E-5
36	0.0010600903373649158	0.0	0.0	22.202178662325007	8.834086144707631E-5
37	0.0010600903373649158	0.0	0.0	22.527626395896036	8.834086144707631E-5
38	0.0011484311988119921	0.0	0.0	22.85934633062981	8.834086144707631E-5
39	0.0011484311988119921	0.0	0.0	23.196278376188957	8.834086144707631E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATC	20	7.03324E-4	45.000004	13
ATACCGG	40	6.8157533E-9	45.000004	2
AGTCGTA	20	7.03324E-4	45.000004	14
TACGACT	25	3.8907678E-5	45.0	30
ACGTAAG	30	2.1654723E-6	44.999996	1
GATGAAT	1565	0.0	40.97444	20
GCGCATC	55	6.184564E-11	40.909092	25
GTTTACG	100	0.0	40.5	1
CGCATCG	50	1.0822987E-9	40.5	21
CGATGAA	1505	0.0	40.066444	19
TCGATAG	45	1.9283107E-8	40.000004	1
TAGCCGT	85	0.0	39.705883	44
TACGGCT	1950	0.0	39.692307	7
CGTTGAT	40	3.4581535E-7	39.375004	25
CCGATGA	1515	0.0	39.356438	18
CGTTTTT	2265	0.0	39.039734	1
GCGAACC	70	0.0	38.571426	33
CACCGAT	1640	0.0	38.55183	16
GTTCGAC	30	1.139911E-4	37.499996	32
ACCCGTA	30	1.139911E-4	37.499996	40
>>END_MODULE