SRR2935615.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935615.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231553
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	2270	0.9803371150449358	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	2106	0.9095109974822179	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1496	0.6460723894745479	RNA PCR Primer, Index 30 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1101	0.47548509412531903	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	979	0.422797372523785	RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	712	0.3074889981991164	RNA PCR Primer, Index 30 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.2586880757321218	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	493	0.21291021925865786	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	414	0.17879276018881207	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	368	0.1589268979456107	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	359	0.1550400988110713	No Hit
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	309	0.13344677028585247	Illumina Single End Adapter 2 (95% over 22bp)
AGTGGGGGTACTTGATGTAAGTGAAGAGCAGACACTTCCCAAATGGTTTCT	271	0.11703584060668616	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	266	0.11487650775416428	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	266	0.11487650775416428	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAA	60	0.0	45.000004	5
GATTGCG	30	2.1579708E-6	45.000004	1
GTTGACG	25	3.8811286E-5	45.0	1
CGGGTTC	20	7.021608E-4	45.0	6
AGGTATA	20	7.021608E-4	45.0	6
AGACTGG	20	7.021608E-4	45.0	2
GTCGATG	25	3.8811286E-5	45.0	1
ATTAGCG	25	3.8811286E-5	45.0	1
TTGTGCG	20	7.021608E-4	45.0	1
GCGCGAC	20	7.021608E-4	45.0	9
GGCACCG	25	3.8811286E-5	45.0	8
GGCGTAG	20	7.021608E-4	45.0	1
TAGAACG	20	7.021608E-4	45.0	1
CCGTGGC	20	7.021608E-4	45.0	27
CGTCTAC	20	7.021608E-4	45.0	35
CGATTGA	25	3.8811286E-5	45.0	10
AAGCGGC	20	7.021608E-4	45.0	22
TACGGGT	20	7.021608E-4	45.0	4
GGATAGT	20	7.021608E-4	45.0	8
CGACAGG	35	1.2063174E-7	45.0	2