##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935615.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231553
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06530686279167	33.0	31.0	34.0	30.0	34.0
2	32.07720478680907	33.0	31.0	34.0	30.0	34.0
3	32.085449119639996	33.0	31.0	34.0	30.0	34.0
4	35.74229226138292	37.0	35.0	37.0	33.0	37.0
5	35.8273829317694	37.0	35.0	37.0	35.0	37.0
6	35.761635565075814	37.0	35.0	37.0	35.0	37.0
7	36.042137221284115	37.0	35.0	37.0	35.0	37.0
8	35.82872171813796	37.0	35.0	37.0	35.0	37.0
9	37.64227628231981	39.0	37.0	39.0	35.0	39.0
10	37.28078236947913	39.0	37.0	39.0	34.0	39.0
11	37.23756980043446	39.0	37.0	39.0	34.0	39.0
12	36.964198261305185	39.0	35.0	39.0	34.0	39.0
13	36.706460292028176	39.0	35.0	39.0	33.0	39.0
14	37.72363994420284	40.0	36.0	41.0	33.0	41.0
15	37.90877682431236	40.0	36.0	41.0	33.0	41.0
16	37.845840908992756	40.0	36.0	41.0	33.0	41.0
17	37.87380426943292	40.0	36.0	41.0	34.0	41.0
18	37.814733559919326	39.0	36.0	41.0	33.0	41.0
19	37.69458828000501	39.0	36.0	41.0	33.0	41.0
20	37.79465176439087	39.0	35.0	41.0	34.0	41.0
21	37.73596109745933	39.0	35.0	41.0	34.0	41.0
22	37.83995024897108	40.0	35.0	41.0	34.0	41.0
23	37.86110739226009	40.0	35.0	41.0	34.0	41.0
24	37.762201310283174	39.0	35.0	41.0	34.0	41.0
25	37.551018557306534	39.0	35.0	41.0	33.0	41.0
26	37.583779955344994	39.0	35.0	41.0	33.0	41.0
27	37.59740534564441	39.0	35.0	41.0	33.0	41.0
28	37.52883789024543	39.0	35.0	41.0	33.0	41.0
29	37.52242035300773	39.0	35.0	41.0	33.0	41.0
30	37.38721156711422	39.0	35.0	41.0	33.0	41.0
31	37.3058479052312	39.0	35.0	41.0	33.0	41.0
32	37.21914637253674	39.0	35.0	41.0	33.0	41.0
33	37.158572767357796	39.0	35.0	41.0	33.0	41.0
34	37.01962833562943	39.0	35.0	41.0	33.0	41.0
35	36.99202774310849	39.0	35.0	41.0	33.0	41.0
36	36.90670386477394	39.0	35.0	41.0	32.0	41.0
37	36.83287195588051	39.0	35.0	41.0	32.0	41.0
38	36.738655944859275	39.0	35.0	41.0	32.0	41.0
39	36.6845603382379	39.0	35.0	41.0	32.0	41.0
40	36.54202277664293	39.0	35.0	41.0	31.0	41.0
41	36.40622665221353	39.0	35.0	41.0	31.0	41.0
42	36.52862195696018	39.0	35.0	41.0	31.0	41.0
43	36.48229562994217	39.0	35.0	41.0	31.0	41.0
44	36.38887857207637	39.0	35.0	41.0	31.0	41.0
45	36.36831740465466	39.0	35.0	41.0	31.0	41.0
46	36.21805806877907	38.0	35.0	40.0	31.0	41.0
47	36.08144139786572	38.0	35.0	40.0	30.0	41.0
48	36.050619080728815	38.0	35.0	40.0	30.0	41.0
49	36.09248422607351	38.0	35.0	40.0	31.0	41.0
50	35.958799929173885	38.0	35.0	40.0	30.0	41.0
51	34.82531860956239	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	7.0
11	7.0
12	6.0
13	7.0
14	6.0
15	10.0
16	20.0
17	19.0
18	51.0
19	104.0
20	231.0
21	363.0
22	627.0
23	782.0
24	1095.0
25	1434.0
26	1788.0
27	2141.0
28	2345.0
29	2661.0
30	3341.0
31	4364.0
32	5699.0
33	8310.0
34	17525.0
35	25966.0
36	15949.0
37	22783.0
38	41726.0
39	72130.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03055024119748	20.72743605135757	24.04244384654917	13.199569860895776
2	31.313781294131367	27.04089344556106	24.913086852685996	16.73223840762158
3	30.353741907900133	26.837916157424	26.341701467914476	16.46664046676139
4	27.939175912210164	23.98025506039654	29.31942147154215	18.761147555851142
5	26.985182657965996	28.67118974921508	24.731702893074157	19.61192469974477
6	23.7690723074199	35.50461449430584	26.548997421756575	14.177315776517688
7	75.68591208060357	6.116958104623996	12.924902722054993	5.272227092717434
8	75.9834681476811	8.244332830928556	10.062059226181479	5.710139795208873
9	70.66503133191969	7.024309769253691	12.257237004055227	10.053421894771391
10	42.87744058595656	22.137912270624867	19.667635487339833	15.317011656078739
11	34.78944345355059	23.221897362590855	23.730204316074506	18.25845486778405
12	31.25072877483773	20.56548608741843	29.46884730493667	18.714937832807177
13	23.167914041277808	28.551130842614864	31.217906915479393	17.063048200627932
14	18.752942091011562	33.349600307489	28.818456249757073	19.079001351742367
15	16.56726537768891	25.706857609273037	39.942475372808815	17.783401640229236
16	18.34353258217341	24.06792397420893	35.55902968218939	22.02951376142827
17	18.590560260501913	23.06944846320281	30.29328058802952	28.04671068826575
18	19.937983960475574	25.057330287234457	32.40899491693047	22.59569083535951
19	22.893246902437024	25.400664210785433	28.92469542610115	22.781393460676387
20	25.306949164985987	25.465012329790586	30.04107051085497	19.18696799436846
21	22.091270681010393	28.216434250473977	29.826432825314292	19.86586224320134
22	20.11159432181833	25.385117014247278	28.615910828190522	25.88737783574387
23	19.486251527727994	28.57617910370412	27.970270305286476	23.96729906328141
24	20.576714618251547	24.992982168229304	30.954036440901216	23.476266772617933
25	18.395356570633936	28.482032191334167	29.261119484524063	23.861491753507835
26	17.142943516171243	29.453731974969017	30.432341623731933	22.97098288512781
27	19.690956282147067	29.88775787832591	29.40104425336748	21.020241586159543
28	16.894188371560723	27.813934606763898	33.4476340189935	21.84424300268189
29	19.696570547563624	26.067466195644194	31.583266034126094	22.652697222666085
30	20.121959119510436	27.486579746321578	32.329531467957665	20.061929666210325
31	22.563300842571678	28.095079744162245	25.75824973116306	23.58336968210302
32	24.640579046697734	30.23584233415244	24.953682310313404	20.16989630883642
33	23.360958398293263	27.10480969799571	25.37907088226022	24.155161021450812
34	20.749893113023802	29.275371081350706	26.66689699550427	23.307838810121225
35	19.697434280704634	28.741584000207297	27.800114876507752	23.760866842580317
36	22.515363653245693	27.043484644984083	27.072419705207878	23.368731996562342
37	20.10770752268379	27.293967255876623	31.87563970235756	20.722685519082024
38	21.63392398284626	27.399774565650198	25.978933548690797	24.987367902812746
39	23.6015080780642	27.191614878667085	27.17218088299439	22.034696160274322
40	22.716613475100733	26.795593233514577	28.572724171140084	21.91506912024461
41	17.629657141129677	28.721718137964096	26.87030615021183	26.7783185706944
42	20.853109223374346	27.092717434021584	28.351176620471342	23.70299672213273
43	22.260130510077605	24.59393745708326	27.514651073404362	25.631280959434772
44	20.882476150168642	26.257919353236623	28.19613652166027	24.663467974934463
45	20.69677352485176	27.6468022439787	25.900333832859	25.756090398310537
46	23.80362163306025	28.77570145927714	26.498900899578064	20.921776008084542
47	19.02026749815377	27.099195432579148	31.427794068744518	22.45274300052256
48	20.796534702638272	25.52849671565473	28.72128627139359	24.953682310313404
49	21.116547831382015	22.28604250430787	32.0177238040535	24.579685860256614
50	20.75507551186985	24.137454492060133	29.66664219422767	25.440827801842342
51	19.295798370135564	25.654169887671507	26.609458741627183	28.440573000565745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	243.0
1	381.0
2	519.0
3	822.5
4	1126.0
5	806.0
6	486.0
7	515.0
8	544.0
9	544.0
10	544.0
11	600.5
12	657.0
13	641.5
14	626.0
15	687.0
16	748.0
17	658.0
18	568.0
19	638.0
20	708.0
21	765.5
22	823.0
23	893.0
24	963.0
25	1229.5
26	1625.0
27	1754.0
28	2108.5
29	2463.0
30	2869.0
31	3275.0
32	4037.5
33	4800.0
34	5300.5
35	5801.0
36	6350.0
37	6899.0
38	7371.0
39	7843.0
40	9502.0
41	11161.0
42	14002.5
43	16844.0
44	18449.5
45	20055.0
46	24794.0
47	29533.0
48	27242.5
49	24952.0
50	24490.5
51	24029.0
52	20188.5
53	16348.0
54	13605.0
55	10862.0
56	9748.0
57	8634.0
58	7959.5
59	7285.0
60	6343.0
61	5401.0
62	4886.5
63	4372.0
64	3614.5
65	2857.0
66	2441.5
67	2026.0
68	1728.5
69	1431.0
70	1289.5
71	1148.0
72	902.5
73	657.0
74	665.5
75	466.5
76	259.0
77	171.0
78	83.0
79	45.5
80	8.0
81	9.0
82	10.0
83	20.5
84	31.0
85	17.5
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	231553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.48241223391621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60863668997793	23.909860809404325
2	10.778578456138485	7.002284574157969
3	3.7998245007578375	3.7028239755045282
4	1.8945940915255137	2.461639451874949
5	1.1221315180684448	1.8224769275284707
6	0.7325763820565321	1.4277508820874703
7	0.5916451724413009	1.3452643671211342
8	0.5637248384609248	1.4648914071508468
9	0.4041800728587763	1.1815869368999754
>10	6.260802510170979	45.40299629026616
>50	0.19810141728933442	3.9597845849546323
>100	0.03589757226048342	2.3182597504674956
>500	0.003988619140053713	0.9889744464550232
>1k	0.005318158853404951	3.0114055961270205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	2270	0.9803371150449358	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	2106	0.9095109974822179	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	1496	0.6460723894745479	RNA PCR Primer, Index 30 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1101	0.47548509412531903	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	979	0.422797372523785	RNA PCR Primer, Index 30 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	712	0.3074889981991164	RNA PCR Primer, Index 30 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.2586880757321218	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCC	493	0.21291021925865786	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	414	0.17879276018881207	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	368	0.1589268979456107	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	359	0.1550400988110713	No Hit
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	309	0.13344677028585247	Illumina Single End Adapter 2 (95% over 22bp)
AGTGGGGGTACTTGATGTAAGTGAAGAGCAGACACTTCCCAAATGGTTTCT	271	0.11703584060668616	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	266	0.11487650775416428	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	266	0.11487650775416428	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3221724615962652	0.0
2	0.0	0.0	0.0	1.5309669924380163	0.0
3	0.0	0.0	0.0	2.028909148229563	0.0
4	4.3186657050437696E-4	0.0	0.0	2.747535121548846	0.0
5	4.3186657050437696E-4	0.0	0.0	4.880092246699459	0.0
6	4.3186657050437696E-4	0.0	0.0	5.726118858317534	0.0
7	4.3186657050437696E-4	0.0	0.0	6.70256917422793	0.0
8	4.3186657050437696E-4	0.0	0.0	7.963187693530207	0.0
9	4.3186657050437696E-4	0.0	0.0	8.413624526566272	0.0
10	4.3186657050437696E-4	0.0	0.0	10.281879310568208	0.0
11	4.3186657050437696E-4	0.0	0.0	11.851714294351616	0.0
12	4.3186657050437696E-4	0.0	0.0	14.048187671936878	0.0
13	4.3186657050437696E-4	0.0	0.0	14.600545015611976	0.0
14	4.3186657050437696E-4	0.0	0.0	14.826411231985766	0.0
15	4.3186657050437696E-4	0.0	0.0	15.375745509667333	0.0
16	4.3186657050437696E-4	0.0	0.0	16.227386386701966	0.0
17	4.3186657050437696E-4	0.0	0.0	17.21074656774043	0.0
18	4.3186657050437696E-4	0.0	0.0	18.47352441989523	0.0
19	4.3186657050437696E-4	0.0	0.0	19.31739169866078	0.0
20	4.3186657050437696E-4	0.0	0.0	19.981170617526008	0.0
21	4.3186657050437696E-4	0.0	0.0	20.732618450203624	0.0
22	4.3186657050437696E-4	0.0	0.0	21.51472880938705	0.0
23	4.3186657050437696E-4	0.0	0.0	22.274382106904252	0.0
24	4.3186657050437696E-4	0.0	0.0	22.8712217073413	0.0
25	4.3186657050437696E-4	0.0	0.0	23.462015175791286	0.0
26	4.3186657050437696E-4	0.0	0.0	24.00012092263974	0.0
27	4.3186657050437696E-4	0.0	0.0	24.512314675257933	0.0
28	4.3186657050437696E-4	0.0	0.0	24.974411905697615	0.0
29	4.3186657050437696E-4	0.0	0.0	25.505607787418	0.0
30	4.3186657050437696E-4	0.0	0.0	26.102015521284542	0.0
31	4.3186657050437696E-4	0.0	0.0	26.645735533549555	0.0
32	4.3186657050437696E-4	0.0	0.0	27.18600061325053	0.0
33	4.3186657050437696E-4	0.0	0.0	27.66062197423484	0.0
34	4.3186657050437696E-4	0.0	0.0	28.19743212137178	0.0
35	4.3186657050437696E-4	0.0	0.0	28.776133325847645	0.0
36	4.3186657050437696E-4	0.0	0.0	29.298691876157942	0.0
37	4.3186657050437696E-4	0.0	0.0	29.839820688999925	0.0
38	4.3186657050437696E-4	0.0	0.0	30.295871787452548	0.0
39	4.3186657050437696E-4	0.0	0.0	30.793813943244096	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAA	60	0.0	45.000004	5
GATTGCG	30	2.1579708E-6	45.000004	1
GTTGACG	25	3.8811286E-5	45.0	1
CGGGTTC	20	7.021608E-4	45.0	6
AGGTATA	20	7.021608E-4	45.0	6
AGACTGG	20	7.021608E-4	45.0	2
GTCGATG	25	3.8811286E-5	45.0	1
ATTAGCG	25	3.8811286E-5	45.0	1
TTGTGCG	20	7.021608E-4	45.0	1
GCGCGAC	20	7.021608E-4	45.0	9
GGCACCG	25	3.8811286E-5	45.0	8
GGCGTAG	20	7.021608E-4	45.0	1
TAGAACG	20	7.021608E-4	45.0	1
CCGTGGC	20	7.021608E-4	45.0	27
CGTCTAC	20	7.021608E-4	45.0	35
CGATTGA	25	3.8811286E-5	45.0	10
AAGCGGC	20	7.021608E-4	45.0	22
TACGGGT	20	7.021608E-4	45.0	4
GGATAGT	20	7.021608E-4	45.0	8
CGACAGG	35	1.2063174E-7	45.0	2
>>END_MODULE